A High Density Integrated Genetic Linkage Map of Soybean and the
... be the most abundant source of DNA polymorphisms in soybean (Hyten et al., 2006; Zhu et al., 2003). Despite being the most common molecular marker in soybean, the SNP frequency is relatively low compared to other cultivated crop species (Hyten et al., 2006; Zhu et al., 2003). The relatively low sequ ...
... be the most abundant source of DNA polymorphisms in soybean (Hyten et al., 2006; Zhu et al., 2003). Despite being the most common molecular marker in soybean, the SNP frequency is relatively low compared to other cultivated crop species (Hyten et al., 2006; Zhu et al., 2003). The relatively low sequ ...
Document
... Mendel’s Law of Segregation A. Law of Segregation states that a pair of factors (alleles) is segregated, or separated, during the formation of gametes (reproductive cells) (1) When two gametes combine during fertilization, the offspring have two factors controlling a specific trait (Gg) ...
... Mendel’s Law of Segregation A. Law of Segregation states that a pair of factors (alleles) is segregated, or separated, during the formation of gametes (reproductive cells) (1) When two gametes combine during fertilization, the offspring have two factors controlling a specific trait (Gg) ...
Resolving Individuals Contributing Trace Amounts of DNA to Highly
... community [8,15]. However, most SNP assays are inherently quantitative at one or both alleles, requiring a genotype calling algorithm to digitize the inherently analog information of a SNP assay [16]. Within this paper, we specifically exploit raw allele intensity measures for analysis of DNA with m ...
... community [8,15]. However, most SNP assays are inherently quantitative at one or both alleles, requiring a genotype calling algorithm to digitize the inherently analog information of a SNP assay [16]. Within this paper, we specifically exploit raw allele intensity measures for analysis of DNA with m ...
Characterisation of Aeromonas species isolated from diseased fish
... 18 strains of Aeromonas were isolated from healthy or diseased fish and water samples collected from several areas of the Vidharbha region of Maharashtra state, India, for identification and characterization. Aeromonas isolates were characterized by Enterobacterial Repetitive Intergenic Consensus (E ...
... 18 strains of Aeromonas were isolated from healthy or diseased fish and water samples collected from several areas of the Vidharbha region of Maharashtra state, India, for identification and characterization. Aeromonas isolates were characterized by Enterobacterial Repetitive Intergenic Consensus (E ...
DNA Evolution 3.1 Troubleshooting and Debugging Guide
... Since the system logging system is not available on Windows. You can enable logging using the dbgview.exe application. This is a Windows debugging tool that's available from the following link: http://download.sysinternals.com/files/DebugView.zip You can simple download the application and run it. N ...
... Since the system logging system is not available on Windows. You can enable logging using the dbgview.exe application. This is a Windows debugging tool that's available from the following link: http://download.sysinternals.com/files/DebugView.zip You can simple download the application and run it. N ...
Characterization of the unique intron
... Sequencing, Southern and Northern blots The nucleotide sequence of all clones was determined and analyzed as described (4). Southern and Northern blot analysis were performed as described previously (4). ...
... Sequencing, Southern and Northern blots The nucleotide sequence of all clones was determined and analyzed as described (4). Southern and Northern blot analysis were performed as described previously (4). ...
Evolution of a pseudo-control region in the mitochondrial genome of
... detect differentiation at low taxonomic levels. Since our study deals with low level systematics, it seemed advisable to use the most variable part of the mitochondrial (mt) genome, the control region (CR), as a phylogenetic marker. Sequencing of the first complete vertebrate mt genomes suggested th ...
... detect differentiation at low taxonomic levels. Since our study deals with low level systematics, it seemed advisable to use the most variable part of the mitochondrial (mt) genome, the control region (CR), as a phylogenetic marker. Sequencing of the first complete vertebrate mt genomes suggested th ...
