Adventitious root formation in rice requires OsGNOM1 and
... limited. We report here the isolation of four rice (Oryza sativa L.) mutants, from different genetic backgrounds, all of which were defective in AR formation. These mutants exhibited reduced numbers of lateral roots (LRs) and partial loss of gravitropism. The mutants also displayed enhanced sensitiv ...
... limited. We report here the isolation of four rice (Oryza sativa L.) mutants, from different genetic backgrounds, all of which were defective in AR formation. These mutants exhibited reduced numbers of lateral roots (LRs) and partial loss of gravitropism. The mutants also displayed enhanced sensitiv ...
Epigenetic Regulation ofbdnfGene Transcription in the
... CpG islands were found within promoters 1 and 2 and the exonic regions of the bdnf gene immediately after the transcriptional start site of exons II, IV, VI, and IX (see Fig. 2 A). This was expected, because region-specific DNA methylation is primarily found in 5⬘-CpG-3⬘-dinucleotides within promote ...
... CpG islands were found within promoters 1 and 2 and the exonic regions of the bdnf gene immediately after the transcriptional start site of exons II, IV, VI, and IX (see Fig. 2 A). This was expected, because region-specific DNA methylation is primarily found in 5⬘-CpG-3⬘-dinucleotides within promote ...
Temperature-Sensitive Alleles of RSW2 Link the KORRIGAN Endo
... An 8.5-kb cosmid containing the KORRIGAN gene complements the cellulose-deficient rsw2-1 mutant of Arabidopsis. Three temperature-sensitive alleles of rsw2 show single amino acid mutations in the putative endo-1,4--glucanase encoded by KOR. The F1 from crosses between kor-1 and rsw2 alleles shows a ...
... An 8.5-kb cosmid containing the KORRIGAN gene complements the cellulose-deficient rsw2-1 mutant of Arabidopsis. Three temperature-sensitive alleles of rsw2 show single amino acid mutations in the putative endo-1,4--glucanase encoded by KOR. The F1 from crosses between kor-1 and rsw2 alleles shows a ...
Community Attitudes to Biotechnology Report on Health and
... This section presents the findings for community attitudes and perceptions of health and medical applications of biotechnology This section details the findings from the quantitative and qualitative phases of research pertaining to health and medical applications of biotechnology. Where it would ass ...
... This section presents the findings for community attitudes and perceptions of health and medical applications of biotechnology This section details the findings from the quantitative and qualitative phases of research pertaining to health and medical applications of biotechnology. Where it would ass ...
Identification of a Class of Chromatin Boundary Elements
... boundary function, we were interested in characterizing a number of these genomic BEAF binding sites to see if they have boundary activity and to derive a consensus sequence and structure for BEAF binding. Genomic BEAF binding sites were isolated as depicted in Fig. 1A. Briefly, appropriate oligonuc ...
... boundary function, we were interested in characterizing a number of these genomic BEAF binding sites to see if they have boundary activity and to derive a consensus sequence and structure for BEAF binding. Genomic BEAF binding sites were isolated as depicted in Fig. 1A. Briefly, appropriate oligonuc ...
A 1. (10 marks) - The University of Western Australia
... useful for examining such tissue? Hint: How big is a red blood cell? (3 marks) (b) What special hazards would you encounter in a laboratory that processed fresh human tissue samples? What precautions would you take? (3 marks) (c) Pararosanilin (C19H18N3Cl) is the active constituent in Feulgen’s reac ...
... useful for examining such tissue? Hint: How big is a red blood cell? (3 marks) (b) What special hazards would you encounter in a laboratory that processed fresh human tissue samples? What precautions would you take? (3 marks) (c) Pararosanilin (C19H18N3Cl) is the active constituent in Feulgen’s reac ...
Overexpression of miR165 Affects Apical
... species and, of them, many putative target genes have been predicted (Dugas and Bartel 2004, Kidner and Martienssen 2005a, Zhang et al. 2006). It has been shown that miRNAs are first transcribed by RNA polymerase II to produce pri-miRNAs, which are then processed by DICER-like proteins and other com ...
... species and, of them, many putative target genes have been predicted (Dugas and Bartel 2004, Kidner and Martienssen 2005a, Zhang et al. 2006). It has been shown that miRNAs are first transcribed by RNA polymerase II to produce pri-miRNAs, which are then processed by DICER-like proteins and other com ...
First mutation in the red blood cell-specific
... of HNSHA due to HK-1 deficiency have been described to date.9 Bianchi et al.10 were the first to demonstrate the molecular defect underlying HK deficiency in a patient with hemolytic anemia. This so-called HK-Melzo variant was due to compound heterozygosity for a 95 bp deletion and a c.1667T>C misse ...
