Download What is the NUTRIENT needed for growth and repair

Scientist who invented PCR and received the
Nobel Prize in 1993
ANNEALING
Process of H bond formation when
complementary base pairs bind
DNA PRIMER
Short single stranded DNA. Enables DNA
polymerase to bind to single-stranded DNA
NUCLEOTIDE
Organic base, sugar and phosphate; the
monomer of DNA
AMPLIFICATION
Exponential increase in the amount of DNA
produced in PCR
RESTRICTION ENDONUCLEASE
Enzyme catalysing hydrolysis reaction,
breaking sugar-phosphate backbone in the
DNA double helix at a specific site
DNA polymerase isolated from bacterium and
used in PCR
Taq POLYMERASE
PCR CYCLE
The sequence of denaturation, annealing and
extension, repeated in PCR
Thermus Aquaticus
Bacterium which lives in high temperatures
such as hot springs; provides enzyme Taq
polymerase
GENE PROBE
Small length of single-stranded DNA used to
locate a specific base sequence on a longer
section of DNA
95oC FOR 60 s
Temperature and time required to separate
DNA strands
DENATURATION
Process of separation of DNA strands
55oC
Temperature required to allow primers to
anneal
dNTP
Deoxyribonucleoside triphosphate
72oC
Temperature required for DNA polymerase to
bind and begin working
THERMOSTABLE
Ability to maintain conformational shape and
remain active at high temperatures
KARY MULLIS