Phytochemical and genetic analyses of ancient
... (Taberlet et al., 1991). The reaction mixtures were subjected firstly to an initial heat denaturation at 94 C for 3 min; then, they were subjected to 35 cycles of heat denaturation at 94 C for 30 s, 1 min of primer annealing at 55 C for the ITS region, and 50 C for cpDNA, and DNA extension at 72 ...
... (Taberlet et al., 1991). The reaction mixtures were subjected firstly to an initial heat denaturation at 94 C for 3 min; then, they were subjected to 35 cycles of heat denaturation at 94 C for 30 s, 1 min of primer annealing at 55 C for the ITS region, and 50 C for cpDNA, and DNA extension at 72 ...
Molecular insights into mitochondrial transcription and its
... mitochondrion contains its own genome, a small circular DNA molecule (mtDNA), encoding essential subunits of the oxidative phosphorylation system. Initiation of mitochondrial transcription involves three proteins, the mitochondrial RNA polymerase, POLRMT, and its two transcription factors, TFAM and ...
... mitochondrion contains its own genome, a small circular DNA molecule (mtDNA), encoding essential subunits of the oxidative phosphorylation system. Initiation of mitochondrial transcription involves three proteins, the mitochondrial RNA polymerase, POLRMT, and its two transcription factors, TFAM and ...
BMC Microbiology - ORBi
... bile salts) and almost 2 logarithms (in presence of 0.5% bile salts). The genotypic analyses using total DNA-DNA hybridization, GC% content, 16S rRNA gene sequencing and multilocus sequencing analysis (MLSA) confirmed the classification of Bifidobacterium. crudilactis and B. mongoliense into two dif ...
... bile salts) and almost 2 logarithms (in presence of 0.5% bile salts). The genotypic analyses using total DNA-DNA hybridization, GC% content, 16S rRNA gene sequencing and multilocus sequencing analysis (MLSA) confirmed the classification of Bifidobacterium. crudilactis and B. mongoliense into two dif ...
Nanopore Unzipping of Individual DNA Hairpin Molecules
... not entail the physical coupling of the molecules under test to a force transducer, very high throughput can be achieved. We used our method to study DNA unzipping kinetics at small forces, which have not been accessed before. We find that in this regime the static unzipping times decrease exponentia ...
... not entail the physical coupling of the molecules under test to a force transducer, very high throughput can be achieved. We used our method to study DNA unzipping kinetics at small forces, which have not been accessed before. We find that in this regime the static unzipping times decrease exponentia ...
Isolation, Characterization, and Annotation: The Search for Novel
... issue worldwide, phage therapy has been once again proposed as an alternate option for use against resistant bacteria. In fact, phages have been reported to be more effective in the treatment of certain infections in humans including Staphylococcus aureus10. The first step to effective practice of p ...
... issue worldwide, phage therapy has been once again proposed as an alternate option for use against resistant bacteria. In fact, phages have been reported to be more effective in the treatment of certain infections in humans including Staphylococcus aureus10. The first step to effective practice of p ...
exon junctions of Euglena gene(s) - DigitalCommons@University of
... (Fig. 1B). LH34 was subcloned as a blunt-ended insert, LH138 as an EcoRP-Sail insert, LH64 as a blunt end-Sall insert, and both CLH22 and CLHO9 as EcoRI/HindIl-blunt ended inserts into the polylinker of pBluescript II KS. Sequencing, Southern and Northern blots The nucleotide sequence of all clones ...
... (Fig. 1B). LH34 was subcloned as a blunt-ended insert, LH138 as an EcoRP-Sail insert, LH64 as a blunt end-Sall insert, and both CLH22 and CLHO9 as EcoRI/HindIl-blunt ended inserts into the polylinker of pBluescript II KS. Sequencing, Southern and Northern blots The nucleotide sequence of all clones ...
Phytopathology
... potato tissue. Additional keywords: blackleg, brown rot, macroarray, microarray, reverse dot blot, ring rot, soft rot. ...