... of HNSHA due to HK-1 deficiency have been described to date.9 Bianchi et al.10 were the first to demonstrate the molecular defect underlying HK deficiency in a patient with hemolytic anemia. This so-called HK-Melzo variant was due to compound heterozygosity for a 95 bp deletion and a c.1667T>C misse ...
Methods of screening for bioactive agents using cells transformed
... for each cell type being analyZed due to inherent differences ...
... for each cell type being analyZed due to inherent differences ...
Induction of apoptosis by Smad3 and down-regulation of
... indicating that the number of cells were still growing. It is noteworthy that when Smad3 was constitutively expressed in the presence of TGF-b (tet7, TGF-b+), luciferase activity decreased significantly from 24 to 48 h, indicating a continued decrease in the number of cells. In contrast, the inducti ...
... indicating that the number of cells were still growing. It is noteworthy that when Smad3 was constitutively expressed in the presence of TGF-b (tet7, TGF-b+), luciferase activity decreased significantly from 24 to 48 h, indicating a continued decrease in the number of cells. In contrast, the inducti ...
A xylem-specific cellulose synthase gene from aspen (Populus
... localization and speci®c function have not been documented. It is also unknown whether the same or different CesA genes are involved in normal and tension wood cellulose formation in angiosperms. Here we characterize a fulllength CesA cDNA (PtCesA) from aspen (Populus tremuloides) and present in sit ...
... localization and speci®c function have not been documented. It is also unknown whether the same or different CesA genes are involved in normal and tension wood cellulose formation in angiosperms. Here we characterize a fulllength CesA cDNA (PtCesA) from aspen (Populus tremuloides) and present in sit ...
Replication origin plasticity, Taylor-made: inhibition vs
... nearly complete stalling, severely inhibited the firing of new origins. These authors concluded that a partial depletion of nucleotide pools inhibits new origins from firing, rather than increasing the number of origins. A second series of studies took advantage of the versatile Xenopus egg extract ...
... nearly complete stalling, severely inhibited the firing of new origins. These authors concluded that a partial depletion of nucleotide pools inhibits new origins from firing, rather than increasing the number of origins. A second series of studies took advantage of the versatile Xenopus egg extract ...
chapter 6
... result from sporadic or inherited mutations, as discussed below. Generally this occurs as a variation of the Knudson-Strong two-hit hypothesis, which theoretically may involve loss of heterozygosity or aberrant gene methylation as well as DNA mutations. Malignant transformation of rhabdomyomas very ...
... result from sporadic or inherited mutations, as discussed below. Generally this occurs as a variation of the Knudson-Strong two-hit hypothesis, which theoretically may involve loss of heterozygosity or aberrant gene methylation as well as DNA mutations. Malignant transformation of rhabdomyomas very ...
Evolution of the bilaterian germ line: lineage origin
... time of their specification during embryogenesis, until the time that gametogenesis begins, usually during larval or adult life (Saffman and Lasko 1999; Gilbert and Singer 2006). They are relatively quiescent transcriptionally during most of embryonic development, as revealed by diagnostic histone m ...
... time of their specification during embryogenesis, until the time that gametogenesis begins, usually during larval or adult life (Saffman and Lasko 1999; Gilbert and Singer 2006). They are relatively quiescent transcriptionally during most of embryonic development, as revealed by diagnostic histone m ...
Bridging the transgenerational gap with epigenetic memory
... type ancestors [27]. The long lifespan of the descendants is dependent on the H3K4me3 demethylase retinoblastoma binding protein related 2 (RBR-2) and requires a functioning germline [27]. This suggests that longevity of wild type descendants from a cross between mutant ancestors and wild type worms ...
... type ancestors [27]. The long lifespan of the descendants is dependent on the H3K4me3 demethylase retinoblastoma binding protein related 2 (RBR-2) and requires a functioning germline [27]. This suggests that longevity of wild type descendants from a cross between mutant ancestors and wild type worms ...
187-192. Control of transcription by Pontin and Reptin
... juxtaposed hexamers, both of which look different than Pontin hexamers; these dodecamers contain a 26 A wide central channel, which would be wide enough to accommodate dsDNA (14). While the dodecamers are made up of equimolar amounts of Pontin and Reptin, it is not clear whether one of the hexamers ...
... juxtaposed hexamers, both of which look different than Pontin hexamers; these dodecamers contain a 26 A wide central channel, which would be wide enough to accommodate dsDNA (14). While the dodecamers are made up of equimolar amounts of Pontin and Reptin, it is not clear whether one of the hexamers ...
