Why Belzer UW® Cold Storage Solution?
... “Various impermeants have been used to sustain the oncotic gradient across the cell membrane and prevent oedema. … UW pioneered the use of lactobionate and raffinose…Lactobionate remains the impermeant choice of having been successfully used in preservation solutions other than UW, being critical to ...
... “Various impermeants have been used to sustain the oncotic gradient across the cell membrane and prevent oedema. … UW pioneered the use of lactobionate and raffinose…Lactobionate remains the impermeant choice of having been successfully used in preservation solutions other than UW, being critical to ...
D:\ACTA\3-2003\jozwiak 732.vp
... ther 72 h. Then MTT was added and incubation was continued for a further 4 h. After aspiration of the culture medium, the resulting formazan crystals were dissolved in dimethyl sulfoxide and absorbance was immediately read at 570 nm. The fraction of surviving cells was determined by dividing the mea ...
... ther 72 h. Then MTT was added and incubation was continued for a further 4 h. After aspiration of the culture medium, the resulting formazan crystals were dissolved in dimethyl sulfoxide and absorbance was immediately read at 570 nm. The fraction of surviving cells was determined by dividing the mea ...
Molecular profiling of single circulating tumor cells with diagnostic
... mean ratios of observed over expected CTCs (Supplementary Table S1), we determined a mean transfer efficiency of 85% (median 77%, standard deviation 49%). Patient CTCs displayed heterogeneous morphology and staining intensity as in previous studies (Allard et al, 2004) adding observer variability ...
... mean ratios of observed over expected CTCs (Supplementary Table S1), we determined a mean transfer efficiency of 85% (median 77%, standard deviation 49%). Patient CTCs displayed heterogeneous morphology and staining intensity as in previous studies (Allard et al, 2004) adding observer variability ...
Roles of hepatic stellate cells in liver inflammation: a new perspective
... (VCAM-1)), and Sele (E-selectin). Decreased expression of these genes may have ameliorated hepatocyte damage, sinusoidal leukocyte accumulation, and hemostasis in ConA- and BW245C-treated mice. In addition, our study also indicates that HSCs control CD4+ T cell trafficking to liver parenchyma. While ...
... (VCAM-1)), and Sele (E-selectin). Decreased expression of these genes may have ameliorated hepatocyte damage, sinusoidal leukocyte accumulation, and hemostasis in ConA- and BW245C-treated mice. In addition, our study also indicates that HSCs control CD4+ T cell trafficking to liver parenchyma. While ...
Identification and characterization of subpopulations
... Identification and characterization of subpopulations in undifferentiated ES cell culture Yayoi Toyooka1,2,*, Daisuke Shimosato1,3, Kazuhiro Murakami1, Kadue Takahashi1 and Hitoshi Niwa1,3,* Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass (ICM) and the epiblast, and ...
... Identification and characterization of subpopulations in undifferentiated ES cell culture Yayoi Toyooka1,2,*, Daisuke Shimosato1,3, Kazuhiro Murakami1, Kadue Takahashi1 and Hitoshi Niwa1,3,* Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass (ICM) and the epiblast, and ...
Molecular profiling of single circulating tumor cells with
... mean ratios of observed over expected CTCs (Supplementary Table S1), we determined a mean transfer efficiency of 85% (median 77%, standard deviation 49%). Patient CTCs displayed heterogeneous morphology and staining intensity as in previous studies (Allard et al, 2004) adding observer variability ...
... mean ratios of observed over expected CTCs (Supplementary Table S1), we determined a mean transfer efficiency of 85% (median 77%, standard deviation 49%). Patient CTCs displayed heterogeneous morphology and staining intensity as in previous studies (Allard et al, 2004) adding observer variability ...
figure 2 - Open Biology
... requires a systems-level understanding of the signalling networks that respond to external cues and regulate the cytoskeleton. Classical biochemical and genetic approaches have identified thousands of individual components that contribute to cell shape, but it remains difficult to predict how cell s ...
... requires a systems-level understanding of the signalling networks that respond to external cues and regulate the cytoskeleton. Classical biochemical and genetic approaches have identified thousands of individual components that contribute to cell shape, but it remains difficult to predict how cell s ...
isolation and genetic analysis of mutant strains in gametic
... by R. K. TOGASAKI and taken to Indiana University, where they were maintained in culture. Subsequently, DR. TOGASAKI sent us cells from his cultures (which we propose to designate 137c-H mt+ and 137c-H mt-). Both groups of investigators proceeded to use these as parent strains for the isolation of v ...
... by R. K. TOGASAKI and taken to Indiana University, where they were maintained in culture. Subsequently, DR. TOGASAKI sent us cells from his cultures (which we propose to designate 137c-H mt+ and 137c-H mt-). Both groups of investigators proceeded to use these as parent strains for the isolation of v ...
Reversal of Physiological Stress
... Department of Pharmacology, The George Washington University Medical Center, Washington, DC Received March 2, 2001; accepted May 22, 2001 ...
