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... 8. Karpova, N. (2014). Role of BDNF epigenetics in activity-dependent neuronal plasticity. Neuropharmacology, 76: 709-718. 9. Cortes-Mendoza, J., et al. (2013). Shaping synaptic plasticity: the role of activity-mediated epigenetic regulation on gene transcription. International Journal of Developmen ...
... 8. Karpova, N. (2014). Role of BDNF epigenetics in activity-dependent neuronal plasticity. Neuropharmacology, 76: 709-718. 9. Cortes-Mendoza, J., et al. (2013). Shaping synaptic plasticity: the role of activity-mediated epigenetic regulation on gene transcription. International Journal of Developmen ...
Transcription Factor Veracity: 1s GBF3 Responsible for ABA
... GBF4, were isolated from Arabidopsis by using the tomato small unit of ribulose bisphosphate carboxylase (RbcS) G-box as the oligonucleotide probe for protein interaction screening and then by using GBFl as a hybridization probe (Schindler et al., 1992a; Menkens and Cashmore, 1994). The N-terminalpr ...
... GBF4, were isolated from Arabidopsis by using the tomato small unit of ribulose bisphosphate carboxylase (RbcS) G-box as the oligonucleotide probe for protein interaction screening and then by using GBFl as a hybridization probe (Schindler et al., 1992a; Menkens and Cashmore, 1994). The N-terminalpr ...
LIN-28 co-transcriptionally binds primary let
... MicroRNAs (miRNAs) function as ~22-nucleotide (nt) guide RNAs in the RNA-induced silencing complex (RISC) by binding to partially complementary sites in target mRNAs, causing inhibition of translation or destabilization. Let-7 miRNA that originally discovered in C. elegans is conserved across specie ...
... MicroRNAs (miRNAs) function as ~22-nucleotide (nt) guide RNAs in the RNA-induced silencing complex (RISC) by binding to partially complementary sites in target mRNAs, causing inhibition of translation or destabilization. Let-7 miRNA that originally discovered in C. elegans is conserved across specie ...
Functional and phylogenetic analyses of chromosome 21 promoters
... composition of expressed genes changes considerably among cell types and in response to physiological and environmental conditions [1-5]. Eukaryotic genomes contain on the order of 0.5–5x104 genes. To allow precise spacio-temporal gene expression, a particularly complex system of regulatory mechanis ...
... composition of expressed genes changes considerably among cell types and in response to physiological and environmental conditions [1-5]. Eukaryotic genomes contain on the order of 0.5–5x104 genes. To allow precise spacio-temporal gene expression, a particularly complex system of regulatory mechanis ...
Domains of the Adenovirus E1A Protein Required for
... Figure 1. Structure of E1A mutants. The wild-type 243-aminoacid product of the 12S E1A RNA. The two regions that exhibit sequence conservation among various adenovirus serotypes (Kimelman et al. 1985) are indicated. Point mutations are indicated by arrows; the name of the mutant indicates the amino ...
... Figure 1. Structure of E1A mutants. The wild-type 243-aminoacid product of the 12S E1A RNA. The two regions that exhibit sequence conservation among various adenovirus serotypes (Kimelman et al. 1985) are indicated. Point mutations are indicated by arrows; the name of the mutant indicates the amino ...
Transcription Factor Positive Regulatory Domain 4 (PRDM4) recruits Protein Arginine
... fate are likely to be governed by cell lineagespecific transcription factors acting in concert with epigenetic mechanisms (9-14). The latter include post-translational modifications of histones associated with regulatory elements of genes as well as DNA methylation at CpG dinucleotides, which togeth ...
... fate are likely to be governed by cell lineagespecific transcription factors acting in concert with epigenetic mechanisms (9-14). The latter include post-translational modifications of histones associated with regulatory elements of genes as well as DNA methylation at CpG dinucleotides, which togeth ...
