P-Element Transformation with period Locus DNA Restores
... transformed flies carried only one dose of transduced per+ DNA (also, some of the flies we tested carried more than one dose, but were still long-period; see Experimental Procedures). We do not have enough molecular information to interpret the apparently subnormal activity of the two rescuing DNA s ...
... transformed flies carried only one dose of transduced per+ DNA (also, some of the flies we tested carried more than one dose, but were still long-period; see Experimental Procedures). We do not have enough molecular information to interpret the apparently subnormal activity of the two rescuing DNA s ...
Curr. Microbiol. 42
... thuringiensis standard strains, as well as on 27 B. thuringiensis field isolates, which have previously been found positive to a pair of universal primers (Un7,8 for cry7 and cry8 groups) [2]. Among the standard strains, only B. thuringiensis subsp. dakota HD-511 and B. thuringiensis subsp. kumamoto ...
... thuringiensis standard strains, as well as on 27 B. thuringiensis field isolates, which have previously been found positive to a pair of universal primers (Un7,8 for cry7 and cry8 groups) [2]. Among the standard strains, only B. thuringiensis subsp. dakota HD-511 and B. thuringiensis subsp. kumamoto ...
PCR and the detection of pathogens in W and WW
... Wyn-Jones et al. (1995) compared PCR with cell culture for the detection of enteroviruses in river and seawater samples. They found that detection using PCR gave comparable results to cell culture in a much shorter time period and that a detection limit of 5 plaque forming units (pfu)/sample for ent ...
... Wyn-Jones et al. (1995) compared PCR with cell culture for the detection of enteroviruses in river and seawater samples. They found that detection using PCR gave comparable results to cell culture in a much shorter time period and that a detection limit of 5 plaque forming units (pfu)/sample for ent ...
Contribution of IKBKE and IFIH1 gene variants to SLE susceptibility
... (r2 ¼ 0.45–1.00; D0 ¼ 0.93–1.00). For IFIH1, strong LD was observed between the SNVs rs3747517 and rs1990760 (r2 ¼ 0.65; D0 ¼ 1.00), but not for the SNV rs78456138 (r2 ¼ 0.04–0.06; D0 ¼ 1.00). The results from pairwise conditional logistic regression analysis were in accordance with the correlation ...
... (r2 ¼ 0.45–1.00; D0 ¼ 0.93–1.00). For IFIH1, strong LD was observed between the SNVs rs3747517 and rs1990760 (r2 ¼ 0.65; D0 ¼ 1.00), but not for the SNV rs78456138 (r2 ¼ 0.04–0.06; D0 ¼ 1.00). The results from pairwise conditional logistic regression analysis were in accordance with the correlation ...
Construction of plant BAC libraries This document
... 6. Seal the wells containing ladders with melted LMP agarose. Allow the LMP agarose to solidify. 7. Remove the gel from the casting stand, and wipe any agarose off the bottom of the base plate. 8. Place the base plate and overlying gel in the BioRad CHEF Mapper apparatus. The unit should contain 2.5 ...
... 6. Seal the wells containing ladders with melted LMP agarose. Allow the LMP agarose to solidify. 7. Remove the gel from the casting stand, and wipe any agarose off the bottom of the base plate. 8. Place the base plate and overlying gel in the BioRad CHEF Mapper apparatus. The unit should contain 2.5 ...
Assembly of additional heterochromatin distinct from centromere
... gene flanked by 1.2 kb sequences of chicken -globin 5⬘HS4 region (cHS4) which is known to function as an insulator even in human cells (Recillas-Targa et al., 2002). The transient expression levels tested on these similar geo plasmid DNAs – that differ only in the promoter sequences – showed 10⫻ h ...
... gene flanked by 1.2 kb sequences of chicken -globin 5⬘HS4 region (cHS4) which is known to function as an insulator even in human cells (Recillas-Targa et al., 2002). The transient expression levels tested on these similar geo plasmid DNAs – that differ only in the promoter sequences – showed 10⫻ h ...
Complete Thyroxine-Binding Globulin (TBG) Deficiency Produced
... and a final extension at 72 C for 15 min. Automated fluorescence-based cycle sequencing (ABI, Perkin Elmer, Foster City, CA) was performed directly with the PCR products. The oligonucleotide primers used were the same as those used in the second PCR. ...
... and a final extension at 72 C for 15 min. Automated fluorescence-based cycle sequencing (ABI, Perkin Elmer, Foster City, CA) was performed directly with the PCR products. The oligonucleotide primers used were the same as those used in the second PCR. ...
Assaying … promoter activity
... Assaying … promoter activity To determine the activity of the … promoter in the wild type and mutant strains of AN12, we performed a transcriptional GUS assay. fusion assay in which the promoter from the …gene was fused to the coding sequence of the bglucuronidase reporter gene (gusA). ...
... Assaying … promoter activity To determine the activity of the … promoter in the wild type and mutant strains of AN12, we performed a transcriptional GUS assay. fusion assay in which the promoter from the …gene was fused to the coding sequence of the bglucuronidase reporter gene (gusA). ...
Determination of primary structure
... The BIOINFORMATICAL method requires information from chemical or physical, but only a limited amount of sequence. Example: a sequence of 6 AA is only possible as one of 206 possible hexa-peptide sequences (1 of 64 x 106). There are no more than 50,000 protein-coding genes with ≤400 AA on average. Th ...
... The BIOINFORMATICAL method requires information from chemical or physical, but only a limited amount of sequence. Example: a sequence of 6 AA is only possible as one of 206 possible hexa-peptide sequences (1 of 64 x 106). There are no more than 50,000 protein-coding genes with ≤400 AA on average. Th ...
