Download Job description-IGB 01-02

Job advertisement
IGB – INSTITUTE OF GENETICS AND BIOPHYSICS “A.BUZZATI TRAVERSO” of the National
Research Council based in Napoli, Italy offers 2 pre-doc positions (Early Stage Researcher)
within the DISCHROM project funded by FP7/People - Marie Curie Actions
Job Reference Number
IGB -01
Main Research Field
Biological sciences, Biology
Job Summary
Job Description
Understanding the role of MeCP2 in
neuronal differentiation .
The aim of our project is to analyze of the
role
of
MeCP2
in
heterochromatin
condensation, nuclear factor relocalisation
and chromosome territory repositioning
before and after neuronal differentiation of
embryonic stem cells from wild type and
Mecp2-/y mice.
Also, we aim to identify MECP2 genomic
binding regions and its biochemical partners
before and after neuronal differentiation. This
may be instrumental to understand the role
of MECP2 in neurons and of value to
understand MECP2 dysregulation in Rett
Syndrome.
By three-dimensional DNA Fluorescence In
Situ Hybridization (FISH), the PhD student
will study heterochromatin consensation
nuclear factor relocalisation and chromosome
territory repositioning before and after
neuronal differentiation of embryonic stem
cells from wild type and Mecp2-/y mice.
Using different approaches (ChIP on chip and
ChIP seq), the PhD student will define the
map of in vivo binding sites for wt and
mutated MECP2 protein identifying new
MECP2 specific target genes before and after
neuronal differentiation of embryonic stem
cells.
Through
in
vivo
biotinylation
tagging
technique for the purification of MECP2
protein complexes, the PhD student will also
analyze
any
abnormal
protein-potein
interactions of mutated MECP2 with respect
to wild-type protein.
Job Reference Number
IGB -02
Main Research Field
Biological sciences, Biology
Job Summary
Job Description
The aim of our job is to study the alterations
of the complex epigenetic network underlying
ICF syndrome pathogenesis. This human
genetic disease, due to mutations in the
DNMT3B gene, is characterised by inheritance
of aberrant patterns of DNA methylation and
heterochromatin defects. We are interested
in understanding why the impaired DNMT3B
activity mainly leads to an immune-specific
phenotype in ICF syndrome and to what
extent the activity of DNMT3B on its specific
genomic targets in lymphocytes is altered. In
addition we would like to test the hypothesis
that
heterochromatin
defects
in
ICF
lymphoblastoid cells would compromise its in
trans role of gene expression regulation.
Using different approaches (ChIP on chip and
ChIP seq), the PhD student will define the
map of in vivo binding sites for DNMT3B
protein identifying new DNMT3B specific
target genes in human lymphoblastoid cell
lines (LCLs). This will be compared to the
binding sites of mutated DNMT3B protein in
ICF LCLs. Through in vivo biotinylation
tagging technique for the purification of
DNMT3B protein complexes, the PhD student
will
analyze
abnormal
protein-potein
interactions of mutated DNMT3B with respect
to wild-type protein. Finally, by threedimensional DNA Fluorescence In Situ
Hybridization (FISH), it will be studied the
nuclear localization of dysregulated genes in
the B cells of normal and ICF individuals
relative to domains of hypomethylated and
undercondensed
pericentromeric
heterochromatin.
Application and position details:
- The type of contract offered is a full-time (40 hours per week) temporary contract.
- The start date of the contract is 01/04/2010.
- The contract duration is 12 months, renewable annually (up to 36 months).
The place where the work has to be carried out is the premises of Institute of Genetics and
Biophysics “A.Buzzati Traverso” – CNR located in Napoli (address: Via P.Castellino, 111,
80125, Napoli), Italy.
Benefit:
- Excellent work environment
- Competitive salary
- Training in science and complementary skills
Requirements:
The applicant shall have:
- Master’s degree (or any title allowing the candidate to enter a PhD program in Italy or the
country where the degree was obtained) in biological or medical science.
- Fluency in English (written and spoken) with an excellent comprehension level.
- Two years of experience in biological science.
- Proficiency in molecular and cellular biology as well as Knowledge of chromatin-related and
imaging techniques would be an advantage.
The candidates must meet the mobility requirements of the Marie Curie Action:
1. Researchers can be nationals of any country other than Italy where they will carry out their
project
2. Researchers must not have resided or carried out their main activity in Italy for more than
12 months in the 3 years immediately prior to their recruitment
-Applications from female candidates are encouraged.
Further information on the scientists responsible of research projects and on the Institute at
www.igb.cnr.it
Applicants will send their CV (including an authorisation for personal data treatment ) by the
15/02/2009 to:
IGB- 01: Maurizio D’Esposito - [email protected]
IGB -02: Maria Matarazzo - [email protected]