Somatic cancer mutations in the MLL1 histone methyltransferase
... to different partner proteins such as AF4 and AF9 generating oncoproteins, which further leads to deregulated expression of the HoxA9 and Meis1 genes. MLL proteins contain a catalytically active SET [Su (var)3-9, enhancer-of-zeste and trithorax] domain (Cheng et al., 2005; Dillon et al., 2005). In t ...
... to different partner proteins such as AF4 and AF9 generating oncoproteins, which further leads to deregulated expression of the HoxA9 and Meis1 genes. MLL proteins contain a catalytically active SET [Su (var)3-9, enhancer-of-zeste and trithorax] domain (Cheng et al., 2005; Dillon et al., 2005). In t ...
splice sites at the termini generating a novel intron from a dSpm
... for excision. The dSpm element of the original state is an internal deletion derivative of the autonomous En/Spm element but has retained the highly structured ends that have been inferred as substrate sites for transposition (for review, see Gierl et al., 1989). These regions consist of the 13 bp t ...
... for excision. The dSpm element of the original state is an internal deletion derivative of the autonomous En/Spm element but has retained the highly structured ends that have been inferred as substrate sites for transposition (for review, see Gierl et al., 1989). These regions consist of the 13 bp t ...
Stitching proteins into membranes, not sew simple
... bound to SRP is disrupted or at least exhibits some plasticity (to allow hairpin formation) during the insertion process. An alternative variation on the classical model is that the pre-formed TM helix, somehow positions itself correctly in the translocon without helix disruption. As mentioned previ ...
... bound to SRP is disrupted or at least exhibits some plasticity (to allow hairpin formation) during the insertion process. An alternative variation on the classical model is that the pre-formed TM helix, somehow positions itself correctly in the translocon without helix disruption. As mentioned previ ...
Western blot Fast purification Comparative performance
... There are articles comparing various techniques such as isolating small quantities of protein from complex samples; comparing differences in optical density analysis between spectrophotometric instruments; and presenting different methods of protein quantification. We are excited to introduce the my ...
... There are articles comparing various techniques such as isolating small quantities of protein from complex samples; comparing differences in optical density analysis between spectrophotometric instruments; and presenting different methods of protein quantification. We are excited to introduce the my ...
Pole Region-Dependent Repression of the Drosophila Gap Gene
... embryos, the expression domain of Kr in wild-type embryos is relatively small. This suggests that it is Kfdependent activity rather than Kr itself that is responsible for the large gap seen later in amorphic Kr mutant embryos (Jackie et al., 1986). Meinhardt’s theoretical model on Drosophila segment ...
... embryos, the expression domain of Kr in wild-type embryos is relatively small. This suggests that it is Kfdependent activity rather than Kr itself that is responsible for the large gap seen later in amorphic Kr mutant embryos (Jackie et al., 1986). Meinhardt’s theoretical model on Drosophila segment ...
Recruitment of Drosophila Polycomb Group proteins to
... homologous pairing of the transgenes and not simply on the total transgene copy number. PSA also depends on trxG proteins, since it was affected in an AbGAm line tested in a mutant background for the two trxG genes trithorax (trx) or brahma (brm). Conversely, ...
... homologous pairing of the transgenes and not simply on the total transgene copy number. PSA also depends on trxG proteins, since it was affected in an AbGAm line tested in a mutant background for the two trxG genes trithorax (trx) or brahma (brm). Conversely, ...
chordin (H-300): sc-28964 - Santa Cruz Biotechnology
... 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000). chordin (H-300) is also recommended for detection of c ...
... 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000). chordin (H-300) is also recommended for detection of c ...
The lin-41 RBCC Gene Acts in the C. elegans Heterochronic
... supports the prediction that this mutation truncates essential regions from LIN-41. The lin-41(ma104) allele has a Tc1 transposon inserted at amino acid 713. Five other lin-41 mutations cause substitutions in the C-terminal conserved region of the protein (Figure 3 legend, Figure 4). lin-41(mg187) r ...
... supports the prediction that this mutation truncates essential regions from LIN-41. The lin-41(ma104) allele has a Tc1 transposon inserted at amino acid 713. Five other lin-41 mutations cause substitutions in the C-terminal conserved region of the protein (Figure 3 legend, Figure 4). lin-41(mg187) r ...
NUCLEAR PROTEIN KINASE ACTIVITIES DURING THE CELL
... There is extensive experimental evidence that suggests a role for components of the non-histone chromosomal proteins in the regulation of eukaryotic gene expression [1--10]. Many of the non-histone chromosomal proteins are phosphoproteins and modifications in their phosphate metabolism have been ass ...
... There is extensive experimental evidence that suggests a role for components of the non-histone chromosomal proteins in the regulation of eukaryotic gene expression [1--10]. Many of the non-histone chromosomal proteins are phosphoproteins and modifications in their phosphate metabolism have been ass ...
