Autophagy_Marc
... - Annotation of big families is easier when done in teams or based on subject. Part can be left to curators with certain expertise. Example: PTHR24073 (Rab proteins family): over 2700 proteins Function in vesicular transport. ...
... - Annotation of big families is easier when done in teams or based on subject. Part can be left to curators with certain expertise. Example: PTHR24073 (Rab proteins family): over 2700 proteins Function in vesicular transport. ...
Proteomic analysis of nipple aspirate fluid from women with early
... the detection of proteins and protein-derived peptides is mass spectrometry (MS) [12-14]. Using surface-enhanced laser desorption ionization time-of-flight (SELDI-TOF) MS, we previously found differences in the phenotypic proteomic profiles of nipple aspirate fluid (NAF) samples from patients with e ...
... the detection of proteins and protein-derived peptides is mass spectrometry (MS) [12-14]. Using surface-enhanced laser desorption ionization time-of-flight (SELDI-TOF) MS, we previously found differences in the phenotypic proteomic profiles of nipple aspirate fluid (NAF) samples from patients with e ...
Developmental Genetics
... cows. In 2003, a cloned mule became the first sterile animal to be so reproduced (Woods et al. 2003). Thus it appears that the nuclei of vertebrate adult somatic cells contain all the genes needed to generate an adult organism. No genes necessary for development have been lost or mutated in the soma ...
... cows. In 2003, a cloned mule became the first sterile animal to be so reproduced (Woods et al. 2003). Thus it appears that the nuclei of vertebrate adult somatic cells contain all the genes needed to generate an adult organism. No genes necessary for development have been lost or mutated in the soma ...
Course Objectives - Suffolk County Community College
... Identify the caloric contributions of proteins, fats, and carbohydrates when they are oxidized. Determine what percentage of total calories in a sample diet is supplied by a given macronutrient from food intake data. Comment on the recommended caloric intake for a moderately active adult and the lim ...
... Identify the caloric contributions of proteins, fats, and carbohydrates when they are oxidized. Determine what percentage of total calories in a sample diet is supplied by a given macronutrient from food intake data. Comment on the recommended caloric intake for a moderately active adult and the lim ...
Green Fluorescent Protein (GFP) Purification Kit
... One of the basic tools of modern biotechnology is DNA splicing, cutting DNA and linking it to other DNA molecules. The basic concept behind DNA splicing is to remove a functional DNA fragment—let’s say a gene—from one organism and combine it with the DNA of another organism in order to make the prot ...
... One of the basic tools of modern biotechnology is DNA splicing, cutting DNA and linking it to other DNA molecules. The basic concept behind DNA splicing is to remove a functional DNA fragment—let’s say a gene—from one organism and combine it with the DNA of another organism in order to make the prot ...
SERK and APOSTART. Candidate Genes for
... nucleotides (accession no. AJ841698). Genomic sequencing of PpSERK1 showed that it is 5,188 nucleotides in length and contains 11 exons and 10 introns with a conserved exon/intron structure (supplemental data) identified in other SERK genes (Nolan et al., 2003). PpSERK2 is 2,246 nucleotides in lengt ...
... nucleotides (accession no. AJ841698). Genomic sequencing of PpSERK1 showed that it is 5,188 nucleotides in length and contains 11 exons and 10 introns with a conserved exon/intron structure (supplemental data) identified in other SERK genes (Nolan et al., 2003). PpSERK2 is 2,246 nucleotides in lengt ...
Haemophilus parasuis Candidate Subunit Vaccine Carol A. Lichtensteiger and Eric R. Vimr
... terminus protein sequence data to identify the neuraminidase gene in genomic libraries for cloning. This protein sequencing requires adequate purification of the neuraminidase protein. We optimized neuraminidase purification to obtain a protein preparation adequate for sequencing. While optimizing, ...
... terminus protein sequence data to identify the neuraminidase gene in genomic libraries for cloning. This protein sequencing requires adequate purification of the neuraminidase protein. We optimized neuraminidase purification to obtain a protein preparation adequate for sequencing. While optimizing, ...
Biogenesis, Structure, and Function of Integral Membrane Proteins
... channel; (iii) they fold into active enzymes through mechanisms that are only vaguely understood; (iv) they mediate processes essential to the life of the cell that include vectorial enzymatic reactions and information transfer; and (v) when they require degradation, cells use mechanistically fascin ...
