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Roll no………………. total no. of pages: 3 1563 BASIC BIOCHEMISTY (2K5) MSC.MLT, 1ST SEM (2096) Time: 3 Hours Max marks: 75 Note: Part-A is compulsory attempt any nine Questions from PART-B. PART-A (2x15=30) (To answer each question in 40-60 words) 1. A. explains briefly the principle of conservation of energy. B. Distinguishes between anabolic and catabolic reactions. C. How is the energy generated during metabolic processes usually stored for later use? D. Which of the following are reducing sugars: (i) Galactose (ii) Mannose (iii) Xylose (iv) Ribose (v) Gluconic acid E. Calculate the isoelectric point of histidine. F. List those amino acids that absorb UV light strongly. G. Distinguish between primary and secondary proteins structures. H. What forces hold protein sub units in a quaternary structure? I. How would you define a glycolipid? Give two examples. J. Draw a structure showing the conformation of cholesterol. K. Write the imino form of cytosine. L. What is a suicide substrate of an enzyme? M. Differentiate between water soluble and fat soluble vitamins. N. What is meant by DNA mutation? O. Define the terms: (i) Transcription (ii) Translation (15x2=30) PART-B 2. Explain briefly the second law of thermodynamics. 3. Why the monosaccharide nits in oligo and polysaccharides are called residues? 4. write the open chain structures for a. D-Gluconic acid and b. D-Gluconic acid 5. What are the important no covalent interactions within proteins? How do weak interactions result in a stable structure? 6. What are the reasons for the marked stability of a helix? 7. Distinguish between a. Chromosome and genome b. Chemical composition of DNA and RNA. 8. Define the terms. a. Sequence complexity b. Sequence complementarity 9. Write the basic difference between the reactions catalyzed by an isomerare? Give example. 10. Write short notes on: 11. (a) Vitamin C deficiency (b) Coenzyme. How are the Okazaki fragments initiated? 12. What is the mechanism of DNA gyrase action? 13. Explain briefly the molecular events in the initiation of Replication in Bacteria. (9*5=45)