Microtubule-Associated Protein 1B
... LC1 Interacts with the CAMP Domain of EPAC1. We had previously demonstrated that the light chain of MAP1A (LC2) interacted with EPAC1 (Magiera et al., 2004). Given that LC2 is homologous to LC1 within the C-terminal segment of the protein (Noiges et al., 2002), we decided to check whether LC1 also i ...
... LC1 Interacts with the CAMP Domain of EPAC1. We had previously demonstrated that the light chain of MAP1A (LC2) interacted with EPAC1 (Magiera et al., 2004). Given that LC2 is homologous to LC1 within the C-terminal segment of the protein (Noiges et al., 2002), we decided to check whether LC1 also i ...
Materials - HAL
... FERM domains are commonly found within a family of peripheral membrane proteins that mediate linkage of the cytoskeleton to the plasma membrane (for review, Chishti et al., 1998; Bretscher, 1999; Mangeat at al., 1999). This family comprises the erythrocyte protein 4.1, ezrin, radixin, moesin, merli ...
... FERM domains are commonly found within a family of peripheral membrane proteins that mediate linkage of the cytoskeleton to the plasma membrane (for review, Chishti et al., 1998; Bretscher, 1999; Mangeat at al., 1999). This family comprises the erythrocyte protein 4.1, ezrin, radixin, moesin, merli ...
- Wiley Online Library
... dependences on [Ca2þ]i could be fitted with Hill equations, which yielded Kd and coefficients (n) of 32 3.2 mM and 4.4 and 23.7 2.5 mM and 7 for rapid and slow endocytosis, respectively (Figure1D). Typically, a Hill equation is used for chemical reactions whose detailed intermediate steps are no ...
... dependences on [Ca2þ]i could be fitted with Hill equations, which yielded Kd and coefficients (n) of 32 3.2 mM and 4.4 and 23.7 2.5 mM and 7 for rapid and slow endocytosis, respectively (Figure1D). Typically, a Hill equation is used for chemical reactions whose detailed intermediate steps are no ...
lee
... Two methods were used for observations of epithelial cells grown on filters. In the first, the filter cup was mounted directly into the chamber, and the fluorescence of Calu-3 cells was observed with one of the inverted microscopes through the filter with a long-working-distance (1.6-mm), waterimmer ...
... Two methods were used for observations of epithelial cells grown on filters. In the first, the filter cup was mounted directly into the chamber, and the fluorescence of Calu-3 cells was observed with one of the inverted microscopes through the filter with a long-working-distance (1.6-mm), waterimmer ...
Plasticity in Cell Division Patterns and Auxin
... histodifferentiation (Fan et al., 1988; Telmer et al., 1995; Ilic Grubor et al., 1998). These clusters increased in cell number during the culture period, forming unorganized masses of round, expanded cells, but eventually died. Our data suggest that many microspores are initially programmed to dev ...
... histodifferentiation (Fan et al., 1988; Telmer et al., 1995; Ilic Grubor et al., 1998). These clusters increased in cell number during the culture period, forming unorganized masses of round, expanded cells, but eventually died. Our data suggest that many microspores are initially programmed to dev ...
E-cadherin controls β-catenin and NF
... 480 cells the activity of the fibronectin and LEF1 promoters was upregulated by co-expression of VP16-Rel (supplementary material Fig. S4), a fusion chimera containing the Rel DNA-binding domain of NF-κB-p65 and the transactivator domain of VP-16. We checked with ChIP experiments whether binding of ...
... 480 cells the activity of the fibronectin and LEF1 promoters was upregulated by co-expression of VP16-Rel (supplementary material Fig. S4), a fusion chimera containing the Rel DNA-binding domain of NF-κB-p65 and the transactivator domain of VP-16. We checked with ChIP experiments whether binding of ...
Full-Text PDF
... columns. A number of various methods have been reported for the isolation and identification of sterols in fungi and higher plants. In most cases the extracts were first subjected to alkaline saponification before chromatographic analysis [24,25]. The saponification is most often accomplished by mea ...
