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Development of Co-Dominant Amplified Polymorphic Sequence
Development of Co-Dominant Amplified Polymorphic Sequence

... areas of rice cultivation and is a primary biotic source of yield loss worldwide (15). The utilization of resistant cultivars is the most cost-effective means to mitigate disease losses. However, monogenic resistance commonly is rendered ineffective if frequency of corresponding virulence alleles in ...
Youngson and Whitelaw, 2008
Youngson and Whitelaw, 2008

... the establishment of epigenetic state can be influenced by environmental factors (33, 40, 129). To ensure the totipotency of the zygote and to prevent perpetuation of abnormal epigenetic states, most gene regulatory, i.e., epigenetic, information is not transferred between generations. Several mechan ...
Microbiology
Microbiology

... UDPglucose 4-epimerase (GalE) activity was missing in this mutant strain. DNA hybridization studies showed that the GRHZ-50 mutant DNA has homologous sequences within the different biovars of Rhizobium leguminosarum. However, no DNA homology to GRHZ-14 altered DNA was found in those rhizobial strain ...
Surface polysaccharide mutants of Rhizobium sp. (Acacia) strain
Surface polysaccharide mutants of Rhizobium sp. (Acacia) strain

DNA and Genes - Buckeye Valley
DNA and Genes - Buckeye Valley

Diagnostic protocol for
Diagnostic protocol for

... al. 1998). Nested PCR, immunocapture and colorimetric detection of nested PCR products for direct and sensitive detection of Xcc in plants have also been developed (Hartung et al. 1993). ...
Specialized Transduction
Specialized Transduction

... subsequently in coliphage P1 (44), an unrelated temperate coliphage, has no such limitation (see chapter 130 in this volume). There is a second important distinction between specialized and generalized transduction. The limited set of host genes that λ naturally transduces can be stably incorporated ...
Molecular characterization of MHC class II in a nonmodel anuran
Molecular characterization of MHC class II in a nonmodel anuran

... Sato et al. 1993; D50035, Kobari et al. 1995) and from two urodelan species, the tiger salamander (A. tigrinum; DQ071905-7, DQ071909-12 Bos and DeWoody 2005) and the axolotl (A. mexicana; AF209117 Laurens et al. 2001), to design sets of PCR primers for Bombina (Table 1). Primer pair 4 successfully a ...
Requirements for Human Medical Genome
Requirements for Human Medical Genome

... Medical testing by genomic methods shares many ethical, legal and social issues with other forms of clinical investigation. Existing issues of informed consent, incidental findings, the right not to know, family studies and re-contacting are potentially magnified due to the volume of information tha ...
Transcripts of the MHM region on the chicken Z chromosome
Transcripts of the MHM region on the chicken Z chromosome

... of the CpG dinucleotides of this region are extensively methylated on the two Z chromosomes in the male but much less methylated on the single Z chromosome in the female. The state of methylation of the MHM region is established after fertilization by about the 1-day embryonic stage. The MHM region ...
Fibrinogen Bern I: Substitution y 337 Asn + Lys Is
Fibrinogen Bern I: Substitution y 337 Asn + Lys Is

... bands at the same migration distance, corresponding to Lys (AAC + AAA). amino acid substitution y 337 Asn The purified double-stranded PCR-derived fragment was cloned and subjected to sequence analysis. Normal and mutated alleles of fibrinogen Bern I were obtained, showing a G + T transition in the ...
Spring 1997 - University of Idaho
Spring 1997 - University of Idaho

Lack of expression of XIST from a small ring X chromosome
Lack of expression of XIST from a small ring X chromosome

... ring (X) chromosome based on the loss of paternal alleles. (B) Sequencing of DNA amplified from the patient for the promoter of the XIST gene showed a base change as marked by the vertical arrow. A primer, PM2, was designed with a sequence change (G as shown in large arrow rather than A) that would ...
SNPs and Haplotypes
SNPs and Haplotypes

... • It’s sometimes possible to correlate a SNP with a particular trait or disease – This is known as association genetics ...
R4, a non-LTR retrotransposon specific to the
R4, a non-LTR retrotransposon specific to the

