GeNeViSTA Coffin Siris Syndrome: A Disorder of SWI/SNF Pathway
... cell nucleus in the form of nucleosomes, which are formed by ∼147 bp of DNA wrapped around histone proteins. Nucleosomes are assembled into condensed chromatin which inhibits access to DNA for cellular proteins that drive chromatin-based processes, including transcription and DNA repair. An importan ...
... cell nucleus in the form of nucleosomes, which are formed by ∼147 bp of DNA wrapped around histone proteins. Nucleosomes are assembled into condensed chromatin which inhibits access to DNA for cellular proteins that drive chromatin-based processes, including transcription and DNA repair. An importan ...
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... Herrmann, 1994). Correspondingly, the phenotype of Brachyury mutations in mouse can be rescued by a transgene of the wild-type T allele (Stott et al., 1993). (2) Studies by Kispert et al. (1995) and Conlon et al. (1996) have shown that Brachyury functions as a transcriptional activator. Not only do ...
... Herrmann, 1994). Correspondingly, the phenotype of Brachyury mutations in mouse can be rescued by a transgene of the wild-type T allele (Stott et al., 1993). (2) Studies by Kispert et al. (1995) and Conlon et al. (1996) have shown that Brachyury functions as a transcriptional activator. Not only do ...
An Arabidopsis Mutant with a Reduced Leve1 of cabl40 RNA 1s a
... We analyzed a mutant of Arabidopsis with a severely reduced level of cabl40 RNA. This mutant, named Ict for Iow level of cabl40 transcript, was obtained during a selection for phytochrome signal transduction mutants. The selection was based on reduced expression of the tumor morphology shoots gene ( ...
... We analyzed a mutant of Arabidopsis with a severely reduced level of cabl40 RNA. This mutant, named Ict for Iow level of cabl40 transcript, was obtained during a selection for phytochrome signal transduction mutants. The selection was based on reduced expression of the tumor morphology shoots gene ( ...
Intercellular signals downstream of endothelin
... thelin-1, endothelin-2, endothelin-3 and vasoactive intestinal contractor (Sokolovsky, 1994). At present, two G-coupled, serpentine receptors, ET-A and ET-B, have been identified for endothelin peptides. ET-B interacts with all endothelin peptides with approximately equal affinities, whereas ET-A is ...
... thelin-1, endothelin-2, endothelin-3 and vasoactive intestinal contractor (Sokolovsky, 1994). At present, two G-coupled, serpentine receptors, ET-A and ET-B, have been identified for endothelin peptides. ET-B interacts with all endothelin peptides with approximately equal affinities, whereas ET-A is ...
Tissue Engineering for In Vitro Analysis of Matrix Metalloproteinases
... Effect of Decorin on Keloid Fibroblasts in the Matrigel ModelLetters indicate statistical significance from HS27 at corresponding time point (P < .01b); plus signs, statistical significance from day 7 of same cell type (P < .001a). A, DNA quantification normalized to respective dry weights indicated ...
... Effect of Decorin on Keloid Fibroblasts in the Matrigel ModelLetters indicate statistical significance from HS27 at corresponding time point (P < .01b); plus signs, statistical significance from day 7 of same cell type (P < .001a). A, DNA quantification normalized to respective dry weights indicated ...
Apoptotic and Epigenetic Induction of Embryo Failure Following
... Because high rates of apoptosis are associated with failure of preimplantation embryos, we compared the activation of the P53-mediated apoptosis pathway in individual IVF and SCNT preimplantation embryos at multiple developmental stages. This pathway is activated in response to cell stress and genom ...
... Because high rates of apoptosis are associated with failure of preimplantation embryos, we compared the activation of the P53-mediated apoptosis pathway in individual IVF and SCNT preimplantation embryos at multiple developmental stages. This pathway is activated in response to cell stress and genom ...
Commentaries on Viewpoint: Epigenetic regulation of the ACE gene
... whether epigenetic regulation of the ACE gene is specifically involved in modifying human endurance. However, we believe that establishing the epigenetic status of the ACE gene in participants involved in endurance is not trivial. As stated by Raleigh, tissue-specific methylation of the ACE gene wou ...
... whether epigenetic regulation of the ACE gene is specifically involved in modifying human endurance. However, we believe that establishing the epigenetic status of the ACE gene in participants involved in endurance is not trivial. As stated by Raleigh, tissue-specific methylation of the ACE gene wou ...
Anther Development
... with the filament (Figures 3 and 4). Other tissues, such as the circular cell cluster and stomium, form at the boundaries between each microsporangial pair later in development (Figures 3 and 4). Thus, specific regions, or territories, are established early in anther development within which unique ...
... with the filament (Figures 3 and 4). Other tissues, such as the circular cell cluster and stomium, form at the boundaries between each microsporangial pair later in development (Figures 3 and 4). Thus, specific regions, or territories, are established early in anther development within which unique ...
