Immunoprecipitation protocol
... the serum Ig is being removed effectively. If serum has not been sufficiently removed, bands will be present at 50 and 25 kDa for heavy and light chains; its presence may contribute to a weak immunoprecipitation. Consider either decreasing the amount of serum or increasing the amount of beads incuba ...
... the serum Ig is being removed effectively. If serum has not been sufficiently removed, bands will be present at 50 and 25 kDa for heavy and light chains; its presence may contribute to a weak immunoprecipitation. Consider either decreasing the amount of serum or increasing the amount of beads incuba ...
A Serine/Proline-Rich Protein Is Fused To HRX in t(4
... observed upstream of each initiating ATG, indicating that the open reading frames could not extend further in the 5’ direction. Data base searches indicated that the predicted proteins lacked significant similarity to previously reported proteins. Motif searches showed a guanosine triphosphate (GTP) ...
... observed upstream of each initiating ATG, indicating that the open reading frames could not extend further in the 5’ direction. Data base searches indicated that the predicted proteins lacked significant similarity to previously reported proteins. Motif searches showed a guanosine triphosphate (GTP) ...
Regulation of Heat Shock Response in Yeast and - E
... 1 Differentiation lineage-dependent regulation of human heat shock transcription factor 2 in K562 erythroleukemia cells (I) ..................................... 28 1.1 HSF2 is activated and upregulated in K562 cells specifically within erythroid differentiation ..................................... ...
... 1 Differentiation lineage-dependent regulation of human heat shock transcription factor 2 in K562 erythroleukemia cells (I) ..................................... 28 1.1 HSF2 is activated and upregulated in K562 cells specifically within erythroid differentiation ..................................... ...
25/05
... 4. Now do ex. 9.2 by adding another subroutine that takes: (1) a protein accession, (2) a protein length and (3) a reference to such a hash, and returns 0 if the accession is not found, 1 if the length is identical to the one in the hash, and 2 otherwise. 5.* Now add a third input file and check if ...
... 4. Now do ex. 9.2 by adding another subroutine that takes: (1) a protein accession, (2) a protein length and (3) a reference to such a hash, and returns 0 if the accession is not found, 1 if the length is identical to the one in the hash, and 2 otherwise. 5.* Now add a third input file and check if ...
Depth-stratified functional and taxonomic niche
... evolve under different selective pressures than their host versions and have recombined back into the host (Sullivan et al., 2006). Cyanophage AMGs can also evolve to the point that they perform modified function. For example, when discovered, viral pebS was most similar to a cyanobacterial pebA gen ...
... evolve under different selective pressures than their host versions and have recombined back into the host (Sullivan et al., 2006). Cyanophage AMGs can also evolve to the point that they perform modified function. For example, when discovered, viral pebS was most similar to a cyanobacterial pebA gen ...
Secreted Hepatitis B Surface Antigen Polypeptides Are Derived from
... chains have shifted down to a molecular mass of •17,000 D (labeled F). This shift down in molecular mass is identical to the change previously observed (5) when proteolysis was performed on the products generated in cell-free translation/ translocation experiments. A control experiment in which vesi ...
... chains have shifted down to a molecular mass of •17,000 D (labeled F). This shift down in molecular mass is identical to the change previously observed (5) when proteolysis was performed on the products generated in cell-free translation/ translocation experiments. A control experiment in which vesi ...
Protein Dynamics in the Plant Extracellular Space
... sensing changes in the cell wall structure and transduce the message to the cytoplasm [33]. 1.5. A Proteomics Approach to the ECS The increasing knowledge on the ECS has reinforced the view of the fundamental role played by this compartment for cell function and plant metabolism. Information on the ...
... sensing changes in the cell wall structure and transduce the message to the cytoplasm [33]. 1.5. A Proteomics Approach to the ECS The increasing knowledge on the ECS has reinforced the view of the fundamental role played by this compartment for cell function and plant metabolism. Information on the ...
MB207_10 - MB207Jan2010
... ligand (signaling molecule e.g. hormones, growth factors, neurotransmitters). - Binding of ligand is followed by specific chemical events in the inner surface of the membrane that often lead to changes in gene expression and cell function. 5) Membrane provide mechanism for cell-to-cell contact, adhe ...
... ligand (signaling molecule e.g. hormones, growth factors, neurotransmitters). - Binding of ligand is followed by specific chemical events in the inner surface of the membrane that often lead to changes in gene expression and cell function. 5) Membrane provide mechanism for cell-to-cell contact, adhe ...
