The cell-cycle interactome: a source of growth
... been characterized for their effects on plant growth. When CYCD2;1 is overexpressed in Arabidopsis, plants grow to a size comparable to wild-type plants (Zhou et al., 2003; Qi and John, 2007). However, the leaves contain more cells that are smaller and have a decrease in DNA content (Qi and John, 20 ...
... been characterized for their effects on plant growth. When CYCD2;1 is overexpressed in Arabidopsis, plants grow to a size comparable to wild-type plants (Zhou et al., 2003; Qi and John, 2007). However, the leaves contain more cells that are smaller and have a decrease in DNA content (Qi and John, 20 ...
Genetic or epigenetic difference causing discordance between
... Epigenetics is defined as the study of mitotically or meiotically heritable variations in gene function that ...
... Epigenetics is defined as the study of mitotically or meiotically heritable variations in gene function that ...
Three epigenetic information channels and their different roles in
... classified together mechanistically – for example as being based on DNA methylation – will have quite different evolutionary consequences depending upon the way in which they are deployed. Amongst those transgenerational epigenetic effects that are adaptive and have been selected, we distinguish bet ...
... classified together mechanistically – for example as being based on DNA methylation – will have quite different evolutionary consequences depending upon the way in which they are deployed. Amongst those transgenerational epigenetic effects that are adaptive and have been selected, we distinguish bet ...
Impact of genetic engineering on the understanding of
... by endogenous regulatory sequence of the gene of interest (socalled knock-in). The latter enables the study of gene complementation, such as a possible rescue of a disease by a member of the same gene family. Gene-trap mutagenesis in embryonic stem cells (ES) is a powerful forward genetic approach i ...
... by endogenous regulatory sequence of the gene of interest (socalled knock-in). The latter enables the study of gene complementation, such as a possible rescue of a disease by a member of the same gene family. Gene-trap mutagenesis in embryonic stem cells (ES) is a powerful forward genetic approach i ...
Driscoll Katee Driscoll Dr. Ely Genetics October 20, 2013 Effects of
... Optimization procedures were applied to the models in order to produce the optimal prediction model, which relied on the method of splitting the data (using some of the data as a training set to predict the HTT CAG repeat lengths for the remaining samples that comprised the other set) (Lee et al. 20 ...
... Optimization procedures were applied to the models in order to produce the optimal prediction model, which relied on the method of splitting the data (using some of the data as a training set to predict the HTT CAG repeat lengths for the remaining samples that comprised the other set) (Lee et al. 20 ...
View PDF - BioEnergy Science Center
... compared to their controls. The stem density was significantly increased in at least two independent WRKY-DR lines in the WT, COMT-antisense and HCT-antisense backgrounds (Figure 4a). Overall biomass production was also improved by down-regulation of the WRKY gene in the WT, COMT-antisense and HCT-a ...
... compared to their controls. The stem density was significantly increased in at least two independent WRKY-DR lines in the WT, COMT-antisense and HCT-antisense backgrounds (Figure 4a). Overall biomass production was also improved by down-regulation of the WRKY gene in the WT, COMT-antisense and HCT-a ...
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... H2AX organization around the tss in the replicating genome is noteworthy. The canonical nucleosome and the H2AZ variant typically display the arrangement of −1 nucleosome and nucleosome-free region (NFR) upstream of the tss, and +1 nucleosome stably residing just downstream of the tss (17,25) (Suppl ...
... H2AX organization around the tss in the replicating genome is noteworthy. The canonical nucleosome and the H2AZ variant typically display the arrangement of −1 nucleosome and nucleosome-free region (NFR) upstream of the tss, and +1 nucleosome stably residing just downstream of the tss (17,25) (Suppl ...
Lluís Millán Ariño GENOMIC DISTRIBUTION AND FUNCTIONAL SPECIFICITY OF
... anti-HA antibody. The abundance of IPed material was quantified by qPCR with specific oligonucleotides for the MMTV promoter (nucleosome B). (B and C) The H1 valley at TSS of JUN and FOS genes is preformed and increases upon mitogenic stimulation. T47D cells were treated with PMA 100nM for 60 min or ...
... anti-HA antibody. The abundance of IPed material was quantified by qPCR with specific oligonucleotides for the MMTV promoter (nucleosome B). (B and C) The H1 valley at TSS of JUN and FOS genes is preformed and increases upon mitogenic stimulation. T47D cells were treated with PMA 100nM for 60 min or ...