... potato tissue. Additional keywords: blackleg, brown rot, macroarray, microarray, reverse dot blot, ring rot, soft rot. ...
MA112 Expresso® Rhamnose SUMO Cloning and
... is designed for expression in E. coli, fusion clones constructed using this system can also be transferred into eukaryotic expression vectors for expression of SUMO-tagged proteins in mammalian or insect cells. The rhaP BAD promoter is a versatile tool for protein expression. In the absence of rhamn ...
... is designed for expression in E. coli, fusion clones constructed using this system can also be transferred into eukaryotic expression vectors for expression of SUMO-tagged proteins in mammalian or insect cells. The rhaP BAD promoter is a versatile tool for protein expression. In the absence of rhamn ...
transposon
... Transposons that mobilize via DNA are found in both prokaryotes and eukaryotes. A genome may contain both functional and nonfunctional (defective) elements. Often the majority of elements in a eukaryotic genome are defective. A eukaryotic genome contains a large number and variety of transposons. ...
... Transposons that mobilize via DNA are found in both prokaryotes and eukaryotes. A genome may contain both functional and nonfunctional (defective) elements. Often the majority of elements in a eukaryotic genome are defective. A eukaryotic genome contains a large number and variety of transposons. ...
Transcription
... in DNA sequencing. It is used to identify the specific DNA sequences that are bound by a particular protein. ...
... in DNA sequencing. It is used to identify the specific DNA sequences that are bound by a particular protein. ...
RT2 Profiler PCR Arrays: Pathway-focused gene
... resources. Currently, RT2 Profiler PCR Arrays are available for many pathways including apoptosis, inflammation, signal transduction, cancer, and other diseases. Visit the SABiosciences web site (www.SABiosciences.com/ArrayList.php) for a complete list. Table 1. Examples of cataloged pathway-focused ...
... resources. Currently, RT2 Profiler PCR Arrays are available for many pathways including apoptosis, inflammation, signal transduction, cancer, and other diseases. Visit the SABiosciences web site (www.SABiosciences.com/ArrayList.php) for a complete list. Table 1. Examples of cataloged pathway-focused ...
Cloning and sequence analysis of cnaA gene encoding the catalytic
... calcineurin A from ¢lamentous fungi suggested its requirement for hyphal growth and cell cycle regulation [5,6], a putative role of this protein phosphatase in sporulation, salt stress response and the alkaline pH-mediated signal transduction pathway in fungi has not been examined. Interestingly, a ...
... calcineurin A from ¢lamentous fungi suggested its requirement for hyphal growth and cell cycle regulation [5,6], a putative role of this protein phosphatase in sporulation, salt stress response and the alkaline pH-mediated signal transduction pathway in fungi has not been examined. Interestingly, a ...
Characterisation of marsupial PHLDA2 reveals eutherian specific acquisition of imprinting Open Access
... Background: Genomic imprinting causes parent-of-origin specific gene expression by differential epigenetic modifications between two parental genomes. We previously reported that there is no evidence of genomic imprinting of CDKN1C in the KCNQ1 domain in the placenta of an Australian marsupial, the ...
... Background: Genomic imprinting causes parent-of-origin specific gene expression by differential epigenetic modifications between two parental genomes. We previously reported that there is no evidence of genomic imprinting of CDKN1C in the KCNQ1 domain in the placenta of an Australian marsupial, the ...
Get - Wiley Online Library
... if modified bases are considered or if base pairs are formed. Details of the conformation of a nucleotide are accurately defined by a series of torsion angles specific for each bond. Each torsion angle accurately depicts the geometric relationship of four atoms in a sequence, e.g. A–B–C–D. The angle i ...
... if modified bases are considered or if base pairs are formed. Details of the conformation of a nucleotide are accurately defined by a series of torsion angles specific for each bond. Each torsion angle accurately depicts the geometric relationship of four atoms in a sequence, e.g. A–B–C–D. The angle i ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).