Analyses of the E3 ubiquitin-ligase target adapter
... Genes encoding BTB proteins have been identified in a wide range of eukaryotic organisms (including fungi, protists, animals, and plants) but the BTB gene families in different groups show great variability in size, complexity, and composition2. In land plant genomes thus far studied, BTB gene famil ...
... Genes encoding BTB proteins have been identified in a wide range of eukaryotic organisms (including fungi, protists, animals, and plants) but the BTB gene families in different groups show great variability in size, complexity, and composition2. In land plant genomes thus far studied, BTB gene famil ...
Alignment
... alignments by hand if the structure is available. • These alignments can then serve as a benchmark to train gap parameters so that the alignment program produces correct alignments. ...
... alignments by hand if the structure is available. • These alignments can then serve as a benchmark to train gap parameters so that the alignment program produces correct alignments. ...
N-Methylated Amino Acids
... and NG’ (c). Monomethylation at the δ-amino group (d) has also been described. ...
... and NG’ (c). Monomethylation at the δ-amino group (d) has also been described. ...
Full-Text PDF
... catalytically-competent orientation. The crystal structure of MTG revealed that this triad is not conserved; rather, it was proposed that MTG uses a cysteine protease-like mechanism in which Asp255 plays the role of the histidine residue in factor XIII-like TGases [15]. Of the two, MTG is more robus ...
... catalytically-competent orientation. The crystal structure of MTG revealed that this triad is not conserved; rather, it was proposed that MTG uses a cysteine protease-like mechanism in which Asp255 plays the role of the histidine residue in factor XIII-like TGases [15]. Of the two, MTG is more robus ...
1. SVM example: Computational Biology Assume a fixed species
... Applications: classification of proteins into functional vs. structural classes, cellular localization of proteins, and knowledge of protein interactions. Derive kernels (equivalently, appropriate feature maps!) by starting with choices of feature spaces J . ...
... Applications: classification of proteins into functional vs. structural classes, cellular localization of proteins, and knowledge of protein interactions. Derive kernels (equivalently, appropriate feature maps!) by starting with choices of feature spaces J . ...
PDF + SI - GenScript
... in our various preparations of the CMG complex that the helicase activity is ATP-dependent (Fig. 3E Center) and saturates with 1 mM nucleotide (Fig. 3E Right). Two substrates (Fig. 4A Left) were used to test the directionality of the CMG complex tracking movement on DNA. As indicated by the displace ...
... in our various preparations of the CMG complex that the helicase activity is ATP-dependent (Fig. 3E Center) and saturates with 1 mM nucleotide (Fig. 3E Right). Two substrates (Fig. 4A Left) were used to test the directionality of the CMG complex tracking movement on DNA. As indicated by the displace ...
Arbonne-Protein-Shake-Label-Avery
... Arbonne Protein Shake (Chocolate – Vanilla) Add contents to 9 oz. of cold water or almond milk and shake vigorously (add ice and use a blender for a thicker shake). ...
... Arbonne Protein Shake (Chocolate – Vanilla) Add contents to 9 oz. of cold water or almond milk and shake vigorously (add ice and use a blender for a thicker shake). ...
Molecular Genetics of Autosomal-Dominant Demyelinating Charcot
... (Valentijn et al., 1992). This was and still is the most important advance in the understanding of the molecular genetics of CMT as the chromosome 17 duplication remains the major cause of CMT accounting for about 70% of all cases of CMT1 (Nelis et al., 1996). Shortly after the chromosome 17 duplica ...
... (Valentijn et al., 1992). This was and still is the most important advance in the understanding of the molecular genetics of CMT as the chromosome 17 duplication remains the major cause of CMT accounting for about 70% of all cases of CMT1 (Nelis et al., 1996). Shortly after the chromosome 17 duplica ...
PDF - Blood Journal
... and lysophosphatidic acid (LPA) induce Ca⫹⫹-dependent processes.2,3 The rise of cytosolic Ca⫹⫹ in erythrocytes triggers a sequence of biochemical and morphologic changes that finally result in the release of hemoglobin-containing exovesicles. Two sorts of vesicles, differing in size, have been descr ...
... and lysophosphatidic acid (LPA) induce Ca⫹⫹-dependent processes.2,3 The rise of cytosolic Ca⫹⫹ in erythrocytes triggers a sequence of biochemical and morphologic changes that finally result in the release of hemoglobin-containing exovesicles. Two sorts of vesicles, differing in size, have been descr ...
Review Process - The EMBO Journal
... interactions among other insulator DNA-binding proteins. Its own binding to chromatin seems to be in some cases dependent on other insulator proteins and in other cases independent. In this work, the authors have asked if CP190 associates with proteins that might explain its chromatin binding behavi ...
