Geminivirus Replication Origins Have a Modular
... Tomato golden mosaic virus (TGMV) and bean golden mosaic virus (BGMV) are closely related geminiviruses with bipartite genomes. The A and B DNA components of each virus have cis-acting sequences necessary for replication, and their A components encode trans-acting factors required for this process. ...
... Tomato golden mosaic virus (TGMV) and bean golden mosaic virus (BGMV) are closely related geminiviruses with bipartite genomes. The A and B DNA components of each virus have cis-acting sequences necessary for replication, and their A components encode trans-acting factors required for this process. ...
TILLING. Traditional Mutagenesis Meets
... Dissemination of TILLING technology to benefit plant research has been a major goal of our NSFfunded project. The process is sufficiently complex, both technically and logistically, that we decided to hold two-day workshops so that potential TILLING providers in the academic community can observe th ...
... Dissemination of TILLING technology to benefit plant research has been a major goal of our NSFfunded project. The process is sufficiently complex, both technically and logistically, that we decided to hold two-day workshops so that potential TILLING providers in the academic community can observe th ...
Chapter 1 Introduction
... Already in 1960 it was evident that the Y chromosome could vary considerably between individuals (Patau, 1960). At the London Conference on ‘The normal human karyotype’ in 1963, it became apparent that also the secondary constrictions near the centromeres of chromosomes 1, 9 and 16 could vary in siz ...
... Already in 1960 it was evident that the Y chromosome could vary considerably between individuals (Patau, 1960). At the London Conference on ‘The normal human karyotype’ in 1963, it became apparent that also the secondary constrictions near the centromeres of chromosomes 1, 9 and 16 could vary in siz ...
Immobilization_Mecha..
... performed using ImageJ.17 For each sample, the average intensity of five spots was taken, along with a background area next to each spot. The fluorescence signal for each spot was determined by normalization to the background intensity: signal = [spot intensity/background intensity] − 1. With this tec ...
... performed using ImageJ.17 For each sample, the average intensity of five spots was taken, along with a background area next to each spot. The fluorescence signal for each spot was determined by normalization to the background intensity: signal = [spot intensity/background intensity] − 1. With this tec ...
PDF - Fred Hutchinson Cancer Research Center
... Most previous applications of deep mutational scanning have examined single-nucleotide mutations to genes, because such mutations can easily be generated by error-prone polymerase chain reaction (PCR) or other nucleotide-level mutagenesis techniques. However, many amino acid mutations are not access ...
... Most previous applications of deep mutational scanning have examined single-nucleotide mutations to genes, because such mutations can easily be generated by error-prone polymerase chain reaction (PCR) or other nucleotide-level mutagenesis techniques. However, many amino acid mutations are not access ...
Comparative Genomic Hybridization in Chronic B
... equipped with a cooled CCD camera (Photometrics, Tucson, AZ). FITC and rhodamine images of the tumor and control DNA were used for quantitative analysis. Ratio profiles of each individual chromosome were calculated using a dedicated software?' For each case, the mean ratio profiles of between 4 and ...
... equipped with a cooled CCD camera (Photometrics, Tucson, AZ). FITC and rhodamine images of the tumor and control DNA were used for quantitative analysis. Ratio profiles of each individual chromosome were calculated using a dedicated software?' For each case, the mean ratio profiles of between 4 and ...
Analysis and nucleotide sequence of an origin of DNA replication in
... pWH1266 and transformants were scored on LB plates containing 100/~gAp/ml. Competency is observed from the early log phase to the stationary phase. A maximum of competency is found in the late log phase of growth with about 6 × l0 s transformants per 500 ng of plasmid DNA. This procedure is slightly ...
... pWH1266 and transformants were scored on LB plates containing 100/~gAp/ml. Competency is observed from the early log phase to the stationary phase. A maximum of competency is found in the late log phase of growth with about 6 × l0 s transformants per 500 ng of plasmid DNA. This procedure is slightly ...
Insights into Protein–DNA Interactions through Structure
... defined across the two node sets. A contact in the bipartite PDG is defined when a side chain of an amino acid interacts with the nucleotide. The interactions of the amino acid with the nucleotide can be considered at different levels: with the phosphate (p), deoxyribose sugar (S) or base (B) compon ...
... defined across the two node sets. A contact in the bipartite PDG is defined when a side chain of an amino acid interacts with the nucleotide. The interactions of the amino acid with the nucleotide can be considered at different levels: with the phosphate (p), deoxyribose sugar (S) or base (B) compon ...
