
Genes with ectopic expression phenotypes are common, not rare
... Traditionally, gene function in model organisms has been studied through analysis of loss-offunction mutant phenotypes. Gene redundancy, however, poses the limitation that not all genes have a loss-of-function phenotype. To the extent that overexpression of genes causes detrimental phenotypes, ectop ...
... Traditionally, gene function in model organisms has been studied through analysis of loss-offunction mutant phenotypes. Gene redundancy, however, poses the limitation that not all genes have a loss-of-function phenotype. To the extent that overexpression of genes causes detrimental phenotypes, ectop ...
Figures and figure supplements
... cells. Switches are labeled with the numbers 1 and 2. In pedigree A, onset of GFP expression (depicted as yellow) was detected in a large-budded cell (1). At the same time, GFP also appeared in the daughter cell that arose from the previous cell division (2). Due to the assymetry of switch 1 (only t ...
... cells. Switches are labeled with the numbers 1 and 2. In pedigree A, onset of GFP expression (depicted as yellow) was detected in a large-budded cell (1). At the same time, GFP also appeared in the daughter cell that arose from the previous cell division (2). Due to the assymetry of switch 1 (only t ...
Sickle Cell Anemia Answer Key - Illuminations
... Sickle cell disease: a genetic disease Sickle trait: a genotype with one sickle and one normal gene. People with sickle trait are immune to malaria. Normal: a genotype that does not have any potential for passing on a sickle gene Allele: a gene from one parent. One gene from each parent combined mak ...
... Sickle cell disease: a genetic disease Sickle trait: a genotype with one sickle and one normal gene. People with sickle trait are immune to malaria. Normal: a genotype that does not have any potential for passing on a sickle gene Allele: a gene from one parent. One gene from each parent combined mak ...
The Arabidopsis BET Bromodomain Factor GTE4
... Bromodomain and Extra Terminal domain (BET) proteins are characterized by the presence of two types of domains, the bromodomain and the extra terminal domain. They bind to acetylated lysines present on histone tails and control gene transcription. They are also well known to play an important role i ...
... Bromodomain and Extra Terminal domain (BET) proteins are characterized by the presence of two types of domains, the bromodomain and the extra terminal domain. They bind to acetylated lysines present on histone tails and control gene transcription. They are also well known to play an important role i ...
Nucleoid occlusion and bacterial cell division
... Furthermore, when replication fork arrest was induced by creating a road block on one arm of the chromosome, cell division over the nucleoid was inhibited by a Noc-independent mechanism20. Altering the organization of the nucleoid partially relieved this inhibition, suggesting that the inhibition wa ...
... Furthermore, when replication fork arrest was induced by creating a road block on one arm of the chromosome, cell division over the nucleoid was inhibited by a Noc-independent mechanism20. Altering the organization of the nucleoid partially relieved this inhibition, suggesting that the inhibition wa ...
Cloning and functional analysis of the chitinase gene promoter in
... Peanut plants are often attacked by pathogens that cause diseases such as mesh spot, leaf spot, black rot, rust, and bacterial wilt. These diseases reduce seed quality and yield; unfortunately, peanut germplasm resources with high resistance to disease are rare (Wang and Zhang, 2013). One solution t ...
... Peanut plants are often attacked by pathogens that cause diseases such as mesh spot, leaf spot, black rot, rust, and bacterial wilt. These diseases reduce seed quality and yield; unfortunately, peanut germplasm resources with high resistance to disease are rare (Wang and Zhang, 2013). One solution t ...
Myb genes enhance tobacco trichome production
... determined by the plant organ or organ surface (ie, adaxial vs. abaxial, leaf vs. stem) from which the trichome extends, and the developmental phase of the plant at the time of organ initiation. The trichome is a useful cell type for studying regulation of cell differentiation in plants. It is morph ...
... determined by the plant organ or organ surface (ie, adaxial vs. abaxial, leaf vs. stem) from which the trichome extends, and the developmental phase of the plant at the time of organ initiation. The trichome is a useful cell type for studying regulation of cell differentiation in plants. It is morph ...
View as PDF
... The presence of Sir2p in the nucleolus suggests a direct effect on rDNA chromatin, perhaps through modulation of nucleosomal organization within the RNA PolI or PolIII promoter regions (4, 8, 40). In aging yeast cells, or in strains carrying mutant forms of Sir4p, Sir3p also relocalizes from telomer ...