Germline stem cells are critical for sexual fate decision of germ cells
... mechanism of sexual fate decision in germ cells may be linked to both the oogenesis process during the development and the germline stem cell status in the mature ovary. The loss of germline stem cells may allow for the neofunctionalization of sox9/amh, which contributes to the masculinization of th ...
... mechanism of sexual fate decision in germ cells may be linked to both the oogenesis process during the development and the germline stem cell status in the mature ovary. The loss of germline stem cells may allow for the neofunctionalization of sox9/amh, which contributes to the masculinization of th ...
Molecular mechanisms of the origin of micronuclei
... its fate in the nucleus of several cell lines with different genetic background was studied (39). The DNA injected at the nuclear environment initially diffused depending on its length. The long DNA (2800 to 15 000 bp) could not move from the injected site, whereas the shorter DNA (400 bp) moved by ...
... its fate in the nucleus of several cell lines with different genetic background was studied (39). The DNA injected at the nuclear environment initially diffused depending on its length. The long DNA (2800 to 15 000 bp) could not move from the injected site, whereas the shorter DNA (400 bp) moved by ...
I • I I
... regulated differentiation of these cell types. Root epidermal development in vascular plants may be divided into three broad groups based on the mode of hair development; Type 1: any cell in the epidermis can form a root hair; Type 2: the smaller product of an asymmetric cell division forms a root h ...
... regulated differentiation of these cell types. Root epidermal development in vascular plants may be divided into three broad groups based on the mode of hair development; Type 1: any cell in the epidermis can form a root hair; Type 2: the smaller product of an asymmetric cell division forms a root h ...
Urbilaterian Reproduction
... could occur in some of the cells’ (Wolpert et al. 2007: 521). More explicitly, ‘The only way for the genome to be fully tested is to have only one line of germ cells’ (Gerhart and Kirschner 1997: 249). Sequestration of a dedicated germ line early in development circumvents this problem, as the organ ...
... could occur in some of the cells’ (Wolpert et al. 2007: 521). More explicitly, ‘The only way for the genome to be fully tested is to have only one line of germ cells’ (Gerhart and Kirschner 1997: 249). Sequestration of a dedicated germ line early in development circumvents this problem, as the organ ...
The ancestral role of Brachyury: expression of NemBra1 in the basal
... Brachyury clusters with other cnidarian Brachyury homologs, for instance the Hydra ortholog HyBra1 (Fig. 1B). However, it is not closely related to a recently identified second Brachyury gene from Hydra, HyBra2 (Technau, unpublished data). We therefore termed the Nematostella Brachyury gene NemBra1. ...
... Brachyury clusters with other cnidarian Brachyury homologs, for instance the Hydra ortholog HyBra1 (Fig. 1B). However, it is not closely related to a recently identified second Brachyury gene from Hydra, HyBra2 (Technau, unpublished data). We therefore termed the Nematostella Brachyury gene NemBra1. ...
PDF
... RUNX2 gene result in autosomal-dominant cleidocranial dysplasia in humans, which is characterized by hypoplasia/aplasia of clavicles, patent fontanelles, supernumerary teeth, short stature and other defects in skeletal patterning and growth (Mundlos et al., 1997; Otto et al., 1997). Here, we show th ...
... RUNX2 gene result in autosomal-dominant cleidocranial dysplasia in humans, which is characterized by hypoplasia/aplasia of clavicles, patent fontanelles, supernumerary teeth, short stature and other defects in skeletal patterning and growth (Mundlos et al., 1997; Otto et al., 1997). Here, we show th ...
Special Feature —Manipulating Genes to Understand
... information) with regions of identical sequence on the chromosome. The greatest advantage of this methodology is the ability to specifically mutate (create a null mutation in) a gene located in the host genome. Because the cells are pluripotent, genetically modified ES cells can be reintroduced into ...
... information) with regions of identical sequence on the chromosome. The greatest advantage of this methodology is the ability to specifically mutate (create a null mutation in) a gene located in the host genome. Because the cells are pluripotent, genetically modified ES cells can be reintroduced into ...
cell culture mycoplasmas
... (e.g. M. arginini, M. hominis) can increase the pH value due to the production of ammonia, which is also a highly toxic agent inhibiting cell growth. Additionally, activity of mycoplasmal arginine deiminase as well as mycoplasmal uptake and depletion of the growth medium were shown to inhibit cell p ...
... (e.g. M. arginini, M. hominis) can increase the pH value due to the production of ammonia, which is also a highly toxic agent inhibiting cell growth. Additionally, activity of mycoplasmal arginine deiminase as well as mycoplasmal uptake and depletion of the growth medium were shown to inhibit cell p ...