Network Regulation of the Escherichia coli Maltose System
... activator which, together with the inducer maltotriose and ATP, is essential for mal gene transcription. Network regulation in this system affects the function of MalT and occurs on two levels. The first concerns the expression of malT. It has long been known that malT is under catabolite repression ...
... activator which, together with the inducer maltotriose and ATP, is essential for mal gene transcription. Network regulation in this system affects the function of MalT and occurs on two levels. The first concerns the expression of malT. It has long been known that malT is under catabolite repression ...
Inducible Gene Targeting in Postnatal Myocardium by Cardiac
... limitations, the possibility of somatic, tissue-specific recombination has gained appeal. One strategy for this exploits the Cre/loxP system derived from bacteriophage P1.2,3 The 38kDa Cre protein functions as a site-specific recombinase, splicing DNA between specific 34-bp sequences known as loxP s ...
... limitations, the possibility of somatic, tissue-specific recombination has gained appeal. One strategy for this exploits the Cre/loxP system derived from bacteriophage P1.2,3 The 38kDa Cre protein functions as a site-specific recombinase, splicing DNA between specific 34-bp sequences known as loxP s ...
The Second Exon-Encoded Factor XII Region Is Involved in the
... FXII activation by kallikrein. The kinetics of activation of FXII proteins by kallikrein were determined as described.14 Briefly, 25 µL of FXII (125 nmol/L) were added to 25 µL of dextran sulfate (DS500) (50 µg/mL) and 25 µL of buffer to yield final concentrations of 50 mmol/L Tris-HCl, 150 mmol/L N ...
... FXII activation by kallikrein. The kinetics of activation of FXII proteins by kallikrein were determined as described.14 Briefly, 25 µL of FXII (125 nmol/L) were added to 25 µL of dextran sulfate (DS500) (50 µg/mL) and 25 µL of buffer to yield final concentrations of 50 mmol/L Tris-HCl, 150 mmol/L N ...
Identification of a novel Endoplasmic Reticulum Stress Response
... similarity to the Endoplasmic Reticulum Stress Response Element (ERSE). This novel ERSE (“ERSE-26”) is able to regulate PRNP endogenously in response to endoplasmic reticulum (ER) stress. In order to determine whether or not the ERSE-26 exists elsewhere in the genome and what is co-regulated with PR ...
... similarity to the Endoplasmic Reticulum Stress Response Element (ERSE). This novel ERSE (“ERSE-26”) is able to regulate PRNP endogenously in response to endoplasmic reticulum (ER) stress. In order to determine whether or not the ERSE-26 exists elsewhere in the genome and what is co-regulated with PR ...
Transcription is the synthesis of RNA under the direction of DNA
... transcription, is the process of transcribing DNA nucleotide sequence information into RNA sequence information. Both nucleic acid sequences use complementary language, and the information is simply transcribed, or copied, from one molecule to the other. DNA sequence is enzymatically copied by RNA p ...
... transcription, is the process of transcribing DNA nucleotide sequence information into RNA sequence information. Both nucleic acid sequences use complementary language, and the information is simply transcribed, or copied, from one molecule to the other. DNA sequence is enzymatically copied by RNA p ...
Get PDF - Wiley Online Library
... (Tag et al., 2000). Because of the ubiquitous nature of the G-protein signalling pathway in higher organisms, it seems likely that A. nidulans has selected this pathway to link morphogenesis with secondary metabolism. The same mechanism can operate in other fungi that synthesize natural products at ...
... (Tag et al., 2000). Because of the ubiquitous nature of the G-protein signalling pathway in higher organisms, it seems likely that A. nidulans has selected this pathway to link morphogenesis with secondary metabolism. The same mechanism can operate in other fungi that synthesize natural products at ...
Modular evolution of phosphorylation
... indicates its importance in cellular function. The relative abundance of pSer/pThr-binding domains when compared with pTyr-recognition modules reflects the fact that the phosphorylation of serine/threonine sites is more ancient and more prevalent than tyrosine phosphorylation. A particular phosphope ...
