Mechanisms of fast and stringent search in homologous pairing of
... PH, and shear force, suggesting that it may serve as the ‘default’ mode of chromosome pairing in vivo [2]. Various models have been proposed to explain the homology-dependent attraction between dsDNA molecules [9–11], many of which attribute this interaction to hydrophobic forces or electrostatics. ...
... PH, and shear force, suggesting that it may serve as the ‘default’ mode of chromosome pairing in vivo [2]. Various models have been proposed to explain the homology-dependent attraction between dsDNA molecules [9–11], many of which attribute this interaction to hydrophobic forces or electrostatics. ...
LEADING ARTICLE Concurrent disruption of p16INK4a and
... inhibiting phosphorylation of the retinoblastoma protein (Rb), while ARF prevents MDM2-mediated degradation of p53. By using a panel of PCR-based methods, we have examined the status of the p16INK4a, ARF and p53 genes in 123 cases of nonHodgkin’s lymphoma (NHL) at diagnosis. Alterations of one or mo ...
... inhibiting phosphorylation of the retinoblastoma protein (Rb), while ARF prevents MDM2-mediated degradation of p53. By using a panel of PCR-based methods, we have examined the status of the p16INK4a, ARF and p53 genes in 123 cases of nonHodgkin’s lymphoma (NHL) at diagnosis. Alterations of one or mo ...
Harvey ras (H-ras) Point Mutations Are Induced by 4
... Since the 4NQO-induced lesions closely resembled human head and neck cancer, the incidence of H-ras mutations was investigated in 4NQO-induced oral cavity tumors using highly sensitive molecular techniques. H-ras point mutations were detected in approximately 60% of tumor tissues. Most remarkably, l ...
... Since the 4NQO-induced lesions closely resembled human head and neck cancer, the incidence of H-ras mutations was investigated in 4NQO-induced oral cavity tumors using highly sensitive molecular techniques. H-ras point mutations were detected in approximately 60% of tumor tissues. Most remarkably, l ...
Amiito acid sequence of the testosterone
... polyadenylation sites (9). This was determined by SI nuclease mapping. Additional SI nuclease mapping has now established that a third polyadenylation site is utilized to produce the 1350 nt mRNA. An SI nuclease protection assay was performed with the 412 base long 3' end-labeled Hinf I fragment dia ...
... polyadenylation sites (9). This was determined by SI nuclease mapping. Additional SI nuclease mapping has now established that a third polyadenylation site is utilized to produce the 1350 nt mRNA. An SI nuclease protection assay was performed with the 412 base long 3' end-labeled Hinf I fragment dia ...
Mapping of the Recessive White Locus and
... Game and the White Plymouth Rock. Five pairs of primers were produced based on the chicken tyrosinase gene information (UCSC Genome Browser on Chicken; alignment of AB023291 and chromosome 1:179545508 to 179595094; Table 1). The PCR was performed in a total volume of 15 L containing 20 ng of genomi ...
... Game and the White Plymouth Rock. Five pairs of primers were produced based on the chicken tyrosinase gene information (UCSC Genome Browser on Chicken; alignment of AB023291 and chromosome 1:179545508 to 179595094; Table 1). The PCR was performed in a total volume of 15 L containing 20 ng of genomi ...
Stage-specific expressions of four different ribonuclease H genes in
... The human pathogen of the genus Leishmania cause worldwide morbidity and infection of visceral organs by some species may be lethal. Lack of rational chemotherapy against these pathogens dictates the study of differential biochemistry and molecular biology of the parasite as compared to its human ho ...
... The human pathogen of the genus Leishmania cause worldwide morbidity and infection of visceral organs by some species may be lethal. Lack of rational chemotherapy against these pathogens dictates the study of differential biochemistry and molecular biology of the parasite as compared to its human ho ...
53 - Lab Times
... be in danger of extinction when it comes to sequencing entire genomes like there’s no tomorrow. Instead of cloning single genes, as in the “old days”, many of today’s molecular biologists clone large sets of genes to determine their function. ...
... be in danger of extinction when it comes to sequencing entire genomes like there’s no tomorrow. Instead of cloning single genes, as in the “old days”, many of today’s molecular biologists clone large sets of genes to determine their function. ...
