Engineering Tyrosine-Based Electron Flow Pathways in Proteins
... radical location on the basis of EPR hyperfine structure. Mutation to tyrosine decreases the lipid peroxidase activity of this myoglobin in the presence of low concentrations of reductant, and the possibility of decreasing the intrinsic toxicity of hemoglobin by introduction of these pathways is disc ...
... radical location on the basis of EPR hyperfine structure. Mutation to tyrosine decreases the lipid peroxidase activity of this myoglobin in the presence of low concentrations of reductant, and the possibility of decreasing the intrinsic toxicity of hemoglobin by introduction of these pathways is disc ...
Proceedings of the National Academy of Sciences
... All living organisms require zinc for continued growth and development. The numerous functions include both structural and catalytic roles in a number of enzymes (1–3). However, in excess zinc is toxic, so both eukaryotes and prokaryotes have developed mechanisms to prevent overaccumulation of zinc, ...
... All living organisms require zinc for continued growth and development. The numerous functions include both structural and catalytic roles in a number of enzymes (1–3). However, in excess zinc is toxic, so both eukaryotes and prokaryotes have developed mechanisms to prevent overaccumulation of zinc, ...
Roles for Regulator of G Protein Signaling Proteins in Synaptic
... Hollinger and Hepler, 2002; Willars, 2006). RGS proteins are a structurally diverse family of signaling proteins with many identified signaling partners distinct from Ga and GPCRs. In this regard, considerable evidence shows that many RGS proteins have cell signaling roles in addition to their share ...
... Hollinger and Hepler, 2002; Willars, 2006). RGS proteins are a structurally diverse family of signaling proteins with many identified signaling partners distinct from Ga and GPCRs. In this regard, considerable evidence shows that many RGS proteins have cell signaling roles in addition to their share ...
Characterization of a Novel Peripheral Nervous System Myelin
... also provide support for the hypothesis that MAG is involved in axon-Schwann cell interactions (Owens et al., 1990) . Protein zero (PO), MBP, and MAG are the major protein components of PNS myelin (reviewed in Morell et al ., 1989; Lemke, 1988) . P0, a transmembrane glycoprotein which, like MAG, bel ...
... also provide support for the hypothesis that MAG is involved in axon-Schwann cell interactions (Owens et al., 1990) . Protein zero (PO), MBP, and MAG are the major protein components of PNS myelin (reviewed in Morell et al ., 1989; Lemke, 1988) . P0, a transmembrane glycoprotein which, like MAG, bel ...
Figure S1. Interactions between AS1 and CO proteins that contains
... Figure S3. Location of the T-DNA insertion in the as1-21 mutant. (a) The position of T-DNA insertion in AS1 gene. We determined the precise position of TDNA insertion in as1-21. T-DNA was inserted in the 1st intron of AS1 gene (131 bases downstream from the transcription start site of AS1). +1 repre ...
... Figure S3. Location of the T-DNA insertion in the as1-21 mutant. (a) The position of T-DNA insertion in AS1 gene. We determined the precise position of TDNA insertion in as1-21. T-DNA was inserted in the 1st intron of AS1 gene (131 bases downstream from the transcription start site of AS1). +1 repre ...
Report of attending the international CILIA 2016 in Amsterdam
... organelles in mammals, the rebirth of cilia has been driven by the discovery that cilia dysfunction was associated to severe developmental diseases. Now, this research field is very active and many aspects of cilia functions in cell physiology and development are in progress and a growing number of ...
... organelles in mammals, the rebirth of cilia has been driven by the discovery that cilia dysfunction was associated to severe developmental diseases. Now, this research field is very active and many aspects of cilia functions in cell physiology and development are in progress and a growing number of ...
Host cell protein analysis by mass spectrometry and its application
... individual proteins down to single digit ppm (ng/mg) ...
... individual proteins down to single digit ppm (ng/mg) ...
Histidine protein kinases: key signal transducers outside the animal
... HPKs also have phosphatase activity which dephosphorylates the response regulator and opposes kinase function [3,13] (for details see the Mechanism section); phosphatase activity is mediated by the dimerization domain in these HPKs. The catalytic domain of HPKs has clear sequence and structural homo ...
... HPKs also have phosphatase activity which dephosphorylates the response regulator and opposes kinase function [3,13] (for details see the Mechanism section); phosphatase activity is mediated by the dimerization domain in these HPKs. The catalytic domain of HPKs has clear sequence and structural homo ...
The Drosophila pipsqueak gene encodes a nuclear BTB
... cells and somatically derived follicle cells throughout oogenesis and that it is required prior to stage one of oogenesis. PsqA contains a BTB (POZ) domain at its amino terminus; additionally, we have identified an evolutionarily conserved motif of unknown function present four times in tandem at th ...
