Discs large 5, an Essential Gene in Drosophila, Regulates Egg
... dlg5 clones were smaller than control clones (not shown), supporting the idea that loss of dlg5 affects cell division or cell viability. From these results we can conclude that Dlg5 is essential in both germline and somatic stem cells, and it is likely required in all follicle cells throughout oogen ...
... dlg5 clones were smaller than control clones (not shown), supporting the idea that loss of dlg5 affects cell division or cell viability. From these results we can conclude that Dlg5 is essential in both germline and somatic stem cells, and it is likely required in all follicle cells throughout oogen ...
Gutsy moves in mice: cellular and molecular dynamics of endoderm
... embryo. As increasing numbers of cells undergo this process, the newly formed sheet of DE cells would form a contiguous epithelium with the VE, which would gradually and uniformly displace the latter to proximal regions, from where it would exclusively contribute to extraembryonic tissues. Additiona ...
... embryo. As increasing numbers of cells undergo this process, the newly formed sheet of DE cells would form a contiguous epithelium with the VE, which would gradually and uniformly displace the latter to proximal regions, from where it would exclusively contribute to extraembryonic tissues. Additiona ...
Activation of DNA Damage Response Pathways during Lytic
... Like all herpesviruses, KSHV has a double-stranded DNA genome and a biphasic lifecycle that consists of latent and lytic stages. During latent infection, the virus persists as an extrachromosomal episome that is replicated by host replication machinery. Latency is characterised by expression of a li ...
... Like all herpesviruses, KSHV has a double-stranded DNA genome and a biphasic lifecycle that consists of latent and lytic stages. During latent infection, the virus persists as an extrachromosomal episome that is replicated by host replication machinery. Latency is characterised by expression of a li ...
Chapter 12 The Cell Cycle Multiple-Choice Questions
... B) They never coil up their chromosomes when they are dividing. C) They use mitotic division but only have circular chromosomes. D) They maintain a nuclear envelope during division. E) None of them form spindles. Answer: D Topic: Concept 12.2 Skill: Application/Analysis ...
... B) They never coil up their chromosomes when they are dividing. C) They use mitotic division but only have circular chromosomes. D) They maintain a nuclear envelope during division. E) None of them form spindles. Answer: D Topic: Concept 12.2 Skill: Application/Analysis ...
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... widespread candidature. The question papers are closely related and the relationships between them have been thoroughly established using our assessment expertise. All versions of the paper give assessment of equal standard. The content assessed by the examination papers and the type of questions is ...
implications for key virulence factors in Flavobacterium columnare
... whereas these were missing from the biofilm OMVs, indicating that planktonic cells may be important mediators of disease [13]. The role of OMVs has also been studied extensively in many other pathogenic bacteria, and there is no doubt of their significant role in the virulence of bacterial pathogens ...
... whereas these were missing from the biofilm OMVs, indicating that planktonic cells may be important mediators of disease [13]. The role of OMVs has also been studied extensively in many other pathogenic bacteria, and there is no doubt of their significant role in the virulence of bacterial pathogens ...
Physiological effects of long-term energy
... stabilized at 0.50 and remained constant throughout the remainder of the study. ATP remained nearly constant for 2 wk then decreased until 4 wk when it stabilized at 0.85 fmol ml-' at 10"ells ml-' N. cryotolerans appears to be well adapted to energy-source deprivation. It is able to conserve energy ...
... stabilized at 0.50 and remained constant throughout the remainder of the study. ATP remained nearly constant for 2 wk then decreased until 4 wk when it stabilized at 0.85 fmol ml-' at 10"ells ml-' N. cryotolerans appears to be well adapted to energy-source deprivation. It is able to conserve energy ...
The chromosome partitioning protein, ParB, is required for
... influenzae ), most of our information regarding their function comes from elegant work on the E. coli bacteriophage P1, which exists as a circular episome when a prophage, and the F-factor plasmid. Both of these genetic elements exist as single-copy episomes and are inherited by each daughter cell w ...
... influenzae ), most of our information regarding their function comes from elegant work on the E. coli bacteriophage P1, which exists as a circular episome when a prophage, and the F-factor plasmid. Both of these genetic elements exist as single-copy episomes and are inherited by each daughter cell w ...
Chapter 7: Cellular Structure and Function - Bellbrook
... ago. According to the endosymbiont theory, a symbiotic mutual relationship involved one prokaryotic cell living inside of another. The endosymbiont theory is discussed in greater detail in Chapter 14. Imagine how organisms would be different if the eukaryotic form had not evolved. Because eukaryotic ...
... ago. According to the endosymbiont theory, a symbiotic mutual relationship involved one prokaryotic cell living inside of another. The endosymbiont theory is discussed in greater detail in Chapter 14. Imagine how organisms would be different if the eukaryotic form had not evolved. Because eukaryotic ...
