Regulation and Flexibility of Genomic Imprinting
... Institute of Plant Biology and Zürich-Basel Plant Science Center, University of Zürich, CH-8008 Zurich, Switzerland ...
... Institute of Plant Biology and Zürich-Basel Plant Science Center, University of Zürich, CH-8008 Zurich, Switzerland ...
Mitochondrial DNA and aging
... levels of lipophilic carcinogens such as polycyclic aromatic hydrocarbons [22,23]. When cells are exposed to some of these compounds, mtDNA is damaged preferentially [24]. Other mutagenic chemicals also have been shown to preferentially target mtDNA [23,25–29]. Therefore it is conceivable that life- ...
... levels of lipophilic carcinogens such as polycyclic aromatic hydrocarbons [22,23]. When cells are exposed to some of these compounds, mtDNA is damaged preferentially [24]. Other mutagenic chemicals also have been shown to preferentially target mtDNA [23,25–29]. Therefore it is conceivable that life- ...
supporting information
... After denaturation (94oC, 1 min.) and annealing (55oC, 2 min.), PCR overlap extension was carried out (72oC, 5 min.) and the resulting template (4 l) was PCR amplified (standard conditions, 30 cycles), using the relevant oligonucleotides as primers. The shMOG/MS DNA template was generated using o ...
... After denaturation (94oC, 1 min.) and annealing (55oC, 2 min.), PCR overlap extension was carried out (72oC, 5 min.) and the resulting template (4 l) was PCR amplified (standard conditions, 30 cycles), using the relevant oligonucleotides as primers. The shMOG/MS DNA template was generated using o ...
Homology Modelling
... – The highest score hit is often wrong. – Many prediction methods have the correct fold among the top 10-20 hits. – If many different prediction methods all have some fold among the top hits, this fold is ...
... – The highest score hit is often wrong. – Many prediction methods have the correct fold among the top 10-20 hits. – If many different prediction methods all have some fold among the top hits, this fold is ...
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... other primers targeting the nirK gene in Fig. 1. Primers Cunir3 and Cunir4 amplify a larger region than do primers F1aCu and R3Cu (15), similar to primers nirK1F and nirK5R (5). However, Cunir3 and Cunir4 potentially amplify a greater diversity of nirK genes than do primers nirK1F and nirK5R due to ...
... other primers targeting the nirK gene in Fig. 1. Primers Cunir3 and Cunir4 amplify a larger region than do primers F1aCu and R3Cu (15), similar to primers nirK1F and nirK5R (5). However, Cunir3 and Cunir4 potentially amplify a greater diversity of nirK genes than do primers nirK1F and nirK5R due to ...
Effect of RNAi down-regulation of three lysine-deficient
... morphology and physiology of the sorghum plant, its commercial and domestic usage. This chapter also reviews the nutritional deficiency of sorghum grain due to its inherently low content of the essential amino acids lysine and methionine. The chapter concludes by formulating a genetic engineering st ...
... morphology and physiology of the sorghum plant, its commercial and domestic usage. This chapter also reviews the nutritional deficiency of sorghum grain due to its inherently low content of the essential amino acids lysine and methionine. The chapter concludes by formulating a genetic engineering st ...
Structure of a Plasmodium yoelii gene
... A synthetic oligonucleotide M15 was designed to correspond to a highly conserved stretch of seven amino acids, DKTGTKT, encompassing the phosphorylation domain of the aspartyl phosphate cation-transporting ATPases. To investigate whether M15 could reveal specific DNA fragments, genomic DNA of P. yoe ...
... A synthetic oligonucleotide M15 was designed to correspond to a highly conserved stretch of seven amino acids, DKTGTKT, encompassing the phosphorylation domain of the aspartyl phosphate cation-transporting ATPases. To investigate whether M15 could reveal specific DNA fragments, genomic DNA of P. yoe ...
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... tively. We find that the accuracy of FirstEF is significantly higher for CpG-related than non-CpG-related genes. To study the performance of FirstEF on the genome scale, we ran it on human chromosomes 21 and 22 and compared the predictions with the experimentally chromosomes 21 and 22. We downloaded ...
... tively. We find that the accuracy of FirstEF is significantly higher for CpG-related than non-CpG-related genes. To study the performance of FirstEF on the genome scale, we ran it on human chromosomes 21 and 22 and compared the predictions with the experimentally chromosomes 21 and 22. We downloaded ...