Handbook on SMA genetics_final_051209
... intragenic SMN1 point mutations. Exonic regions must be individually amplified; therefore, sequence analysis does not detect exonic deletions or duplications. ...
... intragenic SMN1 point mutations. Exonic regions must be individually amplified; therefore, sequence analysis does not detect exonic deletions or duplications. ...
FUNCTION IN PHYSARUM POLYCEPHALUM mitochondria of
... FIGURE 2 Electron micrographs of surface-spread and shadowed mitochondrion (Fig. 2 a) and a portion of phosphotungstic acid- (Fig. 2 b) or uranyl acetate-stained cristae (Fig. 2 c) in a control preparation. In Fig. 2 a, several tubular cristae and a centrally located electron-dense rodlike body can ...
... FIGURE 2 Electron micrographs of surface-spread and shadowed mitochondrion (Fig. 2 a) and a portion of phosphotungstic acid- (Fig. 2 b) or uranyl acetate-stained cristae (Fig. 2 c) in a control preparation. In Fig. 2 a, several tubular cristae and a centrally located electron-dense rodlike body can ...
Molecular Characterization of a Powdery Mildew Resistance Gene
... resistance genes, only Pm3 was identified on chromosome 1AS (12). A total of 10 alleles (Pm3a to Pm3j) have been reported at this locus. To determine if the resistance gene in Suwon 92 resides at the same locus as Pm3, 10 primer pairs, designed based on the cloned Pm3b sequence, were screened using ...
... resistance genes, only Pm3 was identified on chromosome 1AS (12). A total of 10 alleles (Pm3a to Pm3j) have been reported at this locus. To determine if the resistance gene in Suwon 92 resides at the same locus as Pm3, 10 primer pairs, designed based on the cloned Pm3b sequence, were screened using ...
Identification, cloning and sequence determination of genes specifying hexokinase A and B from yeast.
... this tryptic peptide had been tentatively assigned as spanning residues 385-396 in the approximated structure derived from x-ray crystallography (5), the 5' and 3' ends of the HKB structural gene were calculated to be roughly 1170 nucleotides upstream and 210 downstream from the PstI site. Since a n ...
... this tryptic peptide had been tentatively assigned as spanning residues 385-396 in the approximated structure derived from x-ray crystallography (5), the 5' and 3' ends of the HKB structural gene were calculated to be roughly 1170 nucleotides upstream and 210 downstream from the PstI site. Since a n ...
The Role of the C-terminal Tail of the Ribosomal Protein S13 in Pr
... modified S13 CTD sequence, a stop codon, an E. coli SD sequence for translation of amp resistance gene, and short sequence complementary to the amp resistance gene in pND707. The reverse primer was the same; each forward primer had a different S13 CTD sequence before its stop codon. ...
... modified S13 CTD sequence, a stop codon, an E. coli SD sequence for translation of amp resistance gene, and short sequence complementary to the amp resistance gene in pND707. The reverse primer was the same; each forward primer had a different S13 CTD sequence before its stop codon. ...
Chapter 8
... • Transcription proceeds in the 5' 3' direction; only one of the two DNA strands is transcribed • Transcription stops when it reaches the terminator sequence on DNA ...
... • Transcription proceeds in the 5' 3' direction; only one of the two DNA strands is transcribed • Transcription stops when it reaches the terminator sequence on DNA ...
Sequence Alignment
... BLOSUM matrices with higher numbers and PAM matrices with low numbers are designed for comparisons of closely related sequences. BLOSUM matrices with low numbers and PAM matrices with high numbers are designed for comparisons of distantly related proteins. ...
... BLOSUM matrices with higher numbers and PAM matrices with low numbers are designed for comparisons of closely related sequences. BLOSUM matrices with low numbers and PAM matrices with high numbers are designed for comparisons of distantly related proteins. ...
Isolation and characterization of a repeated sequence (RPS1) of
... chromosome bands usually assigned by pTK2-9-1 or ERG1 1, it also weakly hybridized to almost all of the other chromosomes (Fig. lb). In strains NUM215, NUM47, NUMll4 and NUM812, some chromosomes other than chromosome 6 strongly hybridized to the RPSl probe. It is worth noting that only chromosome 4, ...
