Download The mitochondrial gene ATPase 6/8, an alternative for genetic

58º Congresso Brasileiro de Genética
Resumos do 58o Congresso Brasileiro de Genética • 11 a 14 de setembro de 2012
Rafain Palace Hotel e Convention Center • Foz do Iguaçu • PR • Brasil
www.sbg.org.br - ISBN 978-85-89109-06-2
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The mitochondrial gene ATPase 6/8,
an alternative for genetic studies at
Macrobrachium freshwater prawns, with
emphasis at relationship between populations
of M. amazonicum: is yet a single species?
Carvalho, SC; Iketani, G; Pimentel, L; Carneiro, J; Gomes, FNP; Maciel, C; Schneider, H; Sampaio, I
Instituto de Estudos Costeiros, UFPA, Bragança, PA.
[email protected]
Keywords: ATPase 6/8, Macrobrachium, Macrobrachium amazonicum.
The freshwater prawn of the genus Macrobrachium (Bate, 1868) have a worldwide distribution in tropical and
subtropical regions encompassing the majority of shrimp that have favorable conditions for aquaculture. In Brazil,
18 species are found; three of them have significant economic value: M. amazonicum, M. acanthurus and M. carcinus.
The great majority of molecular studies involving prawn of this genus is restrict to just one mitochondrial gene,
the cytochrome oxidase subunit I (COI). Although not yet found in this group, previous studies had shown the
existence of a pseudogene in COI for a great number of crustaceans groups, making this a not good region for
genetic inferences. This study has the primary goal testing the utility of mitochondrial genes ATPase 6 and 8 for
genetic inferences in Macrobrachiun prawns, checking the interspecific variation between M. amazonicum, M.
acanthurus and M. carcinus and the intraspecific populational variation between populations of M. amazonicum
from Pantanal and Amazonian regions. Recent studies had suggested a possible speciation process between these
two populations. In our analysis, we obtain a fragment with 785 bp of ATPase 6/8. None of the sequences obtained
in the present study shown any signs of presence of pseudogenes. The genetic interspecific variation show a
slight variation values from 17.2% (M. amazonicum vs M. acanthurus) to 23.6% (M. acanthurus vs M. carcinus).
The intraspecific variation between the two populations of M. amazonicum ranges between 2.5 to 3.3% (media
of 1.4%). The interspecific variation of ATPase 6/8 presents values much higher than the previous studies with
COI gene; however, in the case of intraspecific variation, the values are similar between the two genes. The present
study showed that the mitochondrial genes ATPase 6 and 8 can be a viable alternative to COI gene (mainly for
populational inferences). The results shown’s here confirm the previous results from other studies suggesting the two
population of M. amazonicum from Pantanal and Amazon regions are different populations and not different species.
Financial Support: CNPq, UFPA.