A cap `n` collar protein isoform contains a selective Hox repressor
... 2B). The predicted 805 amino acid CncB protein thus is distinguished from CncA by a 272 amino acid region that includes His-Pro repeats, Ala-repeats, a Pro-repeat and Val-Gly repeats, but exhibits no extended sequence similarity to other proteins in database searches besides the CNC/b-ZIP domain tha ...
... 2B). The predicted 805 amino acid CncB protein thus is distinguished from CncA by a 272 amino acid region that includes His-Pro repeats, Ala-repeats, a Pro-repeat and Val-Gly repeats, but exhibits no extended sequence similarity to other proteins in database searches besides the CNC/b-ZIP domain tha ...
Mycobacterial virulence and specialized secretion: same story
... However, an intact RD1 region was required to ensure that these proteins were secreted by the bacterium8,11,12. This virulence region was therefore identified as a new specialized secretion system with an unknown purpose. Specialized secretion systems are hallmarks of virulence in Gram-negative bact ...
... However, an intact RD1 region was required to ensure that these proteins were secreted by the bacterium8,11,12. This virulence region was therefore identified as a new specialized secretion system with an unknown purpose. Specialized secretion systems are hallmarks of virulence in Gram-negative bact ...
Question paper - Unit F215 - Control, genomes and
... This question is about the evolution, genetics, behaviour and physiology of cats. Fig. 1.1 (on the insert) shows a Scottish wildcat, Felis sylvestris. Modern domestic cats evolved from a wild ancestor of similar appearance to the Scottish wildcat. Fig. 1.2 (also on the insert) shows a breed of domes ...
... This question is about the evolution, genetics, behaviour and physiology of cats. Fig. 1.1 (on the insert) shows a Scottish wildcat, Felis sylvestris. Modern domestic cats evolved from a wild ancestor of similar appearance to the Scottish wildcat. Fig. 1.2 (also on the insert) shows a breed of domes ...
In vivo characterization of the properties of SUMO1
... component RanGAP [3,4], many other nuclear and extranuclear SUMO substrates have been characterized and numerous putative targets have been suggested by proteomic studies [5,6]. Most SUMOylated proteins identified so far play key roles in genome integrity, nuclear structure and transcription [7,8]. ...
... component RanGAP [3,4], many other nuclear and extranuclear SUMO substrates have been characterized and numerous putative targets have been suggested by proteomic studies [5,6]. Most SUMOylated proteins identified so far play key roles in genome integrity, nuclear structure and transcription [7,8]. ...
Studies of focal adhesion assembly
... Fn3. High-resolution structures of many of these component domains and domain combinations have been available for some time [6]; the main remaining uncertainties are associated with how Fn forms complexes with its various partners. Fn has conformational flexibility that exposes different binding si ...
... Fn3. High-resolution structures of many of these component domains and domain combinations have been available for some time [6]; the main remaining uncertainties are associated with how Fn forms complexes with its various partners. Fn has conformational flexibility that exposes different binding si ...
Bacteriophage EMS9: Preliminary Genomic Description
... listed first, followed by the position (in base pairs) where the ORF occurs, the DNA strand on which the information is encoded and finally, the nature of the predicted protein is listed. Certain of the ORFs listed encode proteins that are predicted to function in a variety of processes important fo ...
... listed first, followed by the position (in base pairs) where the ORF occurs, the DNA strand on which the information is encoded and finally, the nature of the predicted protein is listed. Certain of the ORFs listed encode proteins that are predicted to function in a variety of processes important fo ...
Full-Text PDF
... inbuilt repair and maintenance, which adds complexity. Even the most primitive of needs, the replacement of component proteins, requires a simple genome encoding the proteins and provision of transcription and translational machinery (or simply translational machinery if the genome is manufactured o ...
... inbuilt repair and maintenance, which adds complexity. Even the most primitive of needs, the replacement of component proteins, requires a simple genome encoding the proteins and provision of transcription and translational machinery (or simply translational machinery if the genome is manufactured o ...
View PDF - Faculty Web Sites at the University of Virginia
... Mutations of nod, another Drosophila protein, yield phenotypes that are nearly identical to those of ncd mutants, but are restricted to non-exchange chromosomes [7*]. Although mutants for either nod or ncd are recessive, double heterozygotes have a phenotype similar to that of homozygous nod mutants ...
... Mutations of nod, another Drosophila protein, yield phenotypes that are nearly identical to those of ncd mutants, but are restricted to non-exchange chromosomes [7*]. Although mutants for either nod or ncd are recessive, double heterozygotes have a phenotype similar to that of homozygous nod mutants ...
biochem ch 45 [9-2
... o Large vWF multimers more effective at forming complexes with platelets than are small vWF multimers o vWF deficiency most common cause of inherited bleeding disorders; both platelet adherence and clotting cascade affected because levels of factor VIII low In absence of vWF, factor VIII rapidly c ...
