DNA Base Sequence Homology in Rhizoctonia solani Kuihn: Inter
... (6), indicating genetic homogeneity among isolates within these groups. Hybridization between isolates of different AG was 30% or less (6,15). Ranges of DNA hybridization values varied for different AG, and lower levels of hybridization have confirmed lack of homogeneity among isolates within AG-1, ...
... (6), indicating genetic homogeneity among isolates within these groups. Hybridization between isolates of different AG was 30% or less (6,15). Ranges of DNA hybridization values varied for different AG, and lower levels of hybridization have confirmed lack of homogeneity among isolates within AG-1, ...
Supplementary Figure Legends (doc 34K)
... exons 49 to 57. NF1 exons named according to NCBI nomenclature (exons numbered 1-58) are represented by rectangles (not proportional to their size) at the genomic level and at the corresponding cDNA level. Exons 49 to 57 duplication was studied at the cDNA level. After reverse transcription of NF1 m ...
... exons 49 to 57. NF1 exons named according to NCBI nomenclature (exons numbered 1-58) are represented by rectangles (not proportional to their size) at the genomic level and at the corresponding cDNA level. Exons 49 to 57 duplication was studied at the cDNA level. After reverse transcription of NF1 m ...
International Journal of Antimicrobial Agents Mechanism of drug
... pathogen that causes gastroenteritis that is clinically indistinguishable from cholera [1–4]. Information regarding this human pathogen is limited because the enteritis caused by this organism is not as frequent as that caused by Vibrio cholerae [5]. However, in recent years it is being isolated wit ...
... pathogen that causes gastroenteritis that is clinically indistinguishable from cholera [1–4]. Information regarding this human pathogen is limited because the enteritis caused by this organism is not as frequent as that caused by Vibrio cholerae [5]. However, in recent years it is being isolated wit ...
Mechanistic Comparison of High-Fidelity and Error
... DNA polymerase fidelity has been measured in different ways, including (a) comparison of kinetic constants for Watson-Crick and mismatched nucleotide incorporation reactions and (b) measurement of misincorporation frequency when competing nucleotides are simultaneously present.8-10 Note that it has ...
... DNA polymerase fidelity has been measured in different ways, including (a) comparison of kinetic constants for Watson-Crick and mismatched nucleotide incorporation reactions and (b) measurement of misincorporation frequency when competing nucleotides are simultaneously present.8-10 Note that it has ...
DNA phosphorothioation inStreptomyces lividans: mutational
... sylated [1-3]. Altered or unusual bases in DNA molecules often have significant physiological implications, such as DNA replication control, gene regulation, or protection of the respective organisms from invasion by foreign DNA [4]. Page 1 of 8 (page number not for citation purposes) ...
... sylated [1-3]. Altered or unusual bases in DNA molecules often have significant physiological implications, such as DNA replication control, gene regulation, or protection of the respective organisms from invasion by foreign DNA [4]. Page 1 of 8 (page number not for citation purposes) ...
Misidentification of a genomovar of Burkholderia cepacia by recA
... patterns with most of bands—for example, being concentrated in the 300–500 bp range in the case of the HaeIII digestion pattern. In addition, not all bands are of equal intensity, so that weaker bands may be missed, leading to the identification of the wrong genomovar type. It may be argued that the ...
... patterns with most of bands—for example, being concentrated in the 300–500 bp range in the case of the HaeIII digestion pattern. In addition, not all bands are of equal intensity, so that weaker bands may be missed, leading to the identification of the wrong genomovar type. It may be argued that the ...
Application Note: Targeted sequencing and chromosomal haplotype
... using just one primer pair complementary to a short locusspecific sequence. TLA is a strategy to selectively amplify complete loci on the basis of crosslinking physically proximal sequences. Unlike other targeted sequencing methods, TLA works without prior detailed locus information, as one primer p ...
... using just one primer pair complementary to a short locusspecific sequence. TLA is a strategy to selectively amplify complete loci on the basis of crosslinking physically proximal sequences. Unlike other targeted sequencing methods, TLA works without prior detailed locus information, as one primer p ...
DNA How the Molecule of Heredity Carries, Replicates, and
... DNA stores information in the sequence of its bases. •Much of DNA’s sequence-specific information is accessible only when the double helix is unwound. •Proteins read the DNA sequence of nucleotides as the DNA helix unwinds. Proteins can either bind to a DNA sequence, or initiate the copying of it. ...
