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Transcript
Bozeman DNA Replication Name _____________________ http://www.youtube.com/watch?v=FBmO_rmXxIw Watch the video using the link shown above to learn about DNA replication. Answer the questions below: 1. 2. 3. 4. 5. When in the cell cycle does DNA replication occur? What do prokaryotes use as a method to copy their cells? What are the three theories of DNA replication? How did the Meselson-Stahl experiment prove the semi-conservative theory? In the semiconservative theory, where does the DNA split? What are the two “old” strands used for in the process? 6. Why is DNA said to be “anti-parallel”? How is each side of the DNA labeled? 7. Fill out the chart below, which identifies the key proteins involved in DNA replication. Label each one’s function. ENZYME Helicase FUNCTION Single strand binding proteins DNA polymerase RNA primase DNA ligase 8. To which end of the DNA can DNA polymerase add new nucleotides? 9. How does the process of adding new nucleotides to the leading strand differ from the process of adding nucleotides to the lagging strand? 10. Why is an RNA primer needed in DNA replication? 11. What are Okazaki fragments? 12. How many origins of replication are there in prokaryotes? 13. Why do eukaryotes need several origins of replication moving in both directions?