... cells and somatically derived follicle cells throughout oogenesis and that it is required prior to stage one of oogenesis. PsqA contains a BTB (POZ) domain at its amino terminus; additionally, we have identified an evolutionarily conserved motif of unknown function present four times in tandem at th ...
Viral protein targeting to the cortical endoplasmic reticulum is
... Potato virus X (PVX). GFP-TGBp3 of PVX, like BaMV, was tar geted to the cortical ER tubules as labeled by Rtn1-mCherry in yeast (Fig. 4 C). Furthermore, the putative sorting signal of PVX TGBp3 can functionally replace the one in BaMV in targeting the hybrid protein to the cortical ER tubules (Fig. ...
... Potato virus X (PVX). GFP-TGBp3 of PVX, like BaMV, was tar geted to the cortical ER tubules as labeled by Rtn1-mCherry in yeast (Fig. 4 C). Furthermore, the putative sorting signal of PVX TGBp3 can functionally replace the one in BaMV in targeting the hybrid protein to the cortical ER tubules (Fig. ...
Jacob Corn
... infection even further. First, while initially unfolded protein fragments similar in sequence to the beta-sheet rich portions of PrPSc exhibit spontaneous self-assembly into beta-sheet structures (Baskakov et al., 2000), full-length PrPSc produced in vitro is not sufficient to cause prion infection ...
... infection even further. First, while initially unfolded protein fragments similar in sequence to the beta-sheet rich portions of PrPSc exhibit spontaneous self-assembly into beta-sheet structures (Baskakov et al., 2000), full-length PrPSc produced in vitro is not sufficient to cause prion infection ...
Gene Section SHC4 (SHC (Src homology 2 domain containing) family, member 4)
... Location: 15q21.1 Note: The mammalian Src homology and collagen (SHC) gene family comprises four distinct loci that encode proteins sharing a unique structural organization. SHC proteins function as phosphotyrosine-binding docking molecule mediating the signaling transduction of cell-membrane recept ...
... Location: 15q21.1 Note: The mammalian Src homology and collagen (SHC) gene family comprises four distinct loci that encode proteins sharing a unique structural organization. SHC proteins function as phosphotyrosine-binding docking molecule mediating the signaling transduction of cell-membrane recept ...
Full Text - The International Journal of Developmental Biology
... M,P-R). These results are in line with data by others demonstrating CRIP1 mRNA expression in fetal rat and human hearts (Tsui et al., 1994). Crip1 transcripts were also detected in the tubules of the developing pronephros (Fig. 5U) and lung (Fig 5V). Not much is known about crip1 expression during e ...
... M,P-R). These results are in line with data by others demonstrating CRIP1 mRNA expression in fetal rat and human hearts (Tsui et al., 1994). Crip1 transcripts were also detected in the tubules of the developing pronephros (Fig. 5U) and lung (Fig 5V). Not much is known about crip1 expression during e ...
Interplay of pH and Binding of Multivalent Metal Ions: Charge
... cause overcharging of the surface, attraction between likecharged surfaces, and charge inversion of colloidal particles.30−35 A complete model for the protein charge in any system has to include both local and nonlocal contributions, although many studies focus on only one approach.36,37 The driving ...
... cause overcharging of the surface, attraction between likecharged surfaces, and charge inversion of colloidal particles.30−35 A complete model for the protein charge in any system has to include both local and nonlocal contributions, although many studies focus on only one approach.36,37 The driving ...
Transport of Storage Proteins to Protein Storage Vacuoles Is
... the electron-dense cores of the PAC vesicles and then leave the endoplasmic reticulum. Immunocytochemical analysis also showed that complex glycans are associated with the peripheral region of PAC vesicles but not the electron-dense cores, indicating that Golgi-derived glycoproteins are incorporated ...
... the electron-dense cores of the PAC vesicles and then leave the endoplasmic reticulum. Immunocytochemical analysis also showed that complex glycans are associated with the peripheral region of PAC vesicles but not the electron-dense cores, indicating that Golgi-derived glycoproteins are incorporated ...
involvement of protein tyrosine phosphorylation of human sperm in
... stimulating factor-1 (CSF-1) (30), interferon-γ (IFN-γ ) (35), tumor necrosis factor-α (TNF-α) (34), epidermal growth factor (EGF) (37), thymosin α1 (Tα1) (9), thymosin ß4 (Tß4) (9); and several unidentified factors (29). Some of these cytokines are also present in cervical mucus of women (38, 39), ...
... stimulating factor-1 (CSF-1) (30), interferon-γ (IFN-γ ) (35), tumor necrosis factor-α (TNF-α) (34), epidermal growth factor (EGF) (37), thymosin α1 (Tα1) (9), thymosin ß4 (Tß4) (9); and several unidentified factors (29). Some of these cytokines are also present in cervical mucus of women (38, 39), ...
