Plant Vacuoles
... Jauh et al., 1998). Distinctively, the membrane of the vegetative storage vacuoles contains the aquaporin d-TIP (Jauh et al., 1998; Neuhaus and Rogers, 1998). In the endosperm of cereal grains, proteins accumulate in endoplasmic reticulum (ER)–derived organelles of vacuole-like size (see below). A f ...
... Jauh et al., 1998). Distinctively, the membrane of the vegetative storage vacuoles contains the aquaporin d-TIP (Jauh et al., 1998; Neuhaus and Rogers, 1998). In the endosperm of cereal grains, proteins accumulate in endoplasmic reticulum (ER)–derived organelles of vacuole-like size (see below). A f ...
Operon Control of Gene Expression - Glebe
... are switched on and off together, as a unit. It is not the proteins that are produced that define an operon, an operon is a mechanism of control. An operon always contains several structural genes, an operator, and a promoter. ...
... are switched on and off together, as a unit. It is not the proteins that are produced that define an operon, an operon is a mechanism of control. An operon always contains several structural genes, an operator, and a promoter. ...
GFP - Bio-Rad
... Developed over five years, Biotechnology Explorer kits and curricula have been written for teachers, by teachers, and have been extensively field-tested in a broad range of classroom settings from high school through the undergraduate level. Easy-to-use Biotechnology Explorer kits are the perfect wa ...
... Developed over five years, Biotechnology Explorer kits and curricula have been written for teachers, by teachers, and have been extensively field-tested in a broad range of classroom settings from high school through the undergraduate level. Easy-to-use Biotechnology Explorer kits are the perfect wa ...
Copy number variations involving the microtubule
... phospholipase activity, and with the Abelson kinase [34]. Interestingly, Abelson is widely expressed but is notably highly expressed in hyaline cartilage [35], which could give us a link to the dysmorphic features involving notably the nose in affected patients. To conclude, both deletions and dupli ...
... phospholipase activity, and with the Abelson kinase [34]. Interestingly, Abelson is widely expressed but is notably highly expressed in hyaline cartilage [35], which could give us a link to the dysmorphic features involving notably the nose in affected patients. To conclude, both deletions and dupli ...
Identification of a novel Endoplasmic Reticulum Stress Response
... similarity to the Endoplasmic Reticulum Stress Response Element (ERSE). This novel ERSE (“ERSE-26”) is able to regulate PRNP endogenously in response to endoplasmic reticulum (ER) stress. In order to determine whether or not the ERSE-26 exists elsewhere in the genome and what is co-regulated with PR ...
... similarity to the Endoplasmic Reticulum Stress Response Element (ERSE). This novel ERSE (“ERSE-26”) is able to regulate PRNP endogenously in response to endoplasmic reticulum (ER) stress. In order to determine whether or not the ERSE-26 exists elsewhere in the genome and what is co-regulated with PR ...
simposi sobre infertilitat masculina: genètica i ambient
... biology. aneuploidy, or an abnormal chromosome number, is a factor in ~35% of spontaneous pregnancy losses, and is the most common recognized cause of mental retardation. The frequency of aneuploidy among oocytes can reach 20 to 25%, and increases among aging women. In contrast, chromosome abnormali ...
... biology. aneuploidy, or an abnormal chromosome number, is a factor in ~35% of spontaneous pregnancy losses, and is the most common recognized cause of mental retardation. The frequency of aneuploidy among oocytes can reach 20 to 25%, and increases among aging women. In contrast, chromosome abnormali ...
Lecture_8
... -stiff connections between actin-binding domains Spectrin and filamin form actin filament webs of gels -flexible connections between actin-binding domains (filamanin) -stiff bent connection (spectrin) ...
... -stiff connections between actin-binding domains Spectrin and filamin form actin filament webs of gels -flexible connections between actin-binding domains (filamanin) -stiff bent connection (spectrin) ...
Cloning and expression of the phosphotriesterase
... Michaelis–Menten kinetics were observed with the oxon OPs, but not with the thion OPs. End-product inhibition was observed for coroxon-hydrolytic activity. Increased expression of hocA was observed from an integrative hocA–lacZ fusion when cultures were grown in the absence of phosphate, suggesting ...
... Michaelis–Menten kinetics were observed with the oxon OPs, but not with the thion OPs. End-product inhibition was observed for coroxon-hydrolytic activity. Increased expression of hocA was observed from an integrative hocA–lacZ fusion when cultures were grown in the absence of phosphate, suggesting ...
