Download HUMAN SMALL INTESTINE EPITHELIAL CELLS

REF: P10756
GASTROINTESTINAL CELL SYSTEM INNOPROFILETM
HUMAN SMALL INTESTINE EPITHELIAL CELLS
Product Type:
Cryo-preserved Epithelial Cells
Catalog Number:
P10756
Source:
Small Intestine
Number of Cells:
5 x 105 Cells / vial (1ml)
Storage:
Liquid Nitrogen
Intestine epithelial cells play important Cell Characterization functions in the maintenance of overall individual health. In addition to their primary roles in absorbing nutrients and in serving as a physical barrier between the outside world and the inside environment, intestine epithelial cells are crucial in maintaining homeostasis of the mammalian immune and endocrine system in the context of health and Characterization of small intestine cells by RT‐PCR. Whole small intestine cells (Lane 2), and small intestine epithelial cells (Lane 3) express the characteristic markers of stem cells, including PHLDA1, an epithelial stem cell marker; of epithelial cells (CK18, cytokeratin 18; CDH1, E‐
caherin); and of entero‐endocrine cells. Lane 1: No RT control. Lane 4: Brain tissue. disease. A failing intestine epithelium can lead to various disorders such as hormonal imbalance, inflammatory bowel disease (IBD), weight gain, and diabetes. Thus insights into the mechanisms of and remedies for those disorders can be gained from in vitro systems Small intestine epithelial cells secretes GLP1. Cells were incubated at 37°C, 2h, in RBB (PBS supplemented with 1.2mM MgCl2, 2.5mM CaCl2, and 0.5% BSA) containing various combinations of additives. Forskolin, IBMX, and tolbutamide were used at 10µM, each. GLP1 in the clarified supernatant was quantified by ELISA. The results indicate that cells secrete GLP1 in a dose dependent fashion in response to glucose. This GLP1 secretion is also activated by the combined actions of the potassium channel blocker, tolbutamide, and an elevated intracellular cAMP pool, induced by forskolin and IBMX. Error bar = ± Standard deviation. offered by the availability of human intestine epithelial cells. Inoprot is dedicated to offer scientists and researchers the highest quality cells, genomic and proteomic biological products. All products are validated under strict quality assurance and control parameters, providing customers with reliable, quality products for reproducible results with maximum impact. Unless specified, each product is from a single donor. INNOVATIVE TECHNOLOGIES IN BIOLOGICAL SYSTEMS, S.L.
Parque Tecnológico Bizkaia, Edf. 502, 1ª Planta | 48160 | Derio | Bizkaia
Tel.: +34 944005355 | Fax: +34 946579925
[email protected] | www.innoprot.com
Growth characteristics of small intestine epithelial cells. Growth curve: Cells were grown in Epithelial Pro‐
Conditioned Medium in vessels pre‐coated with Culture Vessel Coating Solution (CCS102), dissociated with Cell Dissociation Solution (CCS101) every 7 day period, and counted. The results indicate that small intestine epithelial cells proliferate in culture for at least four passages, totaling over 7 population doublings. B: Phase contrast photomicrograph: Cells maintain the typical epithelial morphology at passage 4. References 1.
Day (2006). Epithelial stem cells and tissue engineered intestines. Curr Stem Cell Res Ther. 1(1):113‐20. 2.
Fagerholm (2007). Prediction of human pharmacokinetics‐‐gut‐wall metabolism. J Pharm Pharmacol. 59(10):1335‐43. 3.
Hayashi (2007). Mechanistic analysis for drug permeation through intestinal membrane. Drug Metab Pharmacokinet. 22(2):67‐77. THESE PRODUCTS ARE FOR RESEARCH USE ONLY. Not approved for human or veterinary use, for application to humans or animals, or for use in vitro diagnostic or clinical procedures INNOVATIVE TECHNOLOGIES IN BIOLOGICAL SYSTEMS, S.L.
Parque Tecnológico Bizkaia, Edf. 502, 1ª Planta | 48160 | Derio | Bizkaia
Tel.: +34 944005355 | Fax: +34 946579925
[email protected] | www.innoprot.com