Modification-specific proteomics: Strategies for characterization of
... for identifying candidate substrate proteins for certain types of PTM, such as tyrosine phosphorylation. However, the small size of the structural motifs of other common PTMs (for example, protein methylation and acetylation) makes it difficult to generate pan-specific antibodies, which recognize PT ...
... for identifying candidate substrate proteins for certain types of PTM, such as tyrosine phosphorylation. However, the small size of the structural motifs of other common PTMs (for example, protein methylation and acetylation) makes it difficult to generate pan-specific antibodies, which recognize PT ...
Evolutionary Gain of Function for the ER Membrane
... Sec62 and Sec63 are expected to play a role in protein biogenesis in the ER. We characterized interactions between these two proteins as well as the putative interaction of Sec62 with ribosomes. These data provide further evidence for evolutionary conservation of Sec62/Sec63 interaction. In addition ...
... Sec62 and Sec63 are expected to play a role in protein biogenesis in the ER. We characterized interactions between these two proteins as well as the putative interaction of Sec62 with ribosomes. These data provide further evidence for evolutionary conservation of Sec62/Sec63 interaction. In addition ...
Ascorbate peroxidaserelated (APxR) is a new
... characterized a new class of putative heme peroxidases, called ascorbate peroxidase-related (APx-R), which arose specifically in the lineage of plants. • The APx-R protein is structurally related to the ascorbate peroxidases, although the active site contains many conserved substitutions. Unlike all ...
... characterized a new class of putative heme peroxidases, called ascorbate peroxidase-related (APx-R), which arose specifically in the lineage of plants. • The APx-R protein is structurally related to the ascorbate peroxidases, although the active site contains many conserved substitutions. Unlike all ...
Lecture 7 POWERPOINT here
... Different AA’s are linked together via PEPTIDE bonds The individual amino acids within a protein are known as RESIDUES The smallest known P’ is just nine residues long - oxytocin The largest is over 25,000 residues - the structural protein titin ...
... Different AA’s are linked together via PEPTIDE bonds The individual amino acids within a protein are known as RESIDUES The smallest known P’ is just nine residues long - oxytocin The largest is over 25,000 residues - the structural protein titin ...
1 - UvA-DARE - University of Amsterdam
... Condorelli paper. Since then a consensus has been reached and the protein is now called GFAPδ (Condorelli et al., 1999; Middeldorp and Hol, 2011; Nielsen et al., 2002; Thomsen et al., 2013). Blechingberg and colleagues studied alternative splicing with a GFAP minigene construct and showed that the e ...
... Condorelli paper. Since then a consensus has been reached and the protein is now called GFAPδ (Condorelli et al., 1999; Middeldorp and Hol, 2011; Nielsen et al., 2002; Thomsen et al., 2013). Blechingberg and colleagues studied alternative splicing with a GFAP minigene construct and showed that the e ...
Cofradionation of HeLa Cell Replication Proteins With Ors
... useful in the biochemical analysis of the proteins involved in the replication process. Fractionation of human cell extracts has identified several proteins that are necessary for complete in vitro SV40 DNA replication, which are believed to participate in the same process in the cell. Reconstitutio ...
... useful in the biochemical analysis of the proteins involved in the replication process. Fractionation of human cell extracts has identified several proteins that are necessary for complete in vitro SV40 DNA replication, which are believed to participate in the same process in the cell. Reconstitutio ...
2007-10_GO-resources_jblake
... Following the term ID and name is a number in parentheses. This is the total number of genes manually annotated to this term and its children. Electronic annotations (evidence code IEA) are not shown for two reasons: there are large numbers of these annotations, and they are deemed lower quality as ...
... Following the term ID and name is a number in parentheses. This is the total number of genes manually annotated to this term and its children. Electronic annotations (evidence code IEA) are not shown for two reasons: there are large numbers of these annotations, and they are deemed lower quality as ...
The Nhal antiporter of Saccharomyces cerevisiae mediates sodium
... encoded by the ENAl gene allelic to PMR2 (Haro et al., 1991; Wieland et al., 1995). In most Sacch. cereuisiae strains, ENAl is the first unit of a tandem array of four or five genes, depending on the strain (Garciadeblas et al., 1993;Wieland et al., 1995).ENA2, ENA3 and ENA4 are expressed constituti ...
... encoded by the ENAl gene allelic to PMR2 (Haro et al., 1991; Wieland et al., 1995). In most Sacch. cereuisiae strains, ENAl is the first unit of a tandem array of four or five genes, depending on the strain (Garciadeblas et al., 1993;Wieland et al., 1995).ENA2, ENA3 and ENA4 are expressed constituti ...
