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Transcript
DNA Replication Questions Where do we get our nucleotides? Approximately how many bases make up the human genome? What is the error rate for duplicating DNA? What contributes to errors being made during replication? What prevents this from becoming permanent damage? Proteins and Enzymes used in Replication 1. Helicase – to unwind the DNA double helix 2. Single strand binding protein – stabilize the unwound parental DNA 3. Primase – inserts a RNA primer to help DNA nucleotides join onto the parent strand [only one needed on the continuous strand while many are seen on the ‘lagging strand’ (5’ – 3’)] 4. DNA polymerase III – synthesize (put together) DNA nucleotides onto the parental strand 5. DNA polymerase I – gets rid of the RNA primer [one on the continuous strand and many on the ‘lagging strand’] 6. DNA ligase – joins the Okazaki fragments to the growing strand