A natural chimeric yeast containing genetic material from three species
... redissolved DNA was restricted with XbaI and the resulting DNA fragments were purified from agarose using Bio-Rad Prep-A-Gene purification matrix. These fragments were ligated into pUC19 vectors that had been linearized with XbaI and treated with calf intestine alkaline phosphatase. The resulting pl ...
... redissolved DNA was restricted with XbaI and the resulting DNA fragments were purified from agarose using Bio-Rad Prep-A-Gene purification matrix. These fragments were ligated into pUC19 vectors that had been linearized with XbaI and treated with calf intestine alkaline phosphatase. The resulting pl ...
Molecular Genetic Testing For BRAF Mutations
... TaqMan® Mutation Detection Assay to detect the V600E mutation in exon 15 of BRAF in tumor (somatic) cells. The sensitivity of the TaqMan assay is ~0.1% mutant DNA in a wild-type background. Poor DNA quality, insufficient DNA quantity or the presence of PCR inhibitors can result in uninterpretable or ...
... TaqMan® Mutation Detection Assay to detect the V600E mutation in exon 15 of BRAF in tumor (somatic) cells. The sensitivity of the TaqMan assay is ~0.1% mutant DNA in a wild-type background. Poor DNA quality, insufficient DNA quantity or the presence of PCR inhibitors can result in uninterpretable or ...
Multifractal characterisation of length sequences of coding and
... in DNA sequences at this level. Li et al. [2,3] found that the spectral density of a DNA sequence containing mostly introns shows 1=f behaviour, which indicates the presence of LRC. The correlation properties of coding and noncoding DNA sequences were also studied by Peng et al. [4] in their fracta ...
... in DNA sequences at this level. Li et al. [2,3] found that the spectral density of a DNA sequence containing mostly introns shows 1=f behaviour, which indicates the presence of LRC. The correlation properties of coding and noncoding DNA sequences were also studied by Peng et al. [4] in their fracta ...
- SciTech Connect
... interfering RNAs (siRNAs) – for example, endogenous siRNAs (endo-siRNAs) in the mouse7,8 – and PIWI-interacting RNAs (piRNAs).7,8 The importance of maternally supplied RNA can be illustrated by studies of anterior– posterior axis formation in Drosophila melanogaster embryos. Their body axis is speci ...
... interfering RNAs (siRNAs) – for example, endogenous siRNAs (endo-siRNAs) in the mouse7,8 – and PIWI-interacting RNAs (piRNAs).7,8 The importance of maternally supplied RNA can be illustrated by studies of anterior– posterior axis formation in Drosophila melanogaster embryos. Their body axis is speci ...
functional analysis of chromatin assembly genes in tetrahymena
... The basic structural unit of chromatin is the nucleosome composed of ~147 base pairs of DNA wrapped around an octamer of histone proteins. Post-translational modifications such as histone acetylation or the substitution of histone variants in place of core histones have been implicated in various ch ...
... The basic structural unit of chromatin is the nucleosome composed of ~147 base pairs of DNA wrapped around an octamer of histone proteins. Post-translational modifications such as histone acetylation or the substitution of histone variants in place of core histones have been implicated in various ch ...
The use of the twin model to investigate the genetics and
... over environment in for the appearance of naevi. In 1991, almost seventy years after the reports by Siemens, a UK research group published the results of the study examining the concordance of naevus counts in 23 MZ and 22 DZ twin pairs.7 This study demonstrated a higher concordance in the number of ...
... over environment in for the appearance of naevi. In 1991, almost seventy years after the reports by Siemens, a UK research group published the results of the study examining the concordance of naevus counts in 23 MZ and 22 DZ twin pairs.7 This study demonstrated a higher concordance in the number of ...
insulin history
... recombinant insulin analog, Humalog (two amino acids are changed, which increases the ability of the body to use the recombinant insulin). Since the introduction of Humulin, many more recombinant protein drugs have been developed, including human growth hormone, erythropoietin, and parathyroid hormo ...
... recombinant insulin analog, Humalog (two amino acids are changed, which increases the ability of the body to use the recombinant insulin). Since the introduction of Humulin, many more recombinant protein drugs have been developed, including human growth hormone, erythropoietin, and parathyroid hormo ...
Molecular Identification of Nematodes Manual
... supply company or even a hardware store. There is no reason to buy over-priced mineral oil from some molecular biology company. The mineral oil does not need to be autoclaved to sterilize, but a negative control needs to be run on it.) *A negative control is the 40.0ul of master mix with 10.0ul of s ...
... supply company or even a hardware store. There is no reason to buy over-priced mineral oil from some molecular biology company. The mineral oil does not need to be autoclaved to sterilize, but a negative control needs to be run on it.) *A negative control is the 40.0ul of master mix with 10.0ul of s ...