Association of Calmodulin and an Unconventional Myosin with the
... the eukaryotic microorganism Dictyostelium discoideum. Calmodulin is a small, highly conserved, calcium-binding protein found in all eukaryotic cells; it has been implicated in the regulation of many aspects of cellular metabolism and cell motility (reviewed in Manalan and Klee, 1984; Cohen and Klee ...
... the eukaryotic microorganism Dictyostelium discoideum. Calmodulin is a small, highly conserved, calcium-binding protein found in all eukaryotic cells; it has been implicated in the regulation of many aspects of cellular metabolism and cell motility (reviewed in Manalan and Klee, 1984; Cohen and Klee ...
msc_botnay_pre_pap1_bl1 - Madhya Pradesh Bhoj Open
... The internal atmosphere of cell differs from that of its external environment. This difference is maintain throughout the life of the cell by thin surface membrane. The plasma membrane which controls the entrance & exit of molecules & ions. The capacity of the plasma membrane to act as a selective b ...
... The internal atmosphere of cell differs from that of its external environment. This difference is maintain throughout the life of the cell by thin surface membrane. The plasma membrane which controls the entrance & exit of molecules & ions. The capacity of the plasma membrane to act as a selective b ...
The homeobox-containing gene Wariai regulates anterior
... al., 1995; Morris et al., 1987; Town et al., 1976; Williams et al., 1987; Jermyn et al., 1987; Berks and Kay, 1990). The expression of prestalk-specific genes is mediated by a STAT transcription factor (Kawata et al., 1997); however, the role of DIF in potentially regulating STAT function is not kno ...
... al., 1995; Morris et al., 1987; Town et al., 1976; Williams et al., 1987; Jermyn et al., 1987; Berks and Kay, 1990). The expression of prestalk-specific genes is mediated by a STAT transcription factor (Kawata et al., 1997); however, the role of DIF in potentially regulating STAT function is not kno ...
SUMO Pathway Modulation of Regulatory Protein Binding
... the cellular hub for the initial stages of ribosome assembly (Strunnikov et al. 2001; D’Amours et al. 2004; Panse et al. 2006; Takahashi et al. 2008; Srikumar et al. 2013b). To maintain robust ribosome production, cells have numerous ribosomal DNA (rDNA) copies, even though repeated DNA is prone to ...
... the cellular hub for the initial stages of ribosome assembly (Strunnikov et al. 2001; D’Amours et al. 2004; Panse et al. 2006; Takahashi et al. 2008; Srikumar et al. 2013b). To maintain robust ribosome production, cells have numerous ribosomal DNA (rDNA) copies, even though repeated DNA is prone to ...
An antagonist of integrin αvβ3 prevents maturation of blood vessels
... bright integrin αvβ3 immunofluorescence along the dorsal aorta (da). This LSCM image is constructed from multiple optical sections representing approximately 10 µm of vertical space. Staining is also associated with endodermal cells (asterisk). ×400. (B) Vitronectin is a constituent of the ECM adjac ...
... bright integrin αvβ3 immunofluorescence along the dorsal aorta (da). This LSCM image is constructed from multiple optical sections representing approximately 10 µm of vertical space. Staining is also associated with endodermal cells (asterisk). ×400. (B) Vitronectin is a constituent of the ECM adjac ...
PubMed Central CANADA
... and MTT was then added at a final concentration of 1mg/ml. After an additional 2 h incubation at 37 °C, culture media were carefully aspirated and 100 μl of dimethysulfoxide (DMSO) was added to dissolve crystals. Optical densities of the wells were analyzed using an automatic ELISA plate reader (Bio ...
... and MTT was then added at a final concentration of 1mg/ml. After an additional 2 h incubation at 37 °C, culture media were carefully aspirated and 100 μl of dimethysulfoxide (DMSO) was added to dissolve crystals. Optical densities of the wells were analyzed using an automatic ELISA plate reader (Bio ...
Merotelic kinetochore orientation occurs frequently during early
... by the injected proteins, we injected 19 metaphase PtK1 cells with Mad2∆C and found that none of them displayed lagging chromosomes at anaphase. We next tested for the incidence of merotelic orientation in prometaphase of cells recovering from a nocodazoleinduced mitotic block because of the higher ...
... by the injected proteins, we injected 19 metaphase PtK1 cells with Mad2∆C and found that none of them displayed lagging chromosomes at anaphase. We next tested for the incidence of merotelic orientation in prometaphase of cells recovering from a nocodazoleinduced mitotic block because of the higher ...
Chapter 4
... Microscopes as Windows on the World of Cells – Light microscopes can be used to explore the structures and functions of cells. – When scientists examine a specimen on a microscope slide • Light passes through the specimen • Lenses enlarge, or magnify, the image – Magnification is an increase in the ...
... Microscopes as Windows on the World of Cells – Light microscopes can be used to explore the structures and functions of cells. – When scientists examine a specimen on a microscope slide • Light passes through the specimen • Lenses enlarge, or magnify, the image – Magnification is an increase in the ...
