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... if modified bases are considered or if base pairs are formed. Details of the conformation of a nucleotide are accurately defined by a series of torsion angles specific for each bond. Each torsion angle accurately depicts the geometric relationship of four atoms in a sequence, e.g. A–B–C–D. The angle i ...
... if modified bases are considered or if base pairs are formed. Details of the conformation of a nucleotide are accurately defined by a series of torsion angles specific for each bond. Each torsion angle accurately depicts the geometric relationship of four atoms in a sequence, e.g. A–B–C–D. The angle i ...
Press release
... The oKtopure from LGC was developed to speed up breeding programs and many other molecular biological analyses. The robot allows 8 x 96 Deepwell plates to be purified in parallel, enabling up to 5,000 samples to be extracted in the course of a normal eight-hour working day. The precise throughput de ...
... The oKtopure from LGC was developed to speed up breeding programs and many other molecular biological analyses. The robot allows 8 x 96 Deepwell plates to be purified in parallel, enabling up to 5,000 samples to be extracted in the course of a normal eight-hour working day. The precise throughput de ...
Supplementary Materials and methods (doc 154K)
... before and after the competition. The fitness of each strain was calculated using 3 independent competition experiments with 5 technical replicates per experiment. In every assay, wild type PAO1 was competed with PAO1-GFP to set the discriminatory gates for the flow cytometer and to calculate the re ...
... before and after the competition. The fitness of each strain was calculated using 3 independent competition experiments with 5 technical replicates per experiment. In every assay, wild type PAO1 was competed with PAO1-GFP to set the discriminatory gates for the flow cytometer and to calculate the re ...
Characterization of the Arabidopsis thaliana Mutant pcb2 which
... in higher plants (Lange and Ghassemian 2003). Two glutamates form a porphobilinogen after the conversion to 5-aminolevulinic acids, and then four porphobilinogens make up a tetrapyrol ring that leads to protoporphyrin IX after some modifications. Insertion of a Mg ion into protoporphyrin IX by Mg-ch ...
... in higher plants (Lange and Ghassemian 2003). Two glutamates form a porphobilinogen after the conversion to 5-aminolevulinic acids, and then four porphobilinogens make up a tetrapyrol ring that leads to protoporphyrin IX after some modifications. Insertion of a Mg ion into protoporphyrin IX by Mg-ch ...
Microplate-Based Pathlength Correction Method for Photometric
... 0.3–0.5 µg/ml of DNA with a 384-well plate, and between 0.5–0.9 µg/ml with a 96-well plate. This is the lowest concentration that can be reliably separated from the blank level in order to determine whether a sample contains DNA or not. For reliable numeric concentration values one must have at leas ...
... 0.3–0.5 µg/ml of DNA with a 384-well plate, and between 0.5–0.9 µg/ml with a 96-well plate. This is the lowest concentration that can be reliably separated from the blank level in order to determine whether a sample contains DNA or not. For reliable numeric concentration values one must have at leas ...
Discovery of Paralogous Nuclear Gene Sequences Coding for the
... Eighteen land plant RPB2 sequences were selected for relative rate tests according to the method of Steel et al. (1996) as implemented in the computer program r8s (Sanderson 1997). These 18 sequences were selected because they best represented the land plant RPB2 sequences available and because they ...
... Eighteen land plant RPB2 sequences were selected for relative rate tests according to the method of Steel et al. (1996) as implemented in the computer program r8s (Sanderson 1997). These 18 sequences were selected because they best represented the land plant RPB2 sequences available and because they ...
no correlation between cyp17-34t/c polymorphism and severe acne
... Acne vulgaris is the most common skin condition treated by physician. As a multi factors disease of the pilosebaceous apparatus characterized by abnormal desquamation of follicular epithelial cells, increased sebum production, and proliferation of Propionibacterium acnes (which induces inflammation) ...
... Acne vulgaris is the most common skin condition treated by physician. As a multi factors disease of the pilosebaceous apparatus characterized by abnormal desquamation of follicular epithelial cells, increased sebum production, and proliferation of Propionibacterium acnes (which induces inflammation) ...
Genetic Polymorphism of Human CYP2E1
... the CYP2E1*2 and CYP2E1*3 alleles, site-directed mutagenesis was used to introduce these mutations into the wild-type CYP2E1 cDNA. The cDNAs were inserted into the pCMV4 expression vector and subsequently expressed in COS-1 cells. For comparison, cells transfected with the vector alone were used as ...
... the CYP2E1*2 and CYP2E1*3 alleles, site-directed mutagenesis was used to introduce these mutations into the wild-type CYP2E1 cDNA. The cDNAs were inserted into the pCMV4 expression vector and subsequently expressed in COS-1 cells. For comparison, cells transfected with the vector alone were used as ...
