Synapsis-Mediated Fusion of Free DNA Ends Forms Inverted Dimer Plasmids in Yeast.
... transformants, not shown) reveals a first order concentrationdependence, while double selection for Ura+ Leu+ transformants results in a second order dependence. These outcomes are expectedon the basis of a unimolecular requirement for the transformation event. On the other hand, with the plasmid DN ...
... transformants, not shown) reveals a first order concentrationdependence, while double selection for Ura+ Leu+ transformants results in a second order dependence. These outcomes are expectedon the basis of a unimolecular requirement for the transformation event. On the other hand, with the plasmid DN ...
Structure, expression and chromosomal location of the Oct
... the relevant parental t-haplotypes. This lack of polymorphism was not unexpected because all t-haplotypes are generally believed to be descended from a single ancestral chromosome. Another approach to examine the relationship between Oct-4 and t 12 is to analyze the genomic structure and sequence of ...
... the relevant parental t-haplotypes. This lack of polymorphism was not unexpected because all t-haplotypes are generally believed to be descended from a single ancestral chromosome. Another approach to examine the relationship between Oct-4 and t 12 is to analyze the genomic structure and sequence of ...
Synthesis of Oligonucleotides
... Nucleobases. Permanent protecting groups for the exocyclic amino groups of adenine, cytosine and guanine have been used for many years in oligonucleotide synthesis.1 Acyl protecting groups were chosen, since they are stable for long periods during mildly basic and acidic conditions used during oligo ...
... Nucleobases. Permanent protecting groups for the exocyclic amino groups of adenine, cytosine and guanine have been used for many years in oligonucleotide synthesis.1 Acyl protecting groups were chosen, since they are stable for long periods during mildly basic and acidic conditions used during oligo ...
Document
... thousands of GeneChips are freely available in the public domain, in repositories such as GEO. We are mining this data. We are able to discover signals in GeneChips surveys which will be invisible to analysts dealing with single experiments. We are developing tools to enable analysts of single exper ...
... thousands of GeneChips are freely available in the public domain, in repositories such as GEO. We are mining this data. We are able to discover signals in GeneChips surveys which will be invisible to analysts dealing with single experiments. We are developing tools to enable analysts of single exper ...
Eds., N. Hamamura, S. Suzuki, S. Mendo, C. M. Barroso,... © by TERRAPUB, 2010.
... are shown the DGGE profiles for the samples with (16, 17, 18, 28, 29, 39) and without (31, 32, 33, 43, 44, 45) TBT at times 0 and 30 (Table 4). It is possible to observe that the microbial community is relatively stable along the 30 days. As expected, a higher number of bands appeared, corresponding ...
... are shown the DGGE profiles for the samples with (16, 17, 18, 28, 29, 39) and without (31, 32, 33, 43, 44, 45) TBT at times 0 and 30 (Table 4). It is possible to observe that the microbial community is relatively stable along the 30 days. As expected, a higher number of bands appeared, corresponding ...
Science and the Dead - Council for British Archaeology
... The scientific study of ancient human skeletons has traditionally relied upon measurement and visual examination of the remains. In recent years, scientific techniques involving destructive analysis of samples taken from bones or teeth have become increasingly important. The earliest of these labora ...
... The scientific study of ancient human skeletons has traditionally relied upon measurement and visual examination of the remains. In recent years, scientific techniques involving destructive analysis of samples taken from bones or teeth have become increasingly important. The earliest of these labora ...
Studies on the key amino acid residues responsible for the alkali
... acidic shift of the pH optimum were detected. Three of them were selected and studied (Table 2). The pH profiles of the three mutant xylanases were also compared with that of the wild type (data not shown). All three mutant xylanases had similar pH profiles as that of N71S, and they did not affect t ...
... acidic shift of the pH optimum were detected. Three of them were selected and studied (Table 2). The pH profiles of the three mutant xylanases were also compared with that of the wild type (data not shown). All three mutant xylanases had similar pH profiles as that of N71S, and they did not affect t ...
Full-Text PDF
... Digital microfluidic (DMF) technology provides an alternative method for performing fully programmable automated microfluidic assays using nL volume liquid droplets. DMF exploits the phenomenon of electrowetting-on-dielectric (EWOD) to move liquids [29]. Droplets of liquid can be manipulated in two ...