Arginine-glycine-aspartic acid motif incorporated within turnip yellow
... A tripeptide motif used by Integrins as an attachment point. RGD motifs are found within viral proteins and facilitate cellular adhesion. Integrin (protein receptor) Integrin is a ligand used by some cells and viruses for adhesion and cell signaling. Stem cells up-regulate Integrin productio ...
... A tripeptide motif used by Integrins as an attachment point. RGD motifs are found within viral proteins and facilitate cellular adhesion. Integrin (protein receptor) Integrin is a ligand used by some cells and viruses for adhesion and cell signaling. Stem cells up-regulate Integrin productio ...
Identification of eight novel coagulation factor XIII subunit A mutations
... rarely, by F13B gene defects (5% of cases). The F13A gene, coding for the FXIII A protein subunit, occupies chromosomal position 6p24-25 and comprises 15 exons encoding a 731 amino acid protein.1 Homozygous mutations in this gene usually result in severe FXIII deficiency (OMIM: +134570, +134580), wh ...
... rarely, by F13B gene defects (5% of cases). The F13A gene, coding for the FXIII A protein subunit, occupies chromosomal position 6p24-25 and comprises 15 exons encoding a 731 amino acid protein.1 Homozygous mutations in this gene usually result in severe FXIII deficiency (OMIM: +134570, +134580), wh ...
Essential Bioinformatics and Biocomputing (LSM2104
... • Questions and strategies for dealing with biomedical problems • SARS as an example – SARS genome – How to figure out the function of SARS genes? – Use of info of SARS genes in drug design ...
... • Questions and strategies for dealing with biomedical problems • SARS as an example – SARS genome – How to figure out the function of SARS genes? – Use of info of SARS genes in drug design ...
Biochemical Origins of Alzheimer`s Disease With Treatment
... the likelihood for developing Alzheimer’s disease. The gene encoding the amyloid precursor protein is located on chromosome 21; missense mutations arising in this gene that have been shown to confer an increased risk to AD. Missense mutations in the APP gene increase the overall amount of Athrough ...
... the likelihood for developing Alzheimer’s disease. The gene encoding the amyloid precursor protein is located on chromosome 21; missense mutations arising in this gene that have been shown to confer an increased risk to AD. Missense mutations in the APP gene increase the overall amount of Athrough ...
Review Structural glycobiology: A game of snakes and ladders
... Computational docking methods have evolved to help overcome difficulties in obtaining experimental 3D structures of multiple ligands to a common receptor. Docking strategies can utilize experimental or modeled protein structures to aid in the discovery new lead compounds for drug design. In the fiel ...
... Computational docking methods have evolved to help overcome difficulties in obtaining experimental 3D structures of multiple ligands to a common receptor. Docking strategies can utilize experimental or modeled protein structures to aid in the discovery new lead compounds for drug design. In the fiel ...
The tubby-like proteins - Journal of Cell Science
... TUB and performed X-ray crystallographic analyses. The electron-density map obtained was interpreted as a unique protein structure, a 12-stranded β-barrel conformation filled with a central hydrophobic core that traverses the entire barrel. Two prominent features of the folded protein are a large gr ...
... TUB and performed X-ray crystallographic analyses. The electron-density map obtained was interpreted as a unique protein structure, a 12-stranded β-barrel conformation filled with a central hydrophobic core that traverses the entire barrel. Two prominent features of the folded protein are a large gr ...
General - Bioinformatics Research Group at SRI International
... X = total number of reactions in P Y = enzymes catalyzing number of reactions for which there is evidence in O Z = number of Y reactions that are used in other pathways in O ...
... X = total number of reactions in P Y = enzymes catalyzing number of reactions for which there is evidence in O Z = number of Y reactions that are used in other pathways in O ...
Subsystem: Succinate dehydrogenase
... Subunits containing bound FAD and iron-sulfur centers constitute a peripheral portion of complex II, which can function as a water-soluble succinate dehydrogenase upon release from membranes. The reverse reaction (reduction of fumarate) functions as an electron sink in anaerobic respiration. Two sma ...
... Subunits containing bound FAD and iron-sulfur centers constitute a peripheral portion of complex II, which can function as a water-soluble succinate dehydrogenase upon release from membranes. The reverse reaction (reduction of fumarate) functions as an electron sink in anaerobic respiration. Two sma ...
pGLO GFP purification manual 4006099
... under UV light. After determining that the pGLO plasmid DNA was not responsible for the fluorescence under the UV light, you concluded that it was not the plasmid DNA that was fluorescing in response to the ultraviolet light within the cells. This then led to the next hypothesis that if it is not th ...
... under UV light. After determining that the pGLO plasmid DNA was not responsible for the fluorescence under the UV light, you concluded that it was not the plasmid DNA that was fluorescing in response to the ultraviolet light within the cells. This then led to the next hypothesis that if it is not th ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.