... extend only a few base pairs along the DNA, thus they insert at numerous locations throughout the host genome. When these elements fall within or near transcription units they can cause significant detrimental effects on the host. Less well appreciated is the risk that this random method of insertio ...
Separation of DNA Restriction Fragments by Ion
Separation of DNA Restriction Fragments by Ion

... plays a central role in DNA research. However, when used for preparative purposes electrophoresis has two particularly serious drawbacks: (A) Extraction of the purified material out of the gel is tedious and difficult to accomplish with high yields (1). (B) The purified DNA is often contaminated wit ...
the association of chloroplast dna with photosynthetic membrane
the association of chloroplast dna with photosynthetic membrane

... in the electron microscope they appear as in Figs. 6-8. They are bound by a membrane to which adhere varying numbers of grana lamellae. The number of grana lamellae seen in a single section will vary according to the section plane. The grana are partially disrupted due to the isolation in the 3-5 mM ...
Ancient DNA Laboratory Guidelines
Ancient DNA Laboratory Guidelines

... aDNA Laboratory, simply remove the trash and place it in the hallway outside the room. This should be done every day that the aDNA Laboratory is used, even if projects require the use of the aDNA Laboratory over several days. Publishable aDNA Laboratory Results “Ancient” DNA studies over the past f ...
"big IB objectives"-use the blank paper technique
"big IB objectives"-use the blank paper technique

... 3.4.1 – explain DNA replication in terms of unwinding the double helix and separation of the strands by helicase, followed by formation of the new complementary strands by DNA polymerase 3.4.2 – explain the significance of complementary base pairing in the conservation of the base sequence of DNA 3 ...
ACMG Standards and Guidelines for constitutional cytogenomic
ACMG Standards and Guidelines for constitutional cytogenomic

... detect. This should include both autosomal and sex chromosome abnormalities as duplications and deletions on the sex chromosomes may behave differently in each sex. Furthermore, blinding the evaluators to the expected abnormalities has the ­additional benefit of validating the settings, evaluation o ...
DNA MUTATION, REPAIR, AND TRANSPOSITION
DNA MUTATION, REPAIR, AND TRANSPOSITION

... their ends and at least one gene in the middle. The transposons are also similar in that they generate direct repeats during their insertion at target sites. EXERCISES AND PROBLEMS 22. UV light causes pyrimidine dimers, the most common of which are thymine– thymine dimers. Therefore the DNA molecule ...
Phylogenetic analysis of members of the genus Rickettsia using the
Phylogenetic analysis of members of the genus Rickettsia using the

... Type 377-36cm WTR ; Tebu) and electrophoresis was performed with the ABI PRISM 377 DNA sequencer (Perkin Elmer). The same primers as those used for PCR amplification were used for the sequencing reactions. Primers 120H-4442 and 120-4489 were used to confirm the sequence of the 3h end of the gene of ...
Charge Transport in DNA - Insights from
Charge Transport in DNA - Insights from

... of nucleobases on time scales larger than nanoseconds, dramatic enhancements in computational efficiency have to be made. In the final part of this work, one possible approach to this is developed - a parametrized model to simulate the CT in DNA. Not only will this model be able to describe the CT in ...
Binding of Hoechst with nucleic acids using fluorescence spectroscopy
Binding of Hoechst with nucleic acids using fluorescence spectroscopy

... Full understanding of the molecular mechanisms of action of heterocyclic antibiotics at low concentrations on DNA and RNA can optimize the use of these compounds and their analogues as medical drugs. Hoechst dyes 33342 and 33258 are closely related bisbenzimides. Hoechst 33258 is 2’-(4-hydroxyphenyl ...
ANALYSIS OF MULTIPLE RESTRICTION FRAGMENT LENGTH
ANALYSIS OF MULTIPLE RESTRICTION FRAGMENT LENGTH

... fragments in the FF individuals; therefore, an allelic Sac I fragment could not be identified (Fig . 4) . Sac I also generated a CRI-1 +, CRI-4'}, 7 .9-kb restriction fragment that was present only in the DNA of the four FF donors and two FS donors who exhibited the CRI-1+, GBIR1+, 14 .5-kb Bam HI f ...
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Bisulfite sequencing



Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).
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