Profibrillin conversion by proprotein convertases
... Fibroblasts heterozygous for this mutation converted only onehalf of secreted profibrillin to fibrillin. In order to further characterize the cleavage site and to confirm that the furin/PACE family is responsible for processing profibrillin, we designed a construct encoding a mini-profibrillin prote ...
... Fibroblasts heterozygous for this mutation converted only onehalf of secreted profibrillin to fibrillin. In order to further characterize the cleavage site and to confirm that the furin/PACE family is responsible for processing profibrillin, we designed a construct encoding a mini-profibrillin prote ...
Molecular Clocks
... • Deamidation also has a role in cataracts, The rate of this reaction is preset by the sequence and structure of the peptide or protein and associated peptides or proteins as genetically specified in the DNA. The rate may also be modified by changes in protein structure and solvent conditions in-viv ...
... • Deamidation also has a role in cataracts, The rate of this reaction is preset by the sequence and structure of the peptide or protein and associated peptides or proteins as genetically specified in the DNA. The rate may also be modified by changes in protein structure and solvent conditions in-viv ...
BMC Genomics Daphnia pulex by complete genome sequencing Seanna J McTaggart*
... branch lengths separating some gene pairs in clade II are indicative of either recent duplication or concerted evolution. None of the GNBP clade II proteins have a CR domain, but all of them, except for Dappu-GNBP1, have an active GLU domain. GNBP clade III consists of proteins exclusively from crus ...
... branch lengths separating some gene pairs in clade II are indicative of either recent duplication or concerted evolution. None of the GNBP clade II proteins have a CR domain, but all of them, except for Dappu-GNBP1, have an active GLU domain. GNBP clade III consists of proteins exclusively from crus ...
Expressway™ Cell-Free E. coli Expression System
... Successful use of the Expressway™ Cell-Free E. coli Expression System to synthesize recombinant protein requires the addition of a DNA template containing the gene of interest placed within the proper context of transcription and translation regulatory elements including a bacteriophage T7 RNA polym ...
... Successful use of the Expressway™ Cell-Free E. coli Expression System to synthesize recombinant protein requires the addition of a DNA template containing the gene of interest placed within the proper context of transcription and translation regulatory elements including a bacteriophage T7 RNA polym ...
Biological membranes - Essays in Biochemistry
... through the membrane. Phospholipids can diffuse relatively quickly in the leaflet of the bilayer in which they are located. A phospholipid can travel around the perimeter of a red blood cell in around 12 s, or move the length of a bacterial cell within 1 s. Phospholipids can also spin around on thei ...
... through the membrane. Phospholipids can diffuse relatively quickly in the leaflet of the bilayer in which they are located. A phospholipid can travel around the perimeter of a red blood cell in around 12 s, or move the length of a bacterial cell within 1 s. Phospholipids can also spin around on thei ...
Vacuolar protein sorting mechanisms in plants
... most of the Sar/Arf1 GTPases are well conserved. The Arf1 family is necessary for COPI vesicle and CCV formation, whereas the Sar1 family is mainly involved in COPII vesicle formation [59,60]. After formation, the vesicle is released and travels towards the target compartment assisted by actin filam ...
... most of the Sar/Arf1 GTPases are well conserved. The Arf1 family is necessary for COPI vesicle and CCV formation, whereas the Sar1 family is mainly involved in COPII vesicle formation [59,60]. After formation, the vesicle is released and travels towards the target compartment assisted by actin filam ...
Edgetic perturbation models of human inherited disorders, Mol Syst
... & 2009 EMBO and Macmillan Publishers Limited All rights reserved 1744-4292/09 ...
... & 2009 EMBO and Macmillan Publishers Limited All rights reserved 1744-4292/09 ...
PowerPoint Template
... Hormone-receptor systems and the corresponding signaling chains display great variability and flexibility (Fig. 3.5), which is expressed by the following observations: For a given hormone, different receptors can exist on the same or on different cells. Thus, the same hormone can trigger very di ...
... Hormone-receptor systems and the corresponding signaling chains display great variability and flexibility (Fig. 3.5), which is expressed by the following observations: For a given hormone, different receptors can exist on the same or on different cells. Thus, the same hormone can trigger very di ...
hemoglobin - MBBS Students Club
... important. They have an asymmetric distribution. e.g. AP, AP, AP, AP- Type I AP, AP, PA, AP- Type III ...
... important. They have an asymmetric distribution. e.g. AP, AP, AP, AP- Type I AP, AP, PA, AP- Type III ...