Novel pathogen-specific primers for the detection of Agrobacterium
... crown gall (BURR et al. 1998). Besides A. vitis, Agrobacterium tumefaciens may also occur on grapevines as causative agent of crown gall disease (SZEGEDI et al. 2005). The polymerase chain reaction (PCR) has widely been used to test for the presence of various plant pathogens to select clean plant m ...
... crown gall (BURR et al. 1998). Besides A. vitis, Agrobacterium tumefaciens may also occur on grapevines as causative agent of crown gall disease (SZEGEDI et al. 2005). The polymerase chain reaction (PCR) has widely been used to test for the presence of various plant pathogens to select clean plant m ...
Are Restriction Enzymes Recognition Sites Underrepresented in the
... that many restriction enzymes had their recognition sites underrepresented in their host genome. Adaptive bacterial ada regulon system, a system that protects bacterial genome against exogenous DNA methylation by environmental mutagens interacted with the restriction modification system and may have ...
... that many restriction enzymes had their recognition sites underrepresented in their host genome. Adaptive bacterial ada regulon system, a system that protects bacterial genome against exogenous DNA methylation by environmental mutagens interacted with the restriction modification system and may have ...
The Mobile Genetic Element Alu in the Human Genome
... Alu sequences represent the largest family of short interspersed repetitive elements (SINEs) in humans, being present in an excess of 500,000 copies per haploid genome. Alu elements, as well as the other repetitive elements, were once considered to be useless. Today, the biology of Alu transposable ...
... Alu sequences represent the largest family of short interspersed repetitive elements (SINEs) in humans, being present in an excess of 500,000 copies per haploid genome. Alu elements, as well as the other repetitive elements, were once considered to be useless. Today, the biology of Alu transposable ...
Introductory genetics for veterinary students
... Is it reasonable to assume that the Q and q alleles will be alternatively “fixed” in the alternate F0 lines? Hence, that the QTL genotype of all F1 animals is the same? Especially when this is not the case for the SNP or microsatellite markers. This has lead to many erroneous conclusions of impr ...
... Is it reasonable to assume that the Q and q alleles will be alternatively “fixed” in the alternate F0 lines? Hence, that the QTL genotype of all F1 animals is the same? Especially when this is not the case for the SNP or microsatellite markers. This has lead to many erroneous conclusions of impr ...
Transcription
... • There isn’t a single DNA sequence that is used as a promoter. Instead, promoters have a consensus sequence: all promoters are similar to but not necessarily identical to the consensus. • Bacterial promoters consist of 2 regions of about 6 bases, located about 10 and 35 bases upstream from the firs ...
... • There isn’t a single DNA sequence that is used as a promoter. Instead, promoters have a consensus sequence: all promoters are similar to but not necessarily identical to the consensus. • Bacterial promoters consist of 2 regions of about 6 bases, located about 10 and 35 bases upstream from the firs ...
Repair of Site-Specific DNA Double-Strand Breaks in
... a double Holliday junction is formed that can be resolved as noncrossover or as crossover (representing an exchange of flanking sequences). Depending on the template involved in Holliday junctions, the crossover-type resolution can result in a sister chromatid exchange (SCE), a crossing over between ...
... a double Holliday junction is formed that can be resolved as noncrossover or as crossover (representing an exchange of flanking sequences). Depending on the template involved in Holliday junctions, the crossover-type resolution can result in a sister chromatid exchange (SCE), a crossing over between ...
Mycobacterium tuberculosis DNA gyrase ATPase domain structures
... problems of DNA by temporarily introducing a double-stranded break into one DNA segment and transporting another DNA [TDNA (transported DNA)] duplex through this temporary break [1,2]. Most bacteria have two type II topoisomerases (DNA gyrase and Topo IV), which are are heterotetrameric enzymes comp ...
... problems of DNA by temporarily introducing a double-stranded break into one DNA segment and transporting another DNA [TDNA (transported DNA)] duplex through this temporary break [1,2]. Most bacteria have two type II topoisomerases (DNA gyrase and Topo IV), which are are heterotetrameric enzymes comp ...
The msh2 Gene of Schizosaccharomyces pombe Is
... TTGG-39). For both reactions, the program consisted of an initial 5-min denaturation at 94°C, 30 cycles of 45 s 94°C, 45 s 48°C, and 2.5 min 72°C, and finally an extension step of 10 min at 72°C. The PCR products were separated from primers, and the single coding strands were amplified by 20 cycles ...
... TTGG-39). For both reactions, the program consisted of an initial 5-min denaturation at 94°C, 30 cycles of 45 s 94°C, 45 s 48°C, and 2.5 min 72°C, and finally an extension step of 10 min at 72°C. The PCR products were separated from primers, and the single coding strands were amplified by 20 cycles ...
A large scale analysis of resistance gene
... work in many labs is clarifying the structure and mode of action of the resistance genes (R-genes) involved in this type of response. A number of R-genes that control disease resistance of this type have been identified in the model plant A. thaliana (eg. Simonich and Innes 1995; Gassmann et al. 1999 ...