Semiconservative Replication in the Quasispecies Model
... b̄L−i+1 ⇒ bi = b̄i , which is impossible. Therefore, σ and σ̄ must be equal at an even number of sites, hence lI must be odd for odd L and even for even L. Suppose L is odd, so L = 2l+1, and consider some lI = 2k + 1. We have complete freedom to choose b1 , . . . , bl+1 . We automatically have bl+1 ...
... b̄L−i+1 ⇒ bi = b̄i , which is impossible. Therefore, σ and σ̄ must be equal at an even number of sites, hence lI must be odd for odd L and even for even L. Suppose L is odd, so L = 2l+1, and consider some lI = 2k + 1. We have complete freedom to choose b1 , . . . , bl+1 . We automatically have bl+1 ...
The nucleotide sequence of Saccharomyces cerevisiae
... telomere shows good homology with the telomeres of several other chromosomes. There are also several other internal chromosomal regions with long-range homology to other chromosomes. The largest of these is an area common to chromosomes IX and XIV, occurring at 89,233–186,363 and 478,568–616,076, re ...
... telomere shows good homology with the telomeres of several other chromosomes. There are also several other internal chromosomal regions with long-range homology to other chromosomes. The largest of these is an area common to chromosomes IX and XIV, occurring at 89,233–186,363 and 478,568–616,076, re ...
Intelligent Icons: Integrating Lite-Weight Data Mining
... One could apply this simple mapping to a set of DNA sequences corresponding to different species and examine the icons in a file browser. Unsurprisingly however (and unfortunately for human vanity) there is very little difference between the icons obtained in this way for most mammals. In an attempt ...
... One could apply this simple mapping to a set of DNA sequences corresponding to different species and examine the icons in a file browser. Unsurprisingly however (and unfortunately for human vanity) there is very little difference between the icons obtained in this way for most mammals. In an attempt ...
CHAPTER 14 DNA applications in society
... glands from cadavers were required. Each batch of hGH was derived from a pool of pituitary glands from about 16 000 cadavers. During a year, patients received their hGH injections from two or three different batches. (A batch is a quantity of a medicine or drug that is manufactured under specific c ...
... glands from cadavers were required. Each batch of hGH was derived from a pool of pituitary glands from about 16 000 cadavers. During a year, patients received their hGH injections from two or three different batches. (A batch is a quantity of a medicine or drug that is manufactured under specific c ...
Two major types of JC virus defined in progressive multifocal
... Grace S. Auit* and Gerald L. Stoner Laboratory of Experimental Neuropathology, National Institute o f Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, U.S.A. ...
... Grace S. Auit* and Gerald L. Stoner Laboratory of Experimental Neuropathology, National Institute o f Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, U.S.A. ...
DNA breathing dynamics distinguish binding from nonbinding
... All fragments contain the flanking sequence (CCT) on both ends to minimize end wobbling. The gel shift results are consistent between three independent experiments. The gel shift reactions are conducted at 37 C. RESULTS LMD simulations distinguish true YY1 binding from nonbinding sites in the human ...
... All fragments contain the flanking sequence (CCT) on both ends to minimize end wobbling. The gel shift results are consistent between three independent experiments. The gel shift reactions are conducted at 37 C. RESULTS LMD simulations distinguish true YY1 binding from nonbinding sites in the human ...
A New Method for Estimating the Risk Ratio in Studies Using Case
... estimate the variance of the new estimator. The simplicity of the new estimator and its variance makes the new method appealing. When genotypic information from only one parent is available, Schaid and Sommer (5) proposed an estimation method for the risk ratio assuming both Hardy-Weinberg equilibri ...
... estimate the variance of the new estimator. The simplicity of the new estimator and its variance makes the new method appealing. When genotypic information from only one parent is available, Schaid and Sommer (5) proposed an estimation method for the risk ratio assuming both Hardy-Weinberg equilibri ...
Highly precise and developmentally programmed genome
... examination of cleaved IES ends has led to a mechanistic model, in which two DSBs, one at each end, initiate IES excision [11]. Genetic evidence for a crosstalk between ends before DNA cleavage further supported the view that IES ends are recognized or cleaved in a concerted manner [13]. It was prop ...
... examination of cleaved IES ends has led to a mechanistic model, in which two DSBs, one at each end, initiate IES excision [11]. Genetic evidence for a crosstalk between ends before DNA cleavage further supported the view that IES ends are recognized or cleaved in a concerted manner [13]. It was prop ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).