... Digital microfluidic (DMF) technology provides an alternative method for performing fully programmable automated microfluidic assays using nL volume liquid droplets. DMF exploits the phenomenon of electrowetting-on-dielectric (EWOD) to move liquids [29]. Droplets of liquid can be manipulated in two ...
An rpoB signature sequence provides unique resolution for the
... nucleotide alignments by using three methods: neighbour-joining (NJ), maximum-parsimony (MP) and maximum-likelihood (ML). NJ (Jukes–Cantor model) and MP trees were calculated by using MEGA4 (Tamura et al., 2007). ML trees were computed with Phyml v.2.4.1 (Guindon & Gascuel, 2003) and the HKY (Hasega ...
... nucleotide alignments by using three methods: neighbour-joining (NJ), maximum-parsimony (MP) and maximum-likelihood (ML). NJ (Jukes–Cantor model) and MP trees were calculated by using MEGA4 (Tamura et al., 2007). ML trees were computed with Phyml v.2.4.1 (Guindon & Gascuel, 2003) and the HKY (Hasega ...
Conformational Changes in HIV-1 Reverse Transcriptase Induced
... into the newly synthesized DNA chain [23]. Incorporation of an NRTI into the nascent viral DNA chain results in termination of any further nucleic acid synthesis. NNRTIs are chemically distinct from nucleosides and unlike the NRTIs, they do not require intracellular metabolism for activity. In gener ...
... into the newly synthesized DNA chain [23]. Incorporation of an NRTI into the nascent viral DNA chain results in termination of any further nucleic acid synthesis. NNRTIs are chemically distinct from nucleosides and unlike the NRTIs, they do not require intracellular metabolism for activity. In gener ...
Fulltext PDF - Indian Academy of Sciences
... was found to be reset in the progeny of plants induced to flower early with 5-azacytidine, paralleling the lack of inheritance of the vernalized condition. Based on these findings, Burn et al (1993) proposed that vernalization, through general demethylating effects, may release the block to flowerin ...
... was found to be reset in the progeny of plants induced to flower early with 5-azacytidine, paralleling the lack of inheritance of the vernalized condition. Based on these findings, Burn et al (1993) proposed that vernalization, through general demethylating effects, may release the block to flowerin ...
Mycobacterium kyorinense sp. nov., a novel, slow
... fastness were determined by Ziehl-Neelsen staining as described by Chapin (2007). Colony morphology, pigmentation, and the ability of the isolates to grow at various temperatures (25, 28, 30, 35 and 42 uC) were observed on Middlebrook 7H11-OADC agar (Nippon Becton Dickinson) and 1 % Ogawa egg agar ( ...
... fastness were determined by Ziehl-Neelsen staining as described by Chapin (2007). Colony morphology, pigmentation, and the ability of the isolates to grow at various temperatures (25, 28, 30, 35 and 42 uC) were observed on Middlebrook 7H11-OADC agar (Nippon Becton Dickinson) and 1 % Ogawa egg agar ( ...
Systematic Mutational Analysis of the Yeast ACT1 Gene.
... is far removed from the first two (glutamate 259 to valine [E259V]). These three should represent only a small fraction of the many potential binding and otherfunctional domains of the protein. Recently, powerful general techniques for systematic mutagenesis of protein coding sequenceshave beendevel ...
... is far removed from the first two (glutamate 259 to valine [E259V]). These three should represent only a small fraction of the many potential binding and otherfunctional domains of the protein. Recently, powerful general techniques for systematic mutagenesis of protein coding sequenceshave beendevel ...
Analysis of Cross Sequence Similarities for Multiple - PolyU
... sample sequence while the subsequence ‘ACGCAT’ listed between 7 and 12 is the 7th to 12th bases of the sample sequence. The vertical line located between two bases indicates that the upper base is identical to the lower base. By comparing the first subsequence (1st to 6th bases) and the second subse ...
... sample sequence while the subsequence ‘ACGCAT’ listed between 7 and 12 is the 7th to 12th bases of the sample sequence. The vertical line located between two bases indicates that the upper base is identical to the lower base. By comparing the first subsequence (1st to 6th bases) and the second subse ...
