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Biomolecules carbonylation in oxidative stress related human diseases » Dr. Maria Fedorova Keywords Lipid carbonylation Lipid derived reactive carbonyls are characterized by large variety of species with different physicochemical properties. New high throughput analytical methods for sensitive and specific detection of lipid peroxidation products were developed and allowed simultaneous detection of different types of carbonyls using shotgun- and LCMS-based lipidomics approach. Oxidative stress related human disorders Protein and lipid oxidation Carbonyl stress Protein carbonylation Protein carbonylation results either from direct oxidation of amino acid residues or from the reaction between proteins and secondary products of glycation and lipidperoxidation. Detection of carbonyated proteins in vivo is challenged by their extremely low abundance. New method utilizing MS-based enrichment was developed and tested for specific detection of carbonylated proteins in complex biological samples. Additionally combination of oxolipidomics and proteomics techniques allow to map adducts between proteins and lipid peroxidation products. Contact Selected References Dr. Maria Fedorova Bioanalytics UNIVERSITÄT LEIPZIG BOLLINENI, R. C.; HOFFMANN, R.; FEDOROVA, M. Identification of protein carbonylation sites by tow-dimensional liquid chromatography in combination with MALDI- and ESI-MS. J Proteomics. 74 (2011), 2338–2350. FEDOROVA, M.; KULEVA, N.; HOFFMANN, R. Identification, quantification and functional aspects of skeletal muscle protein-carbonylation in vivo during acute oxidative stress. J. Proteome Res. 9 (2010), 2516–2526. FEDOROVA, M.; TODOROVSKY, T.; KULEVA, N.; HOFFMANN, R. Quantitative evaluation of tryptophan oxidation in actin and troponin I from skeletal muscles using a rat model of acute oxidative stress. Proteomics 10 (2010), 2696–2700. Fakultät für Chemie und Mineralogie Institut für Bioanalytische Chemie Biotechnologisch-Biomedizinisches Zentrum Deutscher Platz 5 04103 Leipzig fon +49 341 97-31336 fax +49 341 97-31339 [email protected] PROTEIN ENGINEERING AND BIOANALYTICS Carbonyl stress Oxidative stress (OS) related human disorders, such as diabetes, obesity, metabolic syndrome and neurodegeneration, are characterized by elevated levels of oxidatively modified biomolecules (protein, lipids and DNA). Oxidation of biomolecules results in numerous functional dysfunctions associated with disease progression. One of the most abundant and hazardous class of modifications are reactive carbonyls (aldehydes, ketones), which are considered as a major hallmark of OS-related diseases. Accumulation of reactive aldehydes and ketones leads to carbonyl stress, which is characterized by high in vivo toxicity due to protein aggregation, membrane disruption and mutagenesis. Sensitive and specific detection of carbonyls as well as determination of pathological threshold (control vs disease) will facilitate identification of prognostic biomarkers and development of preventive therapies.