... DNA stores information in the sequence of its bases. •Much of DNA’s sequence-specific information is accessible only when the double helix is unwound. •Proteins read the DNA sequence of nucleotides as the DNA helix unwinds. Proteins can either bind to a DNA sequence, or initiate the copying of it. ...
e Study of RNA Polymerase Pausing by Optical Traps
... force on the bead. Further modifications included adding a more powerful and stable ND:YVO4 laser at 1064nm, removing the Wollaston prisms, and replacing the interferometric detection system with a position sensing diode. Custom software was written in LabView (National Instruments) to run the autom ...
... force on the bead. Further modifications included adding a more powerful and stable ND:YVO4 laser at 1064nm, removing the Wollaston prisms, and replacing the interferometric detection system with a position sensing diode. Custom software was written in LabView (National Instruments) to run the autom ...
Introduction and Preliminaries - Department of Computer and
... algorithms that may perform well in practice Approximation algorithms: Design a polynomialtime algorithm that is guaranteed to find near-optimal solutions (with a good approximation ratio) Heuristics: Fast algorithms that produce satisfactory solutions most of the time but without guarantee ...
... algorithms that may perform well in practice Approximation algorithms: Design a polynomialtime algorithm that is guaranteed to find near-optimal solutions (with a good approximation ratio) Heuristics: Fast algorithms that produce satisfactory solutions most of the time but without guarantee ...
Environmental Pollution
... This heap originates from the residuals of below-ground mining performed between 1905 and 1926 (Horst and Redel, 1977). The soil surrounding this heap supports the typical belt formation of plants dependent upon the salt concentration, with a surprisingly high richness of different halophytes. Soil ...
... This heap originates from the residuals of below-ground mining performed between 1905 and 1926 (Horst and Redel, 1977). The soil surrounding this heap supports the typical belt formation of plants dependent upon the salt concentration, with a surprisingly high richness of different halophytes. Soil ...
Computer-Aided DNA Base Calling from Forward and Reverse
... an electropherogram that is a 4-component time series made of the samples of the emissions measured by the 4 optical sensors. In principle, the DNA sequence can be obtained from the electropherogram by associating each dominant peak with the corresponding base type and by preserving the order of the ...
... an electropherogram that is a 4-component time series made of the samples of the emissions measured by the 4 optical sensors. In principle, the DNA sequence can be obtained from the electropherogram by associating each dominant peak with the corresponding base type and by preserving the order of the ...
Somatic MEN1 gene mutation does not contribute
... homo- or hemizygosity. In one case only we identified a possible point mutation in the promoter region. As the patient originated in South America, we cannot exclude a polymorphism. Unfortunately, peripheral blood was not available to discriminate between these two possibilities. Our results are con ...
... homo- or hemizygosity. In one case only we identified a possible point mutation in the promoter region. As the patient originated in South America, we cannot exclude a polymorphism. Unfortunately, peripheral blood was not available to discriminate between these two possibilities. Our results are con ...
Bioinformatics Dr. Víctor Treviño Pabellón Tec
... a tree is referred to as the tree length. The tree is also a bifurcating or binary tree, in that only two branches emanate from each node. Trees can have more than one branch emanating from a node if the events separating taxa are so close that they cannot be resolved, or to simplify the tree. The u ...
... a tree is referred to as the tree length. The tree is also a bifurcating or binary tree, in that only two branches emanate from each node. Trees can have more than one branch emanating from a node if the events separating taxa are so close that they cannot be resolved, or to simplify the tree. The u ...
The genomic landscape of meiotic crossovers and gene
... genotypes. Since all three parental lines are highly inbred, and hence homozygous throughout their genomes, the presence of heterozygous marker calls can only be explained by misaligned reads that result from unknown rearrangements or repeats. This is especially critical for Ler and Cvi, where genom ...
... genotypes. Since all three parental lines are highly inbred, and hence homozygous throughout their genomes, the presence of heterozygous marker calls can only be explained by misaligned reads that result from unknown rearrangements or repeats. This is especially critical for Ler and Cvi, where genom ...
Article A Distinct Mitochondrial Genome with DUI
... The doubly uniparental inheritance (DUI) system, described in more than 40 bivalve species, constitutes an exception to the commonly accepted rule of maternal mtDNA inheritance in animals. It is characterized by the existence of two distinct sex-associated mitochondrial genomes: The female (F-) mtDN ...
... The doubly uniparental inheritance (DUI) system, described in more than 40 bivalve species, constitutes an exception to the commonly accepted rule of maternal mtDNA inheritance in animals. It is characterized by the existence of two distinct sex-associated mitochondrial genomes: The female (F-) mtDN ...
