Wnt Signaling Translocates Lys48-Linked
... ligases such as b-TrCP, triggering polyubiquitination and degradation in proteasomes (Acebron et al., 2014). GSK3 is a major cellular serine/threonine kinase with many substrates (Kim et al., 2009). In bioinformatic analyses, about 20% of human proteins were found to contain three or more consecutiv ...
... ligases such as b-TrCP, triggering polyubiquitination and degradation in proteasomes (Acebron et al., 2014). GSK3 is a major cellular serine/threonine kinase with many substrates (Kim et al., 2009). In bioinformatic analyses, about 20% of human proteins were found to contain three or more consecutiv ...
Development of neural tube basal lamina during neurulation and
... examined by transmission electron microscopy (TEM) the entire trunk from the posterior neuropore to the cervical region. From this spectrum, we have chosen six levels to illustrate the development of BL (Fig. 1A). At the most posterior levels, the future NC cells are found within the ridges of the e ...
... examined by transmission electron microscopy (TEM) the entire trunk from the posterior neuropore to the cervical region. From this spectrum, we have chosen six levels to illustrate the development of BL (Fig. 1A). At the most posterior levels, the future NC cells are found within the ridges of the e ...
Archives of Microbiology
... and K o l t e r 1992); (3) other proteins that are i n d u c e d b y o x i d a t i v e stress, o s m o t i c stress, heat s h o c k (i.e., D n a K , G r o E L and G r p E chaperonins); and (4) stationary phase i n d u c i b l e proteins (Sip) ( A l e x a n d e r and St. Johns 1994). T h e e x p r e ...
... and K o l t e r 1992); (3) other proteins that are i n d u c e d b y o x i d a t i v e stress, o s m o t i c stress, heat s h o c k (i.e., D n a K , G r o E L and G r p E chaperonins); and (4) stationary phase i n d u c i b l e proteins (Sip) ( A l e x a n d e r and St. Johns 1994). T h e e x p r e ...
BYSTANDER HELP IN PRIMARY IMMUNE RESPONSES IN
... titer of Fl-specific antibody in B10, B10.A, and BALB.B mice (Fig. 2). Both antigens must be injected in the same anatomical location, but there is no requirement for mixing of the antigens before immunization . Separate intraperitoneal injections of each antigen will induce hapten-specific antibody ...
... titer of Fl-specific antibody in B10, B10.A, and BALB.B mice (Fig. 2). Both antigens must be injected in the same anatomical location, but there is no requirement for mixing of the antigens before immunization . Separate intraperitoneal injections of each antigen will induce hapten-specific antibody ...
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... examined by transmission electron microscopy (TEM) the entire trunk from the posterior neuropore to the cervical region. From this spectrum, we have chosen six levels to illustrate the development of BL (Fig. 1A). At the most posterior levels, the future NC cells are found within the ridges of the e ...
... examined by transmission electron microscopy (TEM) the entire trunk from the posterior neuropore to the cervical region. From this spectrum, we have chosen six levels to illustrate the development of BL (Fig. 1A). At the most posterior levels, the future NC cells are found within the ridges of the e ...
Neurogenesis in Postnatal Mouse Dorsal Root Ganglia
... 2 weeks in serum-free medium containing bFGF, neurons were rarely observed. However, following removal of bFGF and addition of trophic factors, many cells were observed that morphologically resembled dorsal root ganglia neurons, stained for neuronal markers, and generated action potentials. Furtherm ...
... 2 weeks in serum-free medium containing bFGF, neurons were rarely observed. However, following removal of bFGF and addition of trophic factors, many cells were observed that morphologically resembled dorsal root ganglia neurons, stained for neuronal markers, and generated action potentials. Furtherm ...
Mechanisms That Promote Stem Cell Maintenance throughout Life
... cells and to document their ability to self-renew for a prolonged period. Seven different types of stem cell have now been identified (Figure 1; Table 1). In contrast to the ability to identify invertebrate stem cells and their niches with single-cell resolution, the relative vastness of mammalian t ...
... cells and to document their ability to self-renew for a prolonged period. Seven different types of stem cell have now been identified (Figure 1; Table 1). In contrast to the ability to identify invertebrate stem cells and their niches with single-cell resolution, the relative vastness of mammalian t ...
Histochemical Polymorphism of Keratin Pearls in Squamous Cell
... In compliance with the objectives of the study a complex of histological and histochemical methods has been applied during the investigation. The analysis has been carried out on post operational material, taken from 25 patients with keratinizing squamous cell carcinoma of lung. The obtained materia ...
... In compliance with the objectives of the study a complex of histological and histochemical methods has been applied during the investigation. The analysis has been carried out on post operational material, taken from 25 patients with keratinizing squamous cell carcinoma of lung. The obtained materia ...
In vitro analysis of the VE-cadherin promoter - HAL
... shown to play a crucial role in vessel assembly (Carmeliet et al., 1999; Corada et al., 1999; Corada et al., 2002; Gory-Fauré et al., 1999; Liao et al., 2002) and endothelial permeability (Corada et al., 2001; Gao et al., 2000; Gotsch et al., 1997; Gulino et al., 1998; Hordijk et al., 1999; Wong et ...