Mechanisms of tumour development
... et al., Cancer Epidemiol Biomarkers Prev, 9: 127-137, 2000). Additionally, genetic lesions such as progressive genomic instability as measured by loss of heterozygosity or amplification at specific microsatellite loci, have been considered (Califano J et al. Cancer Res, 56: 2488-2492, 1996). Other p ...
... et al., Cancer Epidemiol Biomarkers Prev, 9: 127-137, 2000). Additionally, genetic lesions such as progressive genomic instability as measured by loss of heterozygosity or amplification at specific microsatellite loci, have been considered (Califano J et al. Cancer Res, 56: 2488-2492, 1996). Other p ...
Lineage-specific stem cells, signals and asymmetries
... (Iwafuchi-Doi and Zaret, 2014). For instance, ectopic expression of MyoD in fibroblasts induces muscle cell differentiation (Davis et al., 1987). The results of genome-wide MyoD binding studies indicate that MyoD binding requires specific E-boxes and is dependent on both interacting factors (coopera ...
... (Iwafuchi-Doi and Zaret, 2014). For instance, ectopic expression of MyoD in fibroblasts induces muscle cell differentiation (Davis et al., 1987). The results of genome-wide MyoD binding studies indicate that MyoD binding requires specific E-boxes and is dependent on both interacting factors (coopera ...
Overexpression of the catalytic subunit of DNA polymerase results in
... genomic DNAs of stably transformed Schneider cell lines. Total DNA (5 µg) from control Schneider cells (lane 1), and stable Schneider cells containing the pMt/Hy vector (lane 2) or the pMt/ 125/Hy construct was digested with EcoRI, electrophoresed in a 1% agarose gel, transferred to nitrocellulose a ...
... genomic DNAs of stably transformed Schneider cell lines. Total DNA (5 µg) from control Schneider cells (lane 1), and stable Schneider cells containing the pMt/Hy vector (lane 2) or the pMt/ 125/Hy construct was digested with EcoRI, electrophoresed in a 1% agarose gel, transferred to nitrocellulose a ...
Functional genomics identifies a Myb domain– containing protein
... at http://www.jcb.org/cgi/content/full/jcb.200701065/DC1). Both tools revealed that KNL-2 localizes to the centromere/kinetochore region throughout mitosis (Fig. 1, D and E). The specificity of antibody localization was established using RNAi-mediated protein depletion (Fig. 2 A). Because our screen ...
... at http://www.jcb.org/cgi/content/full/jcb.200701065/DC1). Both tools revealed that KNL-2 localizes to the centromere/kinetochore region throughout mitosis (Fig. 1, D and E). The specificity of antibody localization was established using RNAi-mediated protein depletion (Fig. 2 A). Because our screen ...
NIH Public Access - University of Chicago
... The transcription factors EBF and Pax5 play major roles in B cell fate specification and commitment by activating B lineage genes and concomitantly repressing genes of alternate lineages8,19,20. EBF initiates restriction of alternate lineage options and B cell fate commitment independently of Pax5 ( ...
... The transcription factors EBF and Pax5 play major roles in B cell fate specification and commitment by activating B lineage genes and concomitantly repressing genes of alternate lineages8,19,20. EBF initiates restriction of alternate lineage options and B cell fate commitment independently of Pax5 ( ...
Cys mutation of fibroblast growth factor receptor 3 in mouse
... important roles in chondrocyte proliferation and differentiation and that FGFR3 signaling appears to downregulate the expression of IHH (2–4,25–27), it is possible that these two pathways interact functionally during endochondral bone growth. To shed light on such interactions and their role in skel ...
... important roles in chondrocyte proliferation and differentiation and that FGFR3 signaling appears to downregulate the expression of IHH (2–4,25–27), it is possible that these two pathways interact functionally during endochondral bone growth. To shed light on such interactions and their role in skel ...
The Alexander Silberman Institute of Life Sciences Departements
... malarial parasite membranes. Biochemistry and physiology of energy and oxidative metabolism of malarial parasites. Mode of antimalarial action of quinoline containing drugs and the molecular basis of drug resistance. Development of new antimalarial drugs. Mathematical modeling of the chemotherapy of ...
... malarial parasite membranes. Biochemistry and physiology of energy and oxidative metabolism of malarial parasites. Mode of antimalarial action of quinoline containing drugs and the molecular basis of drug resistance. Development of new antimalarial drugs. Mathematical modeling of the chemotherapy of ...
Commentary: Lamarckian inheritance and
... the genome organizes its components. Epigenetics is no ...
... the genome organizes its components. Epigenetics is no ...
Embryonic fat-cell lineage in Drosophila melanogaster
... heart muscle (Bodmer et al., 1990; Azpiazu and Frasch, 1993; Bodmer, 1993) and disrupts the development of the midgut visceral mesoderm (Azpiazu and Frasch, 1993). These data suggest bap and tin may be involved in specifying cell fate within the mesoderm. Part of the mesoderm gives rise to the Droso ...