Antisense Transcript and RNA Processing
... exceptions were spa19 and spa23, which maintained unusual heteroplasmic chloroplast genomes. One genome type, termed PSþ, conferred photosynthetic competence by contributing to the stability of atpB mRNA; the other, termed PS, was required for viability but could not produce stable atpB transcripts ...
... exceptions were spa19 and spa23, which maintained unusual heteroplasmic chloroplast genomes. One genome type, termed PSþ, conferred photosynthetic competence by contributing to the stability of atpB mRNA; the other, termed PS, was required for viability but could not produce stable atpB transcripts ...
Lysines 72, 80 and 213 and aspartic acid 210 of the
... polymerase followed by self-ligation. As a consequence, the EcoOlO9 (also Avail) site, which is located upstream of the lacR ATG start codon, is inactivated and two unique A vdU. sites flank the lacR codons 18-101 (Figure 1). Plasmid pNZ399 containing the lac promoter/operator region (Van Rooijen et ...
... polymerase followed by self-ligation. As a consequence, the EcoOlO9 (also Avail) site, which is located upstream of the lacR ATG start codon, is inactivated and two unique A vdU. sites flank the lacR codons 18-101 (Figure 1). Plasmid pNZ399 containing the lac promoter/operator region (Van Rooijen et ...
Global MicroRNA Amplification Kit
... of revealing the role miRNAs play in cellular development and disease. SBI has developed a simple and reliable method for preamplification of small RNAs for either qRT-PCR or miRNA microarray studies. There are estimated to be hundreds of distinct miRNAs in mammalian cells, not including the many ot ...
... of revealing the role miRNAs play in cellular development and disease. SBI has developed a simple and reliable method for preamplification of small RNAs for either qRT-PCR or miRNA microarray studies. There are estimated to be hundreds of distinct miRNAs in mammalian cells, not including the many ot ...
Case study of primary imatinib resistance and correlation of BCR
... the patient and healthy control, 112 bp ASOPCR product was observed only for the CML patient (Figure 3). It is inferred that mutation C1052T mutation is also involved in conferring imatinib resistance in this patient. Two mutations are responsible for primary imatinib resistance in the CML patient u ...
... the patient and healthy control, 112 bp ASOPCR product was observed only for the CML patient (Figure 3). It is inferred that mutation C1052T mutation is also involved in conferring imatinib resistance in this patient. Two mutations are responsible for primary imatinib resistance in the CML patient u ...
Supplementary document Trehalose/2
... al 1997), was 0.44±0.26. This value is in accordance with the previously reported yield of ...
... al 1997), was 0.44±0.26. This value is in accordance with the previously reported yield of ...
A P element-homologous sequence in the house fly, Musca domestica
... from M. domestica. The overall picture is one of reasonable conservation in the central portion of the P elements comprising exons 1 and 2, and considerable divergence in exons 0 and 3. It is unclear why the central portion of the P element has been more conserved over evolutionary time than regions ...
... from M. domestica. The overall picture is one of reasonable conservation in the central portion of the P elements comprising exons 1 and 2, and considerable divergence in exons 0 and 3. It is unclear why the central portion of the P element has been more conserved over evolutionary time than regions ...
Guanine-Plus-Cytosine Content of Rothia dentocaviosa
... than those published by Slack and Gerenscer (10) (51 mol% G+C for strain ATCC 17931T)and are far below the values in an earlier publication (4) (69.7 mol% G+C for the filamentous form of strain ATCC 17931T).The value for the clinical isolate was 52.9 mol% (range for four determinations, 52.0 to 53.3 ...
... than those published by Slack and Gerenscer (10) (51 mol% G+C for strain ATCC 17931T)and are far below the values in an earlier publication (4) (69.7 mol% G+C for the filamentous form of strain ATCC 17931T).The value for the clinical isolate was 52.9 mol% (range for four determinations, 52.0 to 53.3 ...
Multiplex in-vitro Detection using SERS
... The sensitive and specific analysis of biomolecules from complex mixtures is essential in the field of clinical diagnostics. The presence and progression of disease generally involves a multitude of different biomolecules; thus the detection of multiple events in tandem can reduce time and cost, as ...
... The sensitive and specific analysis of biomolecules from complex mixtures is essential in the field of clinical diagnostics. The presence and progression of disease generally involves a multitude of different biomolecules; thus the detection of multiple events in tandem can reduce time and cost, as ...
Extension Activity 1: Plasmid Mapping STUDENT MANU AL
... used in the DNA fingerprinting activity were constructed from the same pTZ18U plasmid parent but had different foreign fragments of DNA inserted into them. In the DNA fingerprinting exercise, only two restriction enzymes were used, but other enzymes could also have been used to cut these plasmids. T ...
... used in the DNA fingerprinting activity were constructed from the same pTZ18U plasmid parent but had different foreign fragments of DNA inserted into them. In the DNA fingerprinting exercise, only two restriction enzymes were used, but other enzymes could also have been used to cut these plasmids. T ...
DNA Base Sequence Homology in Rhizoctonia solani Kuihn: Inter
... (6), indicating genetic homogeneity among isolates within these groups. Hybridization between isolates of different AG was 30% or less (6,15). Ranges of DNA hybridization values varied for different AG, and lower levels of hybridization have confirmed lack of homogeneity among isolates within AG-1, ...
... (6), indicating genetic homogeneity among isolates within these groups. Hybridization between isolates of different AG was 30% or less (6,15). Ranges of DNA hybridization values varied for different AG, and lower levels of hybridization have confirmed lack of homogeneity among isolates within AG-1, ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).