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A biofilm-forming marine bacterium producing proteins
A biofilm-forming marine bacterium producing proteins

... another cell bound fraction, non soluble in a salt solution (36g/l). None of them is pure. EPS1 consists mainly in a majority of carbohydrates while the two others consist mainly in a majority of proteins. ™Gel electrophoresis analysis showed different EPS proteins composition with molecular weight ...
the pdf - University of British Columbia
the pdf - University of British Columbia

... ~250kDa, have both been found to inhibit neurite outgrowth in vitro (Schwab & Caroni, 1988; Caroni & Schwab, 1988). As well, in vivo studies have successfully blocked the neurite inhibiting properties of these two proteins and enhanced axonal regeneration after CNS injury (Schnell & Schwab, 1990). ...
View the BIO 105 Study Guide
View the BIO 105 Study Guide

... *identify monomers and polymers and how these are important in living things *understand how monomers are used to build polymers *understand the functions of each of the four macromolecules 4. Cell structure and function/differences between prokaryotic and eukaryotic cells *describe the composition ...
Prospects for understanding avirulence gene function Frank F White
Prospects for understanding avirulence gene function Frank F White

... Critical, yet unknown, structural features that are involved in specificity for avirulence and virulence lie within the repeat domains [19,24,52]. Functional nuclear localization signals (NLS) are present in the carboxyl-coding portion of the prototype gene product [53], and are required for the avi ...
#315: Ribosomes: Unlocking the secrets to your cellular protein
#315: Ribosomes: Unlocking the secrets to your cellular protein

... information. It is a carrier of amino acids these are the components that make the proteins. It's called transfer RNA. The RNA that comes through as genetic information is called messenger RNA and then there is ribosomal RNA. In the beginning of ribosomes for at least three decades, people thought t ...
Deletion of Lipoteichoic Acid Synthase Impacts
Deletion of Lipoteichoic Acid Synthase Impacts

... LTA deletion were identified by pairwise comparison of each LTA+ strain (NCK1909 and NCK2030) with each LTA− strain (NCK2025 and NCK2187). Genes meeting the cutoffs for DE in all four comparisons were considered differentially expressed due to absence of LTA. Each pairwise comparison was visualized ...
GST SF in E. coli - Institute for Genomic Biology
GST SF in E. coli - Institute for Genomic Biology

... • Phenomics is difficult with HAD SF members, as many are promiscuous housekeeping phosphatases • An abrupt shift from a relatively poor carbon source to galactonate as sole carbon source causes the YidA KO to display a growth lag – The “abruptness” may be important for quickly building levels of th ...
Protein S-nitrosylation in photosynthetic organisms: A
Protein S-nitrosylation in photosynthetic organisms: A

... 2.1. Major systems of NO production In animal systems the production of nitric oxide (NO) has been extensively characterized over the last decades. Nowadays it is universally accepted that the main pathway goes through nitric oxide synthase (NOS), a ubiquitous enzyme existing in three isoforms: neur ...
Changes in Cell Morphology and the Cellular Localization of Protein
Changes in Cell Morphology and the Cellular Localization of Protein

... could suppress the dis1 phenotype. They named this novel protein dis1-suppressing protein kinase, or dsk1. Upon further characterization, it has been found that Dsk1 phosphorylates serine/arginine residues, and can autophosphorylate both tyrosine and serine-arginine residues. Phosphorylation states ...
Document
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... analyze several proteins at the same time. The proteins can be superimposed in order to deduce structural alignments and compare their active sites or any other relevant parts. Amino acid mutations, H-bonds, angles and distances between atoms are easy to obtain thanks to the intuitive graphic and me ...
vts_6580_8973.
vts_6580_8973.

... "white"; αίμα, "blood") and reflects the finding that lymphocytes are found in abnormally high numbers in the blood of patients presenting with these malignancies. Hematological malignancies are classified according to the cell lineage they originate from. Thus, lymphoblastic or lymphocytic malignan ...
This article appeared in a journal published by Elsevier. The
This article appeared in a journal published by Elsevier. The

... reducing accumulation of antiviral silencing-associated viral siRNAs produced largely by the host RNA-dependent RNA polymerase 1 which is salicylic acid-inducible [12–14]. Interestingly, CMV 2b can also significantly reduce transgene de novo DNA methylation, inferring its potential role in interferin ...
CETT CDG handout for clinicians AKT 4-30-10 17-39-08
CETT CDG handout for clinicians AKT 4-30-10 17-39-08

