Phenotypic Heterogeneity Enables Uropathogenic Escherichia coli
... determined in 96-well microtiter plate. Growth was assessed after incubation for 18 h, and the MIC value was determined. Serum and antibiotic sensitivity. Cells from DMSO stock were diluted 1:100 in filtered LB medium and grown aerobically to the stationary phase (20 h). Cells were harvested, washed ...
... determined in 96-well microtiter plate. Growth was assessed after incubation for 18 h, and the MIC value was determined. Serum and antibiotic sensitivity. Cells from DMSO stock were diluted 1:100 in filtered LB medium and grown aerobically to the stationary phase (20 h). Cells were harvested, washed ...
Terminal Cytokinesis Events Uncovered after an RNAi Screen
... separation of daughter cells. Rather, as first described by Flemming in 1891 (see [7]), a persistent intercellular bridge forms around the spindle remnant; this bridge is marked at its center by a darkly staining structure, or midbody (e.g., Figure 1A, insert). This bridge remains long after furrowi ...
... separation of daughter cells. Rather, as first described by Flemming in 1891 (see [7]), a persistent intercellular bridge forms around the spindle remnant; this bridge is marked at its center by a darkly staining structure, or midbody (e.g., Figure 1A, insert). This bridge remains long after furrowi ...
Fibronectin and radial intercalation
... Keller and Jansa have further shown that involution and dorsal mesoderm extension occur even in the absence of a BCR (Keller and Jansa, 1992), suggesting that the primary mechanism regulating dorsal mesoderm movement in Xenopus is convergent extension. However, others have reported that inhibition o ...
... Keller and Jansa have further shown that involution and dorsal mesoderm extension occur even in the absence of a BCR (Keller and Jansa, 1992), suggesting that the primary mechanism regulating dorsal mesoderm movement in Xenopus is convergent extension. However, others have reported that inhibition o ...
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... from the ablated blastomere are simply missing, while the remaining blastomeres give rise to their normal complements of progeny cells (Blair and Weisblat, 1982; Blair, 1983). However, cell fate may be less rigidly determined for epidermal cells; following ablation of one ectodermal teloblast (0, P ...
... from the ablated blastomere are simply missing, while the remaining blastomeres give rise to their normal complements of progeny cells (Blair and Weisblat, 1982; Blair, 1983). However, cell fate may be less rigidly determined for epidermal cells; following ablation of one ectodermal teloblast (0, P ...
Arabidopsis R-SNARE Proteins VAMP721 and VAMP722 Are
... AtCDC48 has been proposed, as AtCDC48 specifically interacts with SYP31 but not with KNOLLE in vitro-binding assay in spite of the colocalization at cell-division plane between SYP31 or AtCDC48 and KNOLLE [17]. To date, only NPSN11, one RSNARE candidate for cell-plate membrane fusion machinery, has ...
... AtCDC48 has been proposed, as AtCDC48 specifically interacts with SYP31 but not with KNOLLE in vitro-binding assay in spite of the colocalization at cell-division plane between SYP31 or AtCDC48 and KNOLLE [17]. To date, only NPSN11, one RSNARE candidate for cell-plate membrane fusion machinery, has ...
Apigenin
... the confrontation of tumour cell spheroids with chick embryo heart fragments. HeLa Cx43 cells were highly invasive in controls, but did not invade the heart tissue at tumour cell aggregate-fibroblast capsule interfaces in the presence of apigenin and failed to fully engulf these heart fragments. Bec ...
... the confrontation of tumour cell spheroids with chick embryo heart fragments. HeLa Cx43 cells were highly invasive in controls, but did not invade the heart tissue at tumour cell aggregate-fibroblast capsule interfaces in the presence of apigenin and failed to fully engulf these heart fragments. Bec ...
Living together in biofilms: the microbial cell factory and its
... lactic acid, and succinic acid) or during wastewater treatment or bioremediation. Thus far, the biofilms used to obtain industrial products are typically those of single species, which allows the controlled growth conditions needed to maximize the production of the desired compound [59–61]. In biofi ...
... lactic acid, and succinic acid) or during wastewater treatment or bioremediation. Thus far, the biofilms used to obtain industrial products are typically those of single species, which allows the controlled growth conditions needed to maximize the production of the desired compound [59–61]. In biofi ...
Power-Assisted Liposuction (PAL) vs. Traditional Liposuction
... improvements in technique/instrumentation and selection of optimal candidates are critical to maintain its safety profile and effectiveness1. Since the introduction of power-assisted liposuction (PAL) by MicroAire Surgical Instruments (FDA 510(K) December 1998, the device has undergone developmental ...
... improvements in technique/instrumentation and selection of optimal candidates are critical to maintain its safety profile and effectiveness1. Since the introduction of power-assisted liposuction (PAL) by MicroAire Surgical Instruments (FDA 510(K) December 1998, the device has undergone developmental ...