... shown to play a crucial role in vessel assembly (Carmeliet et al., 1999; Corada et al., 1999; Corada et al., 2002; Gory-Fauré et al., 1999; Liao et al., 2002) and endothelial permeability (Corada et al., 2001; Gao et al., 2000; Gotsch et al., 1997; Gulino et al., 1998; Hordijk et al., 1999; Wong et ...
TR3 is preferentially expressed by bulge epithelial stem
... Figure 2 TR3 and K15 expression in cultured bulge keratinocytes. (a) Morphology of a keratinocyte colony derived from cell suspension of trypsinized telogen bulge follicles, stained with H&E. (b) Morphology of a keratinocyte colony derived from cell suspension of trypsinized hair matrix cells, stain ...
... Figure 2 TR3 and K15 expression in cultured bulge keratinocytes. (a) Morphology of a keratinocyte colony derived from cell suspension of trypsinized telogen bulge follicles, stained with H&E. (b) Morphology of a keratinocyte colony derived from cell suspension of trypsinized hair matrix cells, stain ...
Mouse mammary tumor virus-based vector transduces non
... mammary gland. It is not clear how the prototypic betaretrovirus infects mucosal DCs and naïve lymphocytes as these cells are considered to be non-proliferative. Studies of MMTV biology have been hampered by the difficulty of obtaining sufficient virus/vector titers after transfection of a molecular ...
... mammary gland. It is not clear how the prototypic betaretrovirus infects mucosal DCs and naïve lymphocytes as these cells are considered to be non-proliferative. Studies of MMTV biology have been hampered by the difficulty of obtaining sufficient virus/vector titers after transfection of a molecular ...
Primary Culture of Identified Neurons from the Visual Cortex of
... Visual cortex from rat pups 1-15 d old was subjected to enzymatic and mechanical dissociation to yield a suspension of single cells. Neurons plated onto collagen or a feeder layer of astrocytes rapidly extended processes and survived for 4-10 weeks. Antisera to glutamic acid decarboxylase, choline a ...
... Visual cortex from rat pups 1-15 d old was subjected to enzymatic and mechanical dissociation to yield a suspension of single cells. Neurons plated onto collagen or a feeder layer of astrocytes rapidly extended processes and survived for 4-10 weeks. Antisera to glutamic acid decarboxylase, choline a ...
Meristem-Specific Suppression of Mitosis and a
... tinuous turnover of the cap (Clowes, 1972, 1994; Barlow, 1973). Consistent with such a model was the assumption that such so-called “sloughed root cap cells” are waste products that are programmed to die and in fact begin to degenerate even before separation from the root (Haberlandt, 1914; Rougier, ...
... tinuous turnover of the cap (Clowes, 1972, 1994; Barlow, 1973). Consistent with such a model was the assumption that such so-called “sloughed root cap cells” are waste products that are programmed to die and in fact begin to degenerate even before separation from the root (Haberlandt, 1914; Rougier, ...
Antigen Export during Liver Infection of the Malaria - edoc
... CFSElow CD45.2⫹ CD8⫹ cells in these organs (Fig. 3C). In both organs, CD8⫹ T cells isolated from mice immunized with expOVA parasites showed the highest proliferation. A substantial proportion (~10 to 25%) of CD45.2⫹ CD8⫹ T cells recruited to the liver proliferated upon infection with OVA sporozoite ...
... CFSElow CD45.2⫹ CD8⫹ cells in these organs (Fig. 3C). In both organs, CD8⫹ T cells isolated from mice immunized with expOVA parasites showed the highest proliferation. A substantial proportion (~10 to 25%) of CD45.2⫹ CD8⫹ T cells recruited to the liver proliferated upon infection with OVA sporozoite ...
Interferon-α (IFN-α) suppresses HTLV-1 gene
... Background: Human T-cell leukemia virus type-1 (HTLV-1) is the causative retrovirus of adult T-cell leukemia/ lymphoma (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). HTLV-1 gene expression is maintained at low levels in vivo by unknown mechanisms. A combination therap ...
... Background: Human T-cell leukemia virus type-1 (HTLV-1) is the causative retrovirus of adult T-cell leukemia/ lymphoma (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). HTLV-1 gene expression is maintained at low levels in vivo by unknown mechanisms. A combination therap ...
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... capturing exosome by antibody (2). Finally, exosome itself has a huge capacity for disease treatment. To do this, isolating exosome from cells that originate from patients themselves to avoid immuno-reaction caused by foreign substances. In this context, adipose tissue derived mesenchymal stem cells ...
... capturing exosome by antibody (2). Finally, exosome itself has a huge capacity for disease treatment. To do this, isolating exosome from cells that originate from patients themselves to avoid immuno-reaction caused by foreign substances. In this context, adipose tissue derived mesenchymal stem cells ...
