Molecular Characterization of a Hamster Oviduct
... of the mature form of the HOGP region. The amino acid sequence of HOGP appeared to have eight potential N-glycosylation sites. Northern blot analysis revealed that a single message of approximately 2.5 kb was present inoviductal RNA but not inthe RNA of several other hamster tissues. The HOGP showed ...
... of the mature form of the HOGP region. The amino acid sequence of HOGP appeared to have eight potential N-glycosylation sites. Northern blot analysis revealed that a single message of approximately 2.5 kb was present inoviductal RNA but not inthe RNA of several other hamster tissues. The HOGP showed ...
Assessing the Probative Value of DNA Evidence
... Building on this general introduction, Guide No 2 explores in the following pages the probabilistic foundations of DNA profiling evidence and considers how to evaluate its probative value in criminal trials. The remaining two Guides will give detailed consideration to: (3) networks for structuring e ...
... Building on this general introduction, Guide No 2 explores in the following pages the probabilistic foundations of DNA profiling evidence and considers how to evaluate its probative value in criminal trials. The remaining two Guides will give detailed consideration to: (3) networks for structuring e ...
Locked Nucleic Acid (LNA™)
... the primer (Figure 5). Another strategy for LNA™ primer design involves incorporating the LNA™ nucleoside one position away from the 3’-end. Di Guisto and colleagues have found that such primers perform better in proof-reading allele-specific extension (PRASE) PCR experiments. (Di Giusto et al. Nucl ...
... the primer (Figure 5). Another strategy for LNA™ primer design involves incorporating the LNA™ nucleoside one position away from the 3’-end. Di Guisto and colleagues have found that such primers perform better in proof-reading allele-specific extension (PRASE) PCR experiments. (Di Giusto et al. Nucl ...
Localization and structural analysis of the ribosomal RNA operons of
... components. Concurrent with these studies, physical mapping of R. sphaeroides genomic DNA indicated that the arrangement of the R. sphaeroides genome was complex, comprised of a large (containing one rm operon) and small (with two rm operons) chromosome as well as several endogenous plasmids (9). Th ...
... components. Concurrent with these studies, physical mapping of R. sphaeroides genomic DNA indicated that the arrangement of the R. sphaeroides genome was complex, comprised of a large (containing one rm operon) and small (with two rm operons) chromosome as well as several endogenous plasmids (9). Th ...
TaqMan-Based Real-Time PCR for Genotyping Common
... corresponding reports of negative associations with disease (2 ). Thus, development of a simple, highthroughput HP genotyping method is needed to facilitate these association studies. Several methods for phenotyping HP1 and HP2 have been described. High-pressure gel-permeation chromatography and ge ...
... corresponding reports of negative associations with disease (2 ). Thus, development of a simple, highthroughput HP genotyping method is needed to facilitate these association studies. Several methods for phenotyping HP1 and HP2 have been described. High-pressure gel-permeation chromatography and ge ...
Classification of Centers for Disease Control Group Eugonic
... The designation Centers for Disease Control Group Eugonic Fermenter 4 (EF-4) was first used by Tatum et al. (1974) for a group of Gram-negative saccharolytic organisms predominantly recovered from human wounds resulting from dog or cat bites. EF-4 isolates are considered to be commensal organisms fo ...
... The designation Centers for Disease Control Group Eugonic Fermenter 4 (EF-4) was first used by Tatum et al. (1974) for a group of Gram-negative saccharolytic organisms predominantly recovered from human wounds resulting from dog or cat bites. EF-4 isolates are considered to be commensal organisms fo ...
Horizontal transfer of non-LTR retrotransposons: artifact or rare event
... The most suitable eukaryotic group for such investigations proved to be Fungi. More than 100 fungal genomes are available for analysis in various genomic databases and the number of projects is constantly increasing (e.g. Galagan et al., 2005). Fungi have small genomes, usually with limited amounts ...
... The most suitable eukaryotic group for such investigations proved to be Fungi. More than 100 fungal genomes are available for analysis in various genomic databases and the number of projects is constantly increasing (e.g. Galagan et al., 2005). Fungi have small genomes, usually with limited amounts ...
Alu Human Polymorphism
... inherited from each parent – Most occurred millions of years ago and are often on both pairs of chromosomes – There are Alu elements that have occurred since humans branched from other primates – This gives rise to dimorphic Alus from the last hundreds of thousands of years – Estimated to jump 1:200 ...
