Prediction and investigation of novel proteins in DNA double
... DNA double stranded breaks (DSBs) are the most genotoxic forms of DNA lesions, causing fragmentation of the DNA strands. Mis-repaired and unrepaired DSBs lead to chromosomal rearrangement and genomic instability promoting tumorigenesis or cell death. DSBs are primarily repaired by two independent an ...
... DNA double stranded breaks (DSBs) are the most genotoxic forms of DNA lesions, causing fragmentation of the DNA strands. Mis-repaired and unrepaired DSBs lead to chromosomal rearrangement and genomic instability promoting tumorigenesis or cell death. DSBs are primarily repaired by two independent an ...
Identification and isolation of active N2O reducers in rice paddy soil
... Evaluation of the soil microcosm. Based on the preliminary experiments, all of the added N2O disappeared within 24 h of incubation when <2% N2O was added (data not shown). Since N2O should always be present to minimize utilization of succinate by metal reducers, the concentration of N2O should be >2 ...
... Evaluation of the soil microcosm. Based on the preliminary experiments, all of the added N2O disappeared within 24 h of incubation when <2% N2O was added (data not shown). Since N2O should always be present to minimize utilization of succinate by metal reducers, the concentration of N2O should be >2 ...
Multiple domain structure in a chitinase gene (chic)
... polyvinylidene difluoride transfer membrane (Immobilon-P, Millipore). The bands corresponding to chitinase C and D were cut from the membrane and subjected to N-terminal amino acid sequence analysis using an automated protein sequencer (model 477A, Applied Biosystems). ...
... polyvinylidene difluoride transfer membrane (Immobilon-P, Millipore). The bands corresponding to chitinase C and D were cut from the membrane and subjected to N-terminal amino acid sequence analysis using an automated protein sequencer (model 477A, Applied Biosystems). ...
Statistical analysis of simple repeats in the human genome
... associated with any of the single- or di-nucleotide probabilities, nor with any mnucleotide probability, with m42: This finding is consistent with the general acceptance of the failure of zeroth- and first-order Markovian models of simple repeats to fully account for ‘‘linguistic’’ features of non-cod ...
... associated with any of the single- or di-nucleotide probabilities, nor with any mnucleotide probability, with m42: This finding is consistent with the general acceptance of the failure of zeroth- and first-order Markovian models of simple repeats to fully account for ‘‘linguistic’’ features of non-cod ...
A Pneumocystis carinii multi-gene family with
... contained a poly(A) tail. One recombinant, 73j, was selected for further analysis and the recombinant DNA was sequenced in full from both strands. DNA amplification. Oligonucleotide primers were designed to hybridize to various regions of the P. carinii PRTI nucleotide sequences (Fig. 1, Table 1). S ...
... contained a poly(A) tail. One recombinant, 73j, was selected for further analysis and the recombinant DNA was sequenced in full from both strands. DNA amplification. Oligonucleotide primers were designed to hybridize to various regions of the P. carinii PRTI nucleotide sequences (Fig. 1, Table 1). S ...
Genomic analysis of clinical samples with serologic ABO blood
... subgroups. Each allele was characterized by a missense or nonsense mutation for which screening by allele-specific primer polymerase chain reaction was performed. The novel mutations were encountered in 28 of the remaining 60 A and B subgroup samples but not among normal donors. As a result of this ...
... subgroups. Each allele was characterized by a missense or nonsense mutation for which screening by allele-specific primer polymerase chain reaction was performed. The novel mutations were encountered in 28 of the remaining 60 A and B subgroup samples but not among normal donors. As a result of this ...
Molecular Biology and Applied Genetics
... molecular background to enable them to understand and critically analyze recent advances in laboratory sciences. Finally, it contains a glossary, which summarizes important terminologies used in the text. Each chapter begins by specific learning objectives and at the end of each chapter review quest ...
... molecular background to enable them to understand and critically analyze recent advances in laboratory sciences. Finally, it contains a glossary, which summarizes important terminologies used in the text. Each chapter begins by specific learning objectives and at the end of each chapter review quest ...
Structural Energetics of a RNA-DNA Hybrid
... form a complementary RNA strand, and protein is synthesized from this geneencoded RNA strand. A diagram of this process is shown in Figure 1.1.4. In this study, we are focused on the transcription step in the transfer of genetic information. The formation of the RNA transcript is divided into two ph ...
... form a complementary RNA strand, and protein is synthesized from this geneencoded RNA strand. A diagram of this process is shown in Figure 1.1.4. In this study, we are focused on the transcription step in the transfer of genetic information. The formation of the RNA transcript is divided into two ph ...
FEMS Microbiology Letters
... In the pyruvate clastic reaction [1], PFO catalyses the oxidative cleavage of pyruvate and coenzyme A to acetylcoenzyme A and CO2 with concomittant reduction of ferredoxin. The enzyme has been described for strictly anaerobic bacteria like Clostridium spp. [1], diverse archaea [2] and some eukaryote ...
... In the pyruvate clastic reaction [1], PFO catalyses the oxidative cleavage of pyruvate and coenzyme A to acetylcoenzyme A and CO2 with concomittant reduction of ferredoxin. The enzyme has been described for strictly anaerobic bacteria like Clostridium spp. [1], diverse archaea [2] and some eukaryote ...
A homozygous double mutation in SMN1
... exon 1 and exon 5 mutations and one normal allele. Prenatal testing for mutation detection was requested several months later when this mother became pregnant. However, the DNA sequences of affected individuals are indistinguishable from one-copy carriers of the mutated allele because of the SMN2 ba ...