... The presence of Sir2p in the nucleolus suggests a direct effect on rDNA chromatin, perhaps through modulation of nucleosomal organization within the RNA PolI or PolIII promoter regions (4, 8, 40). In aging yeast cells, or in strains carrying mutant forms of Sir4p, Sir3p also relocalizes from telomer ...
PDF + SI - Development - The Company of Biologists
... This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. ...
... This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. ...
Roux`s Arch Dev Biol 193, 283
... single lines were established for each of the three mutagenized genotypes. A total of 257 lines were derived, of which 112 did not produce homozygous flies in the F2 generation. The frequency of 44% lethals corresponds to approximately 0.6 lethal hits per chromosome. This value is much lower than ex ...
... single lines were established for each of the three mutagenized genotypes. A total of 257 lines were derived, of which 112 did not produce homozygous flies in the F2 generation. The frequency of 44% lethals corresponds to approximately 0.6 lethal hits per chromosome. This value is much lower than ex ...
Hormone Autotrophic Growth and Differentiation
... that normally regulate cell division and differentiation and prevent tumor formation. In light of the close links between tumor formation and auxin and cytokinin metabolism and signaling, we hypothesized that this approach would lead to the identification of potential new hormone mutants. Negative c ...
... that normally regulate cell division and differentiation and prevent tumor formation. In light of the close links between tumor formation and auxin and cytokinin metabolism and signaling, we hypothesized that this approach would lead to the identification of potential new hormone mutants. Negative c ...
COP9: A New Genetic Locus lnvolved in Light
... and a domain homologous to the p subunit of trimeric G-proteins (Deng et al., 1992). This nove1 structure suggests that the COR gene product not only has the potential to directly bind DNA through its Zn binding domain, it may also have the ability to interact with other protein components through i ...
... and a domain homologous to the p subunit of trimeric G-proteins (Deng et al., 1992). This nove1 structure suggests that the COR gene product not only has the potential to directly bind DNA through its Zn binding domain, it may also have the ability to interact with other protein components through i ...
Cell Division Studies of Escherichia Coli: Expression and Protein
... Progress has been made in understanding cell division at the genetic level. Conditional lethal mutants that cannot form septa have been isolated and referred to as filamentation temperature-sensitive (fts) mutants. septum are ...
... Progress has been made in understanding cell division at the genetic level. Conditional lethal mutants that cannot form septa have been isolated and referred to as filamentation temperature-sensitive (fts) mutants. septum are ...
the lysis/lysogeny switch in phage \lambda
... behaviour of this induction by UV (or hunger) is a direct consequence of the cooperativity in the repressor mechanism. A hypothetical analog with no cooperativity does not lead to the same switch-like behaviour. ...
... behaviour of this induction by UV (or hunger) is a direct consequence of the cooperativity in the repressor mechanism. A hypothetical analog with no cooperativity does not lead to the same switch-like behaviour. ...
Radiation Hybrid Mapping: A Somatic Cell Genetic Method for
... chromosome corresponds to 1 megabase pairs (Mb) of DNA. In situ hybridization can localize markers to within 2 percent of total chromosome length, but in molecular terms, this again represents several million base pairs. Pulsed-field gel electrophoresis (PFGE), which can separate DNA fragments of se ...
... chromosome corresponds to 1 megabase pairs (Mb) of DNA. In situ hybridization can localize markers to within 2 percent of total chromosome length, but in molecular terms, this again represents several million base pairs. Pulsed-field gel electrophoresis (PFGE), which can separate DNA fragments of se ...
PDF
... basal keratinocytes, we sought to isolate its regulatory regions as a means of driving exogenous genes in this cell layer. This gene sits within a type I keratin gene cluster on chromosome 19 (Figure 2A), with the start of transcription only 3.9 kb downstream from the end of the preceding gene (cki ...
... basal keratinocytes, we sought to isolate its regulatory regions as a means of driving exogenous genes in this cell layer. This gene sits within a type I keratin gene cluster on chromosome 19 (Figure 2A), with the start of transcription only 3.9 kb downstream from the end of the preceding gene (cki ...
Chapter 10.2 ppt
... Cytokinesis usually occurs a. at the same time as telophase. b. after telophase. c. during interphase. ...
... Cytokinesis usually occurs a. at the same time as telophase. b. after telophase. c. during interphase. ...
Nonconventional Initiation Complex Assembly by STAT and NF
... A. Macrophages with IFN-I + hkL -> increase of histone acetylation at both the proximal and distal promoter locations B. Treatment with IFN-I -> increase of H4 acetylation at the distal IFN response region C. hkL treatment -> increase in H4 acetylation at the proximal NF-κB element ISGF3 and NF-κB ...