Proti-Ace Kit - Hampton Research
... 4. Set the crystallization experiment using the protease:sample mixture. Optimization of In Situ Proteolysis for Crystallization a. Vary the protease:sample ratio. Typical protease:sample ratios are 1:100, 1:1,000 and 1:10,000. b. Alter the incubation time. Typical incubation times are between 0 and ...
... 4. Set the crystallization experiment using the protease:sample mixture. Optimization of In Situ Proteolysis for Crystallization a. Vary the protease:sample ratio. Typical protease:sample ratios are 1:100, 1:1,000 and 1:10,000. b. Alter the incubation time. Typical incubation times are between 0 and ...
Genes can be switched on and off by the protein CTCF
... I injected DNA into mouse embryos, using a special reporting system to see if CTCF was active or not. Dependent upon whether the DNA contained a binding site for CTCF so that CTCF could bind and function, or contained no such binding site, different signals were expected. However, I could not observ ...
... I injected DNA into mouse embryos, using a special reporting system to see if CTCF was active or not. Dependent upon whether the DNA contained a binding site for CTCF so that CTCF could bind and function, or contained no such binding site, different signals were expected. However, I could not observ ...
Whole body and tissue protein synthesis in cattle
... The deposition of protein in the body depends upon the difference in the amounts of protein synthesized and degraded per unit time, It is an energy-dependent process influenced by nutritional, environmental, hormonal and genetic treatments. In recent years considerable effort has been directed towar ...
... The deposition of protein in the body depends upon the difference in the amounts of protein synthesized and degraded per unit time, It is an energy-dependent process influenced by nutritional, environmental, hormonal and genetic treatments. In recent years considerable effort has been directed towar ...
Cytochrome P450 - Spektrum der Wissenschaft
... Metabolism of eicosapentaenoic acid by CYP4A and CYP2C enzymes CYP4A and CYP2C enzymes hydroxylate and/or epoxygenate arachidonic acid (AA; 20:4 n-6) to metabolites involved in the regulation of vascular tone and kidney function. Eicosapentaenoic acid (EPA; 20:5 n-3) is structurally closely related ...
... Metabolism of eicosapentaenoic acid by CYP4A and CYP2C enzymes CYP4A and CYP2C enzymes hydroxylate and/or epoxygenate arachidonic acid (AA; 20:4 n-6) to metabolites involved in the regulation of vascular tone and kidney function. Eicosapentaenoic acid (EPA; 20:5 n-3) is structurally closely related ...
Datasheet - Santa Cruz Biotechnology, Inc.
... The p53 gene is a widely studied anti-oncogene, or tumor suppressor gene. The p53 gene product can act as a negative regulator of cell growth in response to DNA damage. Mutations and allelic loss of the p53 gene have been associated with malignant transformation in a wide variety of human tumors. p5 ...
... The p53 gene is a widely studied anti-oncogene, or tumor suppressor gene. The p53 gene product can act as a negative regulator of cell growth in response to DNA damage. Mutations and allelic loss of the p53 gene have been associated with malignant transformation in a wide variety of human tumors. p5 ...
A Modern History Theory of Functions - Peter Godfrey
... dispositions.5 If so, we should remain with the simpler analysis that allows any survivalenhancing power, however subversive, to qualify as a function. Although some may favor this more heartless approach I will adopt a third, intermediate position. Consider first another counterintuitive consequenc ...
... dispositions.5 If so, we should remain with the simpler analysis that allows any survivalenhancing power, however subversive, to qualify as a function. Although some may favor this more heartless approach I will adopt a third, intermediate position. Consider first another counterintuitive consequenc ...
Natural Genetic Variation Modifies Gene Expression
... B,F,G,J in S1 Figure). Furthermore, FUS3 protein per mRNA was significantly higher in S288c for most time-points (Fig 2G, 3G,L, and Fig B,F,G,J in S1 Figure), all together indicating another likely case of variation in protein synthesis and/or protein decay rates between strains. Although GPH1 had d ...
... B,F,G,J in S1 Figure). Furthermore, FUS3 protein per mRNA was significantly higher in S288c for most time-points (Fig 2G, 3G,L, and Fig B,F,G,J in S1 Figure), all together indicating another likely case of variation in protein synthesis and/or protein decay rates between strains. Although GPH1 had d ...
Identification of Pexl3p, a Peroxisomal Membrane Receptor for the
... have hitherto not been identified. In addition to the identification of the PTS1 and PTS2 recognition factors, the genetic approaches led to the discovery of 16 additional P E X genes, formerly known as PAS, PER, PEB, or P A Y genes (see accompanying letter in this issue), whose gene products, desig ...
... have hitherto not been identified. In addition to the identification of the PTS1 and PTS2 recognition factors, the genetic approaches led to the discovery of 16 additional P E X genes, formerly known as PAS, PER, PEB, or P A Y genes (see accompanying letter in this issue), whose gene products, desig ...