... exon 1 and exon 5 mutations and one normal allele. Prenatal testing for mutation detection was requested several months later when this mother became pregnant. However, the DNA sequences of affected individuals are indistinguishable from one-copy carriers of the mutated allele because of the SMN2 ba ...
Investigating the DNA-Binding Interactions of Small
... Theodore Goodson III for the opportunity. He is one of the few people who has stood up and fought for me. Without him, this would have not been possible. He has provided me the guidance, support, and resources to be successful as a scientist. I will be forever thankful for Prof. Goodson. He will be ...
... Theodore Goodson III for the opportunity. He is one of the few people who has stood up and fought for me. Without him, this would have not been possible. He has provided me the guidance, support, and resources to be successful as a scientist. I will be forever thankful for Prof. Goodson. He will be ...
Similarity Searches on Sequence Databases: BLAST
... Choosing the right Parameters: sequence masking • When BLAST searches databases, it makes the assumption that the average composition of any sequence is the same as the average composition of the whole database. • However this assumption doesn’t hold all the time, some sequences have biased composi ...
... Choosing the right Parameters: sequence masking • When BLAST searches databases, it makes the assumption that the average composition of any sequence is the same as the average composition of the whole database. • However this assumption doesn’t hold all the time, some sequences have biased composi ...
NUCLEIC ACID ECONOMY IN BACTERIA INFECTED WITH
... infection. The sink contains non-precursor phosphorus. Unidentified DNA can be determined in two ways. First, one can assay cytosineless DNA chromatographically, and subtract from this result the mature phage DNA (Hershey, Dixon, and Chase, 1953). Second, one can measure the DNA-P synthesized after ...
... infection. The sink contains non-precursor phosphorus. Unidentified DNA can be determined in two ways. First, one can assay cytosineless DNA chromatographically, and subtract from this result the mature phage DNA (Hershey, Dixon, and Chase, 1953). Second, one can measure the DNA-P synthesized after ...
Systematic and Applied Microbiology - digital
... of the gene coding for this protein in the E. faecium strains under study, two oligonucleotides were designed. We used primer 57 (5´-ATGAGTGAATCATTGTCG3´) and 58 (5´-TTATTTTGCTTCGCTTGCC-3´) to amplify a 1.9 kb DNA fragment including the complete gene coding for the putative tyrosine decarboxylase. P ...
... of the gene coding for this protein in the E. faecium strains under study, two oligonucleotides were designed. We used primer 57 (5´-ATGAGTGAATCATTGTCG3´) and 58 (5´-TTATTTTGCTTCGCTTGCC-3´) to amplify a 1.9 kb DNA fragment including the complete gene coding for the putative tyrosine decarboxylase. P ...
Apolipoprotein E Allele Distribution in Trisomy
... higher frequency of apoE e4 alleles in patients with Alzheimer disease, while in patients with Down syndrome, the frequency did not differ from that of the healthy control group. The mechanism of the deposition of senile plaques seems to be different. In trisomy 21, there are large plaques reflectin ...
... higher frequency of apoE e4 alleles in patients with Alzheimer disease, while in patients with Down syndrome, the frequency did not differ from that of the healthy control group. The mechanism of the deposition of senile plaques seems to be different. In trisomy 21, there are large plaques reflectin ...
Pourcel et al., Microbiology 2005
... CRISPR polymorphism in Y. pestis In all three sequenced genomes, three CRISPR elements are found. One of them, called here YP1, is associated with the cas1 and cas3 genes and is located in the vicinity of the replication terminus (Fig. 1). The relative position of YP1, YP2 and YP3 varies in the thre ...
... CRISPR polymorphism in Y. pestis In all three sequenced genomes, three CRISPR elements are found. One of them, called here YP1, is associated with the cas1 and cas3 genes and is located in the vicinity of the replication terminus (Fig. 1). The relative position of YP1, YP2 and YP3 varies in the thre ...
CH Zinc Fingers As DNA Binding Domains
... Data are taken from Pfam 9.0 a t Washington Uninversity i n St. Louis. *The number is f o r proteins containing two C2C2 fingers. finger proteins can be divided into four classes (Fig. 2), (A) single C2H2, (B) triple C2H2, (C) multiple-adjacent C2H2, and (D) separated-paired C2H2 zinc finger protein ...
... Data are taken from Pfam 9.0 a t Washington Uninversity i n St. Louis. *The number is f o r proteins containing two C2C2 fingers. finger proteins can be divided into four classes (Fig. 2), (A) single C2H2, (B) triple C2H2, (C) multiple-adjacent C2H2, and (D) separated-paired C2H2 zinc finger protein ...
(Pan) Salmonella and Salmonella Serotype Typhi–Specific Real
... (ie, lactose non-fermenting colonies with or without hydrogen sulfide production) are submitted for additional screening or identification, depending on individual laboratory practices.5-7 These practices, although effective, are timeconsuming and costly. The identification of Salmonella directly fr ...
... (ie, lactose non-fermenting colonies with or without hydrogen sulfide production) are submitted for additional screening or identification, depending on individual laboratory practices.5-7 These practices, although effective, are timeconsuming and costly. The identification of Salmonella directly fr ...
Characterization of the chimeric seven
... Recently, many PR-like genes were found in non-marine environments. The goal of this study is to explore the function of rhodopsins that exist only as partial proteoopsin genes using chimeras with marine green PR (GPR). We isolated nine partial genes of PR homologues using polymerase chain reaction ...
... Recently, many PR-like genes were found in non-marine environments. The goal of this study is to explore the function of rhodopsins that exist only as partial proteoopsin genes using chimeras with marine green PR (GPR). We isolated nine partial genes of PR homologues using polymerase chain reaction ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).