Visualization of oligonucleotide probes and point mutations in
... probes for RCA reactions are listed in Tables 1 and 2. The probe-primer ODNs and the AD-P2-ODNs were designed to have two 3⬘ ends. The synthesis of these ODNs was initiated from the 3⬘ end of the primer sequence with reverse amidites and changed to normal nucleotide amidites after the synthesis of a ...
... probes for RCA reactions are listed in Tables 1 and 2. The probe-primer ODNs and the AD-P2-ODNs were designed to have two 3⬘ ends. The synthesis of these ODNs was initiated from the 3⬘ end of the primer sequence with reverse amidites and changed to normal nucleotide amidites after the synthesis of a ...
5.MESODERM FORMATION / SEGMENTATION
... vertebral rudiments are actually formed intersegmentally by the fusion of the caudal half of one sclerotome with the cranial aspect of the next (Fig. 5-12). The cells on the dorsal aspect of the somite which remain compact after the de-epithelialization of the sclerotome are called the dermamyotome ...
... vertebral rudiments are actually formed intersegmentally by the fusion of the caudal half of one sclerotome with the cranial aspect of the next (Fig. 5-12). The cells on the dorsal aspect of the somite which remain compact after the de-epithelialization of the sclerotome are called the dermamyotome ...
Disrupting antibiotic resistance propagation by inhibiting
... relaxases are capable of accommodating two phosphotyrosine intermediates simultaneously within their active sites. The need to handle dual phosphotyrosine intermediates would explain the key features of the TraI relaxase outlined above: the presence of two catalytically competent tyrosines and the r ...
... relaxases are capable of accommodating two phosphotyrosine intermediates simultaneously within their active sites. The need to handle dual phosphotyrosine intermediates would explain the key features of the TraI relaxase outlined above: the presence of two catalytically competent tyrosines and the r ...
Acute stress and hippocampal histone H3 lysine 9 trimethylation, a
... loci (Fig. 3A) [false-discovery rate (FDR) <0.0001, P < 0.05]. When categorized by repetitive-element class, differences emerge in the enrichment of stress-up versus stress-down peaks: the autonomous long interspersed element (LINE) retrotransposons and endogenous retrovirus/long terminal repeat (LT ...
... loci (Fig. 3A) [false-discovery rate (FDR) <0.0001, P < 0.05]. When categorized by repetitive-element class, differences emerge in the enrichment of stress-up versus stress-down peaks: the autonomous long interspersed element (LINE) retrotransposons and endogenous retrovirus/long terminal repeat (LT ...
Role of Template Activating Factor-I as a
... Fig. 2. In vitro linker histone chaperone activity of TAF-I. (A) Purified recombinant proteins. Recombinant GST, GST–TAF-Ib, His–H1.1 proteins were separated by 10% SDS-PAGE and visualized with Coomassie Brilliant Blue staining. Lane M contains molecular size markers. (B) Formation of histone H1.1NC ...
... Fig. 2. In vitro linker histone chaperone activity of TAF-I. (A) Purified recombinant proteins. Recombinant GST, GST–TAF-Ib, His–H1.1 proteins were separated by 10% SDS-PAGE and visualized with Coomassie Brilliant Blue staining. Lane M contains molecular size markers. (B) Formation of histone H1.1NC ...
The β-Globin LCR is Not Necessary for an Open Chromatin
... LCR in ES cells deleted prior to and after chromosome transfer to K562 cells ...
... LCR in ES cells deleted prior to and after chromosome transfer to K562 cells ...
- SciTech Connect
... of oskar and bicoid mRNAs, which is established during oogenesis.9,10 Mislocalization of either oskar or bicoid mRNA in the oocyte leads to defects in segment specification, such as defective heads in the embryo.9,10 These and other studies demonstrate that maternally supplied RNAs are required for ...
... of oskar and bicoid mRNAs, which is established during oogenesis.9,10 Mislocalization of either oskar or bicoid mRNA in the oocyte leads to defects in segment specification, such as defective heads in the embryo.9,10 These and other studies demonstrate that maternally supplied RNAs are required for ...
Epigenetic Control of Apomixis
... regions of chromosomes and the repetitive elements are heavily methylated. The level of 5-mC is determined by DNA methylation and demethylation processes. Methylation can be removed from the DNA by active and/or passive mechanisms. Promiscuous methylation requires to be pruned by demethylases to cre ...
... regions of chromosomes and the repetitive elements are heavily methylated. The level of 5-mC is determined by DNA methylation and demethylation processes. Methylation can be removed from the DNA by active and/or passive mechanisms. Promiscuous methylation requires to be pruned by demethylases to cre ...