... inherited from each parent – Most occurred millions of years ago and are often on both pairs of chromosomes – There are Alu elements that have occurred since humans branched from other primates – This gives rise to dimorphic Alus from the last hundreds of thousands of years – Estimated to jump 1:200 ...
Epigenetic Control of Apomixis
... small inhibitory RNA (siRNA) and DNA methylation are involved in regulation of gene expression at transcriptional and posttranscriptional levels. Epigenetics refers to the heritable variations in phenotypic/gene expression pattern resulting from the modification of DNA, histones and small-RNA biogen ...
... small inhibitory RNA (siRNA) and DNA methylation are involved in regulation of gene expression at transcriptional and posttranscriptional levels. Epigenetics refers to the heritable variations in phenotypic/gene expression pattern resulting from the modification of DNA, histones and small-RNA biogen ...
Genetic characterization of diplodia resistant in somatic hybrids Citrus unshiu (RGA)
... Citrus is one important crop in agroindustrial. Good quality is one important thing that required citrus to be competitive crop in agroindustrial. Research Institute of Citrus and Subtropic Fruit had efforts to increase quality of citrus in Indonesia. Protoplast fusion of Citrus unshiu and Citrus no ...
... Citrus is one important crop in agroindustrial. Good quality is one important thing that required citrus to be competitive crop in agroindustrial. Research Institute of Citrus and Subtropic Fruit had efforts to increase quality of citrus in Indonesia. Protoplast fusion of Citrus unshiu and Citrus no ...
Plant Functional Genomics Plant Functional Genomics
... 1. Set up 4–6 restriction digestions, each digesting 5 µg pCUGIBAC1 plasmid DNA (with HindIII, EcoRI, or BamHI depending on which enzyme is selected for BAC library construction) in 150 µL 1× TA buffer at 37°C for 2 h. Check 1 µL on a 1% agarose minigel to determine if the plasmid is digested. 2. He ...
... 1. Set up 4–6 restriction digestions, each digesting 5 µg pCUGIBAC1 plasmid DNA (with HindIII, EcoRI, or BamHI depending on which enzyme is selected for BAC library construction) in 150 µL 1× TA buffer at 37°C for 2 h. Check 1 µL on a 1% agarose minigel to determine if the plasmid is digested. 2. He ...
OLSON LAB PROTOCOL: Agarose Gel Electrophoresis using GelRed
... • Gel electrophoresis is a method used to size fractionate, visualise and document DNA samples, such as those resulting from PCR • As DNA molecules are negatively charged, a current can be applied to a gel to draw the fragments toward the cathode (positive pole) • Larger fragments move more slowly t ...
... • Gel electrophoresis is a method used to size fractionate, visualise and document DNA samples, such as those resulting from PCR • As DNA molecules are negatively charged, a current can be applied to a gel to draw the fragments toward the cathode (positive pole) • Larger fragments move more slowly t ...
Genetic dissection of Helicobacter pylori AddAB role in homologous
... at ds ends. The complex, either RecBCD or AddAB, binds a dsDNA end and initiates unwinding and degradation of both strands of DNA (Chedin & Kowalczykowski, 2002). Upon interaction with the host-specific sequence w (8 nt in E. coli and 5 nt in B. subtilis), the mediator complex generates a 3 0 -end s ...
... at ds ends. The complex, either RecBCD or AddAB, binds a dsDNA end and initiates unwinding and degradation of both strands of DNA (Chedin & Kowalczykowski, 2002). Upon interaction with the host-specific sequence w (8 nt in E. coli and 5 nt in B. subtilis), the mediator complex generates a 3 0 -end s ...
arXiv:0708.2724v1 [cond-mat.other] 20 Aug 2007
... have one trait in common: they employ nanoscale probes to examine the structural or electronic signatures of individual DNA bases. That is, they rely on physical differences between the bases. This is a major departure from existing sequencing paradigms which rely on chemical techniques and physical ...
... have one trait in common: they employ nanoscale probes to examine the structural or electronic signatures of individual DNA bases. That is, they rely on physical differences between the bases. This is a major departure from existing sequencing paradigms which rely on chemical techniques and physical ...
RT2 Profiler™ PCR Arrays: Pathway
... 1. Specificity: Using BLAST and other algorithms, the specificity of each primer set is measured against the entire human, mouse, or rat genome to prevent the amplification of sequence-related, non-specific secondary products. The primer specificity is also checked against the E. coli genome to assure th ...