... Department of Pharmacology, The George Washington University Medical Center, Washington, DC Received March 2, 2001; accepted May 22, 2001 ...
Expression of a Nondegradable Cyclin B1 Affects
... S. cerevisiae (budding yeast), high levels of nondegradable cyclin CLB2 arrest cells late in mitosis, with segregated chromosomes and the presence of an elongated mitotic spindle (Surana et al., 1993). Indestructible cyclin Cdc13 arrests Schizosaccharomyces pombe cells in anaphase with separated and ...
... S. cerevisiae (budding yeast), high levels of nondegradable cyclin CLB2 arrest cells late in mitosis, with segregated chromosomes and the presence of an elongated mitotic spindle (Surana et al., 1993). Indestructible cyclin Cdc13 arrests Schizosaccharomyces pombe cells in anaphase with separated and ...
Expression of a Nondegradable Cyclin B1 Affects
... S. cerevisiae (budding yeast), high levels of nondegradable cyclin CLB2 arrest cells late in mitosis, with segregated chromosomes and the presence of an elongated mitotic spindle (Surana et al., 1993). Indestructible cyclin Cdc13 arrests Schizosaccharomyces pombe cells in anaphase with separated and ...
... S. cerevisiae (budding yeast), high levels of nondegradable cyclin CLB2 arrest cells late in mitosis, with segregated chromosomes and the presence of an elongated mitotic spindle (Surana et al., 1993). Indestructible cyclin Cdc13 arrests Schizosaccharomyces pombe cells in anaphase with separated and ...
Expression of the chicken GATA factor family during
... results obtained have proven concordant with abundances detected by RNA blot analysis when directly compared (e.g. Braga et al., 1992). We initially examined cGATA expression in a variety of avian hematopoietic cells in order to compare it with prior results as a test of the validity of the RT-PCR m ...
... results obtained have proven concordant with abundances detected by RNA blot analysis when directly compared (e.g. Braga et al., 1992). We initially examined cGATA expression in a variety of avian hematopoietic cells in order to compare it with prior results as a test of the validity of the RT-PCR m ...
The hypersensitive response is associated with host and nonhost
... Late blight, caused by the oomycete Phytophthora infestans, is the most devastating disease of potato (Solanum tuberosum) world-wide. To limit chemical control, breeding potato to incorporate durable forms of genetic resistance is needed. The genus Solanum comprises an extensive gene pool, in which ...
... Late blight, caused by the oomycete Phytophthora infestans, is the most devastating disease of potato (Solanum tuberosum) world-wide. To limit chemical control, breeding potato to incorporate durable forms of genetic resistance is needed. The genus Solanum comprises an extensive gene pool, in which ...
TGF beta signalling and its role in tumour pathogenesis
... it to the extracellular matrix. Cellular recognition of extracellular matrix-associated LTBP and subsequent recognition of latent TGF-β are essential steps in its activation (Hyytiainen et al., 2004). The latency proteins also contribute to the cytokine stability. Free TGF-β has a half life of about ...
... it to the extracellular matrix. Cellular recognition of extracellular matrix-associated LTBP and subsequent recognition of latent TGF-β are essential steps in its activation (Hyytiainen et al., 2004). The latency proteins also contribute to the cytokine stability. Free TGF-β has a half life of about ...
Correlation between Innervation of Skeletal Muscles and
... a stress reaction to denervation after a one-week lag phase, and they began to divide, proliferate and differentiate in a great quantity in order to relieve the cellular atrophy caused by denervation; however, as the tissue always stayed in a state of denervation, the SCs were unable to fend for the ...
... a stress reaction to denervation after a one-week lag phase, and they began to divide, proliferate and differentiate in a great quantity in order to relieve the cellular atrophy caused by denervation; however, as the tissue always stayed in a state of denervation, the SCs were unable to fend for the ...
Nephridia
... (i) Micro and Meganephridia. On the basis of their size and number, nephridia may be micronephridia or meganephridia. Micronephridia or meronephridia or smaller in size and are numerous in each segment (Pheretima). Meganephridia or holonephridia are larger in size and generally or pair per segment. ...
... (i) Micro and Meganephridia. On the basis of their size and number, nephridia may be micronephridia or meganephridia. Micronephridia or meronephridia or smaller in size and are numerous in each segment (Pheretima). Meganephridia or holonephridia are larger in size and generally or pair per segment. ...
Isolated Osteoclasts Resorb the Organic and Inorganic Components
... electron microscopy, these cells were seen to have the abundant mitochondria and the extensive membrane ruffling characteristic of osteoclasts (Fig. 2). Binding of osteoclast-specific antibody confirmed that the cells were osteoclasts (Fig. 3). Isolated osteoclasts, incubated with 45Ca o r L-[5-aH]p ...