Sex-linked dosage-sensitive modifiers as imprinting
... result of dosage-sensitive modifier genes located on the sex chromosomes. Parallels between variegating position-effects in Drosophila, the phenotype elicited by transgenes in the mouse and data from several pediatric tumors indicate that the net result of the activity of such modifier genes is ofte ...
... result of dosage-sensitive modifier genes located on the sex chromosomes. Parallels between variegating position-effects in Drosophila, the phenotype elicited by transgenes in the mouse and data from several pediatric tumors indicate that the net result of the activity of such modifier genes is ofte ...
Aberrant replication timing induces defective chromosome
... sister chromatids, suggested that euchromatin may be more prone to defective condensation than heterochromatin. This could be explained if condensation defects were a consequence of aberrant DNA replication. For example, alterations in the replication timing of particular loci might lead to changes ...
... sister chromatids, suggested that euchromatin may be more prone to defective condensation than heterochromatin. This could be explained if condensation defects were a consequence of aberrant DNA replication. For example, alterations in the replication timing of particular loci might lead to changes ...
Pulling forces acting on Hox gene clusters cause expression
... expression intensity systematically increases following the order Hoxd10,…,Hoxd13. The above features of Hox gene collinearity have been meticulously analyzed for more than 25 years in many organisms ranging from Drosophila to humans. Many attempts have been made to justify these surprising regulari ...
... expression intensity systematically increases following the order Hoxd10,…,Hoxd13. The above features of Hox gene collinearity have been meticulously analyzed for more than 25 years in many organisms ranging from Drosophila to humans. Many attempts have been made to justify these surprising regulari ...
Efficient delivery of small interfering RNA to plant cells
... lines. The gfp transgenic cells treated with LISW but no siRNA, or treated with siRNA but no LISW demonstrated no decrease in mRNA levels (Fig. 3B), which has the same levels of mRNA from transgenic cells not treated with siRNA and LISW. The same silencing signature was observed in all three transge ...
... lines. The gfp transgenic cells treated with LISW but no siRNA, or treated with siRNA but no LISW demonstrated no decrease in mRNA levels (Fig. 3B), which has the same levels of mRNA from transgenic cells not treated with siRNA and LISW. The same silencing signature was observed in all three transge ...
Chromatin Domain Boundaries: Defining the Functional Domains in
... position effect assay described above, an enhancerblocking assay for DNA segments that can function as boundaries in vivo have been developed. In this assay instead of blocking of enhancer and silencer in the genomic context, test DNA is assayed for its ability to block a given enhancer from acting ...
... position effect assay described above, an enhancerblocking assay for DNA segments that can function as boundaries in vivo have been developed. In this assay instead of blocking of enhancer and silencer in the genomic context, test DNA is assayed for its ability to block a given enhancer from acting ...
PDF + SI - Development - The Company of Biologists
... Increases in WOX14 expression were not dependent on functional WOX4, as a clear WOX14 response to TDIF was observed in wox4 mutant lines (Fig. 3), demonstrating that WOX14 is not a downstream target of WOX4. Inflorescence stems represent a developmental series with most recently derived vascular tis ...
... Increases in WOX14 expression were not dependent on functional WOX4, as a clear WOX14 response to TDIF was observed in wox4 mutant lines (Fig. 3), demonstrating that WOX14 is not a downstream target of WOX4. Inflorescence stems represent a developmental series with most recently derived vascular tis ...
The budding index of Saccharomyces cerevisiae
... While VPS24 scored positive on both rounds of observation, VPS30 scored positive only once (Table 2). Both deletion strains do not proliferate slower than the wildtype strain (data not shown, and [2]). Based on DNA content analysis, deletion of either VPS24 or VPS30 increased the percentage of cells ...
... While VPS24 scored positive on both rounds of observation, VPS30 scored positive only once (Table 2). Both deletion strains do not proliferate slower than the wildtype strain (data not shown, and [2]). Based on DNA content analysis, deletion of either VPS24 or VPS30 increased the percentage of cells ...
Imprinting and Seed Development
... Maternally inherited 10L cannot compensate for the lack of paternally inherited 10L. The author concluded that these results are evidence of an imprinting effect in which the genes function when inherited paternally. Scott et al. (1998) also found that the source of the extra parental genome(s) affe ...
... Maternally inherited 10L cannot compensate for the lack of paternally inherited 10L. The author concluded that these results are evidence of an imprinting effect in which the genes function when inherited paternally. Scott et al. (1998) also found that the source of the extra parental genome(s) affe ...
Microtubule Polarity and Dynamics in the Control of Organelle
... the case. The average mature flagellum length in any cell cycle stage remained essentially at a constant length of 22.3 ~m + 0.1 (mean + S.E.). Thus, for every individual cytoskeleton measured, the position of each organelle was normalized by expressing the value relative to the length of the mature ...