Sequence and phylogenetic analysis of the gp200 protein of
... Fig. 5. The specificity and detection limit of the gp200-targeted PCR amplification using the primer set EC200-F3/R3. (A) The DNA extracted from blood samples of dogs infected by different rickettsia (Lane 1: E. canis, Lane 2: E. chaffeensis, Lane 3: E. ewingii, Lane 4: A. platys, Lane 5: B. canis v ...
... Fig. 5. The specificity and detection limit of the gp200-targeted PCR amplification using the primer set EC200-F3/R3. (A) The DNA extracted from blood samples of dogs infected by different rickettsia (Lane 1: E. canis, Lane 2: E. chaffeensis, Lane 3: E. ewingii, Lane 4: A. platys, Lane 5: B. canis v ...
Epigenetic changes in the estrogen receptor α gene
... fundamental issue in the study of sexual differentiation of the brain. Epigenetic mechanisms are emerging as important mediators for the maintenance of the hormonal effects (Keverne and Curley, 2008; McCarthy and Crews, 2008; Matsuda et al., 2012). DNA methylation is a well characterized epigenetic ...
... fundamental issue in the study of sexual differentiation of the brain. Epigenetic mechanisms are emerging as important mediators for the maintenance of the hormonal effects (Keverne and Curley, 2008; McCarthy and Crews, 2008; Matsuda et al., 2012). DNA methylation is a well characterized epigenetic ...
Gene regulation in three dimensions
... 1D view the genome is seen as a linear string of nucleotides, where one or more transcription factors (TFs) bind to transcription factor binding sites (TFBSs), and thereby regulate the expression of genes that are nearby in the linear genomic sequence. The 2D process can be described with DNA loopin ...
... 1D view the genome is seen as a linear string of nucleotides, where one or more transcription factors (TFs) bind to transcription factor binding sites (TFBSs), and thereby regulate the expression of genes that are nearby in the linear genomic sequence. The 2D process can be described with DNA loopin ...
What`s new - JSI medical systems
... Mutations/variations at those positons are not listed in the Variation/Mutation table. In case separated coverage is used and the coverage in one sense is below the Min coverage absolute, only the sense with the coverage above the Min coverage absolute will be regarded. For those mutations the colum ...
... Mutations/variations at those positons are not listed in the Variation/Mutation table. In case separated coverage is used and the coverage in one sense is below the Min coverage absolute, only the sense with the coverage above the Min coverage absolute will be regarded. For those mutations the colum ...
Supplementary Figures (doc 9746K)
... categories are junctions whose start and stop sites have been annotated in the Ensembl gene annotation. The only difference is that the new junction group uses different combinations of start and stop sites. The 3rd and 4th categories are junctions having new 3’ stop sites (acceptor site) or 5’ star ...
... categories are junctions whose start and stop sites have been annotated in the Ensembl gene annotation. The only difference is that the new junction group uses different combinations of start and stop sites. The 3rd and 4th categories are junctions having new 3’ stop sites (acceptor site) or 5’ star ...
Mutation, Mutagens, and DNA Repair
... every baby born in the United States has a blood sample tested for abnormal or missing genes or proteins. Early detection can allow the doctor to prescribe drugs or to place the baby on a specific diet in order to prevent or reduce the severity of a disease. Another type of testing, known as carrier ...
... every baby born in the United States has a blood sample tested for abnormal or missing genes or proteins. Early detection can allow the doctor to prescribe drugs or to place the baby on a specific diet in order to prevent or reduce the severity of a disease. Another type of testing, known as carrier ...
HERE - iGEM 2016
... a. Include two additional base-bairs to make a new codon of which the last position is the first T from the TTCG overhang, thus making a two-codon linker. Typically you would include GG, TC or AG as these will make Gly(GGU), Ser(UCU)or Ser(AGU), which are small amino acids that are less likely to in ...
... a. Include two additional base-bairs to make a new codon of which the last position is the first T from the TTCG overhang, thus making a two-codon linker. Typically you would include GG, TC or AG as these will make Gly(GGU), Ser(UCU)or Ser(AGU), which are small amino acids that are less likely to in ...
The Euglena gracilis chloroplast rpoB gene
... precipitation. Before the RNA was utilized for cDNA synthesis, the DNA was digested with RQ1 RNase-free DNase (Promega Biotechnology, Madison,WI), followed by phenol and chloroform extractions and ethanol precipitation as previously described. Synthetic oligodeoxynucleotides for cDNA synthesis and p ...
... precipitation. Before the RNA was utilized for cDNA synthesis, the DNA was digested with RQ1 RNase-free DNase (Promega Biotechnology, Madison,WI), followed by phenol and chloroform extractions and ethanol precipitation as previously described. Synthetic oligodeoxynucleotides for cDNA synthesis and p ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).