* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Love Sandhu
Saethre–Chotzen syndrome wikipedia , lookup
Oncogenomics wikipedia , lookup
Gene nomenclature wikipedia , lookup
Genome (book) wikipedia , lookup
Public health genomics wikipedia , lookup
Gene expression programming wikipedia , lookup
Gene expression profiling wikipedia , lookup
Nutriepigenomics wikipedia , lookup
Therapeutic gene modulation wikipedia , lookup
Site-specific recombinase technology wikipedia , lookup
Neuronal ceroid lipofuscinosis wikipedia , lookup
Biology and consumer behaviour wikipedia , lookup
Frameshift mutation wikipedia , lookup
Designer baby wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Point mutation wikipedia , lookup
Epigenetics of neurodegenerative diseases wikipedia , lookup
Department of Molecular and Cellular Biology Graduate Seminar MCB*6500 Friday, March 10, 2017 in SSC 1511 @ 12 noon presented by: Love Sandhu Phenotypic classification of cardiac actin knockouts in zebrafish using the CRISPR-Cas9 system Cardiomyopathy is a common cause of heart failure, a growing epidemic in Canada. Two prevalent forms of cardiomyopathy are hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM), which are characterized by changes to the heart muscle (myocardium). The development of HCM and DCM has been associated with mutations found in genes encoding muscle proteins, including cardiac actin (ACTC1). Anatomical and histological descriptions of cardiomyopathy are largely determined, but our understanding of the molecular basis of cardiomyopathy development remains incomplete. To address this gap in understanding, an in vivo model is required to study the underlying molecular mechanisms leading to the development of cardiomyopathy. Generating a model for cardiovascular disease will allow scientists to target the associated dysfunctions with precision therapies leading to improved treatments and reducing the global burden of heart failure. I will take the first step toward our overall goal of using zebrafish to determine connections between cardiac actin mutations and cardiomyopathy development by using CRISPR-Cas9 system to produce and characterize zfactc knockout lines. This work will clarify which zfactc gene(s) is a cardiac isoform and will be the first characterization of the putative actc1c gene. I hypothesize that a cardiac phenotype should be observed if the knocked out zebrafish gene encodes for cardiac actin. This work will provide a zfactc knockout background for future human ACTC rescue experiments, including the characterization of cardiomyopathy-related ACTC mutants.