... indicates its importance in cellular function. The relative abundance of pSer/pThr-binding domains when compared with pTyr-recognition modules reflects the fact that the phosphorylation of serine/threonine sites is more ancient and more prevalent than tyrosine phosphorylation. A particular phosphope ...
Ca Signaling11
... • Activity-dependent plasticity is mediated by electrochemical activity of the synapse. • Activity-dependent plasticity is a change in neural connections and synaptic strength that are the hallmarks of learning and memory. ...
... • Activity-dependent plasticity is mediated by electrochemical activity of the synapse. • Activity-dependent plasticity is a change in neural connections and synaptic strength that are the hallmarks of learning and memory. ...
Function of Conserved Tryptophans in the Aspergillus niger
... W590K mutation resulting in the abolition of binding at site 1 since W543 and W590 are both key binding residues at this site. By contrast, signals associated with binding site 2 shift by similar amounts in wild-type SBD and W590K (results not shown). Comparable results were obtained from W563K, sho ...
... W590K mutation resulting in the abolition of binding at site 1 since W543 and W590 are both key binding residues at this site. By contrast, signals associated with binding site 2 shift by similar amounts in wild-type SBD and W590K (results not shown). Comparable results were obtained from W563K, sho ...
Transcription-associated recombination in eukaryotes: link between
... stability by preventing the use of non-transcribed pseudogenes as donors during gene conversion (48). Overall, the available data in mammalian cells suggest that TAR and DSB-induced HR are mechanistically different processes with little influence on each other. Our recent data show that there is a d ...
... stability by preventing the use of non-transcribed pseudogenes as donors during gene conversion (48). Overall, the available data in mammalian cells suggest that TAR and DSB-induced HR are mechanistically different processes with little influence on each other. Our recent data show that there is a d ...
Ubiquitination and sumoylation of the HTLV-2 κB activity: a
... encompassing the four C-terminal amino acids responsible for the binding to several PDZ domain-containing proteins [16-18]. In addition, some HTLV-2 subtypes express shorter versions of Tax-2 (namely Tax-2A and Tax-2CG) which, contrary to Tax-2B, do not functionally inactivate p53 [10,19]. Recent st ...
... encompassing the four C-terminal amino acids responsible for the binding to several PDZ domain-containing proteins [16-18]. In addition, some HTLV-2 subtypes express shorter versions of Tax-2 (namely Tax-2A and Tax-2CG) which, contrary to Tax-2B, do not functionally inactivate p53 [10,19]. Recent st ...
ATP-binding-cassette (ABC) transport systems: Functional and
... and two GTP-binding proteins (p21ras , elongation factor Tu) [30], and that of adenylate kinase only [1], respectively. According to these predictions, the consensus fragment of all ABC domains (approx. 250 residues) is folded in an alternating series of six K-helical subdomains and ¢ve L-strands. T ...
... and two GTP-binding proteins (p21ras , elongation factor Tu) [30], and that of adenylate kinase only [1], respectively. According to these predictions, the consensus fragment of all ABC domains (approx. 250 residues) is folded in an alternating series of six K-helical subdomains and ¢ve L-strands. T ...
MEF2 responds to multiple calciumregulated signals in the control of
... Speci®cally, a sustained, tonic pattern of motor nerve ®ring directs myo®bers to the slow, oxidative myo®ber phenotype, while ®bers assume one of several fast ®ber phenotypes based on other patterns of neural activity. Shorter durations of tonic motor nerve activation associated with endurance exerc ...
... Speci®cally, a sustained, tonic pattern of motor nerve ®ring directs myo®bers to the slow, oxidative myo®ber phenotype, while ®bers assume one of several fast ®ber phenotypes based on other patterns of neural activity. Shorter durations of tonic motor nerve activation associated with endurance exerc ...
View PDF - Genetics
... The genetic screen used was based on the ability to monitor termination efficiency through the expression of ADE2 gene which encodes the P-ribosyl-amino-imidazole-carboxylase (EC 4.1.1.21), responsible for the degradation of the red pigment amino imidazole ribotide. The screen was performed in a FS1 ...