Chapters 5, 7, 11, 17 ppt
... 19 have the same general structure: central (a) carbon, an amino group, carboxylate group, hydrogen atom, and an R group (proline is the exception) At pH 7, the carboxyl group is in its conjugate base form (-COO-) while the amino group is its conjugate acid form (NH3+); therefore, it is amphoteric ...
... 19 have the same general structure: central (a) carbon, an amino group, carboxylate group, hydrogen atom, and an R group (proline is the exception) At pH 7, the carboxyl group is in its conjugate base form (-COO-) while the amino group is its conjugate acid form (NH3+); therefore, it is amphoteric ...
NMR Analysis of Protein
... overlay spectra in presence/absence of ligand changes in peak position indicate binding identity of peaks that change identifies binding site on protein surface if a defined residue cluster is not observed non-specific binding if a majority of the peaks incur changes detrimental interacti ...
... overlay spectra in presence/absence of ligand changes in peak position indicate binding identity of peaks that change identifies binding site on protein surface if a defined residue cluster is not observed non-specific binding if a majority of the peaks incur changes detrimental interacti ...
Functional and Evolutionary Analysis of the CASPARIAN STRIP
... GFP variants, expressed them under the control of the AtCASP1 promoter, and compared their localization with a wild-type AtCASP1-mCherry (Vermeer et al., 2014). Besides the transmembrane domains, conservation in CASPLs is found in the second extracellular loop (EL2). EL1 is poorly conserved among CA ...
... GFP variants, expressed them under the control of the AtCASP1 promoter, and compared their localization with a wild-type AtCASP1-mCherry (Vermeer et al., 2014). Besides the transmembrane domains, conservation in CASPLs is found in the second extracellular loop (EL2). EL1 is poorly conserved among CA ...
Assembly and disassembly of the influenza C matrix protein layer on
... Insight into the inner workings of viruses is an important piece of knowledge in humanity’s expanding collection of knowledge which can potentially be used to treat or prevent diseases. Influenza C is known to cause an infection with cold-like symptoms with possible complications in young children. ...
... Insight into the inner workings of viruses is an important piece of knowledge in humanity’s expanding collection of knowledge which can potentially be used to treat or prevent diseases. Influenza C is known to cause an infection with cold-like symptoms with possible complications in young children. ...
Not Every Disulfide Lasts Forever: Disulfide Bond
... Cellular compartments differ dramatically in their redox potentials. This translates directly into variations in the extent of disulfide bond formation within proteins, depending on their cellular localization. It has long been assumed that proteins that are present in the reducing environment of th ...
... Cellular compartments differ dramatically in their redox potentials. This translates directly into variations in the extent of disulfide bond formation within proteins, depending on their cellular localization. It has long been assumed that proteins that are present in the reducing environment of th ...
Allosteric regulation and catalysis emerge via a common
... large number of conformations that may differ from each other only slightly, and this collection of conformers is considered the native-state ensemble. This view relies on the idea that there are regions of varying stability in a given protein30. For a catalytic cycle, there are multiple sequential ...
... large number of conformations that may differ from each other only slightly, and this collection of conformers is considered the native-state ensemble. This view relies on the idea that there are regions of varying stability in a given protein30. For a catalytic cycle, there are multiple sequential ...
Microbial enzyme secretion
... Corynebacterium glutamicum is a very important industrial strain for amino acid production. This study investigated establishing this microbe for protein secretion. Aspergillus protein secretion http://bmcsystbiol.biomedcentral.com/articles/10.1186/ ...
... Corynebacterium glutamicum is a very important industrial strain for amino acid production. This study investigated establishing this microbe for protein secretion. Aspergillus protein secretion http://bmcsystbiol.biomedcentral.com/articles/10.1186/ ...
Early Development
... What is the connection between β-catenin and glycogen synthase kinase – 3 (GSK3)? ...
... What is the connection between β-catenin and glycogen synthase kinase – 3 (GSK3)? ...
Regulation of secondary metabolism in streptomycetes
... secondary metabolic gene clusters that they regulate (those for virginiamycin M and virginiamycin S [27]). Similarly, although initially recognized by their role in regulating Act and Red production, the corresponding genes of S. coelicolor, scbA and scbR, are adjacent to, and directly regulate, a ...
... secondary metabolic gene clusters that they regulate (those for virginiamycin M and virginiamycin S [27]). Similarly, although initially recognized by their role in regulating Act and Red production, the corresponding genes of S. coelicolor, scbA and scbR, are adjacent to, and directly regulate, a ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.