... channel; (iii) they fold into active enzymes through mechanisms that are only vaguely understood; (iv) they mediate processes essential to the life of the cell that include vectorial enzymatic reactions and information transfer; and (v) when they require degradation, cells use mechanistically fascin ...
3 - Rudner Lab - Harvard University
... is coupled to the completion of landmark morphological events. We refer to this dependence on morphogenesis for gene expression as “morphological coupling.” Three examples of morphological coupling in prokaryotes are reviewed in which the activation of a transcription factor is tied to the assembly ...
... is coupled to the completion of landmark morphological events. We refer to this dependence on morphogenesis for gene expression as “morphological coupling.” Three examples of morphological coupling in prokaryotes are reviewed in which the activation of a transcription factor is tied to the assembly ...
Highly conserved epitope domain in major core protein p24 is
... acid sequence alignment clearly indicates considerable homology not only between HIV-1 and SIVAGM, but also between HIV-1 and FIV (Fig. 3), implying that the region might be important for this protein to exhibit its essential function. In the epitope regions consisting of 15 amino acids, although on ...
... acid sequence alignment clearly indicates considerable homology not only between HIV-1 and SIVAGM, but also between HIV-1 and FIV (Fig. 3), implying that the region might be important for this protein to exhibit its essential function. In the epitope regions consisting of 15 amino acids, although on ...
p66a and p66b of the Mi-2/NuRD complex
... structure (6), binding of several regulatory factors is influenced or inhibited. This has been shown for the DNA binding factors NF-kB (7), E2F (8), CpG binding protein CGBP (9), GABP (10,11), CREB (12), as well as for the insulator factor CTCF (13,14). Although in these cases an inhibition of DNA b ...
... structure (6), binding of several regulatory factors is influenced or inhibited. This has been shown for the DNA binding factors NF-kB (7), E2F (8), CpG binding protein CGBP (9), GABP (10,11), CREB (12), as well as for the insulator factor CTCF (13,14). Although in these cases an inhibition of DNA b ...
Insertional inactivation studies of the csmA and csmC genes of the
... acetylene, and ethylene, can be used to inhibit the synthesis of Bchl d and chlorosomes [26], yet the chlorosome proteins are still present in such cells in large amounts (V. Nguyen and J.G. Ormerod, unpublished results). These observations are consistent with the idea that chlorosomes and/or speci¢ ...
... acetylene, and ethylene, can be used to inhibit the synthesis of Bchl d and chlorosomes [26], yet the chlorosome proteins are still present in such cells in large amounts (V. Nguyen and J.G. Ormerod, unpublished results). These observations are consistent with the idea that chlorosomes and/or speci¢ ...
The cotton MYB108 forms a positive feedback
... Further analysis demonstrated that GhMYB108 interacted with the calmodulin-like protein GhCML11, and the two proteins form a positive feedback loop to enhance the transcription of GhCML11 in a calcium-dependent manner. Verticillium dahliae infection stimulated Ca2+ influx into the cytosol in cotton ...
... Further analysis demonstrated that GhMYB108 interacted with the calmodulin-like protein GhCML11, and the two proteins form a positive feedback loop to enhance the transcription of GhCML11 in a calcium-dependent manner. Verticillium dahliae infection stimulated Ca2+ influx into the cytosol in cotton ...
Monooxygenases in the Butane and Cyclohexane Degradation
... until now. Studies indicated that this enzyme could be related to the soluble butane monooxygenase. The cyclohexane pathway contains a second monooxygenase, type: Baeyer-Villiger monooxygenase (BVO). The sequences of many of these enzymes are published, their sequences are diverse. The enrichment of ...
... until now. Studies indicated that this enzyme could be related to the soluble butane monooxygenase. The cyclohexane pathway contains a second monooxygenase, type: Baeyer-Villiger monooxygenase (BVO). The sequences of many of these enzymes are published, their sequences are diverse. The enrichment of ...
Precursor of human adenovirus core polypeptide Mu targets the
... sequence, expressed N-terminal to the EGFP sequences. On the right, the subcellular distribution of each fusion protein is indicated as No. (nucleolar), Np. (nucleoplasmic) and/or Cyt. (cytoplasmic). (B) Mutants of Mu, formed by substitution of selected arginine codons with alanine codons. Mu protei ...