Cell shrinkage and apoptosis: a role for potassium and sodium ion
... impact on investigations into cell death and our understanding of embryonic development and tissue homeostasis. The most common and best described form of this type of cell death (programmed cell death) is apoptosis (Kerr et al, 1972), which can occur either from developmentally controlled activatio ...
... impact on investigations into cell death and our understanding of embryonic development and tissue homeostasis. The most common and best described form of this type of cell death (programmed cell death) is apoptosis (Kerr et al, 1972), which can occur either from developmentally controlled activatio ...
Early Morphogenesis of the Caenorhabditis elegans Pharynx
... cavity, and mouth into close apposition (“Contraction”). Several models can account for these cellular behaviors, and we distinguish between them by physically or genetically ablating cells within the digestive tract. These studies provide the first description of how the pharynx primordium develops ...
... cavity, and mouth into close apposition (“Contraction”). Several models can account for these cellular behaviors, and we distinguish between them by physically or genetically ablating cells within the digestive tract. These studies provide the first description of how the pharynx primordium develops ...
Intracellular and extracellular in vivo recording of
... inactivated; the membrane must be hyperpolarized below this level for at least 50-100 ms to de-inactivate the conductance so that the next sufficiently large depolarization will activate it. This low threshold spike typically has a high frequency burst of 2-7 conventional action potentials riding on ...
... inactivated; the membrane must be hyperpolarized below this level for at least 50-100 ms to de-inactivate the conductance so that the next sufficiently large depolarization will activate it. This low threshold spike typically has a high frequency burst of 2-7 conventional action potentials riding on ...
The Cellular Basis of Gastrulation in Xenopus laevis: Active
... and pull everything inside (Holtfreter, 1943a, b). However, there are several arguments against this mechanism operating in anurans (see Keller, 1981) and urodeles (Daniel and Yarwood, 1939). In Xenopus, an anuran, bottle cell removal does not prevent extension, convergence, or involution (Cooke, 19 ...
... and pull everything inside (Holtfreter, 1943a, b). However, there are several arguments against this mechanism operating in anurans (see Keller, 1981) and urodeles (Daniel and Yarwood, 1939). In Xenopus, an anuran, bottle cell removal does not prevent extension, convergence, or involution (Cooke, 19 ...
Metabolic Basis of Visual Cycle Inhibition by Retinoid and
... FIGURE 1. Structures of visual cycle inhibitors and isoprenoids. Based on their chemical structures, the bp) and A0 (5⬘-CCACAGCACACstudied compounds are classified into four groups: retinoic acid derivatives (A), positively charged retinoids (B), ATCAGCATTTCTCC-3⬘) and N1 farnesyl-containing isopren ...
... FIGURE 1. Structures of visual cycle inhibitors and isoprenoids. Based on their chemical structures, the bp) and A0 (5⬘-CCACAGCACACstudied compounds are classified into four groups: retinoic acid derivatives (A), positively charged retinoids (B), ATCAGCATTTCTCC-3⬘) and N1 farnesyl-containing isopren ...
Constructing a Plant Cell. The Genetic Control of Root Hair
... Multicellular organisms possess a diverse array of cell types, which vary in size, shape, composition, and function. Therefore, a fundamental feature of development in multicellular organisms is the proper specification and differentiation of distinct cell types. In plants, the formation of hair cel ...
... Multicellular organisms possess a diverse array of cell types, which vary in size, shape, composition, and function. Therefore, a fundamental feature of development in multicellular organisms is the proper specification and differentiation of distinct cell types. In plants, the formation of hair cel ...
Links between DNA Replication, Stem Cells and Cancer
... brain [11,12], prostate [12], and colon [13]. These two theories are not mutually exclusive, because CSCs might arise during mammalian development through the accumulation of genetic mutations. Alternatively, CSCs might represent quiescent stem cells that eventually awaken within an alien environmen ...
... brain [11,12], prostate [12], and colon [13]. These two theories are not mutually exclusive, because CSCs might arise during mammalian development through the accumulation of genetic mutations. Alternatively, CSCs might represent quiescent stem cells that eventually awaken within an alien environmen ...
Cell Death Suppressor, Arabidopsis BI
... penetration by Blumeria graminis (Hückelhoven et al., 2003), suggesting that AtBI-1 and Mlo may possess similar functions in cellular defense and cell death modulation. Moreover, it was demonstrated that the barley Mlo protein binds to calmodulin (HvCaM3) and the interaction is related to the contro ...
... penetration by Blumeria graminis (Hückelhoven et al., 2003), suggesting that AtBI-1 and Mlo may possess similar functions in cellular defense and cell death modulation. Moreover, it was demonstrated that the barley Mlo protein binds to calmodulin (HvCaM3) and the interaction is related to the contro ...
Targeting lactate metabolism for cancer therapeutics
... transcription of the glutamine transporter ASC-like Na+-dependent neutral amino acid transporter (ASCT2; also known as SLC1a5) and by repressing microRNA 23a/b (miR-23a/b), which normally blocks GLS translation (13, 14). Higher GLS expression results in increased glutamine uptake and catabolism, aga ...