Characterization of Chicken MMP13 Expression and Genetic Effect
... deletion promoter reporters, five forward primers (from pGL3MMP13-1F to pGL3-MMP13-5F, Table 1), and one reverse primer (pGL3-MMP13-R, Table 1) located downstream of the transcription start site of chicken MMP13 gene were synthesized. All forward primers contained an MluI site at their 59-ends, and t ...
... deletion promoter reporters, five forward primers (from pGL3MMP13-1F to pGL3-MMP13-5F, Table 1), and one reverse primer (pGL3-MMP13-R, Table 1) located downstream of the transcription start site of chicken MMP13 gene were synthesized. All forward primers contained an MluI site at their 59-ends, and t ...
Xdbx inhibits neurogenesis - Development
... neural progenitor domains in the embryo? The homeodomaincontaining gene Xiro3 is expressed at highest levels in neural progenitors and when overexpressed, Xiro3 inhibits neuronal differentiation in the embryo (Bellefroid et al., 1998). The Xenopus zinc finger transcription factor gene Zic2 may also ...
... neural progenitor domains in the embryo? The homeodomaincontaining gene Xiro3 is expressed at highest levels in neural progenitors and when overexpressed, Xiro3 inhibits neuronal differentiation in the embryo (Bellefroid et al., 1998). The Xenopus zinc finger transcription factor gene Zic2 may also ...
DNA Sequence Changes of Mutations Altering
... When double mutants were prepared with his01242 and either the ochre (his09654) or the UGA (his09675) mutations, both of which block leader peptide gene translation, or with one of the mutations altering the BC stem (h&09663), one class of recombinant5 yielded only rough transductants in the backcro ...
... When double mutants were prepared with his01242 and either the ochre (his09654) or the UGA (his09675) mutations, both of which block leader peptide gene translation, or with one of the mutations altering the BC stem (h&09663), one class of recombinant5 yielded only rough transductants in the backcro ...
GATA factor function in heart development
... pnr gene affects heart development. (A-D) Expression of pnr results in overexpression of a D-mef2 heart enhancer and cardiac hyperplasia. Stage 11 (A,B) and 16 (C,D) embryos stained for lacZ expression under the control of a D-mef2 cardial cell enhancer are shown. (A,C) WT, wild-type embryos; (B,D) ...
... pnr gene affects heart development. (A-D) Expression of pnr results in overexpression of a D-mef2 heart enhancer and cardiac hyperplasia. Stage 11 (A,B) and 16 (C,D) embryos stained for lacZ expression under the control of a D-mef2 cardial cell enhancer are shown. (A,C) WT, wild-type embryos; (B,D) ...
PDF
... supplementary material Fig. S2E). Knock down of either CDK-9 or cyclin T caused defective gastrulation and early embryonic arrest at ~120 cells (supplementary material Fig. S3). These phenotypes correlate with defective somatic zygotic transcription and also occur with depletion of the large subunit ...
... supplementary material Fig. S2E). Knock down of either CDK-9 or cyclin T caused defective gastrulation and early embryonic arrest at ~120 cells (supplementary material Fig. S3). These phenotypes correlate with defective somatic zygotic transcription and also occur with depletion of the large subunit ...
S2 File.
... Fig A: Results of optical density measurement (top) and the crystal violet assay (bottom) for replicates (n = 3) of the wells that were harvested for RNA sequencing. Wells without NaCl addition were harvested after 1 day of incubation, while wells with NaCl addition were harvested after 2 days, to ...
... Fig A: Results of optical density measurement (top) and the crystal violet assay (bottom) for replicates (n = 3) of the wells that were harvested for RNA sequencing. Wells without NaCl addition were harvested after 1 day of incubation, while wells with NaCl addition were harvested after 2 days, to ...
Replication timing as an epigenetic mark
... a few dozen individual gene loci using molecular approaches, with the finding that early replicating genes could be either expressed or silent, while late replicating genes were almost always silent, leading to the hypothesis that early replication is necessary for transcriptional competence but is ...
... a few dozen individual gene loci using molecular approaches, with the finding that early replicating genes could be either expressed or silent, while late replicating genes were almost always silent, leading to the hypothesis that early replication is necessary for transcriptional competence but is ...
SABRE is required for stabilization of root hair
... to pathways of hormone biosynthesis and signal transduction (Schiefelbein 2000, Grierson et al. 2001, Guimil and Dunand 2006). Ethylene and auxin have long been known to affect the development of root hairs (Masucci and Schiefelbein 1994, Masucci and Schiefelbein 1996) and, so far, all studies have ...
... to pathways of hormone biosynthesis and signal transduction (Schiefelbein 2000, Grierson et al. 2001, Guimil and Dunand 2006). Ethylene and auxin have long been known to affect the development of root hairs (Masucci and Schiefelbein 1994, Masucci and Schiefelbein 1996) and, so far, all studies have ...