Beyond Antibodies
... DI-Leu16-IL-2 [de-immunized and humanized anti-CD20 monoclonal antibody Leu16 fused to human cytokine interleukin-2 (IL2)] F16–IL2 [Recombinant fusion protein composed of the antibody fragment scFv(F16) (specific to the alternatively spliced domain A1 of tenascin-C) and of human interleukin-2 (I ...
... DI-Leu16-IL-2 [de-immunized and humanized anti-CD20 monoclonal antibody Leu16 fused to human cytokine interleukin-2 (IL2)] F16–IL2 [Recombinant fusion protein composed of the antibody fragment scFv(F16) (specific to the alternatively spliced domain A1 of tenascin-C) and of human interleukin-2 (I ...
WAVE/Scars in platelets
... against 1 of the 3 isoforms of WAVE recognizes a single band in platelet lysates. The relative molecular weights of plateletassociated WAVE1, WAVE2, and WAVE3 were approximately 80, 85, and 70 kDa, respectively. However, in most experiments, WAVE1 and WAVE2 migrated very closely in the gel. It was r ...
... against 1 of the 3 isoforms of WAVE recognizes a single band in platelet lysates. The relative molecular weights of plateletassociated WAVE1, WAVE2, and WAVE3 were approximately 80, 85, and 70 kDa, respectively. However, in most experiments, WAVE1 and WAVE2 migrated very closely in the gel. It was r ...
Heterotrimeric G proteins in C. elegans
... chemosensory neurons. Consistent with their expression patterns, the conserved C. elegans α subunits, GSA-1, GOA-1 and EGL-30 are involved in diverse and fundamental aspects of development and behavior. GOA-1 acts redundantly with GPA-16 in positioning of the mitotic spindle in early embryos. EGL-30 ...
... chemosensory neurons. Consistent with their expression patterns, the conserved C. elegans α subunits, GSA-1, GOA-1 and EGL-30 are involved in diverse and fundamental aspects of development and behavior. GOA-1 acts redundantly with GPA-16 in positioning of the mitotic spindle in early embryos. EGL-30 ...
Marshall Nirenberg - Nobel Lecture
... reported that DNAase inhibited in vitro amino acid incorporation into protein. I had also observed this phenomenon and was greatly interested in it because the results strongly suggested that the cell-free synthesis of protein was dependent, ultimately, upon DNA templates. Heinrich Matthaei then joi ...
... reported that DNAase inhibited in vitro amino acid incorporation into protein. I had also observed this phenomenon and was greatly interested in it because the results strongly suggested that the cell-free synthesis of protein was dependent, ultimately, upon DNA templates. Heinrich Matthaei then joi ...
Cell Division Studies of Escherichia Coli: Expression and Protein
... master. UMI films th e text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer. The quality of th is reproduction is dependent upon the quality of the copy submitted. Broken or indistin ...
... master. UMI films th e text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer. The quality of th is reproduction is dependent upon the quality of the copy submitted. Broken or indistin ...
Proteolytic Enzymes from Extremely Halophilic Bacteria
... Bacteria of the genus Halobacterium are widely distributed in brines of high salt concentration. They are thus conspicuous in solar evaporation ponds of salt works, where they often become the dominating type of organisms when the salt concentration rises above 25 %. The extremely halophilic bacteri ...
... Bacteria of the genus Halobacterium are widely distributed in brines of high salt concentration. They are thus conspicuous in solar evaporation ponds of salt works, where they often become the dominating type of organisms when the salt concentration rises above 25 %. The extremely halophilic bacteri ...
Phosphorylation-Dependent Targeting of Tetrahymena HP1 to
... histone H3K9-methylated chromatin, which is absent from Tetrahymena macronuclei, where Hhp1 resides (25). Instead, macronuclear chromatin contains trimethylated histone H3K27, a mark that is enriched in the highly condensed, repressive “chromatin body” structures within the macronucleus, where Hhp1 ...
... histone H3K9-methylated chromatin, which is absent from Tetrahymena macronuclei, where Hhp1 resides (25). Instead, macronuclear chromatin contains trimethylated histone H3K27, a mark that is enriched in the highly condensed, repressive “chromatin body” structures within the macronucleus, where Hhp1 ...
REVIEW - The Journal of Cell Biology
... reviews of the suborganellar localization of proteins and membrane protein topography have appeared recently (2--4, 35, 58, 60,131). Finally, we describe in greater detail the properties of mitochondrial and chloroplast outer membranes because of their presumptive roles in protein transport. ...
... reviews of the suborganellar localization of proteins and membrane protein topography have appeared recently (2--4, 35, 58, 60,131). Finally, we describe in greater detail the properties of mitochondrial and chloroplast outer membranes because of their presumptive roles in protein transport. ...