2.2 Australian Pilot Survey for GM Food Labelling
... Four of the five soy milk samples in which GM material was detected had voluntary negative label claims about the GM status of ingredients. The manufacturers of these samples had implemented management systems to determine the GM status of the ingredients used in their food products. The remaining 6 ...
... Four of the five soy milk samples in which GM material was detected had voluntary negative label claims about the GM status of ingredients. The manufacturers of these samples had implemented management systems to determine the GM status of the ingredients used in their food products. The remaining 6 ...
Clinical and Molecular Aspects of Diseases of Mitochondrial DNA
... creation and screening of mutants. Caron et al.(40) first identified a histone-like protein from yeast mitochondria. This protein is encoded by the ABF2 gene. It has two high-mobility-group (HMG) domains capable of binding DNA and is required for maintenance of mtDNA.(41) The bacterial DNA packaging ...
... creation and screening of mutants. Caron et al.(40) first identified a histone-like protein from yeast mitochondria. This protein is encoded by the ABF2 gene. It has two high-mobility-group (HMG) domains capable of binding DNA and is required for maintenance of mtDNA.(41) The bacterial DNA packaging ...
Rosalind Elsie Franklin Physicist www.AssignmentPoint.com
... to by the staff) of Mering on 14 February 1947 as one of the fifteen chercheurs (researchers). ...
... to by the staff) of Mering on 14 February 1947 as one of the fifteen chercheurs (researchers). ...
Identification of Novel Non-Metal Haloperoxidases from the Marine
... However, clones in group B, including CS19 HPO clone, showed relatively higher homology of approximately 80% with 70% identity as shown in Fig. 3. ...
... However, clones in group B, including CS19 HPO clone, showed relatively higher homology of approximately 80% with 70% identity as shown in Fig. 3. ...
Arc Diagrams: Visualizing Structure in Strings
... auto-correlation matrix; in its simplest form, a string of n symbols a1a2…an is represented by an n x n image in which the pixel at coordinates (i,j) is colored black if ai=aj and white otherwise. This image often provides a good picture of the string’s structure, with repeated subsequences showing ...
... auto-correlation matrix; in its simplest form, a string of n symbols a1a2…an is represented by an n x n image in which the pixel at coordinates (i,j) is colored black if ai=aj and white otherwise. This image often provides a good picture of the string’s structure, with repeated subsequences showing ...
the list of 56 genes that the American College of Medical Genetics and Genomics
... here as incidental findings, the analysis may not be technically equivalent to examining these genes as a primary finding. For example, clinical sequencing could have areas of diminished or absent coverage in the genes examined for incidental findings that would be filled in by Sanger sequencing or ...
... here as incidental findings, the analysis may not be technically equivalent to examining these genes as a primary finding. For example, clinical sequencing could have areas of diminished or absent coverage in the genes examined for incidental findings that would be filled in by Sanger sequencing or ...
animal genetics
... Two organisms may appear to be similar, but they can have different genotypes. Similarly, two animals may have the same genotypes, but will appear to be different from each other, if they have been exposed to different environmental conditions throughout their lives. Illustration of DNA Double Heli ...
... Two organisms may appear to be similar, but they can have different genotypes. Similarly, two animals may have the same genotypes, but will appear to be different from each other, if they have been exposed to different environmental conditions throughout their lives. Illustration of DNA Double Heli ...
Power Point
... Two organisms may appear to be similar, but they can have different genotypes. Similarly, two animals may have the same genotypes, but will appear to be different from each other, if they have been exposed to different environmental conditions throughout their lives. Illustration of DNA Double Heli ...
... Two organisms may appear to be similar, but they can have different genotypes. Similarly, two animals may have the same genotypes, but will appear to be different from each other, if they have been exposed to different environmental conditions throughout their lives. Illustration of DNA Double Heli ...
Analysis of Drosophila Species Genome Size and Satellite DNA
... large differences among and within species. Multiple estimates exist for several species and suggest intraspecific genome size differences of up to 50% for some. In strains of D. melanogaster, the intraspecific genome size variation was attributed to differences in heterochromatin content (Halfer 19 ...
... large differences among and within species. Multiple estimates exist for several species and suggest intraspecific genome size differences of up to 50% for some. In strains of D. melanogaster, the intraspecific genome size variation was attributed to differences in heterochromatin content (Halfer 19 ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).