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The genetic engineers toolkit
The genetic engineers toolkit

... • This can take a small piece of DNA and copy it so you have lots (amplification)and it only takes a few hours • It is divides into 3 stages *Denaturing the DNA Heating it up to separate the strands * Annealing- attaching a primer to the strand. * Extension- copying the template strand. Taq polymera ...
Notes Unit 4 Part 7
Notes Unit 4 Part 7

... 3. Each codon has a complementary ______________ which is found on tRNA. For every codon read, tRNA attaches the anticodon.  anticodon = complementary base sequence to the __________ codon 4. Attached to the other end of the ___________ is an _____________ acid. When tRNA binds to mRNA, amino acids ...
Hotstart Taq DNA Polymerase
Hotstart Taq DNA Polymerase

... 10X reaction buffer: Buffer containing 25 mM MgCl2 ...
DNA Technology
DNA Technology

...  To diagnose diseases. Ex. HIV, cystic fibrosis, hemophilia, cancer, flu  To trace ancestry There are many examples p. 416-422 of text to give you ideas, but do not use your text to write the report – just to give you ideas. Report for Second Quarter: For each of these TWO technologies (Gene splic ...
Living Things are Mostly Made of*.
Living Things are Mostly Made of*.

DNA
DNA

... Structure of DNA • These 4 nucleotides are linked in various ways and order – creating DNA. • There are 2 strands of DNA • The sugar and the phosphate make up the backbone of the DNA. • One nucleotide from each strand come bond together to form what looks like ...
Quiz 3 review sheet
Quiz 3 review sheet

... Quiz 3 Learning Goals: use to review for Quiz 3 Replication, Transcription, Translation • Demonstrate how the structure of DNA, including its directionality and its double-stranded base pairing, are critical for its functions. • Explain the “central dogma” and how it relates to how genes determine p ...
Chpt. 10- Molecular Biology of the Gene - TJ
Chpt. 10- Molecular Biology of the Gene - TJ

... b. Chain separated by the enzyme helicase 1. What bonds are broken by helicase as it moves up the DNA chain? 2. Origin of replication a. DNA sequence that signifies where helicase will attach and start replication 2. The enzyme DNA polymerase binds to separated chains of DNA a. Brings new nucleotide ...
Chapter 10 DNA Replication and Expression
Chapter 10 DNA Replication and Expression

semester 1 review
semester 1 review

... 35. Identify the steps in cellular respiration in the proper sequence. For each indicate where it occurs (be specific). Indicate the products of each step (include the electron carriers) 36. How are cellular respiration and photosynthesis almost opposite processes? 37. What are the 2 types of fermen ...
DNA Replication
DNA Replication

... A.1. Abilities necessary to do scientific inquiry B.2. Structures and properties of matter C.1.c. Cells store and use information to guide their functions C.1.d. Cell functions are regulated C1. f. Cells can differentiate, and complex multi-cellular organisms are formed as a highly organized arrange ...
2 notes cp u8
2 notes cp u8

... What is the function rRNA? – Helps to bond amino acids together in protein synthesis  What is the function tRNA? – tRNA brings amino acids to the ribosomal RNA so they can be assembled into proteins – Has the anticodon that matches with the codon on the mRNA ...
Biology and you - properties of life and the scientific method
Biology and you - properties of life and the scientific method

... 2. Adult Stem Cells- (aka-Somatic Stem Cells) cells associated with a certain organ or organ system. What is DNA replication? ...
Nelson Bio 12 Ch. 4 – DNA : The Molecular Basis of Life
Nelson Bio 12 Ch. 4 – DNA : The Molecular Basis of Life

amp R - Fort Bend ISD
amp R - Fort Bend ISD

... Overview of How Bacterial Plasmids Are Used to Clone Genes ...
DNA Structure Cornell Notes
DNA Structure Cornell Notes

... enzymes are proteins) which in turn control many of the biochemical reactions in your body. So in a way the sequence of nitrogen bases controls everything. ...
Getting to know DNA - noraddin
Getting to know DNA - noraddin

... the process by which perfect copies of our DNA are made! ...
ch 20 study guide: dna technology
ch 20 study guide: dna technology

... Liga - = bound, tied (DNA ligase: a linking enzyme essential for DNA replication) Electro - = electricity (electroporation: a technique to introduce recombinant DNA into cells by applying a breif electrical pulse to a solution containing cells) Poly - = many; morph - = form (Single nucleotide polymo ...
Which of the following organisms are autotrophs? algae
Which of the following organisms are autotrophs? algae

... cleavage ...
Nucleotides and DNA Structure
Nucleotides and DNA Structure

... • Inaccuracy of two‐ dimensional  drawing:  bases  are perpendicular  to the paper • “ladder” • H‐bonding ...
Slide 1
Slide 1

...  When DNA strands are separated in the PCR, what type of bond is broken?  It is important in the PCR that the fragments of DNA used are not contaminated with any other biological material. Suggest a reason why. ...
PDF - Qompendium
PDF - Qompendium

... may occasionally be encountered in pre-1953 genetics. ...
(DNA and RNA).
(DNA and RNA).

... HAPLOID: A cell containing a set of single chromosomes, or an organism composed of haploid cells. Gametes are haploid cells. HEARTBREAKERS: The selfchosen name of Sorenson’s Betaseries clones, engineered as bodyguards. Except for Queenie, the original Heartbreakers are dead; the current group was cr ...
Ch08
Ch08

... 1. This is not a trivial question! Check out Fig 8-11 which shows the H-bonding between A-T and G-C base pairs. Those “Watson-Crick” H-bonds that are part of holding the two DNA strands together which along with hydrophobic stacking allows DNA to form stable double stranded structure. But consider t ...
Biology Assessment #3:
Biology Assessment #3:

... 2. Compare and contrast DNA and RNA (location, function, size/structure/shape, nitrogen bases, etc.) 3. Explain how protein synthesis occurs. Explain the steps of transcription/translation. What is the role of different the types of RNA and of DNA in protein synthesis? 4. How are amino acids coded f ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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