... work in many labs is clarifying the structure and mode of action of the resistance genes (R-genes) involved in this type of response. A number of R-genes that control disease resistance of this type have been identified in the model plant A. thaliana (eg. Simonich and Innes 1995; Gassmann et al. 1999 ...
MIDWESTERN ASSOCIATION OF FORENSIC SCIENTISTS, INC
... opportunity to examine a variety of dusts, including those which they themselves bring with them. The manner in which a dust sample has been collected has an important bearing on the techniques used to examine it. Dust may be received as a loose powder, from vacuuming, as scrapings, adhering to adhe ...
... opportunity to examine a variety of dusts, including those which they themselves bring with them. The manner in which a dust sample has been collected has an important bearing on the techniques used to examine it. Dust may be received as a loose powder, from vacuuming, as scrapings, adhering to adhe ...
Defining characteristics of Tn5 Transposase non
... proteolytic fragments of MuA reveal that the N-terminal 26 amino acids of domain III binds supercoiled DNA nonspecifically (3) and that domain II can interact with linear non-specific DNA (4). The Tn3 Tnp binds non-specific linear restriction fragments (5,6). Finally, many Tnps, including Tn5 Tnp, c ...
... proteolytic fragments of MuA reveal that the N-terminal 26 amino acids of domain III binds supercoiled DNA nonspecifically (3) and that domain II can interact with linear non-specific DNA (4). The Tn3 Tnp binds non-specific linear restriction fragments (5,6). Finally, many Tnps, including Tn5 Tnp, c ...
pyrimidine
... Incorporates DNA replication as an analytical tool for determining sequence Uses short primer that attaches to the 3’ end of the ssDNA, after which a specially engineered DNA polymerase Each vial includes one dideoxyXTP and 3 ordinary dXTPs; the dideoxyXTP will be incorporated but will halt synthesi ...
... Incorporates DNA replication as an analytical tool for determining sequence Uses short primer that attaches to the 3’ end of the ssDNA, after which a specially engineered DNA polymerase Each vial includes one dideoxyXTP and 3 ordinary dXTPs; the dideoxyXTP will be incorporated but will halt synthesi ...
Chpt8_RecombineDNA.doc
... RECOMBINATION OF DNA The previous chapter on mutation and repair of DNA dealt mainly with small changes in DNA sequence, usually single base pairs, resulting from errors in replication or damage to DNA. The DNA sequence of a chromosome can change in large segments as well, by the processes of recomb ...
... RECOMBINATION OF DNA The previous chapter on mutation and repair of DNA dealt mainly with small changes in DNA sequence, usually single base pairs, resulting from errors in replication or damage to DNA. The DNA sequence of a chromosome can change in large segments as well, by the processes of recomb ...
DNA Three Way Junction Core Decorated with Amino Acids
... The elucidation of the peptide bond formation within ribosome showed that the reaction was catalyzed by ribonucleic acids moieties without any participation of protein amino acids residues in the active site [1,2]. Therefore, it has been tempting to search for nucleic acid catalysts that could perfo ...
... The elucidation of the peptide bond formation within ribosome showed that the reaction was catalyzed by ribonucleic acids moieties without any participation of protein amino acids residues in the active site [1,2]. Therefore, it has been tempting to search for nucleic acid catalysts that could perfo ...
pdf
... RECOMBINATION OF DNA The previous chapter on mutation and repair of DNA dealt mainly with small changes in DNA sequence, usually single base pairs, resulting from errors in replication or damage to DNA. The DNA sequence of a chromosome can change in large segments as well, by the processes of recomb ...
... RECOMBINATION OF DNA The previous chapter on mutation and repair of DNA dealt mainly with small changes in DNA sequence, usually single base pairs, resulting from errors in replication or damage to DNA. The DNA sequence of a chromosome can change in large segments as well, by the processes of recomb ...
(base) sequence of the genome might reflect biological information
... sequences of an individual gene should vary in the genome because the protein- encoding gene and the adjoining (5'- and 3'-) non-coding base sequences were different. In other words, the appearance frequencies of the base sequences should be different for each gene. Even if the base sequences of the ...
... sequences of an individual gene should vary in the genome because the protein- encoding gene and the adjoining (5'- and 3'-) non-coding base sequences were different. In other words, the appearance frequencies of the base sequences should be different for each gene. Even if the base sequences of the ...
2. Assessing the probative value of DNA evidence
... Building on this general introduction, Guide No 2 explores in the following pages the probabilistic foundations of DNA profiling evidence and considers how to evaluate its probative value in criminal trials. The remaining two Guides will give detailed consideration to: (3) networks for structuring e ...
... Building on this general introduction, Guide No 2 explores in the following pages the probabilistic foundations of DNA profiling evidence and considers how to evaluate its probative value in criminal trials. The remaining two Guides will give detailed consideration to: (3) networks for structuring e ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).