DNA Evidence: Probability, Population Genetics, and the Courts
... difficult for the courts, and those that have generated the most disagreement within the scientific community, involve statistics. The disagreements revolve around one central challenge-presenting the degree of similarity between DNA in a crime sample and DNA in a defendant's sample so that a judge ...
... difficult for the courts, and those that have generated the most disagreement within the scientific community, involve statistics. The disagreements revolve around one central challenge-presenting the degree of similarity between DNA in a crime sample and DNA in a defendant's sample so that a judge ...
Nucleotide Sequence of the DNA Complementary to Avian (Chicken
... Beyond the 1-34 portion of the hormone, the homology was much less apparent. The additional length of the chicken hormone and the difference in sequence could be attributed to at least two deletion and insertion events. The first deletion involves residues 32-44 of the mammalian sequence, which is r ...
... Beyond the 1-34 portion of the hormone, the homology was much less apparent. The additional length of the chicken hormone and the difference in sequence could be attributed to at least two deletion and insertion events. The first deletion involves residues 32-44 of the mammalian sequence, which is r ...
The Plastidic Phosphoglucomutase from
... et al., 1989). Although we could not precisely predict the signal peptide cleavage site, amino acid sequence alignment with other PGMs suggested that the mature protein most likely starts at amino acid 66. If this is true, the molecular mass of the mature protein would be 61 kD, very close to the re ...
... et al., 1989). Although we could not precisely predict the signal peptide cleavage site, amino acid sequence alignment with other PGMs suggested that the mature protein most likely starts at amino acid 66. If this is true, the molecular mass of the mature protein would be 61 kD, very close to the re ...
Competing Interests - Saudi Medical Journal
... Mutation screening and linked markers assessment in HLA locus In order to assess the mutation causing sickle cell and β-thalassaemia in the families, genomic DNA was extracted and exons 1 and 2 (hot-spot area for mutations) of the βglobin (HBB) gene were screened for mutation(s). The primer sequence ...
... Mutation screening and linked markers assessment in HLA locus In order to assess the mutation causing sickle cell and β-thalassaemia in the families, genomic DNA was extracted and exons 1 and 2 (hot-spot area for mutations) of the βglobin (HBB) gene were screened for mutation(s). The primer sequence ...
Analysis of clones carrying repeated DNA sequences in two YAC
... coordinates of the YAC clones which hybridized to chloroplast DNA with the coordinates of clones which had been mapped previously on to chromosome 4 and the top half of chromosome 5 using RFLP markers as probes (Hwang eta/., 1991; Schmidt etal., unpublished results). One-hundred-and-ninety-five clon ...
... coordinates of the YAC clones which hybridized to chloroplast DNA with the coordinates of clones which had been mapped previously on to chromosome 4 and the top half of chromosome 5 using RFLP markers as probes (Hwang eta/., 1991; Schmidt etal., unpublished results). One-hundred-and-ninety-five clon ...
Deoxyribonucleic Acid Base Compositions and Nucleotide
... which contained (per liter) 0.15 g of peptone, 0.15 g of yeast extract, 1 g of glucose, 10 ml of vitamin solution, 20 ml of Hutner basal salts, 250 ml of ASW, 50 ml of Tris, and 0.25 g of (NHJ2S04; the final pH was 7.2. Rhodornicrobium sp. strain P-1093 was grown for 12 days anaerobically at 35°C in ...
... which contained (per liter) 0.15 g of peptone, 0.15 g of yeast extract, 1 g of glucose, 10 ml of vitamin solution, 20 ml of Hutner basal salts, 250 ml of ASW, 50 ml of Tris, and 0.25 g of (NHJ2S04; the final pH was 7.2. Rhodornicrobium sp. strain P-1093 was grown for 12 days anaerobically at 35°C in ...
The Large Loop Repair and Mismatch Repair Pathways
... in the RAD1-dependent LLR pathway springs from the known enzymatic roles of those proteins during mitotic DNA repair and the observed effects on meiotic recombination and DNA repair upon deletion of the LLR genes (Kirkpatrick and Petes 1997). Given the characterized activities of Rad1/10p and Msh2/3 ...
... in the RAD1-dependent LLR pathway springs from the known enzymatic roles of those proteins during mitotic DNA repair and the observed effects on meiotic recombination and DNA repair upon deletion of the LLR genes (Kirkpatrick and Petes 1997). Given the characterized activities of Rad1/10p and Msh2/3 ...
Nutrigenomics in the Patient Care Process: Figuring Out the Puzzle
... Affecting the Body’s Ability to Do Work • SNPs reflect the ability of the body to do work • Some SNPs reduce function, less often enhance function ...
... Affecting the Body’s Ability to Do Work • SNPs reflect the ability of the body to do work • Some SNPs reduce function, less often enhance function ...