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... Microvilli (mv) are specialized actin-based structures Increases surface area for Absorption by20-fold Villin and fimbrin cross link 20-30 actin filaments in mv Villin introduced into fibroblasts Induces microvilli formation ...
... Microvilli (mv) are specialized actin-based structures Increases surface area for Absorption by20-fold Villin and fimbrin cross link 20-30 actin filaments in mv Villin introduced into fibroblasts Induces microvilli formation ...
PDF - Eleanor Maine Research Lab
... Stem cells achieve self-renewal through both the execution of mitotic cell division and maintenance of stem cell fate. Different stem cell types display distinct patterns of self-renewal and differentiation. For example, hematopoetic stem cells divide infrequently compared with ES cells (Orford and ...
... Stem cells achieve self-renewal through both the execution of mitotic cell division and maintenance of stem cell fate. Different stem cell types display distinct patterns of self-renewal and differentiation. For example, hematopoetic stem cells divide infrequently compared with ES cells (Orford and ...
Pedomicrobium - International Journal of Systematic and
... colonies are often lysed, giving a granular appearance to the colonies. These colonies have a cartilaginous consistency and may be removed from the agar intact. After restreaking on solid media, type 1 or 2 colonies give rise to both types of colonies. With periodic transfers to fresh medium type 1 ...
... colonies are often lysed, giving a granular appearance to the colonies. These colonies have a cartilaginous consistency and may be removed from the agar intact. After restreaking on solid media, type 1 or 2 colonies give rise to both types of colonies. With periodic transfers to fresh medium type 1 ...
NIH Public Access
... positive subset of multipotent progenitors, and is absent in differentiated cells. Targeted disruption of Sox9 leads to severe pancreatic hypoplasia due to progenitor depletion, a phenotype similar to Notch mutations. Sox9 is involved in the maintenance of the pancreatic progenitor pool and does so ...
... positive subset of multipotent progenitors, and is absent in differentiated cells. Targeted disruption of Sox9 leads to severe pancreatic hypoplasia due to progenitor depletion, a phenotype similar to Notch mutations. Sox9 is involved in the maintenance of the pancreatic progenitor pool and does so ...
OCTOPUS, a polarly localised membrane
... The development of the vasculature starts during embryogenesis and repeats itself in every newly forming or growing organ. In a first step, which is referred to as vascular patterning, the position of the vasculature is laid down. During this process, provascular cells, uncommitted meristematic cell ...
... The development of the vasculature starts during embryogenesis and repeats itself in every newly forming or growing organ. In a first step, which is referred to as vascular patterning, the position of the vasculature is laid down. During this process, provascular cells, uncommitted meristematic cell ...
Cultivation of Trypanosoma brucei sspp. in
... trypanosomes (Vickerman, 1971 ; Newton, Cross & Baker, 1973) has been tempered by problems associated with the cultivation of these organisms. The Salivaria are among the most difficult trypanosomes to cultivate in vitro. Of the various cell types occurring during the natural life-cycle, only those ...
... trypanosomes (Vickerman, 1971 ; Newton, Cross & Baker, 1973) has been tempered by problems associated with the cultivation of these organisms. The Salivaria are among the most difficult trypanosomes to cultivate in vitro. Of the various cell types occurring during the natural life-cycle, only those ...
Transforming growth factor‐β1 suppresses hepatitis B virus
... established by stably transfecting HepG2 cells with a 1.3fold HBV genome.26 The 1.3ES2 cells were chosen because of the production of viral replicative intermediates and its capacity to support cccDNA formation. To identify the effective dose that interfered with HBV replication, various concentrati ...
... established by stably transfecting HepG2 cells with a 1.3fold HBV genome.26 The 1.3ES2 cells were chosen because of the production of viral replicative intermediates and its capacity to support cccDNA formation. To identify the effective dose that interfered with HBV replication, various concentrati ...
A novel live cell assay to measure diacylglycerol lipase α activity
... processes including axonal growth and guidance, adult neurogenesis and retrograde signalling at the synapse. Recent studies have implicated DAGLα as an emerging drug target for several conditions including pain and obesity. Activity assays are critical to the drug discovery process; however, measure ...
... processes including axonal growth and guidance, adult neurogenesis and retrograde signalling at the synapse. Recent studies have implicated DAGLα as an emerging drug target for several conditions including pain and obesity. Activity assays are critical to the drug discovery process; however, measure ...
An EMMPRIN–c-catenin–Nm23 complex drives ATP production and
... characterized in relation to tumorigenesis and inflammation (Muramatsu and Miyauchi, 2003). EMMPRIN can also contribute to angiogenesis, the formation of new vessels, in several ways: (1) by endothelial cell activation when present on tumor-derived microvesicles (Millimaggi et al., 2007), (2) by reg ...
... characterized in relation to tumorigenesis and inflammation (Muramatsu and Miyauchi, 2003). EMMPRIN can also contribute to angiogenesis, the formation of new vessels, in several ways: (1) by endothelial cell activation when present on tumor-derived microvesicles (Millimaggi et al., 2007), (2) by reg ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.