Direct Comparison between T Cell Control of Hepatitis B Virus +CD8
... (15). Therefore, CD8+ T cell line and a clone specific for HBV core 18–27 epitope (HBc18–27) were generated and used in the current study. PBMCs cultured at 3 3 105/well in supplemented RPMI 1640/10% human AB serum, were stimulated with 10 mg/ml synthetic HBc18–27 (FLPSDFFPSV) (Cambridge Research Bi ...
... (15). Therefore, CD8+ T cell line and a clone specific for HBV core 18–27 epitope (HBc18–27) were generated and used in the current study. PBMCs cultured at 3 3 105/well in supplemented RPMI 1640/10% human AB serum, were stimulated with 10 mg/ml synthetic HBc18–27 (FLPSDFFPSV) (Cambridge Research Bi ...
Retinal Ganglion Cells in Goldfish
... The dendrites of a single subtype of ganglion cell (see below) were digitized using a Zeiss Universal microscope (2000x magnification), interfaced with a PDP 1l/34 computer through stepping motors on the mechanical controls of the stage and fine focus. Data points were entered sequentially, every 5- ...
... The dendrites of a single subtype of ganglion cell (see below) were digitized using a Zeiss Universal microscope (2000x magnification), interfaced with a PDP 1l/34 computer through stepping motors on the mechanical controls of the stage and fine focus. Data points were entered sequentially, every 5- ...
Transcripts of the npm-alk fusion gene in anaplastic large cell
... with the application of modem analytical techniques including immunohistochemistry, electron microscopy, and molecular analysis of T- and B-cell antigen receptor gene rearrangements. However, ALCL can still be confused with HD due to the presence of cells resembling Reed-Stemberg cells and an overla ...
... with the application of modem analytical techniques including immunohistochemistry, electron microscopy, and molecular analysis of T- and B-cell antigen receptor gene rearrangements. However, ALCL can still be confused with HD due to the presence of cells resembling Reed-Stemberg cells and an overla ...
Periodontal abscess
... •The entry of bacteria into the soft tissue pocket wall could be the event that initiates the formation of a periodontal abscess, however it is the accumulation of leukocytes and the formation of an acute inflammatory infiltrate what will be the main cause of the connective tissue destruction, enca ...
... •The entry of bacteria into the soft tissue pocket wall could be the event that initiates the formation of a periodontal abscess, however it is the accumulation of leukocytes and the formation of an acute inflammatory infiltrate what will be the main cause of the connective tissue destruction, enca ...
Cell shape changes indicate a role for extrinsic tensile forces in
... (‘total’ strain rates). Next we quantified the average rates of shape change for cells belonging to each domain (‘cell shape’ strain rates). Finally, the strain rates attributable to cell intercalation were derived for each domain by subtracting the ‘cell shape’ strain rates from the ‘total’ strain ...
... (‘total’ strain rates). Next we quantified the average rates of shape change for cells belonging to each domain (‘cell shape’ strain rates). Finally, the strain rates attributable to cell intercalation were derived for each domain by subtracting the ‘cell shape’ strain rates from the ‘total’ strain ...
1 - Purdue Department of Biological Sciences
... protoxylem, red bracket marks metaxylem. Bar=66 lm. c–j Cells numbered 1–4 show different developmental stages of xylem vessels, described in g. c In situ hybridization of ZeFLA11 sense riboprobe with an equivalent serial section as shown in b, showing nonspecific background labeling in stem bundle 12 ...
... protoxylem, red bracket marks metaxylem. Bar=66 lm. c–j Cells numbered 1–4 show different developmental stages of xylem vessels, described in g. c In situ hybridization of ZeFLA11 sense riboprobe with an equivalent serial section as shown in b, showing nonspecific background labeling in stem bundle 12 ...
Long-Term Potentiation in Cultures of Single Hippocampal Granule
... size and frequency of these events in the presence of tetrodotoxin (TTX) before and after inducing LTP. Figure 8A shows the results from one experiment. In basal conditions, the frequency of mEPSCs was approximately 0.2 Hz and the amplitude distribution showed a skewed distribution toward larger eve ...
... size and frequency of these events in the presence of tetrodotoxin (TTX) before and after inducing LTP. Figure 8A shows the results from one experiment. In basal conditions, the frequency of mEPSCs was approximately 0.2 Hz and the amplitude distribution showed a skewed distribution toward larger eve ...
A Model-Based Approach for Automated In Vitro Cell - LISA
... (typically 20 pixels) from the image border has been defined. The mark representing a cell position (i.e., a center c*) is frozen (i.e., its consecutive positions are invalidated) when it penetrates this border area. The second problem appears when two marks (that initially followed two different cel ...
... (typically 20 pixels) from the image border has been defined. The mark representing a cell position (i.e., a center c*) is frozen (i.e., its consecutive positions are invalidated) when it penetrates this border area. The second problem appears when two marks (that initially followed two different cel ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.