... interactions among other insulator DNA-binding proteins. Its own binding to chromatin seems to be in some cases dependent on other insulator proteins and in other cases independent. In this work, the authors have asked if CP190 associates with proteins that might explain its chromatin binding behavi ...
Document
... Microvilli (mv) are specialized actin-based structures Increases surface area for Absorption by20-fold Villin and fimbrin cross link 20-30 actin filaments in mv Villin introduced into fibroblasts Induces microvilli formation ...
... Microvilli (mv) are specialized actin-based structures Increases surface area for Absorption by20-fold Villin and fimbrin cross link 20-30 actin filaments in mv Villin introduced into fibroblasts Induces microvilli formation ...
Changes in DNA-binding activity of transcription factors in the
... Our earlier studies carried out in silico demonstrated that different transcription factors have their putative binding sites in the 5’-flanking regions of bovine milk protein genes. Now we extended our study to include the experimental analysis of these transcription factors. This study on electrop ...
... Our earlier studies carried out in silico demonstrated that different transcription factors have their putative binding sites in the 5’-flanking regions of bovine milk protein genes. Now we extended our study to include the experimental analysis of these transcription factors. This study on electrop ...
Cell biology # 2 - Nutley Public Schools
... • Na+ and K+ channels allow slow leakage down concentration gradients • Na+-K+ pump works as antiporter – Pumps against Na+ and K+ gradients to maintain high intracellular K+ concentration and high extracellular Na+ concentration • Maintains electrochemical gradients essential for functions of muscl ...
... • Na+ and K+ channels allow slow leakage down concentration gradients • Na+-K+ pump works as antiporter – Pumps against Na+ and K+ gradients to maintain high intracellular K+ concentration and high extracellular Na+ concentration • Maintains electrochemical gradients essential for functions of muscl ...
Calcium Signaling through Protein Kinases. The Arabidopsis
... three (Table I). The most conserved EF hand sequences are those of the hands in positions 1 and 2 and the least conserved is that for position 4. The positions where the EF hands are absent also vary. These differences in numbers and positions of EF hands likely yield variations in the allosteric pr ...
... three (Table I). The most conserved EF hand sequences are those of the hands in positions 1 and 2 and the least conserved is that for position 4. The positions where the EF hands are absent also vary. These differences in numbers and positions of EF hands likely yield variations in the allosteric pr ...
Top Ten Ways to Ensure Valid RNAi Data
... single nucleotide mismatch in the middle of an siRNA can abolish its activity [1,2]. In contrast, another report indicates that siRNAs can silence non-target genes containing as few as 14–15 consecutive complementary nucleotides [3]. Therefore, until we reach a better understanding of siRNA specific ...
... single nucleotide mismatch in the middle of an siRNA can abolish its activity [1,2]. In contrast, another report indicates that siRNAs can silence non-target genes containing as few as 14–15 consecutive complementary nucleotides [3]. Therefore, until we reach a better understanding of siRNA specific ...
Improving representation of biological responses
... GO:0050896 : response to stimulus ~ 150 direct EXP GO:0051716 : cellular response to stimulus ~ 5 direct EXP GO:0009628 : response to abiotic stimulus ~ 30 direct EXP GO:0009607 : response to biotic stimulus ~ 15 direct EXP GO:0042221 : response to chemical stimulus ~ 150 direct EXP GO:0009719 : res ...
... GO:0050896 : response to stimulus ~ 150 direct EXP GO:0051716 : cellular response to stimulus ~ 5 direct EXP GO:0009628 : response to abiotic stimulus ~ 30 direct EXP GO:0009607 : response to biotic stimulus ~ 15 direct EXP GO:0042221 : response to chemical stimulus ~ 150 direct EXP GO:0009719 : res ...
Supplementary Table 1 Entrez Gene (http://www.ncbi.nlm.nih.gov
... GTPase that switches between the active (GTPbound) state and inactive (GDP-bound) state to regulate CDC42 and other genes. Defects in this gene are a cause of startle disease with epilepsy (STHEE), also known as hyperekplexia with epilepsy. Three transcript variants encoding different isoforms have ...
... GTPase that switches between the active (GTPbound) state and inactive (GDP-bound) state to regulate CDC42 and other genes. Defects in this gene are a cause of startle disease with epilepsy (STHEE), also known as hyperekplexia with epilepsy. Three transcript variants encoding different isoforms have ...
Fractionation of rice glutelin polypeptides using gel filtration
... purifying crude glutelin extracts and obtaining preparative samplesof dissociatedglutelin polypeptides suitable for further characterization and study. Urea was the preferred extractant, becauseit would be less likely to interfere with further attempts at characterization and, if required, could be ...
