Reactive Oxygen Intermediates Activate NF-KB in a
... pre-T-cell line CEM. The radiation sensitivityofthese cell lines was detailed in a previous report.18 Irradiation and treatments of cells. Cells (5 X 105/mL)in plastic tissue culture flasks were irradiated with 500 to 2,000 cGy at a dose rate of 100 cGy/min during log phase and under aerobic conditi ...
... pre-T-cell line CEM. The radiation sensitivityofthese cell lines was detailed in a previous report.18 Irradiation and treatments of cells. Cells (5 X 105/mL)in plastic tissue culture flasks were irradiated with 500 to 2,000 cGy at a dose rate of 100 cGy/min during log phase and under aerobic conditi ...
Reactive Oxygen Intermediates Activate NF-KB in a
... pre-T-cell line CEM. The radiation sensitivityofthese cell lines was detailed in a previous report.18 Irradiation and treatments of cells. Cells (5 X 105/mL)in plastic tissue culture flasks were irradiated with 500 to 2,000 cGy at a dose rate of 100 cGy/min during log phase and under aerobic conditi ...
... pre-T-cell line CEM. The radiation sensitivityofthese cell lines was detailed in a previous report.18 Irradiation and treatments of cells. Cells (5 X 105/mL)in plastic tissue culture flasks were irradiated with 500 to 2,000 cGy at a dose rate of 100 cGy/min during log phase and under aerobic conditi ...
Characterization of transport systems for cysteine, lysine, alanine
... Despite the obvious importance of AA nutrition to fiber production, few attempts have been made to characterize the AA transport systems that operate in the wool follicle to supply the large quantity and unique ...
... Despite the obvious importance of AA nutrition to fiber production, few attempts have been made to characterize the AA transport systems that operate in the wool follicle to supply the large quantity and unique ...
Long-Term Persistent Vesicular Stomatitis Virus and Rabies Virus
... virus for the establishment of persistence, and it was shown that a long T particle produced continuously by the carrier B H K 2i cells could lead to the establishment of persistence whenever it infected B H K aI cells along with B virions. We have now monitored the B H K 21 vsgind CAR4 carrier cell ...
... virus for the establishment of persistence, and it was shown that a long T particle produced continuously by the carrier B H K 2i cells could lead to the establishment of persistence whenever it infected B H K aI cells along with B virions. We have now monitored the B H K 21 vsgind CAR4 carrier cell ...
Restricted, canonical, stereotyped and convergent immunoglobulin
... B-cell responses of C57BL/6 mice to NP select for canonical antibodies encoded by V186.2 recombined to DFI 16.1 D segment and the JH1 J segment that are paired with Vl1 light chains. It had been assumed that the simplistic nature of the hapten antigens in combination with the inbred mice led to the ...
... B-cell responses of C57BL/6 mice to NP select for canonical antibodies encoded by V186.2 recombined to DFI 16.1 D segment and the JH1 J segment that are paired with Vl1 light chains. It had been assumed that the simplistic nature of the hapten antigens in combination with the inbred mice led to the ...
21 intraovarian sperm storage in helicolenus dactylopterus
... for this study during the course of a year. They were analysed in order to observe the ultrastructure of the storage crypts with stored spermatozoa and to describe their evolution over the year. After internal fertilization and once sperm reaches the ovary, a crypt forms probably by an epithelial in ...
... for this study during the course of a year. They were analysed in order to observe the ultrastructure of the storage crypts with stored spermatozoa and to describe their evolution over the year. After internal fertilization and once sperm reaches the ovary, a crypt forms probably by an epithelial in ...
Protoplast Culture: definition
... protoplasts/cm3. Protoplasts fail to divide when plated at one tenth of this concentration. ...
... protoplasts/cm3. Protoplasts fail to divide when plated at one tenth of this concentration. ...
... al., 1997). The size and location of the presumptive eye field is normal in cyclops mutant and morphant embryos; however, hypothalamic tissue is not induced (Fig. 4D, see Fig. S1 in the supplementary material) (Varga et al., 1999). Tracking cells in cyc morphants, we show that the focus of neural-pl ...
Paramecium trichocysts isolated with their membranes are stable in
... We have shown that it is possible to isolate Paramecium trichocysts with their membranes by a simple and rapid procedure. We used tam6 mutant cells whose functional trichocysts are free in the cytoplasm, and self-forming Percoll buoyant density gradients, which permit rapid, high-resolution separati ...
... We have shown that it is possible to isolate Paramecium trichocysts with their membranes by a simple and rapid procedure. We used tam6 mutant cells whose functional trichocysts are free in the cytoplasm, and self-forming Percoll buoyant density gradients, which permit rapid, high-resolution separati ...
negative Drug-resistant Cell Lines Using Laser
... the chamber was treated with 1 mg/ml poly-L-lysine for 15 min to facilitate cell adhesion. Loosely bound cells were then washed free from the microscope slide with buffer equilibrated to the appropriate tem perature, and then a glass cover slide was placed on top of the chamber. Cells adhering to th ...
