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General Biology (BIO 10) MWF 11:00 – 11:50 Exam 1 Study Guide Exam Format: 50 (2 pts each) multiple choice questions + 10 extra credit (1 pt each) multiple choice questions. Study Hints: Read through each chapter carefully. Review your notes and fill out any parts that you missed. Make sure to get a copy of someone’s notes for any days you missed. Study vocabulary so that you can recognize words used in context. Make sure that you understand the concepts that we discussed. Chapter 1: What is a kingdom? What 6 kingdoms are recognized in Biology? What are the properties of life? Be able to recognize the various levels of the organization of life. Unifying themes: evolution, flow of energy, cooperation, structure determines function, homeostasis. Inductive vs. deductive reasoning. Review the scientific method, especially controls, hypotheses, theories. Chapter 2: Anatomy & basic properties of an atom Ions & Isotopes C14 dating Oxidation & reduction Different types of bonds (ionic, covalent, hydrogen, peptide) Important characteristics of water pH scale & Buffers Chapter 3: Macromolecules (carbohydrates, proteins, lipids, nucleic acids & their building blocks) Polymers & Monomers Carbohydrates – simple sugars Proteins – amino acids Levels of protein structure Nucleic acids – nucleotides Lipids – fatty acids Glucose polymers glycogen & starch Saturated vs. unsaturated fatty acids Chapter 4: Basic cell & organelle structure – functions of different organelles What is an organelle? Cell Theory Fluid Mosaic model of membrane structure Phospholipids Origin of mitochondria & chloroplasts Prokaryotes vs. Eukaryotes Cilia & flagella Transport across membranes (diffusion [simple & facilitated], osmosis, active transport) Endocytosis & exocytosis Chapter 5: Potential and kinetic energy 1st and 2nd Laws of Thermodynamics Entropy Activation energy Catalysis & enzymes Enzyme function Factors that affect enzyme function How cells regulate Enzyme function ATP structure & function Chapter 6: Photosynthesis know the 2 parts of the light reaction & the Calvin cycle – main purpose of each Where does photosynthesis occur? In which cells? In which organelle? Parts of a chloroplast Reactants & products of photosynthesis (equation) Photons Photosystems I and II Chemiosmosis (know how it works & that it is important in photosynthesis & cellular resp.) Photorespiration C3, C4 and CAM photosynthesis Chapter 7: Reactants & products of cellular respiration (equation) Know the parts of cellular respiration (glycolysis, Krebs cycle, electron transport chain) Where does each occur? Function of cellular respiration When is oxygen necessary? Electron carrier molecules (like NAD+ and FADH+) How many ATP from Glycolysis, Krebs & electron transport chain? How NADH & FADH2? All cells can use glycolysis. Fermentation – in absence of oxygen fermentation resets NADH to NAD+ so glycolysis continues Chapter 8: Cell cycle basics (Interphase (G1, S, G2), Mitosis (PMAT), & Cytokinesis) Mitosis what happens during each phase of mitosis? When is DNA duplicated in mitosis & meiosis? What is a chromosome? Centromere Sister chromatids Homologous Chromosomes Chapter 9: In meiosis, when do homologous chromosomes separate? When do sister chromatids separate in meiosis? Know what happens in meiosis I vs. meiosis II Synapsis & Crossing over (Prophase I) Independent assortment (Metaphase I) Diploid vs. haploid What processes increase genetic diversity? Chapter 10: Heredity Dominant vs. recessive traits Genes & Alleles Homozygous (true-breeding) vs. Heterozygous Genotype vs. phenotype Ratios 3:1 and 1:2:1 Know how to use a Punnett square for one trait (Pp x Pp) Incomplete dominance & Codominance Continuous variation Pleiotropic effects Environmental effects Epistasis What is a testcross for? Mendel’s laws (segregation & independent assortment) ABO blood types (phenotypes A, B, O, AB & genotypes OO, AO, AA, AB, BB, BO) Chromosomal theory of inheritance Pedigrees Chapter 11: Structure of DNA Nucleotides & their structure (phosphate group, sugar, nitrogenous base) Nucleotide bases adenine guanine cytosine & thymine – which pairs with which? Complementary strands of DNA – be able to match a strand to its complement. (ATGGCCA - ?) Basics of DNA replication DNA Polymerase Mutations Chapter 12: Mechanism of gene expression – central dogma mRNA, tRNA Codons & anticodons Function of transcription & translation and where each occurs in the cell RNA polymerase Genetic code (be able to read chart) – universal Regulating gene expression