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Grade 9 Science Unit 1: Reproduction Notes Ch. 4 – Nucleus Section 4.1 p. 112 The nucleus is an organelle that controls functions and heredity (passing of traits from parent to offspring) within the cell. It determines what traits will be expressed by the organism. The information is carried on long, two-stranded molecules called deoxyribonucleic acid (DNA). It has a twisted ladder shape or helix. The rungs of the molecule are composed of 4 nitrogen bases called cytosine, guanine, thymine and adenine, while the sides are composed of phosphate and sugar. Adenine and thymine pair as do cytosine and guanine. The DNA forms a loosely coiled mass (chromatin) but when the cell is ready to divide, it becomes a tight, x-shaped structure called a chromosome. These are found in pairs and species have different numbers of pairs. Humans have 23 pairs, one from each parent. Small sections of DNA on the chromosomes, called genes, contain coded information to produce a particular trait. Depending on the part of the gene a cell ‘reads’ it will become a particular kind of cell such as muscle or bone. Show Bill Nye: Genes Section 4.2 p.122 Sometimes the order of the bases in a gene changes. This is a mutation and may change the trait determined by the gene. The bases may be deleted, added or substituted for another. The mutation can have positive, negative, or neutral effects on the organism’s ability to survive. Mutagens are substances or factors in the environment that cause mutations in DNA. Researchers are working to treat mutated genes with techniques called gene therapy. One method is to inject a healthy gene into cells to replace the defective one. Complete p.127 #s 2, 3; p. 131 #s 1, 3 – 7, 14; p. 132-33 #s 2-7, 9, 10, 12,13,16, &19. Chapter 5: Mitosis Section 5.1 p. 136 There are three stages in the life or cell cycle of cells: interphase, mitosis (meiosis in sex cells) and cytokinesis. Interphase The longest stage in the cell cycle, interphase is a time when basic cell functions are carried out and eventually, the cell doubles all material and organelles in the cytoplasm. DNA replication occurs during this time when the DNA is a loosely coiled form. Mitosis This is the shortest stage in which the contents of the cell’s nucleus divide. It is divided into 3 steps (see diagrams p. 142-43): (pigs munch at two) 1. Prophase a) Early prophase Replicated chromosomes pair up into x-shaped structures. Nuclear membrane begins to break down Spindle fibres begin to form from the centrioles b) Late prophase Spindle is completed and attached to the centromeres Nuclear membrane completely disintegrated 2. Metaphase Spindle fibres pull chromosomes into a line across the equator of the cell 3. Anaphase Spindle fibres shorten, pulling the sister chromatids apart and to opposite poles of the cell. When separate each is considered a single chromosome. 4. Telophase A complete set of chromosomes is at each pole. Spindle breaks down Nuclear membrane forms around each set of chromosomes Now the cell contains 2 nuclei and 2 sets of organelles etc. Cytokinesis The final stage, cytokinesis, separates the two nuclei into two daughter cells which are identical to the parent cell. In animal cells the cell membrane pinches together to divide the cytoplasm and organelles. In plant cells, a cell plate forms to divide the cell. Check points are stages of the cell cycle when special proteins monitor cell activities to determine whether the cell is fit to replicate and sends this information to the nucleus. It will not replicate if the cell lacks sufficient nutrients for growth, the DNA has not replicated or the DNA is damaged. If a mutation occurs in the gene producing the instructions for a checkpoint protein, cell cycle control will be lost. Cancer results when cells divide uncontrolledly. Cancer cells are not specialized so they do not function as a part of the body. Complete p. 141 #s 2, 6; p. 146 #s 3, 4, 5; p. 151 #s 2, 3, 5, 6, 8, 10-12. Section 5.2 p. 152 Through asexual reproduction, a single parent produces offspring that are genetically identical to itself; clones. This type of reproduction occurs in many different ways by many different kinds of species. 1. Binary fission is carried out by many unicellular organisms such as amoebas and bacteria. In this form the parent replicates its DNA and other cellular materials and divides into two parts (mitosis) which are the offspring. This type of reproduction can result in extremely fast reproduction. Ex. bacterium 2. Budding occurs when a growth develops on an organism which grows into a complete individual. The bud may or may not detach from the parent. Ex. Hydra, or yeast 3. Fragmentation is practiced by some animals and many plants such as starfish or Japanese knotweed. The organism breaks apart due to injury and if the parts contain enough genetic information both grow into a complete organism. 4. Vegetative Propagation/ Reproduction can be seen in many types of plants. It occurs when special cells, usually in stems or roots divide repeatedly to form a new plant. Tulips bulbs and strawberry runners are examples. Humans have devised methods of carrying this out as well, such as cuttings and grafting. 