... the chamber was treated with 1 mg/ml poly-L-lysine for 15 min to facilitate cell adhesion. Loosely bound cells were then washed free from the microscope slide with buffer equilibrated to the appropriate tem perature, and then a glass cover slide was placed on top of the chamber. Cells adhering to th ...
Caveolin-3 and SAP97 form a scaffolding protein complex that
... the PDZ1 and PDZ2 domains followed by a proline-rich region and part of PDZ3 (Fig. 2A); and SAP97(1–200), which contained the NH2-terminal multimerization domain (not shown). Each of these constructs was cotransfected with Cav-3 and tested for interaction with it in coimmunoprecipitation assays usin ...
... the PDZ1 and PDZ2 domains followed by a proline-rich region and part of PDZ3 (Fig. 2A); and SAP97(1–200), which contained the NH2-terminal multimerization domain (not shown). Each of these constructs was cotransfected with Cav-3 and tested for interaction with it in coimmunoprecipitation assays usin ...
Movements and stepwise fusion of endodermal precursor cells in
... A′′′, B′′′ and C′′′ (Nardelli-Haefliger and Shankland 1993; Smith et al. 1996). We report here that the macromeres undergo stepwise cell fusions and marked positional changes prior to and during epiboly. Prior to epiboly in Helobdella, stereotyped cleavages produce three types of cells, macromeres, ...
... A′′′, B′′′ and C′′′ (Nardelli-Haefliger and Shankland 1993; Smith et al. 1996). We report here that the macromeres undergo stepwise cell fusions and marked positional changes prior to and during epiboly. Prior to epiboly in Helobdella, stereotyped cleavages produce three types of cells, macromeres, ...
Journal of Plant Growth Regulation
... the biotic world was classified into two main divisions, plants and animals, on the basis of the conspicuous differences in their anatomy, morphology, behavior, and ecology. In the twentieth century, with ever increasingly sophisticated tools of analysis, cellular-level similarities between these tw ...
... the biotic world was classified into two main divisions, plants and animals, on the basis of the conspicuous differences in their anatomy, morphology, behavior, and ecology. In the twentieth century, with ever increasingly sophisticated tools of analysis, cellular-level similarities between these tw ...
The Mechanisms of hsp27 Antibody-Mediated Apoptosis in Retinal
... (Fig. 1 A). Using control mouse antibodies, anti-IgG or anticalbindin-D, immunogold labeling was negative in retinal neuronal cells (Fig. 1 B,C). The internalization of exogenously applied hsp27 antibody exhibited a time-dependent pattern. In retina incubated with hsp27 antibody for 30 min, immunogo ...
... (Fig. 1 A). Using control mouse antibodies, anti-IgG or anticalbindin-D, immunogold labeling was negative in retinal neuronal cells (Fig. 1 B,C). The internalization of exogenously applied hsp27 antibody exhibited a time-dependent pattern. In retina incubated with hsp27 antibody for 30 min, immunogo ...
Action Potential Transfer in Cell Pairs Isolated From Adult Rat and
... sociating cardiac tissue (e.g., see Trube12) enabled a novel approach. Besides single cells, these methods also yield functionally intact cell pairs (e.g., see Kameyama13 and Metzger and Weingart14). Such cell pairs enable impulse transmission to be investigated at the cellular level for the first t ...
... sociating cardiac tissue (e.g., see Trube12) enabled a novel approach. Besides single cells, these methods also yield functionally intact cell pairs (e.g., see Kameyama13 and Metzger and Weingart14). Such cell pairs enable impulse transmission to be investigated at the cellular level for the first t ...
![[9] John Hiscott1,2,3,4, Hakju Kwon1,2 and Pierre Génin1,2. Hostile](http://s1.studyres.com/store/data/009149009_1-886f14ff4241c04e7687727f6a979f26-300x300.png)
[9] John Hiscott1,2,3,4, Hakju Kwon1,2 and Pierre Génin1,2. Hostile
... JME/CF15 cells, calu-3 cells grew to confluence with greater uniformity and with cleaner, more defined monolayers than those observed with the JME/CF15 cell line. The slow growth of the calu-3 cells along with their resistance to transfection precluded their use in roGFP experiments. Calu-3 cells we ...
... JME/CF15 cells, calu-3 cells grew to confluence with greater uniformity and with cleaner, more defined monolayers than those observed with the JME/CF15 cell line. The slow growth of the calu-3 cells along with their resistance to transfection precluded their use in roGFP experiments. Calu-3 cells we ...