... heart muscle (Bodmer et al., 1990; Azpiazu and Frasch, 1993; Bodmer, 1993) and disrupts the development of the midgut visceral mesoderm (Azpiazu and Frasch, 1993). These data suggest bap and tin may be involved in specifying cell fate within the mesoderm. Part of the mesoderm gives rise to the Droso ...
Characterization of the metacaspase gene family in Arabidopsis
... MCs in the two algae is believed to be due to gene loss from environmental adaptation (Cambra et al. 2010), and currently there is no known connection between the number of MC type I vs. MC type II within different species. Another difference between the two MC types is the presence of a LSD1-likefi ...
... MCs in the two algae is believed to be due to gene loss from environmental adaptation (Cambra et al. 2010), and currently there is no known connection between the number of MC type I vs. MC type II within different species. Another difference between the two MC types is the presence of a LSD1-likefi ...
Is HP1 an RNA detector that functions both in repression and
... protein; it is capable of interacting with a host of proteins with a range of nuclear activities (Kellum, 2003; and references therein). Do RNA sense strands recruit transcriptional activators to sites of active transcription through HP1, while RNA double strands specify targeting of a separate clas ...
... protein; it is capable of interacting with a host of proteins with a range of nuclear activities (Kellum, 2003; and references therein). Do RNA sense strands recruit transcriptional activators to sites of active transcription through HP1, while RNA double strands specify targeting of a separate clas ...
Cloning, expression, sequence analysis and
... vegetative cell walls were used for detection of lytic activity (Jayaswal et al., 1990). Following electrophoresis, gels were rinsed in distilled water and then renatured in 1 % (v/v) Triton X-100 (Aldrich), 20 mMMgCI2, 25 mM-Tris/HCl, pH 7-5, (3 x 30 min, 20 "C). Gels were then incubated for 16 h a ...
... vegetative cell walls were used for detection of lytic activity (Jayaswal et al., 1990). Following electrophoresis, gels were rinsed in distilled water and then renatured in 1 % (v/v) Triton X-100 (Aldrich), 20 mMMgCI2, 25 mM-Tris/HCl, pH 7-5, (3 x 30 min, 20 "C). Gels were then incubated for 16 h a ...
A Symbolic and Graphical Gene Regulation Model of the lac Operon
... included into the DNA strand. The lacZ gene, with its associated control complex is incorporated into the DNA structure. In addition, the lacI gene along with its control complex is also part of the visualization. All other interactive elements including RNA polymerase, repressor molecules, and b-ga ...
... included into the DNA strand. The lacZ gene, with its associated control complex is incorporated into the DNA structure. In addition, the lacI gene along with its control complex is also part of the visualization. All other interactive elements including RNA polymerase, repressor molecules, and b-ga ...
LIN-5 Is a Novel Component of the Spindle Apparatus
... (McKim and Hawley, 1995). However, in most cell divisions, the MTOCs duplicate, separate to opposite poles, and form two sites for microtubule nucleation. In this way, MTOCs contribute to the bipolar nature of the spindle, as well as to rapid and directional assembly of microtubules. The mitotic fu ...
... (McKim and Hawley, 1995). However, in most cell divisions, the MTOCs duplicate, separate to opposite poles, and form two sites for microtubule nucleation. In this way, MTOCs contribute to the bipolar nature of the spindle, as well as to rapid and directional assembly of microtubules. The mitotic fu ...
RealTime ready Cell Lysis Kit
... represents a beneficial building block in the pathway of gene expression analysis by providing significant time savings combined with increased convenience for low- to medium-throughput, real-time PCR applications. Together with the Transcriptor Universal cDNA Master, with its ...
... represents a beneficial building block in the pathway of gene expression analysis by providing significant time savings combined with increased convenience for low- to medium-throughput, real-time PCR applications. Together with the Transcriptor Universal cDNA Master, with its ...
Different physical delivery systems: An important approach for
... been successfully used to transfer genes into various cell lines [8]. In vivo applications have extensively focused on the skin, muscle, liver or other organs that can be exposed after surgical procedure. Unfortunately, genes delivered by this method are expressed transiently, and there is considera ...
... been successfully used to transfer genes into various cell lines [8]. In vivo applications have extensively focused on the skin, muscle, liver or other organs that can be exposed after surgical procedure. Unfortunately, genes delivered by this method are expressed transiently, and there is considera ...
Visualization of oligonucleotide probes and point mutations in
... probes for RCA reactions are listed in Tables 1 and 2. The probe-primer ODNs and the AD-P2-ODNs were designed to have two 3⬘ ends. The synthesis of these ODNs was initiated from the 3⬘ end of the primer sequence with reverse amidites and changed to normal nucleotide amidites after the synthesis of a ...
... probes for RCA reactions are listed in Tables 1 and 2. The probe-primer ODNs and the AD-P2-ODNs were designed to have two 3⬘ ends. The synthesis of these ODNs was initiated from the 3⬘ end of the primer sequence with reverse amidites and changed to normal nucleotide amidites after the synthesis of a ...