Molecular Mechanisms of Long Noncoding RNAs
... of multiple genes by interacting with chromatin and recruiting the chromatin-modifying machinery. For example, in mouse placenta, lncRNAs such as Air and Kcnq1ot1 accumulate at promoter chromatin of silenced alleles and mediate repressive histone modifications in an allele-specific manner (Mohammad ...
... of multiple genes by interacting with chromatin and recruiting the chromatin-modifying machinery. For example, in mouse placenta, lncRNAs such as Air and Kcnq1ot1 accumulate at promoter chromatin of silenced alleles and mediate repressive histone modifications in an allele-specific manner (Mohammad ...
BIM1 Encodes a Microtubule-binding Protein in Yeast.
... also was found to have more subtle defects in spindle morphology (Trueheart et al., 1987). However, bik1 mutants display synthetic lethality with tubulin mutations (Berlin et al., 1990) as well as mutations in other genes. A particularly interesting genetic characteristic of BIK1 is that overexpress ...
... also was found to have more subtle defects in spindle morphology (Trueheart et al., 1987). However, bik1 mutants display synthetic lethality with tubulin mutations (Berlin et al., 1990) as well as mutations in other genes. A particularly interesting genetic characteristic of BIK1 is that overexpress ...
video slide - Wayne State University
... Now, here is one of the central problems of biology. Much of what is going on in a cell, as we have seen, is carried out by proteins. There are all kinds of proteins, depending on the sequence of their 20 amino acids: ...
... Now, here is one of the central problems of biology. Much of what is going on in a cell, as we have seen, is carried out by proteins. There are all kinds of proteins, depending on the sequence of their 20 amino acids: ...
Dynamic properties of a reconstituted myelin sheath
... MBP is essential for the formation of CNS myelin, able to interact with a wide range of ligands, often polyanionic in nature [2]. Due to its high charge and low overall hydrophobicity, there is significant intramolecular electrostatic repulsion in MBP; this results in MBP being an extended, intrinsi ...
... MBP is essential for the formation of CNS myelin, able to interact with a wide range of ligands, often polyanionic in nature [2]. Due to its high charge and low overall hydrophobicity, there is significant intramolecular electrostatic repulsion in MBP; this results in MBP being an extended, intrinsi ...
1. Introduction Chemotaxis Chemotaxis is the net movement of a
... MA domain. It consists of two methylation helices where the adaptation proteins interact and a highly conserved region at the hinge necessary for interaction with CheW and CheA. The methylation helices contain conserved glutamate (or glutamine) residues available for modification via a variety of ad ...
... MA domain. It consists of two methylation helices where the adaptation proteins interact and a highly conserved region at the hinge necessary for interaction with CheW and CheA. The methylation helices contain conserved glutamate (or glutamine) residues available for modification via a variety of ad ...
Urine Reflection of Changes in Blood
... compared with 33,930 articles found using [(‘urine’ OR ‘urinary’) and ‘biomarker’]. In reality, the number of articles related to protein biomarkers in urine is no more than 450 in a manually organized database (until June 2013). There have already been some data, which indicates that certain urine ...
... compared with 33,930 articles found using [(‘urine’ OR ‘urinary’) and ‘biomarker’]. In reality, the number of articles related to protein biomarkers in urine is no more than 450 in a manually organized database (until June 2013). There have already been some data, which indicates that certain urine ...
types of gel - WordPress.com
... Agarose gels are made from the natural polysaccharide polymers extracted from seaweed. Agarose gels are easily cast and handled compared to other matrices, because the gel setting is a physical rather than chemical change. Samples are also easily recovered. After the experiment is finished, the resu ...
... Agarose gels are made from the natural polysaccharide polymers extracted from seaweed. Agarose gels are easily cast and handled compared to other matrices, because the gel setting is a physical rather than chemical change. Samples are also easily recovered. After the experiment is finished, the resu ...
Jonathan Kindberg
... understand the protein interactions in the nervous system of both humans and C. elegans. This animal is a good candidate for experimental procedures because it has a simple nervous system and it uses similar neurotransmitters that are used by the human brain. When C. elegans is exposed to a continuo ...
... understand the protein interactions in the nervous system of both humans and C. elegans. This animal is a good candidate for experimental procedures because it has a simple nervous system and it uses similar neurotransmitters that are used by the human brain. When C. elegans is exposed to a continuo ...