... biochemically, genetic testing is required to determine the type and subtype of CDG. Because of the wide variety and overlap of symptoms seen in affected individuals, it is very difficult to identify which CDG gene may be responsible for the symptoms in any given patient. While single gene testing i ...
RbcL | Rubisco large subunit, form I and form II (50 µl) product
RbcL | Rubisco large subunit, form I and form II (50 µl) product

... Kolesinski et al. (2014). Rubisco Accumulation Factor 1 from Thermosynechococcus elongatus participates in the final stages of ribulose-1,5-bisphosphate carboxylase/oxygenase assembly in Escherichia coli cells and in vitro. FEBS J. 2014 Jul 12. doi: 10.1111/febs.12928 Pandey and Pandey-Rai (2014). M ...
1 mec-15 Encodes an F-box Protein Required for Touch
1 mec-15 Encodes an F-box Protein Required for Touch

... In this paper we describe the molecular and phenotypic characterization of mec15, which defines an additional ubiquitin ligase pathway that regulates multiple aspects of TRN development. The TRNs in mec-15 mutants do not develop or function properly; they have reduced chemical synapses, enlarged, ab ...
Peer-reviewed Article PDF
Peer-reviewed Article PDF

... of the amino acid phenylalanine and reduction of tyrosine. High levels of phenylalanine and low of tyrosine characterize phenylketonuria (PKU; OMIM 261600) disease whereas moderately increased levels of phenylalanine and/or reduced or normal levels of tyrosine are usually measured in hyperphenylalan ...
Signatures of nitrogen limitation in the elemental composition of the
Signatures of nitrogen limitation in the elemental composition of the

... carbon : nitrogen ratios between 1.5 and 9 in amino acids and between 1 and 2.5 in nucleotides). Thus, nutrient limitation may in principle influence the molecular composition of genes and proteins by favouring monomer usage biases that conserve the limiting elements. Indeed, a number of recent stud ...
7.06 Cell Biology EXAM #3
7.06 Cell Biology EXAM #3

... And be sure to put your NAME ON EACH PAGE in case they become separated! There are SEVEN pages including this cover sheet. ...
figure 1 - Proceedings of the Royal Society B
figure 1 - Proceedings of the Royal Society B

... carbon : nitrogen ratios between 1.5 and 9 in amino acids and between 1 and 2.5 in nucleotides). Thus, nutrient limitation may in principle influence the molecular composition of genes and proteins by favouring monomer usage biases that conserve the limiting elements. Indeed, a number of recent stud ...
BL414 Genetics Spring 2006 Test 1 Key February 8, 2006
BL414 Genetics Spring 2006 Test 1 Key February 8, 2006

... named Gregor Mendel figured out that genes are made up of doublehelical DNA. False 2) (2.5pts.) T or F: A single gene may have effects on seemingly unrelated traits in an organism such as pigmentation and amino acid metabolism. True 3) (2.5pts.) T or F: Mid-twentieth century experiments involving th ...
Sno/Ski Proto-Oncogene Family - The Newfeld Lab
Sno/Ski Proto-Oncogene Family - The Newfeld Lab

... mouse developed spontaneous lymphomas, suggesting that Sno could function as a tumor suppressor gene. Two other Sno knockout mice, generated with a similar strategy, did not develop lymphoma but rather displayed developmental defects in T-cell proliferation. Studies utilizing small interfer­ ing RNA ...
Identification and characterization of genetic variants in the
Identification and characterization of genetic variants in the

... investigations and analysis of such rarely existed bovine families are the essential pre-requisite: To identify more LZM+ animals in the general dairy cattle population through lys-mic genotyping. To search out more new genetic variants for LZM+ within mLys gene. A trait associated (viz., SCC, Leuko ...
Noureddine Loukili  Studies on UEV, a new regulator of polyubiquitination:
Noureddine Loukili Studies on UEV, a new regulator of polyubiquitination:

... adopts a stable compact globular conformation with four strands of β-sheet and a single α-helix. Its name comes from the fact that it is abundant in all eukaryotes, and extraordinarily well conserved in creatures as divers as yeast and human. The three carboxy-terminal residues, -Arg-Gly-Gly, are fl ...
Hulmi, Finland: Enhancing Muscle Mass and Function
Hulmi, Finland: Enhancing Muscle Mass and Function

... • DNA does not incorporate with the host genome with AAV-viruses • Minor side effects – AAV is not currently known to cause disease – The virus causes a very mild immune response ...
Protein–DNA Interactions: Amino Acid Conservation and the Effects
Protein–DNA Interactions: Amino Acid Conservation and the Effects

... have variable levels of conservation depending on the family classification. In non-specific families, base-contacting residues are well conserved and interactions are always found in the minor groove where there is little discrimination between base types. In highly specific families, base-contacti ...
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Protein moonlighting



Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.
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