* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Replication PP
Survey
Document related concepts
Zinc finger nuclease wikipedia , lookup
DNA sequencing wikipedia , lookup
DNA repair protein XRCC4 wikipedia , lookup
Homologous recombination wikipedia , lookup
DNA profiling wikipedia , lookup
Eukaryotic DNA replication wikipedia , lookup
Microsatellite wikipedia , lookup
United Kingdom National DNA Database wikipedia , lookup
DNA nanotechnology wikipedia , lookup
DNA polymerase wikipedia , lookup
DNA replication wikipedia , lookup
Transcript
Do Now 3/7 • OBJECTIVES: – 1. Describe the structure of DNA. – 2. Describe DNA replication, and be able to determine the sequence of a complementary strand using basepairing rules. • TASK: 1. HW check - The Race to Discover DNA’s Structure Maurice Wilkins X-Ray diffraction image of DNA taken by Franklin in 1951 Rosalind Franklin James & The Race toWATSON Discover DNA’s Francis CRICK Structure Awarded Nobel Prize for DNA’s Structure 1953 Compiled data from previous scientists to build a doublehelical model of DNA James Watson Francis Crick The Race to Discover DNA’s Structure was Over • DNA is made up of: – Four nucleotides: Adenine, Thymine, Guanine and Cytosine – These follow the rules of base-pairing: • Adenine bonds with Thymine • Guanine bonds with Cytosine – A sugar-phosphate backbone • DNA is arranged in a double-helix Complimentary Strands • Complementary Sequences: Two strands of nucleotides that stick together because they have “opposite” sequences that form “base pairs”. • (A-T; G-C) Hey, nice sequence! Thanks! You too! DNA Replication • The double helix did explain how DNA copies itself • We will study this process, DNA replication, in more detail DNA Replication is Semi-Conservative Each old strand provides the information to build a new strand. DNA Replication The “parent” molecule has two complementary strands of DNA. Each is base paired by hydrogen bonding with its specific partner: A with T G with C DNA Replication The first step in replication is the separation of the two strands. DNA Replication Each parental strand now serves as a template that determines the order of the bases along a new complementary strand. DNA Replication The nucleotides are connected to form the sugar-phosphate backbones of the new strands. Each “daughter” DNA molecule consists of one parental strand and one new strand. Replication Enzymes • DNA replication involves many enzymes. • DNA Polymerase: Builds new strands of DNA from nucleotides by using old strand sequence KEY IDEAS • DNA base-pairing rules: A=T and G=C because of shape & charge • DNA structure: – Double helix with bases on the INSIDE. Sugar & Phosphate OUTSIDE • DNA replication is semi-conservative Each new molecule made has 1 old and 1 new strand Replication Activity • You will now model the process of DNA replication with your team. • Follow the step-by-step guide and you can’t go wrong.