Equilibrium Statistics of Channel-confined DNA
... This thesis concerns the statistical description of a very long molecule (a polymer) that is confined to a channel which is much narrower than the size of the molecule. The primary motivation is recent experiments where DNA molecules are inserted into channels with a width of about 100 nm. The resea ...
... This thesis concerns the statistical description of a very long molecule (a polymer) that is confined to a channel which is much narrower than the size of the molecule. The primary motivation is recent experiments where DNA molecules are inserted into channels with a width of about 100 nm. The resea ...
Bioinformatics Variant Analysis
... Most organisms within a particular species differ very little in their genomic structure. These variations are referred to as allele changes. A single nucleotide polymorphism or SNP is a DNA sequence variation occurring when a single nucleotide - A, T, C, or G - in the genome differs between members ...
... Most organisms within a particular species differ very little in their genomic structure. These variations are referred to as allele changes. A single nucleotide polymorphism or SNP is a DNA sequence variation occurring when a single nucleotide - A, T, C, or G - in the genome differs between members ...
Widespread expression of the bovine Agouti gene results from at
... and Ag2 (Fig. 1) to amplify the entire coding region (exons 2, 3 and 4). A single 402 bp fragment was amplified from skin samples of each breed and different tissues (brain, heart, kidney, spleen, lung and liver). PCR fragments were purified and subjected to nucleotide sequence analysis to verify th ...
... and Ag2 (Fig. 1) to amplify the entire coding region (exons 2, 3 and 4). A single 402 bp fragment was amplified from skin samples of each breed and different tissues (brain, heart, kidney, spleen, lung and liver). PCR fragments were purified and subjected to nucleotide sequence analysis to verify th ...
Serum `uracil + uridine` - Journal of Clinical Pathology
... of RNA and the precursor of two of the basesthymine and cytosine-which enter into the composition of DNA. Its main interest, however, is that under certain circumstances it has mutagenic properties (Freese, 1959; Vielmetter and Schuster, 1960; Freese, 1963). The molecular basis of the latter is illu ...
... of RNA and the precursor of two of the basesthymine and cytosine-which enter into the composition of DNA. Its main interest, however, is that under certain circumstances it has mutagenic properties (Freese, 1959; Vielmetter and Schuster, 1960; Freese, 1963). The molecular basis of the latter is illu ...
13-1
... that consists of a long chain of nucleotides. In a general way, genes contain coded DNA instructions that tell cells how to build proteins. The first step in decoding these genetic instructions is to copy part of the base sequence from DNA into RNA. RNA then uses these instructions to direct the prod ...
... that consists of a long chain of nucleotides. In a general way, genes contain coded DNA instructions that tell cells how to build proteins. The first step in decoding these genetic instructions is to copy part of the base sequence from DNA into RNA. RNA then uses these instructions to direct the prod ...
Chapter 17 From Gene to Protein Multiple-Choice Questions
... 7) Using RNA as a template for protein synthesis instead of translating proteins directly from the DNA is advantageous for the cell because A) RNA is much more stable than DNA. B) RNA acts as an expendable copy of the genetic material. C) only one mRNA molecule can be transcribed from a single gene, ...
... 7) Using RNA as a template for protein synthesis instead of translating proteins directly from the DNA is advantageous for the cell because A) RNA is much more stable than DNA. B) RNA acts as an expendable copy of the genetic material. C) only one mRNA molecule can be transcribed from a single gene, ...
document
... Brown midrib (BMR) corn varieties have a natural mutation that results in low lignin in their cell walls, leading to increased digestibility, which is a highly desirable trait for silage maize production. Based on the sequence information from the public database, we designed oligos to amplify the C ...
... Brown midrib (BMR) corn varieties have a natural mutation that results in low lignin in their cell walls, leading to increased digestibility, which is a highly desirable trait for silage maize production. Based on the sequence information from the public database, we designed oligos to amplify the C ...
Genome organization of Magnaporthe grisea
... integrated map showed that the linear order of markers along all seven chromosomes in both maps is in good agreement. Thirty of eighty seven markers were derived from cosmid clones that contained the retrotransposon MAGGY (M. grisea gypsy element). Mapping of singlecopy DNA sequences associated with ...
... integrated map showed that the linear order of markers along all seven chromosomes in both maps is in good agreement. Thirty of eighty seven markers were derived from cosmid clones that contained the retrotransposon MAGGY (M. grisea gypsy element). Mapping of singlecopy DNA sequences associated with ...
slides - DTU CBS
... CENTER FOR BIOLOGICAL SEQUENCE ANALYSIS TECHNICAL UNIVERSITY OF DENMARK DTU ...
... CENTER FOR BIOLOGICAL SEQUENCE ANALYSIS TECHNICAL UNIVERSITY OF DENMARK DTU ...
DNA Polyacrylamide Gel Electrophoresis
... Stock solutions other than 29:1 (% w/v) acrylamide:bisacrylamide can be used to cast polyacrylamide gels. However, it is then necessary to recalculate the appropriate amount of stock solution to use. Gels can be cast with acrylamide solutions containing different acrylamide:bisacrylamide (cross-link ...