... columns. A number of various methods have been reported for the isolation and identification of sterols in fungi and higher plants. In most cases the extracts were first subjected to alkaline saponification before chromatographic analysis [24,25]. The saponification is most often accomplished by mea ...
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... connexió immediata, per la complicitat i amistat sincera, a més de per la muntanya que encara hem de començar a fer junts. Thanks to my New York girls: Gracias a Carolina (la Pola), por ser la alegría de la huerta y por casi llevarme al huerto, además de por tu amistad. Gracias a Laura, todo-corazón ...
... connexió immediata, per la complicitat i amistat sincera, a més de per la muntanya que encara hem de començar a fer junts. Thanks to my New York girls: Gracias a Carolina (la Pola), por ser la alegría de la huerta y por casi llevarme al huerto, además de por tu amistad. Gracias a Laura, todo-corazón ...
the 5 kingdmcard 5 - Henrico County Public Schools
... invertebrates is that vertebrates have backbones, and invertebrates do not. SOL 5.5 ...
... invertebrates is that vertebrates have backbones, and invertebrates do not. SOL 5.5 ...
Suppressor analysis of the protein kinase Elm1p, an enzyme
... bud-site selection appear to be independent of one another, since specific mutations exist that interfere with one but not the other pathway. It is postulated that in axial budding, a labile marker is present at the previous site of budding, and thus nutrioit conditions that delay bud formation allo ...
... bud-site selection appear to be independent of one another, since specific mutations exist that interfere with one but not the other pathway. It is postulated that in axial budding, a labile marker is present at the previous site of budding, and thus nutrioit conditions that delay bud formation allo ...
Boundless Study Slides
... Free to share, print, make copies and changes. Get yours at www.boundless.com Molecular Cancer Journal. "Molecular Cancer | Full text | Role of APCand DNA mismatch repair genes in the development of colorectal cancers." CC BY http://www.molecular-cancer.com/content/2/1/41 View on Boundless.com ...
... Free to share, print, make copies and changes. Get yours at www.boundless.com Molecular Cancer Journal. "Molecular Cancer | Full text | Role of APCand DNA mismatch repair genes in the development of colorectal cancers." CC BY http://www.molecular-cancer.com/content/2/1/41 View on Boundless.com ...
The Evolutionary Biology of Hearing
... are, unlike in the papilla of Caiman, frequently difficult to distinguish in a surface view (Fig. 27.4). The hair cells are surrounded by supporting cells. T H C are the least specialized and most strongly resemble the typical hair cell of more primitive groups of vertebrates (Takasaka and Smith 197 ...
... are, unlike in the papilla of Caiman, frequently difficult to distinguish in a surface view (Fig. 27.4). The hair cells are surrounded by supporting cells. T H C are the least specialized and most strongly resemble the typical hair cell of more primitive groups of vertebrates (Takasaka and Smith 197 ...
Enzymes for Cell Dissociation and Lysis - Sigma
... The isoforms I and II of purified collagenase differ in their specificities and relative activities on native collagen and synthetic substrates. These two collagenases can be distinguished by their preference for one of the two different substrates used in SigmaAldrich’s assays. The Collagenase Dige ...
... The isoforms I and II of purified collagenase differ in their specificities and relative activities on native collagen and synthetic substrates. These two collagenases can be distinguished by their preference for one of the two different substrates used in SigmaAldrich’s assays. The Collagenase Dige ...
Enteropathogenic Escherichia coli (EPEC) adhesion to intestinal
... and effacing’ (A/E) lesions on small intestinal enterocytes. EPEC adhesins that have been associated with A/E adhesion and intestinal colonization include bundle-forming pili (BFP), EspA filaments and intimin. BFP are involved in bacteria–bacteria interaction and microcolony formation but their role ...
... and effacing’ (A/E) lesions on small intestinal enterocytes. EPEC adhesins that have been associated with A/E adhesion and intestinal colonization include bundle-forming pili (BFP), EspA filaments and intimin. BFP are involved in bacteria–bacteria interaction and microcolony formation but their role ...