... the case. The average mature flagellum length in any cell cycle stage remained essentially at a constant length of 22.3 ~m + 0.1 (mean + S.E.). Thus, for every individual cytoskeleton measured, the position of each organelle was normalized by expressing the value relative to the length of the mature ...
Unit 3
... daughter cells is called cell division. Each daughter cell contains an exact copy of the DNA found in the parent cell. Cell division in As you learned earlier, prokaryotic cells like bacteria do not have a prokaryotic cells nucleus. Their DNA is found bunched up in the cytoplasm. Because of this, th ...
... daughter cells is called cell division. Each daughter cell contains an exact copy of the DNA found in the parent cell. Cell division in As you learned earlier, prokaryotic cells like bacteria do not have a prokaryotic cells nucleus. Their DNA is found bunched up in the cytoplasm. Because of this, th ...
Defining the Epigenetic Mechanism of Asymmetric Cell Division of
... generating cellular diversity required for development in multicellular organisms. As one of the potential mechanisms, chromosomally borne epigenetic difference between sister cells that changes mating/cell type has been demonstrated only in the Schizosaccharomyces pombe fission yeast. For technical ...
... generating cellular diversity required for development in multicellular organisms. As one of the potential mechanisms, chromosomally borne epigenetic difference between sister cells that changes mating/cell type has been demonstrated only in the Schizosaccharomyces pombe fission yeast. For technical ...
the regulation of the differential expression of the human globin
... an attempt to understand the mechanism of the differential expression of their constituent loci during development. Comparisons of the primary sequences of the individual genes and their flanking regions have shown that, in general, they rapidly lose homology upstream and downstream of the transcrib ...
... an attempt to understand the mechanism of the differential expression of their constituent loci during development. Comparisons of the primary sequences of the individual genes and their flanking regions have shown that, in general, they rapidly lose homology upstream and downstream of the transcrib ...
Validated preCRMs
... Orkin 1993), and this protein is required for late erythroid maturation (Pevny et al. 1991). The mouse G1E cell line, derived from Gata1 knock-out embryonic stem cells, is blocked at the level of an immature committed erythroblast (Weiss et al. 1997) and undergoes terminal erythroid maturation when ...
... Orkin 1993), and this protein is required for late erythroid maturation (Pevny et al. 1991). The mouse G1E cell line, derived from Gata1 knock-out embryonic stem cells, is blocked at the level of an immature committed erythroblast (Weiss et al. 1997) and undergoes terminal erythroid maturation when ...
Drosophila windpipe codes for a leucine
... 472 form a transmembrane domain, followed by an acidic-rich ‘stop-transfer’ sequence (KRKC). The predicted extracellular domain contains four putative LRR motifs spanning amino acid residues 70–92, 93–118, 135–157 and 167–216. LRRs are found in numerous proteins and function in mediating protein–pro ...
... 472 form a transmembrane domain, followed by an acidic-rich ‘stop-transfer’ sequence (KRKC). The predicted extracellular domain contains four putative LRR motifs spanning amino acid residues 70–92, 93–118, 135–157 and 167–216. LRRs are found in numerous proteins and function in mediating protein–pro ...
Identifying Genes Required for Cell Division in the Early C. elegans
... cell division and subsequent development of the zygote. The spindle facilitates chromosome segregation to properly distribute genetic information to newly formed daughter cells. Four C. elegans temperature-sensitive mutants are examined in this paper that have defects in spindle formation or functio ...
... cell division and subsequent development of the zygote. The spindle facilitates chromosome segregation to properly distribute genetic information to newly formed daughter cells. Four C. elegans temperature-sensitive mutants are examined in this paper that have defects in spindle formation or functio ...
To Я or Not To Я: Estrogen Receptors and Ovarian Function
... (26). Equally exciting is the growing body of evidence suggesting early actions of estrogens in follicle formation, maturation or atresia, and, indeed, in regulating the sexual differentiation of ovarian granulosa cells. The evidence thus far suggests that these actions are also likely to be mediate ...
... (26). Equally exciting is the growing body of evidence suggesting early actions of estrogens in follicle formation, maturation or atresia, and, indeed, in regulating the sexual differentiation of ovarian granulosa cells. The evidence thus far suggests that these actions are also likely to be mediate ...
B-catenin
... • To pursue the role of Wnt activation in the islet, it would be desirable to have an in vitro model. Thus, we examined Wnt activation in isolated islets. • Surprisingly, when cultured human islets were examined by Western blotting, βcatenin, which is low or absent in the islet compared with surro ...
... • To pursue the role of Wnt activation in the islet, it would be desirable to have an in vitro model. Thus, we examined Wnt activation in isolated islets. • Surprisingly, when cultured human islets were examined by Western blotting, βcatenin, which is low or absent in the islet compared with surro ...