... chromosome bands usually assigned by pTK2-9-1 or ERG1 1, it also weakly hybridized to almost all of the other chromosomes (Fig. lb). In strains NUM215, NUM47, NUMll4 and NUM812, some chromosomes other than chromosome 6 strongly hybridized to the RPSl probe. It is worth noting that only chromosome 4, ...
Technique ChIC and ChEC: Genomic Mapping of Chromatin Proteins
... sites was inserted (Ishii and Laemmli, 2003). Cleavage sites were mapped by Southern blotting and indirect end labeling, in which a DNA fragment abutting a restriction enzyme cleavage site is used as a hybridization probe. This procedure maps DNA cleavage cuts relative to this restriction site (see ...
... sites was inserted (Ishii and Laemmli, 2003). Cleavage sites were mapped by Southern blotting and indirect end labeling, in which a DNA fragment abutting a restriction enzyme cleavage site is used as a hybridization probe. This procedure maps DNA cleavage cuts relative to this restriction site (see ...
Myriad--Ambry -- Final Version of Ambry Preliminary Injunction
... million dollars to implement this discovery and create a molecular diagnostic test for hereditary breast and ovarian cancer related to the BRCA1 and BRCA2 genes that has revolutionized patient care and provided medical diagnosis and treatment options never thought possible. This investment has yield ...
... million dollars to implement this discovery and create a molecular diagnostic test for hereditary breast and ovarian cancer related to the BRCA1 and BRCA2 genes that has revolutionized patient care and provided medical diagnosis and treatment options never thought possible. This investment has yield ...
A GENETIC LINKAGE MAP OF Phycomyces blakesleeanus
... this research the genome (version 1.1) was made up of 491 fragments. The genetic linkage map shows the arrangement of genes and genetic markers along the chromosomes as calculated by the frequency with which they are co-inherited together. The map can also provide information about the number of ch ...
... this research the genome (version 1.1) was made up of 491 fragments. The genetic linkage map shows the arrangement of genes and genetic markers along the chromosomes as calculated by the frequency with which they are co-inherited together. The map can also provide information about the number of ch ...
background of the invention
... reaction conducted at room temperature (without requiring a heating system) or a constant temperature (such as 37℃) to replace the conventional PCR technology that requires a precise control of three temperature cycles, and the isothermal device is more suitable for precision electrical measurements ...
... reaction conducted at room temperature (without requiring a heating system) or a constant temperature (such as 37℃) to replace the conventional PCR technology that requires a precise control of three temperature cycles, and the isothermal device is more suitable for precision electrical measurements ...
Thermodynamic analysis of DNA binding by a Bacillus single
... Department of Bioscience Technologies Faculty Papers by an authorized administrator of the Jefferson Digital Commons. For more information, please ...
... Department of Bioscience Technologies Faculty Papers by an authorized administrator of the Jefferson Digital Commons. For more information, please ...
Developing expressed sequence tag libraries and
... (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. ...
... (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. ...
Test Info Sheet
... 4. Prenatal diagnosis for known familial mutation(s) in at-risk pregnancies Methods: Using genomic DNA, coding exons and flanking splice junctions of the genes on this panel are enriched using a proprietary targeted capture method developed by GeneDx. The products are sequenced on an Illumina instru ...
... 4. Prenatal diagnosis for known familial mutation(s) in at-risk pregnancies Methods: Using genomic DNA, coding exons and flanking splice junctions of the genes on this panel are enriched using a proprietary targeted capture method developed by GeneDx. The products are sequenced on an Illumina instru ...
Transcriptional analysis of the gene for glutamine synthetase II and
... Two S. coelicolor DNA fragments carrying glnII were isolated from cosmids constructed by Redenbach et al. (1996) after Southern hybridization with a glnII probe from S. viridochromogenes (data not shown): a 3.2-kb BglII fragment from cosmid C118 and a 2.7-kb BamHISacI fragment from cosmid 10B7. Each ...
... Two S. coelicolor DNA fragments carrying glnII were isolated from cosmids constructed by Redenbach et al. (1996) after Southern hybridization with a glnII probe from S. viridochromogenes (data not shown): a 3.2-kb BglII fragment from cosmid C118 and a 2.7-kb BamHISacI fragment from cosmid 10B7. Each ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).