... 1. Specificity: Using BLAST and other algorithms, the specificity of each primer set is measured against the entire human, mouse, or rat genome to prevent the amplification of sequence-related, non-specific secondary products. The primer specificity is also checked against the E. coli genome to assure th ...
Two groups of human herpesvirus 6 identified by sequence
... group A (U 1102) and group B (Z29) show in addition to extensive DNA cross-hybridization an identical genome structure, base composition and size. These are AT-rich, 162 kbp genomes bounded by direct terminal repeats (Martin et al., 1991; Lindquester & Pellett, 1991). In this study we investigated t ...
... group A (U 1102) and group B (Z29) show in addition to extensive DNA cross-hybridization an identical genome structure, base composition and size. These are AT-rich, 162 kbp genomes bounded by direct terminal repeats (Martin et al., 1991; Lindquester & Pellett, 1991). In this study we investigated t ...
Genomic rearrangements account for more than one
... (2201C/T) to evaluate the presence of the mutant transcript. As shown in Figure 2, the DHPLC profile obtained from the RT– PCR of the polymorphic region, lacked the heteroduplex peak present in the genomic DNA as well as in a heterozygous control, suggesting that the putative rearrangement probably ...
... (2201C/T) to evaluate the presence of the mutant transcript. As shown in Figure 2, the DHPLC profile obtained from the RT– PCR of the polymorphic region, lacked the heteroduplex peak present in the genomic DNA as well as in a heterozygous control, suggesting that the putative rearrangement probably ...
Couchioplanes caeruleus - International Journal of Systematic and
... Nacalai Tesque, Inc., Kyoto, Japan); 0.2 M ammonium phosphate-acetonitrile (40:l) was used as the mobile phase. DNA-DNA hybridization. DNA-DNA relatedness was measured fluorometrically by the method of Ezaki et al. (3), using biotinylated DNA. PCR and sequencing of the products. In order to generate ...
... Nacalai Tesque, Inc., Kyoto, Japan); 0.2 M ammonium phosphate-acetonitrile (40:l) was used as the mobile phase. DNA-DNA hybridization. DNA-DNA relatedness was measured fluorometrically by the method of Ezaki et al. (3), using biotinylated DNA. PCR and sequencing of the products. In order to generate ...
... mechanisms are involved in achieving the final RNA levels. One possible explanation for escape of silencing by the paternal Kcnq1 allele is that expression is initiated from an alternative start site. However, we did not observe any alternative transcripts after the transition from monoallelic to bi ...
Molecular cloning of a cDNA encoding a novel Ca2+
... nuclease has recently been found [6], although its function has not been characterized. In eukaryotes, no other homologs have been identi¢ed, and thus the biological function of the plant homolog is of great interest. Here we describe cDNA for staphylococcal nuclease homolog from Arabidopsis thalian ...
... nuclease has recently been found [6], although its function has not been characterized. In eukaryotes, no other homologs have been identi¢ed, and thus the biological function of the plant homolog is of great interest. Here we describe cDNA for staphylococcal nuclease homolog from Arabidopsis thalian ...
Evidence for evolution of canine parvovirus type 2 in Italy
... revealed by this panel of MAbs. The PCR typing approach was also unable to identify the variation that occurred in Glu-426 mutants. Type 2b-specific primers have been designed to take advantage of only two nucleotide variations, and the variation A G at nucleotide 4062, responsible for the change As ...
... revealed by this panel of MAbs. The PCR typing approach was also unable to identify the variation that occurred in Glu-426 mutants. Type 2b-specific primers have been designed to take advantage of only two nucleotide variations, and the variation A G at nucleotide 4062, responsible for the change As ...
Point Mutation in Intron Sequence Causes Altered Carboxyl
... are less susceptible; the other extreme, the hamster, tolerates . 1000-fold higher doses (Henck et al., 1981). There are exceptionally wide differences in susceptibility also within species. Two of these, the divergence between inbred C57BL/6 and DBA/2 mice on the one hand and that between inbred L- ...
... are less susceptible; the other extreme, the hamster, tolerates . 1000-fold higher doses (Henck et al., 1981). There are exceptionally wide differences in susceptibility also within species. Two of these, the divergence between inbred C57BL/6 and DBA/2 mice on the one hand and that between inbred L- ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).