CfE Higher Biology Unit 1: DNA and the Genome
... explain that the two strands of DNA lie anti-parallel to each other and are read in different directions; ...
... explain that the two strands of DNA lie anti-parallel to each other and are read in different directions; ...
64$ CfE Higher Biology Unit 1: DNA and the
... called base pairing; secondly, that DNA sequences vary between species. In the early 1950s, work by Maurice Wilkins and Rosalind Franklin uncovered some characteristic features of the DNA molecule. Using a method called X-ray crystallography, it was shown that the molecule had a helical structure. U ...
... called base pairing; secondly, that DNA sequences vary between species. In the early 1950s, work by Maurice Wilkins and Rosalind Franklin uncovered some characteristic features of the DNA molecule. Using a method called X-ray crystallography, it was shown that the molecule had a helical structure. U ...
64$ CfE Higher Biology Unit 1: DNA and the
... called base pairing; secondly, that DNA sequences vary between species. In the early 1950s, work by Maurice Wilkins and Rosalind Franklin uncovered some characteristic features of the DNA molecule. Using a method called X-ray crystallography, it was shown that the molecule had a helical structure. U ...
... called base pairing; secondly, that DNA sequences vary between species. In the early 1950s, work by Maurice Wilkins and Rosalind Franklin uncovered some characteristic features of the DNA molecule. Using a method called X-ray crystallography, it was shown that the molecule had a helical structure. U ...
Chapter 20
... dideoxyribonucleotides (ddNTP) attach to synthesized DNA strands of different lengths • Each type of ddNTP is tagged with a distinct fluorescent label that identifies the nucleotide at the end of each DNA fragment • The DNA sequence can be read from the ...
... dideoxyribonucleotides (ddNTP) attach to synthesized DNA strands of different lengths • Each type of ddNTP is tagged with a distinct fluorescent label that identifies the nucleotide at the end of each DNA fragment • The DNA sequence can be read from the ...
Roots Redefined: Anatomical and Cenetic Analysis of Root
... root and they subsequently acquired root cap characteristics. Upon ablation of cortical initials, pericycle initial cells expanded, divided into the resulting cavity, and subsequently formed cortical parenchyma and endodermis. These and related experiments show that positional information is importa ...
... root and they subsequently acquired root cap characteristics. Upon ablation of cortical initials, pericycle initial cells expanded, divided into the resulting cavity, and subsequently formed cortical parenchyma and endodermis. These and related experiments show that positional information is importa ...
Boundary elements and nuclear organization
... 54 kb region by ChIP (chromatin immunoprecipitation) analysis has shown that the HS4 insulator sequence maps to the transition point between the heterochromatic block, constitutively enriched in methylated H3 K9, and the active region, marked by hyperacetylation of H3 and H4, upon developmental indu ...
... 54 kb region by ChIP (chromatin immunoprecipitation) analysis has shown that the HS4 insulator sequence maps to the transition point between the heterochromatic block, constitutively enriched in methylated H3 K9, and the active region, marked by hyperacetylation of H3 and H4, upon developmental indu ...
NEM1 acts as a suppressor of apoptotic phenotypes in LSM4 yeast
... gene isolated as a suppressor in the same screening. NEM1 encodes the homolog of the human Dullard protein, a specific phosphatase that catalyzes lipin Oγ -phosphoryl serines (pSer) hydrolysis and negatively regulates bone morphogenetic protein (BMP) signaling (Satow et al. 2006). Alignment of human ...
... gene isolated as a suppressor in the same screening. NEM1 encodes the homolog of the human Dullard protein, a specific phosphatase that catalyzes lipin Oγ -phosphoryl serines (pSer) hydrolysis and negatively regulates bone morphogenetic protein (BMP) signaling (Satow et al. 2006). Alignment of human ...
hematopoietic stem cells: to be or notch to be
... functions, its participation diverges among the different organs and tissues. For example, in the mammalian hematopoietic system Notch1 is required for the generation of hematopoietic stem cells in the embryo but it is dispensable for the maintenance of the hematopoietic stem cells in the adult orga ...
... functions, its participation diverges among the different organs and tissues. For example, in the mammalian hematopoietic system Notch1 is required for the generation of hematopoietic stem cells in the embryo but it is dispensable for the maintenance of the hematopoietic stem cells in the adult orga ...
PATO5.08
... • UO – an ontology of units – UO’s top-level division is between primary base units of a particular measure and units that are derived ...
... • UO – an ontology of units – UO’s top-level division is between primary base units of a particular measure and units that are derived ...