Genetics of Down Syndrome
... small structural aberrations with the results of different gene product analyses (dosage effect). Chromosome 21 was sequenced in 2000 (Hattori et al., 2000), and 225 loci (genes) were identified, which was less than expected. This might explain the relatively mild phenotype of the carriers. In the f ...
... small structural aberrations with the results of different gene product analyses (dosage effect). Chromosome 21 was sequenced in 2000 (Hattori et al., 2000), and 225 loci (genes) were identified, which was less than expected. This might explain the relatively mild phenotype of the carriers. In the f ...
Graph-based Methods for the Design of DNA - TUBdok
... DNA computing is a rapidly evolving field utilizing DNA molecules instead of silicon-based electronic units to perform calculations. The reliability of such computations strongly depends on the DNA sequences that represent units of information. Recently, the thermodynamic constraints, based on the f ...
... DNA computing is a rapidly evolving field utilizing DNA molecules instead of silicon-based electronic units to perform calculations. The reliability of such computations strongly depends on the DNA sequences that represent units of information. Recently, the thermodynamic constraints, based on the f ...
Construction and Analysis of 2 Reciprocal Arabidopsis Introgression
... under study, ILs facilitate fine mapping and may lead toward gene discovery (Zamir 2001). The usefulness of ILs in finemapping QTL has already been demonstrated in various plant species (tomato—Frary et al. 2000; Fridman et al. 2000; rice—Takahashi et al. 2001; maize—Salvi et al. 2002; Arabidopsis—E ...
... under study, ILs facilitate fine mapping and may lead toward gene discovery (Zamir 2001). The usefulness of ILs in finemapping QTL has already been demonstrated in various plant species (tomato—Frary et al. 2000; Fridman et al. 2000; rice—Takahashi et al. 2001; maize—Salvi et al. 2002; Arabidopsis—E ...
Chapter 6
... cases. Traditional fingerprinting has been used for more than 100 years, and it has been an extremely important identification tool. Recently, DNA fingerprinting, also called DNA profiling, has started to replace traditional techniques. DNA profiling has been used to clear thousands of wrongly accus ...
... cases. Traditional fingerprinting has been used for more than 100 years, and it has been an extremely important identification tool. Recently, DNA fingerprinting, also called DNA profiling, has started to replace traditional techniques. DNA profiling has been used to clear thousands of wrongly accus ...
Chapter C4
... • Outline the basic steps in making a protein. • Describe three types of mutations, and provide an example of a gene mutation. ...
... • Outline the basic steps in making a protein. • Describe three types of mutations, and provide an example of a gene mutation. ...
Acanthamoeba mitochondrial 16S rDNA sequences: inferred
... becomes more arbitrary and totally depends on the ability to distinguish subgroups on the basis of other characteristics that are consistently reliable. Until the present time, efforts to classify Acanthamoeba species by use of morphology and other methods have been only partially successful. For th ...
... becomes more arbitrary and totally depends on the ability to distinguish subgroups on the basis of other characteristics that are consistently reliable. Until the present time, efforts to classify Acanthamoeba species by use of morphology and other methods have been only partially successful. For th ...
Transposon stability and a role for conjugational transfer in adaptive mutability
... imposed. Conjugal transfer is understood here as episomal DNA transfer from the lacI33-containing bacteria to the scavenger cells as well as self-transfer between lacI33 bacteria. We found that TetR was frequently lost in episomal Lac⫹ revertant colonies that accumulate during selection with and wit ...
... imposed. Conjugal transfer is understood here as episomal DNA transfer from the lacI33-containing bacteria to the scavenger cells as well as self-transfer between lacI33 bacteria. We found that TetR was frequently lost in episomal Lac⫹ revertant colonies that accumulate during selection with and wit ...
Phylogenetic analysis of the insect order Odonata using 28S and
... A phylogenetic analysis of the entire Insecta using 18S rDNA supports the viewpoint of Nel et al. (Kjer 2004); however, Kjer noted that the sequence diversity of this gene was very low in the Odonata, suggesting that his result may not be entirely reliable. Lohmann (1996) provided morphological evid ...
... A phylogenetic analysis of the entire Insecta using 18S rDNA supports the viewpoint of Nel et al. (Kjer 2004); however, Kjer noted that the sequence diversity of this gene was very low in the Odonata, suggesting that his result may not be entirely reliable. Lohmann (1996) provided morphological evid ...
Biophysics 101 Genomics and Computational Biology
... Sugar-binding and crystallographic studies of an arabinose-binding protein mutant (Met108Leu) that exhibits enhanced affinity & altered T7 RNA polymerase mutants with altered promoter specificities. The specificity of carboxypeptidase Y may be altered by changing the hydrophobicity of the S'1 bindin ...