... purifying crude glutelin extracts and obtaining preparative samplesof dissociatedglutelin polypeptides suitable for further characterization and study. Urea was the preferred extractant, becauseit would be less likely to interfere with further attempts at characterization and, if required, could be ...
Heme, Myoglobin, Hemoglobin
... heme oxygenase forming biliverdin and releasing iron. The iron can be transferred to apotransferrin (the iron transport protein) in plasma or can be stored within cells as ferritin (i.e. the iron is bound to the storage protein, apoferritin). The remaining porphyrin ring (biliverdin) is degraded to ...
... heme oxygenase forming biliverdin and releasing iron. The iron can be transferred to apotransferrin (the iron transport protein) in plasma or can be stored within cells as ferritin (i.e. the iron is bound to the storage protein, apoferritin). The remaining porphyrin ring (biliverdin) is degraded to ...
Signal Sequences Specify the Targeting Route to the Endoplasmic
... of ER-targeted proteins, the extent of the defect is variable from one protein to another: at one extreme, translocation of the vacuolar protease dipeptidyl aminopeptidase B (DPAP B) is severely affected; at the other extreme, carboxypeptidase Y (CPY) is unaffected (Hann and Walter, 1991). Furthermo ...
... of ER-targeted proteins, the extent of the defect is variable from one protein to another: at one extreme, translocation of the vacuolar protease dipeptidyl aminopeptidase B (DPAP B) is severely affected; at the other extreme, carboxypeptidase Y (CPY) is unaffected (Hann and Walter, 1991). Furthermo ...
genes is conserved among species related to
... Previously w e have shown that the type strain of Lactobacillus acidophilus possesses two S-protein-encoding genes, one of which is silent, on a chromosomal segment of 6 kb. The 5-protein-encoding gene in the expression site can be exchanged for the silent S-protein-encoding gene by inversion of t h ...
... Previously w e have shown that the type strain of Lactobacillus acidophilus possesses two S-protein-encoding genes, one of which is silent, on a chromosomal segment of 6 kb. The 5-protein-encoding gene in the expression site can be exchanged for the silent S-protein-encoding gene by inversion of t h ...
A Genomic Screen in Yeast Reveals Novel Aspects of
... at which individual steps occur can vary widely depending on the mRNA. However, it is currently not known which mechanisms target this basal degradation machinery preferentially to some mRNAs. In addition to affecting the expression of normal cellular genes, mRNA turnover also is important as a qual ...
... at which individual steps occur can vary widely depending on the mRNA. However, it is currently not known which mechanisms target this basal degradation machinery preferentially to some mRNAs. In addition to affecting the expression of normal cellular genes, mRNA turnover also is important as a qual ...
Sequence elements of the fusion peptide of human respiratory
... The HRSV F protein is a type I glycoprotein that is synthesized as an inactive precursor (F0) of 574 amino acids. This precursor is cleaved by furin-like proteases during maturation to yield two disulfide-linked polypeptides, F2 from the N terminus and F1 from the C terminus. The F0 precursor of HRS ...
... The HRSV F protein is a type I glycoprotein that is synthesized as an inactive precursor (F0) of 574 amino acids. This precursor is cleaved by furin-like proteases during maturation to yield two disulfide-linked polypeptides, F2 from the N terminus and F1 from the C terminus. The F0 precursor of HRS ...
Functional recognition of fragmented operator sites
... R17/MS2 coat protein. Our findings reveal that the coat protein can exert translational regulation well outside the confines of its RNA binding site. Coat proteins may also direct translational repression late in the infection by drawing RNA transcripts into capsid assemblies. This mode of translati ...
... R17/MS2 coat protein. Our findings reveal that the coat protein can exert translational regulation well outside the confines of its RNA binding site. Coat proteins may also direct translational repression late in the infection by drawing RNA transcripts into capsid assemblies. This mode of translati ...
Disruption of the Rice Plastid Ribosomal Protein S20 Leads to
... The rice albino seedling lethal mutant asl1 was derived from a 60Co gamma ray irradiated mutant pool of Oryza sativa cultivar Jiahua1 (wild type; WT). To generate a large F2 population for genetic analysis, heterozygous plants (ASL1/asl1) were crossed with the indica cultivar Pei’ai64S. Surface-ster ...
... The rice albino seedling lethal mutant asl1 was derived from a 60Co gamma ray irradiated mutant pool of Oryza sativa cultivar Jiahua1 (wild type; WT). To generate a large F2 population for genetic analysis, heterozygous plants (ASL1/asl1) were crossed with the indica cultivar Pei’ai64S. Surface-ster ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.