... o Large vWF multimers more effective at forming complexes with platelets than are small vWF multimers o vWF deficiency most common cause of inherited bleeding disorders; both platelet adherence and clotting cascade affected because levels of factor VIII low In absence of vWF, factor VIII rapidly c ...
Plant Molecular Biology
... domain. The transcript can be found in all tested plant organs, but roots and nodules show the highest levels of RH2-1 mRNA accumulation. Promoter-GUS fusion studies demonstrate that the activity of this gene is closely correlated with development of lateral roots (in alfalfa and Arabidopsis) and sy ...
... domain. The transcript can be found in all tested plant organs, but roots and nodules show the highest levels of RH2-1 mRNA accumulation. Promoter-GUS fusion studies demonstrate that the activity of this gene is closely correlated with development of lateral roots (in alfalfa and Arabidopsis) and sy ...
Effects of phosphatidylethanolamine glycation on lipid–protein
... glycation by forming adducts with PE. Moreover, these authors found these complexes in human red blood cells, suggesting that this compound may act as a lipid glycation inhibitor in vivo, and demonstrated that supplementation of the diet of diabetic rats with pyridoxal 5 -phosphate reduces the leve ...
... glycation by forming adducts with PE. Moreover, these authors found these complexes in human red blood cells, suggesting that this compound may act as a lipid glycation inhibitor in vivo, and demonstrated that supplementation of the diet of diabetic rats with pyridoxal 5 -phosphate reduces the leve ...
Protein S-nitrosylation in photosynthetic organisms: A
... 2.1. Major systems of NO production In animal systems the production of nitric oxide (NO) has been extensively characterized over the last decades. Nowadays it is universally accepted that the main pathway goes through nitric oxide synthase (NOS), a ubiquitous enzyme existing in three isoforms: neur ...
... 2.1. Major systems of NO production In animal systems the production of nitric oxide (NO) has been extensively characterized over the last decades. Nowadays it is universally accepted that the main pathway goes through nitric oxide synthase (NOS), a ubiquitous enzyme existing in three isoforms: neur ...
Regulation of microtubule dynamics by microtubule
... tubulin for GTP (Hamel et al., 1983). As MAPs bind to the carboxy terminal region of tubulin subunits (Serrano et al., 1984a,b, 1985), and this domain of the tubulin molecule is also involved in an intramolecular interaction with the GTP-binding site (Padilla et al.. 1993), the association of MAPs w ...
... tubulin for GTP (Hamel et al., 1983). As MAPs bind to the carboxy terminal region of tubulin subunits (Serrano et al., 1984a,b, 1985), and this domain of the tubulin molecule is also involved in an intramolecular interaction with the GTP-binding site (Padilla et al.. 1993), the association of MAPs w ...
4-Biochemical structure of Hemoglobin
... Unable to transport O2 due to abnormal structure: • Carboxy-Hb: CO replaces O2 and binds 200X tighter than O2 (in smokers) ...
... Unable to transport O2 due to abnormal structure: • Carboxy-Hb: CO replaces O2 and binds 200X tighter than O2 (in smokers) ...
Proteomic Approach to Identify Novel
... An Arabidopsis mitochondrial proteome project was started for a comprehensive investigation of mitochondrial functions in plants. Mitochondria were prepared from Arabidopsis stems and leaves or from Arabidopsis suspension cell cultures, and the purity of the generated fractions was tested by the res ...
... An Arabidopsis mitochondrial proteome project was started for a comprehensive investigation of mitochondrial functions in plants. Mitochondria were prepared from Arabidopsis stems and leaves or from Arabidopsis suspension cell cultures, and the purity of the generated fractions was tested by the res ...
Genes for Two Mitochondrial Ribosomal Proteins in
... genes. Numit rps13 from Arabidopsis contains two introns. The genomic sequence of numit rps13 from soybean was determined by polymerase chain reaction amplification and sequencing, revealing two introns in the same positions as those in Arabidopsis. Because the rps13 genes from legumes and Arabidops ...
... genes. Numit rps13 from Arabidopsis contains two introns. The genomic sequence of numit rps13 from soybean was determined by polymerase chain reaction amplification and sequencing, revealing two introns in the same positions as those in Arabidopsis. Because the rps13 genes from legumes and Arabidops ...
Rat maf related genes: specific expression in chondrocytes
... thus interrupting erythroid dierentiation (Sieweke et al., 1996). Maf-family transcription factors heterodimerize not only with bZip proteins, but also with helix ± turn ± helix proteins. Thus, a wide variety of Maf-heterodimers are formed, which in turn could regulate a wide variety of target gene ...