5. Spore formation occurs in some bacteria, and fungi. Single-celled reproductive cells called spores develop and when conditions are suitable, the spores grows into a new organism. Ex. Rhizopus, mushrooms Advantages and Disadvantages of Asexual Reproduction Advantages Large numbers of offspring formed quickly Large colonies outcompete other species for nutrients/water Disadvantages Large numbers produced in the same area means that they must compete with one another for resources Offspring are genetic clones which means that disease or environmental changes can wipe out whole colonies if it cannot adapt to these new conditions – no genetic diversity! Large numbers mean that species may survive environmental change such as predator increases or temperature drops Energy is not expended to find a mate Complete p. 165 #s 1, 3-6, 11-14. Review p. 166-67 #s 1 – 11, 16, 18 – 20. WHMIS (workplace hazardous materials information system) Symbols Laboratory Write-up Format Title page containing course name/ grade, student name, teacher name, date due and lab title and page. Purpose Observation table or results Questions for analysis Ch. 6 Meiosis Section 6.1p. 170 Meiosis is a type of cell division that results in daughter cells, called gametes. Since cell division occurs twice (meiosis l & meiosis ll), the gametes contain half the total number of chromosomes (haploid, n) as other body cells (diploid, 2n). After fertilization, offspring get a randomly selected half of each parent’s DNA thus creating a genetically unique organism (except identical twins). Meiosis in males produces 4 haploid sperm, while in females one of the 4 haploid cells contains most of the cytoplasm and is the only survivor. Complete p. 175 #s 1 – 4; p. 179 #s 1, 3 – 7, 10, 12, & 16. Section 6.2 p. 180 Sexual Reproduction Sexual reproduction, composed of 3 stages, mating, fertilization and development, results in an increase of variation in the species. The sperm and egg unite either internally or externally. External fertilization is the fusion of eggs and sperm outside the body. Advantages: Little energy is used in finding a mate. Large numbers of offspring are produced meaning that in an environmental change some individuals will survive. Offspring are widely spread out, so do not compete with the parents or one another for resources. Little chance of offspring and parent reproducing, maintaining genetic variation. Ex. fish such as salmon, mosses Disadvantages: Many gametes do not survive outside the body Gametes may not meet. Zygotes are unprotected and often preyed upon. Usually no parental care so few offspring survive to adulthood. Internal fertilization is the fusion of gametes inside the female(usually). Advantages: Gametes usually meet Developing embryo protected increases survival rate Parental care after birth increases survival Disadvantages: A great deal of energy expended in finding a mate, mating Energy expended caring for the young Fewer zygotes produced Complete p. 191 #s 1- 3. Pollination in Flowering Plants p. 188 Through pollination, in flowering plants, male gametes, called pollen, is transferred from the anthers to the eggs in the ovules. Pollen transport is often achieved through insect activity, fruit development or winged structures(cone-bearing) which are carried by wind. Some plants do not produce flowers but cones which either produce pollen or eggs. Ex. alder, black spruce (p. 190). Insects often undergo a massive physical and functional change called metamorphosis in their lifecycles. It may be complete with the egg, larva, pupa and adult stages or incomplete consisting of egg, nymph and adult phases (see p. 192-3). Advantages & Disadvantages Sexual Reproduction Advantages Little energy expenditure in mating(external reproduction) Great numbers of offspring (external) Protection of embryo and parental care of offspring(internal) Offspring genetically unique, greater variation Table 6.1 P. 194 Disadvantages Greater energy consumption (internal reproduction) Fewer offspring(internal) Gametes, embryos and offspring are unprotected and preyed upon(external) Some beneficial traits may not be inherited or maladaptive ones may be inherited Sexual vs Asexual Reproduction Asexual Reproduction Number of parent cells 1 Gametes(eggs or sperm) None – parent cell divides Variation in offspring Amount of energy required Meiosis or mitosis Very little (mutation), much less than sexual Very little – no mating, or parental care of offspring mitosis Sexual Reproduction 2 2 - cells unite to form zygote Great genetic diversity Energy required for mating and parental care of offspring meiosis Complete p. 191 #s 4, 5: p. 195 #s 1, 2, 4- 10, 12-17. Section 6.3 Human Reproductive Systems p. 196 The male reproductive system functions to produce gametes – sperm and deliver them to the egg’s environment for fertilization. See p. 196 table 6.2 for the structures and their functions. The female reproductive system produces gametes, eggs and houses the developing embryo. See table 6.3 for structures and functions. Human development begins with a fertilized zygote which travels down the fallopian tube to the uterus where it divides and undergoes differentiation for about 38 weeks, becoming an embryo and then fetus. See table 6.4 p. 199 for details. Complete p. 200 #s 1 – 4; read p. 201; complete p. 203 #s 1 – 12. Section 6.4 Studying Genetic Changes p. 204 The understanding of genetics has evolved. Gregor Mendel’s experimentation with pea plants in the 1800s provided scientific explanation for heredity through dominant and recessive factors (genes). Watson and Crick’s model of DNA helped explain how and why mutations occur. The Human Genome Project (HGP) has created greater insights into how and why certain medical conditions occur and how to treat them. It has also introduced the concept of genetically engineering people and related ethical issues. Genetic engineering has applications in many areas such as food production and health. Complete p. 211 #s 1 – 3, 7, 9 – 11, 14 & 15. Chapter Review: Complete p. 212-213 #s 3, 5, 7, 9 & 15 Review STSE article ‘Heredity, Genetics and Genetic Engineering’ Other available practice questions: Chapter Review p. 212 -221 and Review handout