... Sugar-binding and crystallographic studies of an arabinose-binding protein mutant (Met108Leu) that exhibits enhanced affinity & altered T7 RNA polymerase mutants with altered promoter specificities. The specificity of carboxypeptidase Y may be altered by changing the hydrophobicity of the S'1 bindin ...
Towards Programmable Molecular Machines
... Recent related results: Several experimental molecular machines have been recently developed in a laboratory setting. These machines are usually powered either by a natural phenomenon called strand invasion (also known as branch migration) or by enzymes that can break the backbone of DNA at location ...
... Recent related results: Several experimental molecular machines have been recently developed in a laboratory setting. These machines are usually powered either by a natural phenomenon called strand invasion (also known as branch migration) or by enzymes that can break the backbone of DNA at location ...
Metabolism of Selenoamino Acids and Contribution of Selenium
... is metabolized by reduced glutathione (GSH) and/or glutathione reductase to hydrogen selenide (H2Se) via selenocysteine-glutathione selenenyl sulfide (CySeSG). The H2Se is a key intermediate in the methylation process of inorganic and organic selenium compounds. Accumulation of H2Se resulting from i ...
... is metabolized by reduced glutathione (GSH) and/or glutathione reductase to hydrogen selenide (H2Se) via selenocysteine-glutathione selenenyl sulfide (CySeSG). The H2Se is a key intermediate in the methylation process of inorganic and organic selenium compounds. Accumulation of H2Se resulting from i ...
40168_2015_115_MOESM1_ESM
... Chaetomium, Aspergillus, Stachybotrys, which are associated with the worst adverse health effects, produce high amounts of mycotoxins and have therefore been suggested as one of the major causes of the adverse health problems. However, conclusive evidence, including exposure-response-time relationsh ...
... Chaetomium, Aspergillus, Stachybotrys, which are associated with the worst adverse health effects, produce high amounts of mycotoxins and have therefore been suggested as one of the major causes of the adverse health problems. However, conclusive evidence, including exposure-response-time relationsh ...
Screening of Mutations and Polymorphisms in the Glucokinase
... regions in 722 subjects (in 12 patients with an unrecognised type of MODY and their 10 family members, 313 patients with diabetes mellitus type 2 (DM2), 141 gestational diabetics (GDM), 130 healthy offspring of diabetic parents, and 116 healthy controls without family history of DM2). In two MODY fa ...
... regions in 722 subjects (in 12 patients with an unrecognised type of MODY and their 10 family members, 313 patients with diabetes mellitus type 2 (DM2), 141 gestational diabetics (GDM), 130 healthy offspring of diabetic parents, and 116 healthy controls without family history of DM2). In two MODY fa ...
Classification of colorectal cancer based on correlation of clinical
... mutation and CIMP has been shown to be extremely strong in CRC with an odds ratio of over 200.48 While DNA methylation may occur in sporadic adenomas,49 it is seldom marked in small tubular adenomas,50 although it may implicate more loci in adenomas with high-grade dysplasia and ⁄ or villous change. ...
... mutation and CIMP has been shown to be extremely strong in CRC with an odds ratio of over 200.48 While DNA methylation may occur in sporadic adenomas,49 it is seldom marked in small tubular adenomas,50 although it may implicate more loci in adenomas with high-grade dysplasia and ⁄ or villous change. ...
How dormant origins promote complete genome replication
... With these considerations in mind, we recently modelled the behaviour of origin activation within a single 250 kb origin cluster [41]. Origins were assigned a certain initiation probability per unit time and were then activated stochastically during S phase (Figure 4a). Model parameters (mean origin ...
... With these considerations in mind, we recently modelled the behaviour of origin activation within a single 250 kb origin cluster [41]. Origins were assigned a certain initiation probability per unit time and were then activated stochastically during S phase (Figure 4a). Model parameters (mean origin ...
No Slide Title
... cases. Traditional fingerprinting has been used for more than 100 years, and it has been an extremely important identification tool. Recently, DNA fingerprinting, also called DNA profiling, has started to replace traditional techniques. DNA profiling has been used to clear thousands of wrongly accus ...
... cases. Traditional fingerprinting has been used for more than 100 years, and it has been an extremely important identification tool. Recently, DNA fingerprinting, also called DNA profiling, has started to replace traditional techniques. DNA profiling has been used to clear thousands of wrongly accus ...
Sequence requirements for function of the
... gene loci (Asano and Wharton, 1999; Calvi et al., 1998; Royzman et al., 1999). The data demonstrate that Drosophila chorion gene amplification uses evolutionarily conserved machinery for initiation; however, some mechanism must exist to uniquely mark the chorion gene loci origins for activation duri ...
... gene loci (Asano and Wharton, 1999; Calvi et al., 1998; Royzman et al., 1999). The data demonstrate that Drosophila chorion gene amplification uses evolutionarily conserved machinery for initiation; however, some mechanism must exist to uniquely mark the chorion gene loci origins for activation duri ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).