VECTOR SYSTEMS XXVII.indd
... Product Description Our Bacillus megaterium kit is a new easy-to-handle system for stable protein expression with high yield. It is not only suited for industrial large scale protein production, but offers also an interesting alternative to the standard host E. coli. The kit comes with the E. coli/B ...
... Product Description Our Bacillus megaterium kit is a new easy-to-handle system for stable protein expression with high yield. It is not only suited for industrial large scale protein production, but offers also an interesting alternative to the standard host E. coli. The kit comes with the E. coli/B ...
Methods for pattern discovery in unaligned biological sequences
... constraints. No pattern length has to be given as input beforehand. The main advantage of this approach is that exhaustive enumeration of the patterns can be avoided, that is, long patterns are built by combining shorter ones. Patterns found by the algorithm are ®nally output ranked according to the ...
... constraints. No pattern length has to be given as input beforehand. The main advantage of this approach is that exhaustive enumeration of the patterns can be avoided, that is, long patterns are built by combining shorter ones. Patterns found by the algorithm are ®nally output ranked according to the ...
Assessment by Molecular Dynamics Simulations of the Structural
... Sp1 consists of three consecutive zinc fingers that recognize a number of nucleotide sequences different from, but related to and sometimes overlapping, those recognized by the structurally better characterized early growth response protein 1 (EGR1, also known as Zif268, Krox-24, and NGFI-A). The ac ...
... Sp1 consists of three consecutive zinc fingers that recognize a number of nucleotide sequences different from, but related to and sometimes overlapping, those recognized by the structurally better characterized early growth response protein 1 (EGR1, also known as Zif268, Krox-24, and NGFI-A). The ac ...
Coordination of replication and transcription along a Drosophila
... 2 (representing 20% of Drosophila euchromatic sequence) with 11,243 nearly contiguous 1.5-kb PCR products. Because almost 90% of the nonrepetitive euchromatic sequence from chromosome 2L is represented on this array, we were able to investigate replication timing at both inter- and intragenic sequen ...
... 2 (representing 20% of Drosophila euchromatic sequence) with 11,243 nearly contiguous 1.5-kb PCR products. Because almost 90% of the nonrepetitive euchromatic sequence from chromosome 2L is represented on this array, we were able to investigate replication timing at both inter- and intragenic sequen ...
Ribosome Profiling
... functional proteins [1]. Unlike DNA inside the cell, transcriptome varies according to the requirement of the cell. Therefore, the study of gene expression by analyzing entire transcripts can provide important insights into the spatial and temporal gene regulation inside a cell. Microarray and RNA s ...
... functional proteins [1]. Unlike DNA inside the cell, transcriptome varies according to the requirement of the cell. Therefore, the study of gene expression by analyzing entire transcripts can provide important insights into the spatial and temporal gene regulation inside a cell. Microarray and RNA s ...
Martina Šeruga Musić, Mladen Krajačić, Dijana Škorić
... For direct amplification of phytoplasma gene or pseudogene regions, three different primer pairs were used: 16R738f/16R1232r (GIBB et al., 1995), to amplify a part of 16S rRNA gene; fTufu/rTufu (SCHNEIDER et al., 1997), to amplify a part of tuf gene; and G35p/m (DAVIS et al., 1992), to amplify dnaB ...
... For direct amplification of phytoplasma gene or pseudogene regions, three different primer pairs were used: 16R738f/16R1232r (GIBB et al., 1995), to amplify a part of 16S rRNA gene; fTufu/rTufu (SCHNEIDER et al., 1997), to amplify a part of tuf gene; and G35p/m (DAVIS et al., 1992), to amplify dnaB ...
A Novel Multigene Family May Encode Odorant Receptors: A
... molecular structure of an odorant can lead to profound changes in perceived odor. How are the diversity and specificity of olfactory perception accomplished? The detection of chemically distinct odorants presumably results from the association of odorous ligands with specific receptors on olfactory ...
... molecular structure of an odorant can lead to profound changes in perceived odor. How are the diversity and specificity of olfactory perception accomplished? The detection of chemically distinct odorants presumably results from the association of odorous ligands with specific receptors on olfactory ...