Trans - Wiley
... The splicing machinery is recruited to introncontaining transcripts co-transcriptionally. ...
... The splicing machinery is recruited to introncontaining transcripts co-transcriptionally. ...
Dictyostelium lysosomal proteins with different sugar modifications
... enrichment of glycoproteins containing these modifications, respectively (Fig. 2). Since most of the CP and glycosidase activities were recovered after magnetic fractionation, we conclude that these enzymes are in vesicles that are recruited to the endo-lysosomal pathway and that paramagnetic fracti ...
... enrichment of glycoproteins containing these modifications, respectively (Fig. 2). Since most of the CP and glycosidase activities were recovered after magnetic fractionation, we conclude that these enzymes are in vesicles that are recruited to the endo-lysosomal pathway and that paramagnetic fracti ...
Document
... Utilise known structure/function information to infer facts related to the predicted protein sequence Provide users with results from a number of standard algorithms/searches Provide users with cross-references (dbxrefs) to other resources Assign a simple one line description for each gene product ...
... Utilise known structure/function information to infer facts related to the predicted protein sequence Provide users with results from a number of standard algorithms/searches Provide users with cross-references (dbxrefs) to other resources Assign a simple one line description for each gene product ...
Comparitive Genomics- Final Results
... •Vibrio vulnificus pathogenecity - increased iron in the host results in increased susceptibility to infection (Weinberg 2000). ...
... •Vibrio vulnificus pathogenecity - increased iron in the host results in increased susceptibility to infection (Weinberg 2000). ...
Slow Protein Conformational Change, Allostery and
... protein-protein interaction. There is no exaggeration to say that the Michaelis–Menten model has greatly pushed forward our understanding of enzymatic reactions. However, biochemists also found that many enzymes show kinetics are more complicated than the Michaelis-Menten kinetics. Frieden coined th ...
... protein-protein interaction. There is no exaggeration to say that the Michaelis–Menten model has greatly pushed forward our understanding of enzymatic reactions. However, biochemists also found that many enzymes show kinetics are more complicated than the Michaelis-Menten kinetics. Frieden coined th ...
A Discussion of the Mechanism of Action of Steroid Hormones*
... the environment of their substrates. If this is true in muscle, one might ask whether a vestige of such a control mechanism might also be operative in other tissues. Accessory factors such as pH, equilibrium con stant, oxidation-reduction potential, and free en ergy are also implicitly involved. Sim ...
... the environment of their substrates. If this is true in muscle, one might ask whether a vestige of such a control mechanism might also be operative in other tissues. Accessory factors such as pH, equilibrium con stant, oxidation-reduction potential, and free en ergy are also implicitly involved. Sim ...
28. biosynthesis of proteins
... ribosomal proteins ; 20 or more enzymes to activate the amino acid precursors; about 15 auxiliary enzymes and other specific protein factors for initiation, elongation and termination of polypeptides; perhaps 100 additional enzymes for the final processing of different kinds of proteins and 40 or mo ...
... ribosomal proteins ; 20 or more enzymes to activate the amino acid precursors; about 15 auxiliary enzymes and other specific protein factors for initiation, elongation and termination of polypeptides; perhaps 100 additional enzymes for the final processing of different kinds of proteins and 40 or mo ...
The Chlamydomonas genome project: a decade on
... As a unicellular haploid in the vegetative stage of its life cycle, Chlamydomonas shares the experimental advantages associated with microbes. These include rapid doubling time (8–12 h), well-defined media and growth requirements, the ability to synchronize cultures with periodic light exposure, th ...
... As a unicellular haploid in the vegetative stage of its life cycle, Chlamydomonas shares the experimental advantages associated with microbes. These include rapid doubling time (8–12 h), well-defined media and growth requirements, the ability to synchronize cultures with periodic light exposure, th ...
Mol Microbiol. Jun 21. [Epub ahead of print]
... From a public health perspective, the mixed species biofilms that form in anthropogenic water systems are well known and important environmental reservoirs for L. pneumophila growth (Declerck et al., 2009), and some recent studies have provided insights into biofilm formation by L. pneumophila. It h ...
... From a public health perspective, the mixed species biofilms that form in anthropogenic water systems are well known and important environmental reservoirs for L. pneumophila growth (Declerck et al., 2009), and some recent studies have provided insights into biofilm formation by L. pneumophila. It h ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.