... electron microscopy, these cells were seen to have the abundant mitochondria and the extensive membrane ruffling characteristic of osteoclasts (Fig. 2). Binding of osteoclast-specific antibody confirmed that the cells were osteoclasts (Fig. 3). Isolated osteoclasts, incubated with 45Ca o r L-[5-aH]p ...
The Par3/Par6/aPKC Complex and Epithelial Cell Polarity
... In addition to its role in forming the Par complex, Par3 also has Par6/aPKC-independent functions. Par3 contains a conserved N-terminal domain (NTD, also called CR1), three central PDZ domains, and the C-terminal region containing multiple protein binding sites including the aPKC-binding-motif. The ...
... In addition to its role in forming the Par complex, Par3 also has Par6/aPKC-independent functions. Par3 contains a conserved N-terminal domain (NTD, also called CR1), three central PDZ domains, and the C-terminal region containing multiple protein binding sites including the aPKC-binding-motif. The ...
Manipulation of oxidative protein folding and PDI redox state in
... incubated for different times in the absence of the reducing agent. Each sample was treated with AMS and the electrophoretic mobility of PDI analysed under nonreducing conditions. Consistent with the results shown in Figure 5B, PDI was more oxidized in Ero1-La overexpressing cells (Figure 5C, compar ...
... incubated for different times in the absence of the reducing agent. Each sample was treated with AMS and the electrophoretic mobility of PDI analysed under nonreducing conditions. Consistent with the results shown in Figure 5B, PDI was more oxidized in Ero1-La overexpressing cells (Figure 5C, compar ...
Manipulation of oxidative protein folding and PDI redox state in
... incubated for different times in the absence of the reducing agent. Each sample was treated with AMS and the electrophoretic mobility of PDI analysed under nonreducing conditions. Consistent with the results shown in Figure 5B, PDI was more oxidized in Ero1-La overexpressing cells (Figure 5C, compar ...
... incubated for different times in the absence of the reducing agent. Each sample was treated with AMS and the electrophoretic mobility of PDI analysed under nonreducing conditions. Consistent with the results shown in Figure 5B, PDI was more oxidized in Ero1-La overexpressing cells (Figure 5C, compar ...
Chapter 11. Growth
... point out that sustained growth associated with these low pH's would still require the synthesis of new wall polymers and recent studies have been conflicting, some claiming that the role for protons was an artifact of the experimental technique (Schopfer, 1989; Vanderhoef et at., 1977a) and others ...
... point out that sustained growth associated with these low pH's would still require the synthesis of new wall polymers and recent studies have been conflicting, some claiming that the role for protons was an artifact of the experimental technique (Schopfer, 1989; Vanderhoef et at., 1977a) and others ...
Augmin Plays a Critical Role in Organizing the
... augmin complex, fly dim g-tubulin 4 (Dgt4) and human augminlike complex, subunit 8 (HAUS8)/HICE1 are MT-associated proteins (MAPs) and are likely responsible for establishing the augmin–MT interaction (Wu et al., 2008; Uehara et al., 2009). Because no obvious homologs of NEDD1/GCP-WD or augmin have ...
... augmin complex, fly dim g-tubulin 4 (Dgt4) and human augminlike complex, subunit 8 (HAUS8)/HICE1 are MT-associated proteins (MAPs) and are likely responsible for establishing the augmin–MT interaction (Wu et al., 2008; Uehara et al., 2009). Because no obvious homologs of NEDD1/GCP-WD or augmin have ...
A Genome-Wide Screen for Bacterial Envelope Biogenesis Mutants
... The cell envelope of bacteria serves as their interface with the environment. This structure plays an essential role in maintaining cellular integrity and provide protection from external insults. For pathogens, the envelope is the site of first contact with the host and where major pathogenicity de ...
... The cell envelope of bacteria serves as their interface with the environment. This structure plays an essential role in maintaining cellular integrity and provide protection from external insults. For pathogens, the envelope is the site of first contact with the host and where major pathogenicity de ...
Synovial fibroblasts: key players in rheumatoid
... This notion has mainly been derived from studies in the severe combined immunodeficient mouse (SCID) co-implantation model [10, 11]. This animal model for RA was developed to investigate several aspects of an affected human joint under controlled conditions in vivo. Briefly, human RASF and fresh human ...
... This notion has mainly been derived from studies in the severe combined immunodeficient mouse (SCID) co-implantation model [10, 11]. This animal model for RA was developed to investigate several aspects of an affected human joint under controlled conditions in vivo. Briefly, human RASF and fresh human ...
High-throughput screens for fluorescent dye discovery
... mark an antigen has become one of the most common staining techniques for fluorescence microscopy because of its versatility: fairly specific antibodies can be developed for most proteins. If enough is known about a biological pathway, an investigator can readily create antibodies to proteins known ...
... mark an antigen has become one of the most common staining techniques for fluorescence microscopy because of its versatility: fairly specific antibodies can be developed for most proteins. If enough is known about a biological pathway, an investigator can readily create antibodies to proteins known ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.