Linking folding and binding
... sequences [1,21]. Indeed, it is frequently possible to identify these folding motifs, which have been termed molecular recognition elements or MoRFs, by bioinformatic analysis of the protein sequence [22]. These recognition motifs can fold into helix, b-strand, or form irregular structure on bindin ...
... sequences [1,21]. Indeed, it is frequently possible to identify these folding motifs, which have been termed molecular recognition elements or MoRFs, by bioinformatic analysis of the protein sequence [22]. These recognition motifs can fold into helix, b-strand, or form irregular structure on bindin ...
Senescence-Associated Vacuoles, a Specific Lytic Compartment for
... membrane bound vesicles detected in the cytosol of leaf cells through transmission electron microscopy [33]. Their numbers increased during senescence, and immunolocalization experiments showed that they contained chloroplast stromal proteins, such as Rubisco and glutamine synthetase II, but not thy ...
... membrane bound vesicles detected in the cytosol of leaf cells through transmission electron microscopy [33]. Their numbers increased during senescence, and immunolocalization experiments showed that they contained chloroplast stromal proteins, such as Rubisco and glutamine synthetase II, but not thy ...
MICROSTRUCTURE OF PROTEIN BODIES IN MARAMA BEAN SPECIES
... Yeboah, 1998). Marama and soya had similar fibre contents. However, the total carbohydrate and ash contents were different among these legumes. The carbohydrates contents of marama from this study are lower than the values previously reported by Amarteifio and Moholo, (1998). Changes in chemical com ...
... Yeboah, 1998). Marama and soya had similar fibre contents. However, the total carbohydrate and ash contents were different among these legumes. The carbohydrates contents of marama from this study are lower than the values previously reported by Amarteifio and Moholo, (1998). Changes in chemical com ...
A mitochondrial specific stress response in mammalian cells
... Fig. 2. Transcriptional activation of genes by unfolded proteins in mitochondria. (A) Northern blot analysis of total RNA isolated from COS-7 cells transfected with vector OTC or OTC-D constructs (left panel). The blot was hybridized sequentially with radiolabelled Cpn60, Cpn10, ClpP, BiP, mtHsp70, ...
... Fig. 2. Transcriptional activation of genes by unfolded proteins in mitochondria. (A) Northern blot analysis of total RNA isolated from COS-7 cells transfected with vector OTC or OTC-D constructs (left panel). The blot was hybridized sequentially with radiolabelled Cpn60, Cpn10, ClpP, BiP, mtHsp70, ...
Role of bacteriophage SPP1 tail spike protein gp21 on host cell
... theoretical masses of ~110 kDa and ~123.5 kDa respectively. The mass of gp21 takes into account the new gp21 amino acid sequence numbering used from hereafter that starts at the leucine residue that precedes the aminoterminus sequence SKNIXI determined experimentally for the gp21 present in SPP1 par ...
... theoretical masses of ~110 kDa and ~123.5 kDa respectively. The mass of gp21 takes into account the new gp21 amino acid sequence numbering used from hereafter that starts at the leucine residue that precedes the aminoterminus sequence SKNIXI determined experimentally for the gp21 present in SPP1 par ...
Methods for imaging and detecting modification of proteins
... Products of lipid peroxidation are generated in a wide range of pathologies associated with oxidative stress and inflammation. Many oxidized lipids contain reactive functional groups that can modify proteins, change their structure and function, and affect cell signaling. However, intracellular local ...
... Products of lipid peroxidation are generated in a wide range of pathologies associated with oxidative stress and inflammation. Many oxidized lipids contain reactive functional groups that can modify proteins, change their structure and function, and affect cell signaling. However, intracellular local ...
The Escherichia coli trmE (mnmE) gene, involved in tRNA
... motif (G-2), which is highly conserved within each GTPase family but not between different families, is not involved in GTP binding but is thought to play a role in interaction with the effector molecule. A threonine residue is the only invariant of G-2 between families. The last few years have witn ...
... motif (G-2), which is highly conserved within each GTPase family but not between different families, is not involved in GTP binding but is thought to play a role in interaction with the effector molecule. A threonine residue is the only invariant of G-2 between families. The last few years have witn ...
Mercury, Cadmium, and Arsenite Enhance Heat Shock Protein
... Fig. 1. A: Dose-dependent changes in de novo stress protein synthesis induced in chick embryos exposed to metals. Representative SDSPAGE (12.5% minigels) profiles of 35S-methionine-labeled chick embryonic proteins 2 h after exposure to 0 (Con), 3, 10, 30, or 100 nmol/ embryo of arsenite (As), cadmiu ...
... Fig. 1. A: Dose-dependent changes in de novo stress protein synthesis induced in chick embryos exposed to metals. Representative SDSPAGE (12.5% minigels) profiles of 35S-methionine-labeled chick embryonic proteins 2 h after exposure to 0 (Con), 3, 10, 30, or 100 nmol/ embryo of arsenite (As), cadmiu ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.