... Stock solutions other than 29:1 (% w/v) acrylamide:bisacrylamide can be used to cast polyacrylamide gels. However, it is then necessary to recalculate the appropriate amount of stock solution to use. Gels can be cast with acrylamide solutions containing different acrylamide:bisacrylamide (cross-link ...
Illustrating Python via Bioinformatics Examples
... Department of Informatics, University of Oslo ...
... Department of Informatics, University of Oslo ...
Bacteriophage A cloning system for the construction of
... of genes isolated by cDNA cloning techniques. Since their introduction (1, 2) these techniques have been refined to allow for the isolation of eukaryotic genes expressed at low frequencies. Oligonucleotide probes or antibodies to eukaryotic gene products are widely used for screening bacterial libra ...
... of genes isolated by cDNA cloning techniques. Since their introduction (1, 2) these techniques have been refined to allow for the isolation of eukaryotic genes expressed at low frequencies. Oligonucleotide probes or antibodies to eukaryotic gene products are widely used for screening bacterial libra ...
Genome-Wide Analysis of Natural Selection on
... disparate ascertainment conditions to different SNPs, we chose not to compare the DAF distributions of foreground sites with neutral models such as Tajima’s D or Fay and Wu’s H, following the precedent of[12]. Instead, as in[11,12], for each population we compared the DAF distribution of the foregro ...
... disparate ascertainment conditions to different SNPs, we chose not to compare the DAF distributions of foreground sites with neutral models such as Tajima’s D or Fay and Wu’s H, following the precedent of[12]. Instead, as in[11,12], for each population we compared the DAF distribution of the foregro ...
1 - CiteSeerX
... and it was estimated that the maximal content of cytosine in T6r+, for instance, was 0.2 per cent of its content of HMC. Conversely, analysis of the bacterial host and of its DNA did not detect HMC and the maximal HMC contents of these ~aterials were estimated to be 0.2 and 0.6 per cent respectively ...
... and it was estimated that the maximal content of cytosine in T6r+, for instance, was 0.2 per cent of its content of HMC. Conversely, analysis of the bacterial host and of its DNA did not detect HMC and the maximal HMC contents of these ~aterials were estimated to be 0.2 and 0.6 per cent respectively ...
Aminoacyl-tRNA Synthetase Production for Unnatural Amino Acid
... innovative tool for accomplishing these aims is cell-free protein synthesis (CFPS). This technique, rather than using living cells to make protein, simply extracts the cells’ natural protein-making machinery and then uses it to produce protein in vitro. Because living cells are no longer involved, s ...
... innovative tool for accomplishing these aims is cell-free protein synthesis (CFPS). This technique, rather than using living cells to make protein, simply extracts the cells’ natural protein-making machinery and then uses it to produce protein in vitro. Because living cells are no longer involved, s ...
The Use of Cytochrome B Sequence Variation in Estimation of
... H,O up to a final volume of 100 ~1 and covered by 75 pul of mineral oil. The cyclesfor the asymmetric reaction were identical to the initial PCR except the middle amplification cycles were repeated 40 times. For every sample, one ofthe two isolated products was used as a template to produce a specif ...
... H,O up to a final volume of 100 ~1 and covered by 75 pul of mineral oil. The cyclesfor the asymmetric reaction were identical to the initial PCR except the middle amplification cycles were repeated 40 times. For every sample, one ofthe two isolated products was used as a template to produce a specif ...
![Crystal structures of -[Ru(phen)2dppz]2+ 1 with oligonucleotides](http://s1.studyres.com/store/data/023790436_1-6c83654c03a0a35fc50303cc056eabf6-300x300.png)
Crystal structures of -[Ru(phen)2dppz]2+ 1 with oligonucleotides
... This self-complementary sequence crystallizes to give a symmetrical duplex with a stoichiometry of three cations of 1 per duplex, or 1.5 cations of 1 per decamer strand. All the nucleic acid strands are equivalent in the crystal lattice, with the packing shown in Figure 1a. The conformation of a sin ...
... This self-complementary sequence crystallizes to give a symmetrical duplex with a stoichiometry of three cations of 1 per duplex, or 1.5 cations of 1 per decamer strand. All the nucleic acid strands are equivalent in the crystal lattice, with the packing shown in Figure 1a. The conformation of a sin ...
DNA sequence of the rat growth hormone gene: location of the 5
... Digestions were usually done with a s u b s t a n t i a l excess of enzyme and approximately in accordance with t h e conditions provided by t h e manufact u r e r . Gel e l e c t r o p h o r e t i c separations, unless specified otherwise, were performed e i t h e r on 1% agarose gels or fi% acryla ...
... Digestions were usually done with a s u b s t a n t i a l excess of enzyme and approximately in accordance with t h e conditions provided by t h e manufact u r e r . Gel e l e c t r o p h o r e t i c separations, unless specified otherwise, were performed e i t h e r on 1% agarose gels or fi% acryla ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).