... A. Macrophages with IFN-I + hkL -> increase of histone acetylation at both the proximal and distal promoter locations B. Treatment with IFN-I -> increase of H4 acetylation at the distal IFN response region C. hkL treatment -> increase in H4 acetylation at the proximal NF-κB element ISGF3 and NF-κB ...
functional analysis of chromatin assembly genes in tetrahymena
... The basic structural unit of chromatin is the nucleosome composed of ~147 base pairs of DNA wrapped around an octamer of histone proteins. Post-translational modifications such as histone acetylation or the substitution of histone variants in place of core histones have been implicated in various ch ...
... The basic structural unit of chromatin is the nucleosome composed of ~147 base pairs of DNA wrapped around an octamer of histone proteins. Post-translational modifications such as histone acetylation or the substitution of histone variants in place of core histones have been implicated in various ch ...
A protocol for mosaic analysis with a repressible cell
... tic recombination, homozygous mutant and FRT, mutant stocks are established, only one cross is required to generate flies with the potential to cells lack GAL80 and hence possess an active contain MARCM clones. Crossing these two stocks together and selecting against the balancer GAL4 that can activ ...
... tic recombination, homozygous mutant and FRT, mutant stocks are established, only one cross is required to generate flies with the potential to cells lack GAL80 and hence possess an active contain MARCM clones. Crossing these two stocks together and selecting against the balancer GAL4 that can activ ...
Chromosome intermingling—the physical basis of chromosome
... chromatin dynamics (24). Differentiation results in drastic changes to these properties (25–27) that are accomplished only within a few cell divisions. Since chromosomes can only move in a constrained fashion during interphase (28), the cell type-specific CT organization should accumulate progressiv ...
... chromatin dynamics (24). Differentiation results in drastic changes to these properties (25–27) that are accomplished only within a few cell divisions. Since chromosomes can only move in a constrained fashion during interphase (28), the cell type-specific CT organization should accumulate progressiv ...
PDF + SI - Development - The Company of Biologists
... and Hadjantonakis, 2007). Fate mapping experiments demonstrate that transient Eomes expression marks progenitors of the cardiovascular lineage, definitive endoderm (DE), node and midline (Costello et al., 2011). Transgenic and targeted deletion approaches have provided insight into cell type specifi ...
... and Hadjantonakis, 2007). Fate mapping experiments demonstrate that transient Eomes expression marks progenitors of the cardiovascular lineage, definitive endoderm (DE), node and midline (Costello et al., 2011). Transgenic and targeted deletion approaches have provided insight into cell type specifi ...
In-class assignment: Fukuda et al. (2016) paper
... 7. Xist and Tsix have antagonistic functions.Are the results in 5a consistent with this? Why or why not? Explain. The results in Fig 5a indicate that Xist and Tsix are regulated similarly by Oct4. When Oct4 is knocked out, both transcript levels drop, suggesting that Oct4 is a positive regulator of ...
... 7. Xist and Tsix have antagonistic functions.Are the results in 5a consistent with this? Why or why not? Explain. The results in Fig 5a indicate that Xist and Tsix are regulated similarly by Oct4. When Oct4 is knocked out, both transcript levels drop, suggesting that Oct4 is a positive regulator of ...
Identification of a Novel Point Mutation of Mouse Proto
... no mast cells were observed in Wads m/m skin tissues (Figure 1F). This result indicated that Wads-related c-kit mutation affects the development of the mast cell lineage. Lack of mature germ cell in Wads m/m mutants: The mating test suggested both Wads m/m males and females were infertile because no ...
... no mast cells were observed in Wads m/m skin tissues (Figure 1F). This result indicated that Wads-related c-kit mutation affects the development of the mast cell lineage. Lack of mature germ cell in Wads m/m mutants: The mating test suggested both Wads m/m males and females were infertile because no ...
The Mouse Immunoglobulin Heavy Chain V
... a productive VDJ rearrangement has been generated, further V to DJ recombination is prevented on the second allele, a process termed allelic exclusion, which in B cells ensures that each B cell expresses a monoclonal IgH (1). Ordered recombination is crucial for antigen receptor integrity, but key q ...
... a productive VDJ rearrangement has been generated, further V to DJ recombination is prevented on the second allele, a process termed allelic exclusion, which in B cells ensures that each B cell expresses a monoclonal IgH (1). Ordered recombination is crucial for antigen receptor integrity, but key q ...