... thus interrupting erythroid dierentiation (Sieweke et al., 1996). Maf-family transcription factors heterodimerize not only with bZip proteins, but also with helix ± turn ± helix proteins. Thus, a wide variety of Maf-heterodimers are formed, which in turn could regulate a wide variety of target gene ...
Analysis of Protein Phosphorylation Using Mass Spectrometry
... enriched proteins are still too complicated for phosphorylation analysis after enzymatic digestion. It can be ameliorated by coupling with a chromatographic enrichment. IMAC or TiO2 has become a common tool for phosphopeptide enrichment. No experimental evidence has shown inconsistencies in binding ...
... enriched proteins are still too complicated for phosphorylation analysis after enzymatic digestion. It can be ameliorated by coupling with a chromatographic enrichment. IMAC or TiO2 has become a common tool for phosphopeptide enrichment. No experimental evidence has shown inconsistencies in binding ...
“molecular” versus “colloidal”: controversies in biology and
... Applying X-ray diffraction studies and calculations, he was instrumental in elucidating the role of weak bonds in macromolecules. In 1936 he suggested that hydrogen and other weak forces determine the three-dimensional structure of proteins and were thus a pre-requisite for their function and biolog ...
... Applying X-ray diffraction studies and calculations, he was instrumental in elucidating the role of weak bonds in macromolecules. In 1936 he suggested that hydrogen and other weak forces determine the three-dimensional structure of proteins and were thus a pre-requisite for their function and biolog ...
PDF
... promise in this pursuit. By comparing the DNA sequence of organisms separated by a range of evolutionary distances, experimenters have been able to identify important features of both entire genomes and individual genes and their protein products (14–18). In this report, we describe a comparative ap ...
... promise in this pursuit. By comparing the DNA sequence of organisms separated by a range of evolutionary distances, experimenters have been able to identify important features of both entire genomes and individual genes and their protein products (14–18). In this report, we describe a comparative ap ...
The UUAG-specific RNA Binding Protein, Heterogeneous Nuclear
... It is also common to find an auxiliary RNA-binding motif present in addition to RBDs within the same molecule. Thus, RBD class RNA binding proteins typically possess several RNA-binding domains as modules. It has not been well studied, however, how these modular domains participate together in bindi ...
... It is also common to find an auxiliary RNA-binding motif present in addition to RBDs within the same molecule. Thus, RBD class RNA binding proteins typically possess several RNA-binding domains as modules. It has not been well studied, however, how these modular domains participate together in bindi ...
Review Structural Insights into Biological Roles of Protein
... heparin, which has been used as a clinical anticoagulant for several decades. Several studies have focused on biochemical characterization of the anticoagulant activity of heparin in terms of its specific interaction with antithrombin III (see below for details). The GAG biosynthetic enzymes have be ...
... heparin, which has been used as a clinical anticoagulant for several decades. Several studies have focused on biochemical characterization of the anticoagulant activity of heparin in terms of its specific interaction with antithrombin III (see below for details). The GAG biosynthetic enzymes have be ...
H 2 O Reabsorption
... ~70% of Filtrate is – Small plasma proteins reabsorbed in PCT – Some urea and uric acid Na+ is actively reabsorbed: First – simple diffusion: Then – 1o active transport: ...
... ~70% of Filtrate is – Small plasma proteins reabsorbed in PCT – Some urea and uric acid Na+ is actively reabsorbed: First – simple diffusion: Then – 1o active transport: ...
Database resources of the National Center for Biotechnology
... These databases include DNA and protein sequences derived from several sources (1,3±6), the NCBI taxonomy, genomes, population sets, gene expression data, gene-oriented sequence clusters in UniGene, sequence-tagged sites in UniSTS, genetic variations in dbSNP, protein structures from the Molecular M ...
... These databases include DNA and protein sequences derived from several sources (1,3±6), the NCBI taxonomy, genomes, population sets, gene expression data, gene-oriented sequence clusters in UniGene, sequence-tagged sites in UniSTS, genetic variations in dbSNP, protein structures from the Molecular M ...
the pdf - University of British Columbia
... ~250kDa, have both been found to inhibit neurite outgrowth in vitro (Schwab & Caroni, 1988; Caroni & Schwab, 1988). As well, in vivo studies have successfully blocked the neurite inhibiting properties of these two proteins and enhanced axonal regeneration after CNS injury (Schnell & Schwab, 1990). ...
... ~250kDa, have both been found to inhibit neurite outgrowth in vitro (Schwab & Caroni, 1988; Caroni & Schwab, 1988). As well, in vivo studies have successfully blocked the neurite inhibiting properties of these two proteins and enhanced axonal regeneration after CNS injury (Schnell & Schwab, 1990). ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.