... transcription of the glutamine transporter ASC-like Na+-dependent neutral amino acid transporter (ASCT2; also known as SLC1a5) and by repressing microRNA 23a/b (miR-23a/b), which normally blocks GLS translation (13, 14). Higher GLS expression results in increased glutamine uptake and catabolism, aga ...
Recovery of the resurrection plant Craterostigma wilmsii from
... Fig. 4. (A) Mesophyll cell of slowly dried C. wilmsii tissue rehydrated to 100% RWC in distamycin A. Vacuole (v); chloroplasts (c). Note the folding of the mesophyll cell wall (w) and electron dense material in the vacuole. (B) Chloroplast of slowly dried C. wilmsii tissue rehydrated to 100% RWC in ...
... Fig. 4. (A) Mesophyll cell of slowly dried C. wilmsii tissue rehydrated to 100% RWC in distamycin A. Vacuole (v); chloroplasts (c). Note the folding of the mesophyll cell wall (w) and electron dense material in the vacuole. (B) Chloroplast of slowly dried C. wilmsii tissue rehydrated to 100% RWC in ...
Andrei Fagarasanu Department of Cell Biology Supervisor: Dr
... Many internationally recognized scientists and clinicians have studied at “Carol Davila” University, including Nicolae Paulescu, Victor Babes, Thoma Ionescu and George Palade, who is considered the father of modern cell biology. While a medical student, I participated as a teaching assistant in anat ...
... Many internationally recognized scientists and clinicians have studied at “Carol Davila” University, including Nicolae Paulescu, Victor Babes, Thoma Ionescu and George Palade, who is considered the father of modern cell biology. While a medical student, I participated as a teaching assistant in anat ...
Phosphatidylserine, a death knell
... acts on the extracytosolic, external face of the plasma membrane to regulate intercellular interactions. Appreciation of this new role for phospholipids was galvanized by the demonstration that phosphatidylserine (PS) appears on the surface of apoptotic lymphocytes and contributes to their phagocyto ...
... acts on the extracytosolic, external face of the plasma membrane to regulate intercellular interactions. Appreciation of this new role for phospholipids was galvanized by the demonstration that phosphatidylserine (PS) appears on the surface of apoptotic lymphocytes and contributes to their phagocyto ...
IL-15 and dermal fibroblasts induce proliferation of natural
... Tregs from blood.24 To determine whether the CD4⫹CD25hiCD69lo T cells we isolated from skin were functional Tregs, we isolated these cells by flow sorting (Figure 2B) and tested them for the ability to suppress the proliferation of CD25lo T cells isolated from the same sample of skin. CD25hiCD69lo T ...
... Tregs from blood.24 To determine whether the CD4⫹CD25hiCD69lo T cells we isolated from skin were functional Tregs, we isolated these cells by flow sorting (Figure 2B) and tested them for the ability to suppress the proliferation of CD25lo T cells isolated from the same sample of skin. CD25hiCD69lo T ...
SLP-76-Cbl-Grb2-Shc Interactions in Fc RI Signaling
... activation of Ras.9-17 It is ubiquitously expressed and occurs in two isoforms of 46 kD and 52 kD in hematopoetic cells. The Shc protein contains an amino-terminal phosphotyrosinebinding (PTB) domain, a central collagen homology (CH) region, and a carboxyl-terminal SH2 domain, but no apparent cataly ...
... activation of Ras.9-17 It is ubiquitously expressed and occurs in two isoforms of 46 kD and 52 kD in hematopoetic cells. The Shc protein contains an amino-terminal phosphotyrosinebinding (PTB) domain, a central collagen homology (CH) region, and a carboxyl-terminal SH2 domain, but no apparent cataly ...
Cytoplasmic streaming enables the distribution of molecules
... be said to be large or small, the competition is defined in these terms. One such quantity is the Reynolds number Re measuring the importance of inertia to viscous drag, with turbulent flows occurring at very high Re. In contrast, most processes that occur within cells or involving motility of indiv ...
... be said to be large or small, the competition is defined in these terms. One such quantity is the Reynolds number Re measuring the importance of inertia to viscous drag, with turbulent flows occurring at very high Re. In contrast, most processes that occur within cells or involving motility of indiv ...
IGF-1 induces rat glomerular mesangial cells to accumulate
... Submitted 8 February 2005; accepted in final form 25 July 2005 ...
... Submitted 8 February 2005; accepted in final form 25 July 2005 ...
Cytoplasmic streaming enables the distribution of molecules
... be said to be large or small, the competition is defined in these terms. One such quantity is the Reynolds number Re measuring the importance of inertia to viscous drag, with turbulent flows occurring at very high Re. In contrast, most processes that occur within cells or involving motility of indiv ...
... be said to be large or small, the competition is defined in these terms. One such quantity is the Reynolds number Re measuring the importance of inertia to viscous drag, with turbulent flows occurring at very high Re. In contrast, most processes that occur within cells or involving motility of indiv ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.