RecA maintains the integrity of chloroplast DNA molecules in
... a petA probe. Immature, entire shoots of plants grown for 14 d post-germination; mature, first and second rosette leaves from plants grown for 23 d post-germination. The ratio of the hybridization signals for each of the lanes is 6:3.8:1:1.3 for wt immature:cprecA immature:wt mature:cprecA mature, r ...
... a petA probe. Immature, entire shoots of plants grown for 14 d post-germination; mature, first and second rosette leaves from plants grown for 23 d post-germination. The ratio of the hybridization signals for each of the lanes is 6:3.8:1:1.3 for wt immature:cprecA immature:wt mature:cprecA mature, r ...
Lampbrush Chromosomes of the Chicken
... loops (microchromosome in Fig. 1 and Fig. 3 c). A chromosome that we have tentatively identified as a sex chromosome is easily recognized in these preparations as an apparently unpaired chromosome (Fig. 2 and 3). The size of this chromosome is consistent with a tentative identification as the Z chro ...
... loops (microchromosome in Fig. 1 and Fig. 3 c). A chromosome that we have tentatively identified as a sex chromosome is easily recognized in these preparations as an apparently unpaired chromosome (Fig. 2 and 3). The size of this chromosome is consistent with a tentative identification as the Z chro ...
Preparation of single rice chromosome for construction of a DNA
... Fig. 2. (A)–(H) The use of optical scissors and optical trap in isolating individual chromosomes from rice metaphase cells. (A) A rice metaphase cell. The chromosomes were stained with DAPI and shown in bright fluorescence under UV light. The arrows point to the individual chromosomes. (B) The same ...
... Fig. 2. (A)–(H) The use of optical scissors and optical trap in isolating individual chromosomes from rice metaphase cells. (A) A rice metaphase cell. The chromosomes were stained with DAPI and shown in bright fluorescence under UV light. The arrows point to the individual chromosomes. (B) The same ...
Use of a novel cassette to label phenotypically a cryptic plasmid of
... replication A restriction map of pPOD2000 is shown in Fig. 2, using coordinates originating at the unique SalI site. Previous attempts to insert a selectable HindIII fragment into the HindIII sites of pPOD2000 gave insertions at all sites except that at coordinate 2.2 kb, suggesting that this site l ...
... replication A restriction map of pPOD2000 is shown in Fig. 2, using coordinates originating at the unique SalI site. Previous attempts to insert a selectable HindIII fragment into the HindIII sites of pPOD2000 gave insertions at all sites except that at coordinate 2.2 kb, suggesting that this site l ...
2 SINGLE-MOLECULE DNA:PROTEIN INTERACTIONS - VU-dare
... Substantial effort has been spent in pushing the resolution of optical tweezers to allow one of the most elementary events in molecular biology, the stepping of a polymerase enzyme over a single base pair, to be resolved in real time. However, what are the ultimate limits of optical tweezers and wha ...
... Substantial effort has been spent in pushing the resolution of optical tweezers to allow one of the most elementary events in molecular biology, the stepping of a polymerase enzyme over a single base pair, to be resolved in real time. However, what are the ultimate limits of optical tweezers and wha ...
Modular Stitching To Image Single
... The specific example studied in this paper concerns singlemolecule imaging of dynamics internal to polymer chains: the ends and main chain contour of linear DNA. We constructed a hybrid chain assembled from λ-DNA as the parent chain, ∼16 μm (or 48 kbp) in length, and two segments (∼5 kbp), each graft ...
... The specific example studied in this paper concerns singlemolecule imaging of dynamics internal to polymer chains: the ends and main chain contour of linear DNA. We constructed a hybrid chain assembled from λ-DNA as the parent chain, ∼16 μm (or 48 kbp) in length, and two segments (∼5 kbp), each graft ...
Development of Co-Dominant Amplified Polymorphic Sequence
... areas of rice cultivation and is a primary biotic source of yield loss worldwide (15). The utilization of resistant cultivars is the most cost-effective means to mitigate disease losses. However, monogenic resistance commonly is rendered ineffective if frequency of corresponding virulence alleles in ...
... areas of rice cultivation and is a primary biotic source of yield loss worldwide (15). The utilization of resistant cultivars is the most cost-effective means to mitigate disease losses. However, monogenic resistance commonly is rendered ineffective if frequency of corresponding virulence alleles in ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).