Download Questions on DNA Replication and Enzymes used in DNA replication

DNA Replication Questions
Where do we get our nucleotides?
Approximately how many bases make up the
human genome?
What is the error rate for duplicating DNA?
What contributes to errors being made
during replication?
What prevents this from becoming permanent
damage?
Proteins and Enzymes used in Replication
1. Helicase – to unwind the DNA double helix
2. Single strand binding protein – stabilize
the unwound parental DNA
3. Primase – inserts a RNA primer to help
DNA nucleotides join onto the parent
strand [only one needed on the continuous
strand while many are seen on the ‘lagging
strand’ (5’ – 3’)]
4. DNA polymerase III – synthesize (put
together) DNA nucleotides onto the
parental strand
5. DNA polymerase I – gets rid of the RNA
primer [one on the continuous strand and
many on the ‘lagging strand’]
6. DNA ligase – joins the Okazaki fragments
to the growing strand