Interaction between Cell Wall and Plasma Membrane via RGD Motif
... The fact that integrin- and vitronectin-like proteins existed in plasma membranes and cell walls, respectively, suggests that an interaction occurs between cell wall protein(s) and plasma membrane protein(s) mediated by RGD-motif. To evaluate such a hypothesis, a far-western blotting analysis was pe ...
... The fact that integrin- and vitronectin-like proteins existed in plasma membranes and cell walls, respectively, suggests that an interaction occurs between cell wall protein(s) and plasma membrane protein(s) mediated by RGD-motif. To evaluate such a hypothesis, a far-western blotting analysis was pe ...
UvA-DARE (Digital Academic Repository) Mitochondria in neutrophil
... Mcl-1 in neutrophils seems to be inhibition of Bax translocation 5, but this thesis has demonstrated that the role of Bfl-1 in the apoptotic process is far from obvious. The function of Bax itself is also not directly clear. This protein is a main contributor to the formation of the PTP. However, it ...
... Mcl-1 in neutrophils seems to be inhibition of Bax translocation 5, but this thesis has demonstrated that the role of Bfl-1 in the apoptotic process is far from obvious. The function of Bax itself is also not directly clear. This protein is a main contributor to the formation of the PTP. However, it ...
Interaction of Sesbania Mosaic Virus Movement Protein
... poly protein 2a, and ORF 2b for the RNA dependent RNA polymerase (RdRp) that is translated by a frame shift mechanism to yield poly protein 2ab. The 39- proximal ORF codes for the CP which is expressed via a sub genomic RNA [16]. The domain arrangement in poly protein 2a and 2ab was recently shown t ...
... poly protein 2a, and ORF 2b for the RNA dependent RNA polymerase (RdRp) that is translated by a frame shift mechanism to yield poly protein 2ab. The 39- proximal ORF codes for the CP which is expressed via a sub genomic RNA [16]. The domain arrangement in poly protein 2a and 2ab was recently shown t ...
DNA sequence of the control region of phage D108: the N
... for the repressor gene, ner gene, and the 5' part of the A^ gene (which codes for transposase) are found in the sequence. The genetic organization of this region of phage D108 is quite similar to that of phage Mu in spite of considerable divergence, both in the nucleotide sequence and in the amino a ...
... for the repressor gene, ner gene, and the 5' part of the A^ gene (which codes for transposase) are found in the sequence. The genetic organization of this region of phage D108 is quite similar to that of phage Mu in spite of considerable divergence, both in the nucleotide sequence and in the amino a ...
Membrane Transport - Austin Publishing Group
... pumps, just to list a few. Further, transport proteins may be classified by the substance that they transport, whether they are always open or gated (open only when stimulated), and most importantly, whether a source of free energy is required for operation. ...
... pumps, just to list a few. Further, transport proteins may be classified by the substance that they transport, whether they are always open or gated (open only when stimulated), and most importantly, whether a source of free energy is required for operation. ...
Contribution of molecular chaperones to protein folding in the
... The process by which a linear polypeptide attains its unique, functionally active, three-dimensional structure from the astronomical number of possible conformations has perplexed researchers for many years [1]. Most of our knowledge concerning the folding and assembly of proteins has come from in v ...
... The process by which a linear polypeptide attains its unique, functionally active, three-dimensional structure from the astronomical number of possible conformations has perplexed researchers for many years [1]. Most of our knowledge concerning the folding and assembly of proteins has come from in v ...
Plant Cell Vacuoles
... to the members of the fructan family, which are not transported through the tonoplast, some members of the raffinose family are taken up by an H 1 -antiport mechanism in plants accumulating these sugars. Amino acid concentrations are often lower in the vacuole than in the cytosol. A transporter modula ...
... to the members of the fructan family, which are not transported through the tonoplast, some members of the raffinose family are taken up by an H 1 -antiport mechanism in plants accumulating these sugars. Amino acid concentrations are often lower in the vacuole than in the cytosol. A transporter modula ...
In vitro phosphorylation of the movement protein of tomato mosaic
... as heparin, suramin and quercetin, which are known to be effective for casein kinase II (CK II). The phosphorylation level was not changed by other types of inhibitor. In addition, as shown for animal and plant CK II, [γ-32P]GTP was efficiently used as a phosphoryl donor. Phosphorylation was not aff ...
... as heparin, suramin and quercetin, which are known to be effective for casein kinase II (CK II). The phosphorylation level was not changed by other types of inhibitor. In addition, as shown for animal and plant CK II, [γ-32P]GTP was efficiently used as a phosphoryl donor. Phosphorylation was not aff ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.