Essays in Biochemistry Volume 39 Chapter 1
... demonstrated that exactly 131 of the 1090 somatic cells generated during development underwent programmed cell death. Both the lineage of each dying cell and the time that it underwent programmed cell death were highly reproducible, and these observations highlighted the tightly regulated nature of ...
... demonstrated that exactly 131 of the 1090 somatic cells generated during development underwent programmed cell death. Both the lineage of each dying cell and the time that it underwent programmed cell death were highly reproducible, and these observations highlighted the tightly regulated nature of ...
HAUSP, a deubiquitinating enzyme for p53, is polyubiquitinated
... regions between nucleosomes. As expected, rHAUSP-transfected HeLa cells died completely. We observed extracted genomic DNA from apoptotic HeLa cells induced by rHAUSP and rHAUSP (C224S), visualized as DNA ladders, indicating that these cells are apoptotic (data not shown). We also investigated the e ...
... regions between nucleosomes. As expected, rHAUSP-transfected HeLa cells died completely. We observed extracted genomic DNA from apoptotic HeLa cells induced by rHAUSP and rHAUSP (C224S), visualized as DNA ladders, indicating that these cells are apoptotic (data not shown). We also investigated the e ...
Basic Research Grants Funded for 2012
... times. In the first set of experiments, we will increase SMN everywhere in the body but at different times. This will allow us to bypass potential problems that might exist in one organ system or another. In the second set of experiments we will only increase SMN in the spinal cord. This experiment ...
... times. In the first set of experiments, we will increase SMN everywhere in the body but at different times. This will allow us to bypass potential problems that might exist in one organ system or another. In the second set of experiments we will only increase SMN in the spinal cord. This experiment ...
A Golgi and tonoplast localized Sacyl transferase is involved in cell
... AtPAT10 is an S-acyl transferase Yeast AKR1p has been shown to be an S-acyl transferase (Roth et al., 2002). The loss-of-function mutant akr1 shows various temperature-sensitive defects including elongated multinucleate cells and poor viability when grown at 37°C. At 25°C near normal growth occurs ( ...
... AtPAT10 is an S-acyl transferase Yeast AKR1p has been shown to be an S-acyl transferase (Roth et al., 2002). The loss-of-function mutant akr1 shows various temperature-sensitive defects including elongated multinucleate cells and poor viability when grown at 37°C. At 25°C near normal growth occurs ( ...
Multiscale Systems Analysis of Root Growth and
... descriptions have had essentially phenomenological components: Relating the quantities that appear within them to the cellular and subcellular properties is a crucial and challenging aspect of multiscale mechanistic modeling. Systematic mathematical procedures (associated with homogenization theory ...
... descriptions have had essentially phenomenological components: Relating the quantities that appear within them to the cellular and subcellular properties is a crucial and challenging aspect of multiscale mechanistic modeling. Systematic mathematical procedures (associated with homogenization theory ...
Patterns of Collective Bacterial Motion in Microfluidic Devices
... We have studied active and passive forms of pattern formation and synchronized motion in high-density bacterial cell suspensions with phase contrast and epifluorescent videomicroscopy. We have shown that in high-density cultures of non-motile cells sedimentation patterns form. By comparing bacterial ...
... We have studied active and passive forms of pattern formation and synchronized motion in high-density bacterial cell suspensions with phase contrast and epifluorescent videomicroscopy. We have shown that in high-density cultures of non-motile cells sedimentation patterns form. By comparing bacterial ...
Visualizing microtubule dynamics and membrane - UvA-DARE
... EMM tomography revealed the existence of at least two different kinds of vesicles during celll plate formation which could suggest the involvement of multiple membrane sources andd trafficking pathways driving cell plate formation (Segui-Simarro et al., 2004). Inn addition to these observations, rec ...
... EMM tomography revealed the existence of at least two different kinds of vesicles during celll plate formation which could suggest the involvement of multiple membrane sources andd trafficking pathways driving cell plate formation (Segui-Simarro et al., 2004). Inn addition to these observations, rec ...
Localization of Green Fluorescent Protein Fusions
... approaches. Highly purified pea CCVs contained abundant BP-80 (Kirsch et al., 1994). Immunocytochemical electron microscope (immunoEM) studies with specific antibodies also localized BP-80 and its Arabidopsis homolog, AtELP, to both Golgi and a putative PVC in root-tip cells of pea and Arabidopsis, ...
... approaches. Highly purified pea CCVs contained abundant BP-80 (Kirsch et al., 1994). Immunocytochemical electron microscope (immunoEM) studies with specific antibodies also localized BP-80 and its Arabidopsis homolog, AtELP, to both Golgi and a putative PVC in root-tip cells of pea and Arabidopsis, ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.