... sequence, expressed N-terminal to the EGFP sequences. On the right, the subcellular distribution of each fusion protein is indicated as No. (nucleolar), Np. (nucleoplasmic) and/or Cyt. (cytoplasmic). (B) Mutants of Mu, formed by substitution of selected arginine codons with alanine codons. Mu protei ...
Structure and Function of Neurons
... on/within the ribosomes. Peripheral proteins, which are soluble and live in the cytoplasm, are synthesized on free polysomes and transported directly into the dendrites and axons. ...
... on/within the ribosomes. Peripheral proteins, which are soluble and live in the cytoplasm, are synthesized on free polysomes and transported directly into the dendrites and axons. ...
Exploring Prostate Proteins and Antibodies
... Which proteins are expressed in the prostate? To acknowledge Prostate Cancer Awareness Month, we take a closer look at the human prostate specific proteome. We explore the Human Protein Atlas's analysis of the prostate specific transcriptome, and present selected antibodies against proteins specific ...
... Which proteins are expressed in the prostate? To acknowledge Prostate Cancer Awareness Month, we take a closer look at the human prostate specific proteome. We explore the Human Protein Atlas's analysis of the prostate specific transcriptome, and present selected antibodies against proteins specific ...
"Nitrogen Fixation: 1888-2001"
... Nitrogen fixation genes have been identified in a number of cyanobacteria but have been characterised in most detail in Anabaena PCC7120, Anabaena variabilis ATCC 29413 and Synechococcus strain RF-1. Nitrogen-fixing cyanobacteria can be divided into those species in which nitrogen fixation occurs in ...
... Nitrogen fixation genes have been identified in a number of cyanobacteria but have been characterised in most detail in Anabaena PCC7120, Anabaena variabilis ATCC 29413 and Synechococcus strain RF-1. Nitrogen-fixing cyanobacteria can be divided into those species in which nitrogen fixation occurs in ...
pdf-Dokument - Universität Bonn
... which acts on small GTP-binding proteins of the Ras superfamily. GAP proteins have a conserved structure and use similar mechanisms, promoting hydrolysis of GTP to GDP. GAPs include several groups based on their substrate proteins, such as ARF (ADP Ribosylation Factor) GAPs, RAB (RAS-like protein in ...
... which acts on small GTP-binding proteins of the Ras superfamily. GAP proteins have a conserved structure and use similar mechanisms, promoting hydrolysis of GTP to GDP. GAPs include several groups based on their substrate proteins, such as ARF (ADP Ribosylation Factor) GAPs, RAB (RAS-like protein in ...
Essential role of conserved DUF177A protein in plastid 23S rRNA
... S1), whereas genomes of red and green algae typically contain single DUF177 proteins that have varying affinities for the A and B groups and bacterial DUF177 proteins. While this relationship is consistent with the hypothesis that the DUF177A and DUF177B clades arose via duplication of an ancestral ...
... S1), whereas genomes of red and green algae typically contain single DUF177 proteins that have varying affinities for the A and B groups and bacterial DUF177 proteins. While this relationship is consistent with the hypothesis that the DUF177A and DUF177B clades arose via duplication of an ancestral ...
... injected must match the mature “trimmed” mRNA sequence for the gene and the interference could not be elicited by intron sequences. This implies that interference takes place after transcription, probably in the cytoplasm rather than in the cell nucleus (4) The mRNA was revealed to be targeted with ...
Developmentally Controlled Farnesylation
... was labeled strongly in the presence of both PFT and [3H]FPP but not with PGGT-I using either [3H]FPP or [3H]GGPP (Fig. 1A). AtNAP1;1 was also labeled ...
... was labeled strongly in the presence of both PFT and [3H]FPP but not with PGGT-I using either [3H]FPP or [3H]GGPP (Fig. 1A). AtNAP1;1 was also labeled ...
View - Max-Planck
... NKX2.1 gene activity specifies distinct ventral territories within the foregut and forebrain (Lazzaro et al. 1991; Price et al. 1992; Ericson et al. 1995; Shimamura et al. 1995; Kimura et al. 1996; Pera and Kessel 1997). In this respect, NKX2.1 is reminiscent of other vertebrate members of the NK2-f ...
... NKX2.1 gene activity specifies distinct ventral territories within the foregut and forebrain (Lazzaro et al. 1991; Price et al. 1992; Ericson et al. 1995; Shimamura et al. 1995; Kimura et al. 1996; Pera and Kessel 1997). In this respect, NKX2.1 is reminiscent of other vertebrate members of the NK2-f ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.