The cell biology of lignification in higher plants
... Lignin (Latin lignum ‘wood’) is a polyphenolic polymer deposited directly in the cell wall of specialized cells. It is not only restricted to plant woody tissues but represents an integral feature ensuring the proper cellular function of many other cell types in different tissues/organs of the plant ...
... Lignin (Latin lignum ‘wood’) is a polyphenolic polymer deposited directly in the cell wall of specialized cells. It is not only restricted to plant woody tissues but represents an integral feature ensuring the proper cellular function of many other cell types in different tissues/organs of the plant ...
Gram`s staining - Micro-Rao
... cell wall active agents such as lysozyme or antibiotics may become Gram negative. Gram-positive bacteria such Actinomyces, Arthobacter, Corynebacterium, Mycobacterium, and Propionibacterium have cell walls particularly sensitive to breakage during cell division, resulting in Gram-negative staining o ...
... cell wall active agents such as lysozyme or antibiotics may become Gram negative. Gram-positive bacteria such Actinomyces, Arthobacter, Corynebacterium, Mycobacterium, and Propionibacterium have cell walls particularly sensitive to breakage during cell division, resulting in Gram-negative staining o ...
Transcription Factor Positive Regulatory Domain 4 (PRDM4) recruits Protein Arginine
... (2,3). This suggests that the temporal program of NSC division and cell fate specification is cell-intrinsic, but the molecular nature of the program is not known. The transitions in NSC fate are likely to be governed by cell lineagespecific transcription factors acting in concert with epigenetic me ...
... (2,3). This suggests that the temporal program of NSC division and cell fate specification is cell-intrinsic, but the molecular nature of the program is not known. The transitions in NSC fate are likely to be governed by cell lineagespecific transcription factors acting in concert with epigenetic me ...
14-3-3 associates with cell surface aminopeptidase N in the
... APN (GenBank accession number: NM_001150). Fig. 4A shows the inhibitory effect of siRNA on APN expression, as detected by immunofluorescence analysis. The quantification of the APN fluorescence intensity, which was normalized against the number of nuclei in each field (DAPI stain), revealed 50% and ...
... APN (GenBank accession number: NM_001150). Fig. 4A shows the inhibitory effect of siRNA on APN expression, as detected by immunofluorescence analysis. The quantification of the APN fluorescence intensity, which was normalized against the number of nuclei in each field (DAPI stain), revealed 50% and ...
Pathogenicity and Commensalism Recognition Contribute to Fungal
... touching the spore cell wall. Zymolyase was inactivated by heat (65˚C for 2 min) and washed away carefully, together with the remainder of the empty ascus; the spores were then resuspended to a concentration of 108 cells/ml. The zymolyase treatment did not affect spore cell wall composition, and spo ...
... touching the spore cell wall. Zymolyase was inactivated by heat (65˚C for 2 min) and washed away carefully, together with the remainder of the empty ascus; the spores were then resuspended to a concentration of 108 cells/ml. The zymolyase treatment did not affect spore cell wall composition, and spo ...
(ECM) Binding Sites on the Basal Surface of Embryonic Corneal
... extends cell processes called blebs (4, 20). On collagenous substrata, the basal surface flattens, whereas it continues to bleb on bare Millipore filters. The epithelium cultured on lens capsule or collagen gel produces over twice as much collagen as does comparable epithelium grown on noncollagenou ...
... extends cell processes called blebs (4, 20). On collagenous substrata, the basal surface flattens, whereas it continues to bleb on bare Millipore filters. The epithelium cultured on lens capsule or collagen gel produces over twice as much collagen as does comparable epithelium grown on noncollagenou ...
D. Malpighian tubules play similar function as tubular parts of the
... and human kidneys, the genetic tools available in Drosophila can be utilized to understand the kidney development and associated disorder. Since Malpighian tubules develop from ectodermal and mesodermal cell lineages, it provides opportunity to identify novel genes involved in the integration of cel ...
... and human kidneys, the genetic tools available in Drosophila can be utilized to understand the kidney development and associated disorder. Since Malpighian tubules develop from ectodermal and mesodermal cell lineages, it provides opportunity to identify novel genes involved in the integration of cel ...
