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Transcript
Protein Synthesis Practice Name: ____________________Per: _____ Directions: 1. Complete each complementary DNA strand. 2. Transcribe the complementary strand into mRNA 3. Translate the mRNA into tRNA 4. Use Table A to identify the amino acid that corresponds to each anticodon 5. Use Table B to identify the protein coded for by that strand of DNA 6. Identify the kind of mutation that makes each pair of proteins different Table A: Transfer RNA Anticodons alanine arginine asparagine aspartic acid cysteine glutamic acid glutamine glycine histidine isoleucine CGA GCA UUA CUA CGG CGU CGC GCG GCU UCC UCU UUG CUG leucine lysine methionine phenylalanine AAU AAC UUU UUC UAC AAA AAG ACA ACG CUU CUC proline serine GGA GGG GGU GGC AGA AGG AGU AGC UCA GUU CCA GUA UAA threonine tryptophan tyrosine valine UGA UGG UGU UGC ACC AUA AUG CAA CAG CAU CAC GUC CCG CCU CCC GUG UAG UAU GAA GAG GAU Table B: Amino Acid sequences code for these proteins: Glutamic acid + Glutamic Acid + Phenylalanine + Phenylalanine + Tyrosine + Tyrosine + Aspartic Acid + Glycine + Serine + Arginine + Asparagine + Lysine + Serine + Serine + Arginine + Serine + Alanine + Cysteine + Histidine Histadine Proline Proline Cysteine Isoleucine polypeptide for brown eyes polypeptide for blue eyes polypeptide for wavy hair polypeptide for straight hair polypeptide for dimples polypeptide for no dimples 1. This protein used for: _____________________________________ glu asp ser his brown colored eyes 2. glu gly ser his blue eyes (pt mutation changed one letter in 2nd amino acid from gac to ggc) point mutation, substitution This protein used for: _____________________________________ blue eyes # 1 and #2 are different because of a point mutation 3. This protein used for: _____________________________________ tyr asp ala cys dimples 4. tyr lys cys iso no dimples – added an a to get a frame shift This protein used for: _____________________________________ Insertion causing frame shift. Inserted extra A #3 and 4 are different because of a frame shift mutation 5. This protein used for: _____________________________________ phe arg ser pro straight hair 6. This protein used for: _____________________________________ phe ser arg pro wavy hair inversion of 2nd and 3rd anticodons #5 and 6 are different because of an inversion.