... The genetic screen used was based on the ability to monitor termination efficiency through the expression of ADE2 gene which encodes the P-ribosyl-amino-imidazole-carboxylase (EC 4.1.1.21), responsible for the degradation of the red pigment amino imidazole ribotide. The screen was performed in a FS1 ...
Crystal Structure of a 70 Subunit Fragment from
... used to identify specific amino acids important for core RNAP binding within E. coli s 54, an alternative s factor that is not a member of the s70 family (Wong et al., 1994; Tintut and Gralla, 1995). The largest concentration of mutations causing loss of core RNAP binding fell within or just adjacen ...
... used to identify specific amino acids important for core RNAP binding within E. coli s 54, an alternative s factor that is not a member of the s70 family (Wong et al., 1994; Tintut and Gralla, 1995). The largest concentration of mutations causing loss of core RNAP binding fell within or just adjacen ...
Clostridium difficile toxin synthesis is negatively regulated by TcdC
... an alternative s factor that mediates positive regulation of both the toxin genes and its own gene. The tcdA, tcdB and tcdR genes are transcribed during the stationary growth phase. The tcdC gene, however, is expressed during exponential phase. This expression pattern suggested that TcdC may act as ...
... an alternative s factor that mediates positive regulation of both the toxin genes and its own gene. The tcdA, tcdB and tcdR genes are transcribed during the stationary growth phase. The tcdC gene, however, is expressed during exponential phase. This expression pattern suggested that TcdC may act as ...
Cardiac O-GlcNAc signaling is increased in hypertrophy and heart
... Rat models and echocardiographic/hemodynamic evaluation. Experiments on rats were approved by the Norwegian National Animal Research Committee and conformed to the Guide for the Care and Use of Laboratory Animals [National Institutes of Health (NIH) publication no. 85-23, revised 1996, US]. Ligation ...
... Rat models and echocardiographic/hemodynamic evaluation. Experiments on rats were approved by the Norwegian National Animal Research Committee and conformed to the Guide for the Care and Use of Laboratory Animals [National Institutes of Health (NIH) publication no. 85-23, revised 1996, US]. Ligation ...
Gene expression profiling of Escherichia coli growth transitions: an
... Gene expression during transient growth arrest 293 seem to control gene expression under these conditions; these became our focus during the following analysis. Statistical and cluster analysis With this analysis, we aimed to identify those genes that appear to be involved directly or indirectly in ...
... Gene expression during transient growth arrest 293 seem to control gene expression under these conditions; these became our focus during the following analysis. Statistical and cluster analysis With this analysis, we aimed to identify those genes that appear to be involved directly or indirectly in ...
Histone acetylation and deacetylation
Histone acetylation and deacetylation are the processes by which the lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Histone acetylation and deacetylation are essential parts of gene regulation. These reactions are typically catalysed by enzymes with ""histone acetyltransferase"" (HAT) or ""histone deacetylase"" (HDAC) activity. Acetylation is the process where an acetyl functional group is transferred from one molecule (in this case, Acetyl-Coenzyme A) to another. Deacetylation is simply the reverse reaction where an acetyl group is removed from a molecule.Acetylated histones, octameric proteins that organize chromatin into nucleosomes and ultimately higher order structures, represent a type of epigenetic marker within chromatin. Acetylation removes the positive charge on the histones, thereby decreasing the interaction of the N termini of histones with the negatively charged phosphate groups of DNA. As a consequence, the condensed chromatin is transformed into a more relaxed structure that is associated with greater levels of gene transcription. This relaxation can be reversed by HDAC activity. Relaxed, transcriptionally active DNA is referred to as euchromatin. More condensed (tightly packed) DNA is referred to as heterochromatin. Condensation can be brought about by processes including deacetylation and methylation; the action of methylation is indirect and has no effect upon charge.