UNIT 1 – FORCE AND MOTION (SEPUP Force and
... B-2.1 Recall the three major tenets of cell theory (all living things are composed of one or more cells; cells are the basic units of structure and function in living things; and all presently existing cells arose from previously existing cells). B-2.2 Summarize the structures and functions of organ ...
... B-2.1 Recall the three major tenets of cell theory (all living things are composed of one or more cells; cells are the basic units of structure and function in living things; and all presently existing cells arose from previously existing cells). B-2.2 Summarize the structures and functions of organ ...
An Early Tobacco Mosaic Virus-Induced Oxidative Burst in Tobacco
... defense gene expression, and frequently hypersensitive response (HR). ROS induction by fungal or bacterial elicitors often occurs in two distinct phases. Very rapid responses (within minutes or hours), termed phase I, are not always correlated with plant disease resistance, whereas later ROS product ...
... defense gene expression, and frequently hypersensitive response (HR). ROS induction by fungal or bacterial elicitors often occurs in two distinct phases. Very rapid responses (within minutes or hours), termed phase I, are not always correlated with plant disease resistance, whereas later ROS product ...
Full Text - Molecular Cancer Research
... of ATM-null embryos, and simultaneous deletion of ATM and other DNA damage repair factors including histone H2AX and DNA-dependent protein kinase is embryonic lethal in mice (12, 13). However, owing to the relative inaccessibility of mammalian embryonic development, it is unclear what roles ATM may ...
... of ATM-null embryos, and simultaneous deletion of ATM and other DNA damage repair factors including histone H2AX and DNA-dependent protein kinase is embryonic lethal in mice (12, 13). However, owing to the relative inaccessibility of mammalian embryonic development, it is unclear what roles ATM may ...
Bacterial toxins modifying the actin cytoskeleton
... which is closely related to ToxB (88% amino acid sequence identity and immunological cross reactions between the two toxins) and a hemorrhagic toxin (HT). C. novyi is an etiological agent of gangrene with major edema. The main toxin produced is the alpha toxin. The large clostridial toxins have a co ...
... which is closely related to ToxB (88% amino acid sequence identity and immunological cross reactions between the two toxins) and a hemorrhagic toxin (HT). C. novyi is an etiological agent of gangrene with major edema. The main toxin produced is the alpha toxin. The large clostridial toxins have a co ...
Mitosis
Mitosis is a part of the cell cycle in which chromosomes in a cell nucleus are separated into two identical sets of chromosomes, each in its own nucleus. In general, mitosis (division of the nucleus) is often followed by cytokinesis, which divides the cytoplasm, organelles and cell membrane into two new cells containing roughly equal shares of these cellular components. Mitosis and cytokinesis together define the mitotic (M) phase of an animal cell cycle—the division of the mother cell into two daughter cells, genetically identical to each other and to their parent cell.The process of mitosis is divided into stages corresponding to the completion of one set of activities and the start of the next. These stages are prophase, prometaphase, metaphase, anaphase, and telophase. During mitosis, the chromosomes, which have already duplicated, condense and attach to fibers that pull one copy of each chromosome to opposite sides of the cell. The result is two genetically identical daughter nuclei. The cell may then divide by cytokinesis to produce two daughter cells. Producing three or more daughter cells instead of normal two is a mitotic error called tripolar mitosis or multipolar mitosis (direct cell triplication / multiplication). Other errors during mitosis can induce apoptosis (programmed cell death) or cause mutations. Certain types of cancer can arise from such mutations.Mitosis occurs only in eukaryotic cells and the process varies in different organisms. For example, animals undergo an ""open"" mitosis, where the nuclear envelope breaks down before the chromosomes separate, while fungi undergo a ""closed"" mitosis, where chromosomes divide within an intact cell nucleus. Furthermore, most animal cells undergo a shape change, known as mitotic cell rounding, to adopt a near spherical morphology at the start of mitosis. Prokaryotic cells, which lack a nucleus, divide by a different process called binary fission.