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Job advertisement IGB – INSTITUTE OF GENETICS AND BIOPHYSICS “A.BUZZATI TRAVERSO” of the National Research Council based in Napoli, Italy offers 2 pre-doc positions (Early Stage Researcher) within the DISCHROM project funded by FP7/People - Marie Curie Actions Job Reference Number IGB -01 Main Research Field Biological sciences, Biology Job Summary Job Description Understanding the role of MeCP2 in neuronal differentiation . The aim of our project is to analyze of the role of MeCP2 in heterochromatin condensation, nuclear factor relocalisation and chromosome territory repositioning before and after neuronal differentiation of embryonic stem cells from wild type and Mecp2-/y mice. Also, we aim to identify MECP2 genomic binding regions and its biochemical partners before and after neuronal differentiation. This may be instrumental to understand the role of MECP2 in neurons and of value to understand MECP2 dysregulation in Rett Syndrome. By three-dimensional DNA Fluorescence In Situ Hybridization (FISH), the PhD student will study heterochromatin consensation nuclear factor relocalisation and chromosome territory repositioning before and after neuronal differentiation of embryonic stem cells from wild type and Mecp2-/y mice. Using different approaches (ChIP on chip and ChIP seq), the PhD student will define the map of in vivo binding sites for wt and mutated MECP2 protein identifying new MECP2 specific target genes before and after neuronal differentiation of embryonic stem cells. Through in vivo biotinylation tagging technique for the purification of MECP2 protein complexes, the PhD student will also analyze any abnormal protein-potein interactions of mutated MECP2 with respect to wild-type protein. Job Reference Number IGB -02 Main Research Field Biological sciences, Biology Job Summary Job Description The aim of our job is to study the alterations of the complex epigenetic network underlying ICF syndrome pathogenesis. This human genetic disease, due to mutations in the DNMT3B gene, is characterised by inheritance of aberrant patterns of DNA methylation and heterochromatin defects. We are interested in understanding why the impaired DNMT3B activity mainly leads to an immune-specific phenotype in ICF syndrome and to what extent the activity of DNMT3B on its specific genomic targets in lymphocytes is altered. In addition we would like to test the hypothesis that heterochromatin defects in ICF lymphoblastoid cells would compromise its in trans role of gene expression regulation. Using different approaches (ChIP on chip and ChIP seq), the PhD student will define the map of in vivo binding sites for DNMT3B protein identifying new DNMT3B specific target genes in human lymphoblastoid cell lines (LCLs). This will be compared to the binding sites of mutated DNMT3B protein in ICF LCLs. Through in vivo biotinylation tagging technique for the purification of DNMT3B protein complexes, the PhD student will analyze abnormal protein-potein interactions of mutated DNMT3B with respect to wild-type protein. Finally, by threedimensional DNA Fluorescence In Situ Hybridization (FISH), it will be studied the nuclear localization of dysregulated genes in the B cells of normal and ICF individuals relative to domains of hypomethylated and undercondensed pericentromeric heterochromatin. Application and position details: - The type of contract offered is a full-time (40 hours per week) temporary contract. - The start date of the contract is 01/04/2010. - The contract duration is 12 months, renewable annually (up to 36 months). The place where the work has to be carried out is the premises of Institute of Genetics and Biophysics “A.Buzzati Traverso” – CNR located in Napoli (address: Via P.Castellino, 111, 80125, Napoli), Italy. Benefit: - Excellent work environment - Competitive salary - Training in science and complementary skills Requirements: The applicant shall have: - Master’s degree (or any title allowing the candidate to enter a PhD program in Italy or the country where the degree was obtained) in biological or medical science. - Fluency in English (written and spoken) with an excellent comprehension level. - Two years of experience in biological science. - Proficiency in molecular and cellular biology as well as Knowledge of chromatin-related and imaging techniques would be an advantage. The candidates must meet the mobility requirements of the Marie Curie Action: 1. Researchers can be nationals of any country other than Italy where they will carry out their project 2. Researchers must not have resided or carried out their main activity in Italy for more than 12 months in the 3 years immediately prior to their recruitment -Applications from female candidates are encouraged. Further information on the scientists responsible of research projects and on the Institute at www.igb.cnr.it Applicants will send their CV (including an authorisation for personal data treatment ) by the 15/02/2009 to: IGB- 01: Maurizio D’Esposito - [email protected] IGB -02: Maria Matarazzo - [email protected]