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university of oslo
university of oslo

... Baseless site: base excision repair (pages 526-527). c) Describe the short patch nucleotide excision repair system of E. coli. (pages 527-528) !. UvrAB complex binds to damaged nucleotide. 2. UvrA leaves the complex and UvrC attaches. 3. DNA is cut by UvrB and UvrC, and segment (usually 12 nucleotid ...
7B Warm-Up Monday, November 10, 2008 #25
7B Warm-Up Monday, November 10, 2008 #25

... After a recent serious illness, Neh Neh had some blood work done. It was discovered that her T-cell or lymphocyte count was low. T-cells are the cells in blood that allow your immune system to fight off infection. In seven samples of blood there are 168 Tcells. This is after a total of 7 cells were ...
Exam V2002 - English
Exam V2002 - English

... Baseless site: base excision repair (pages 526-527). c) Describe the short patch nucleotide excision repair system of E. coli. (pages 527-528) !. UvrAB complex binds to damaged nucleotide. 2. UvrA leaves the complex and UvrC attaches. 3. DNA is cut by UvrB and UvrC, and segment (usually 12 nucleotid ...
2421 _Ch8.ppt
2421 _Ch8.ppt

... RNA Transcription RNA polymerase transcribes mRNA using the DNA template (the "coding" strand of the double-stranded DNA) the new RNA strand has ribonucleotides instead of deoxyribonucleotides & uracil (U) is used in place of thymine (T) to base pair with adenine (A) RNA polymerase binds to a promo ...
GENETIC Material
GENETIC Material

12 Week CCA Test Review
12 Week CCA Test Review

... 16. Look at the above diagram; the sex cells have how many chromosomes compared to the parent cell? 17. The purpose of meiosis is to take a parent cell (2n) and use it to create ______________cells with half the number of chromosomes (n) as the original cell. 18. What is a homologous chromosome and ...
Chapter 17_part 2
Chapter 17_part 2

... of the double helix which can occur at either end or in the middle. Special unwinding proteins called helicases, attach themselves to one DNA strand and cause the separation of the double helix. ...
DNA replication - Olympic High School
DNA replication - Olympic High School

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Genes, Chromosomes, and DNA

... 1. DNA is found in all living things and carries the instructions to make proteins – A single DNA strand holds the information to build many different proteins ...
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DNA Replication

... Enzymes in DNA replication • Enzymes are proteins that act as machines in the cell to do work. • Many enzymes come together to form a multi-functional replication machine called a Replisome at the replication fork. ...
How the form and function of DNA relate
How the form and function of DNA relate

... • Oswald Avery (1944, Canadian) used enzymes to break down the primary components (lipids, nucleic acids, proteins, carbohydrates) of the bacteria cells in Griffith’s experiment to ...
APDNA 2015 16
APDNA 2015 16

... Hershey and Chase (1952): DNA is the genetic material Erwin Chargaff (1947): The amount of thymine = adenine Watson and Crick (1953): Structure of DNA Rosalind Franklin (1951): X-Ray Structure of DNA Meselson and Stahl (1958): DNA Replication ...
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DNA

... S, the mouse gets pneumonia. ...
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DNA Scavenger Hunt

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DNA Structure Copy Cats Protein Nucleic Acids RANDOM!

review sheet
review sheet

... a. Label the diagram with the following terms: hydrogen bond, phosphodiester bond, deoxyribose, phosphate group. (Use each term only once) b. Label ALL the nitrogenous bases present with the appropriate letter (A,C,G,T). c. What part of the DNA molecule actually contains the hereditary information? ...
Restriction Mapping Restriction Fragment Length Polymorphism
Restriction Mapping Restriction Fragment Length Polymorphism

... by one enzyme contains restriction sites for the other enzyme. Fragments are aligned by size. ...
DNA Transcription / Translation
DNA Transcription / Translation

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1. Gene Mutations

... (1953): using all the research to date, discovered the structure for DNA: A DOUBLE HELIX (with sugar-phosphate backbones and bases on the inside held together by H bonds) ...
DNA/RNA/Protein Synthesis Study Guide
DNA/RNA/Protein Synthesis Study Guide

... carries the DNA message from the (A)_____________ to the _______________. There, the (G) _________ attaches to the surface of (C) ___________, which is made partly of the second type of RNA, ___________. Thirdly, Structure D, also known as _________, is responsible for carrying both the (E) ________ ...
File
File

... Okazaki fragment - Small pieces of DNA which form during DNA replication since DNA polymerase must work backwards on some strands. The Okazaki fragments are joined together by the enzyme DNA ligase. point mutation - A mutation which occurs when one base in the DNA molecule is replaced by another. Th ...
Changes in signal transduction pathways can alter
Changes in signal transduction pathways can alter

... processes that increase genetic variation. • The imperfect nature of DNA replication and repair increase variation (Mutation) • Transformation, transduction, conjugation, and ...
DNA replication machinery
DNA replication machinery

... action of helicase, which breaks the hydrogen bonds holding the two DNA strands together. The resulting structure has two branching "prongs", each one made up of a single strand of DNA. Leading strand synthesis In DNA replication, the leading strand is defined as the new DNA strand at the replicatio ...
Bononformatics
Bononformatics

... Genes are the “program” that every living thing uses to recreate itself. Genes control everything about our bodies, such as the length of our bones or the color of our hair. The real difference between a human and a tree is the structure of the genes of the two living organisms. Since the mapping of ...
nitrogenous base - Brookwood High School
nitrogenous base - Brookwood High School

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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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